BuxeA.00024.e
Putative alpha KG dependent 2,4-D dioxygenase
| CENTER ID: | BuxeA.00024.e |
| ORGANISM: | Burkholderia xenovorans LB400 |
| ASSOCIATED DISEASE: | |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | True |
| NIH RISK GROUP: | 3 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
IIIB |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| BuxeA.00024.e.B1.GE39345 | Full length( BuxeA.00024.e ) | 1 | 301 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| BuxeA.00024.e.B1.PS02518 | Full length( BuxeA.00024.e ) | 1 | 301 |
Structures
5J92
DEPOSITED: 4/8/2016
DETERMINATION: XRay
CLONE: BuxeA.00024.e.B1.GE39345
PROTEIN: BuxeA.00024.e.B1.PS02518
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| OrthoMCL: | OG5_155768 |
| PATRIC ID: | fig|266265.5.peg.5575 |
| RefSeq: | YP_553184.1 |
| UniProt: | Q13Q05 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSLNVEAAHP FIAARIHGLD LSKPLSDERI VEIEQASGQY PVLIFPRQYI DDDQLLAFAA GFGPLQVAVS YSTRPEDHRL APMINDISNL SKENQTYRPG DRRRMNNLTS RRWHSDASYL PLPARYSFLL SYIVPAVGGQ TQFADMRAAY DKLPDHLRKV VEGLSCHYDI MASRAAAGFY DASDEERKAL APCIHELVRT HPISGRKSLY LSSHATHVVG WPEPEGRDLL RELTEFATQP QFVYSHEWSV RDLVMWDNRA LMHRGRPHIP ETDVREMHRA TTLDDRTWTR GSNQPVAASV A
NT Sequence
ATGAGCCTGA ACGTCGAAGC CGCCCATCCT TTCATCGCCG CGCGCATCCA CGGACTCGAT CTGTCAAAAC CGCTTTCCGA TGAGCGTATC GTCGAAATCG AGCAGGCCAG CGGCCAATAC CCGGTGCTGA TCTTCCCGCG CCAGTACATC GACGACGACC AGCTGCTTGC ATTCGCCGCC GGCTTCGGTC CGCTGCAGGT TGCCGTCTCG TACTCGACGC GCCCGGAGGA TCACCGGCTC GCCCCGATGA TCAACGACAT CTCCAATCTC AGCAAGGAGA ACCAGACCTA CCGGCCCGGT GACCGGCGCA GGATGAACAA TCTCACGAGC CGCCGCTGGC ATTCGGATGC TTCGTATCTG CCGCTGCCCG CACGCTATTC GTTTCTGCTC TCGTATATCG TGCCGGCGGT CGGCGGTCAG ACGCAGTTCG CCGACATGCG CGCCGCGTAT GACAAGCTGC CCGACCATTT ACGCAAGGTT GTCGAAGGCC TGAGCTGCCA CTACGACATC ATGGCGTCAC GCGCGGCGGC CGGCTTCTAC GACGCATCCG ACGAAGAACG CAAGGCGCTC GCGCCGTGCA TTCACGAACT GGTCCGCACG CATCCCATCT CTGGACGCAA ATCGCTGTAC CTGTCGAGCC ACGCGACGCA TGTGGTCGGC TGGCCCGAGC CGGAGGGCCG CGATCTGCTG CGCGAACTGA CCGAGTTCGC CACGCAGCCG CAGTTCGTGT ATTCGCACGA ATGGTCGGTT CGCGACCTCG TCATGTGGGA CAACCGTGCG CTGATGCATC GCGGCCGCCC GCATATTCCG GAAACCGACG TTCGCGAGAT GCATCGCGCA ACGACGCTCG ACGATCGCAC CTGGACGCGC GGCAGCAATC AGCCGGTCGC GGCAAGCGTC GCC
Details for BuxeA.00024.e.B1.GE39345
| HARVESTED ON: | 7/10/2015 |
| SEQUENCED ON: | 7/6/2015 |
| EXPECTED MW: | 35kDa |
| OBSERVED MW: | 35kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Too many to count (100+) |
| TOTAL EXPRESSION LEVEL: | Moderate Expression |
| SOLUBLE EXPRESSION LEVEL | Moderate Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 100 |
Validated AA Sequence
MAHHHHHHMS LNVEAAHPFI AARIHGLDLS KPLSDERIVE IEQASGQYPV LIFPRQYIDD DQLLAFAAGF GPLQVAVSYS TRPEDHRLAP MINDISNLSK ENQTYRPGDR RRMNNLTSRR WHSDASYLPL PARYSFLLSY IVPAVGGQTQ FADMRAAYDK LPDHLRKVVE GLSCHYDIMA SRAAAGFYDA SDEERKALAP CIHELVRTHP ISGRKSLYLS SHATHVVGWP EPEGRDLLRE LTEFATQPQF VYSHEWSVRD LVMWDNRALM HRGRPHIPET DVREMHRATT LDDRTWTRGS NQPVAASVA
Validated NT Sequence
atcttactca cttaggcgac gcttgccgcg accggctgat tgctgccgcg cgtccaggtg cgatcgtcga gcgtcgttgc gcgatgcatc tcgcgaacgt cggtttccgg aatatgcggg cggccgcgat gcatcagcgc acggttgtcc cacatgacga ggtcgcgaac cgaccattcg tgcgaataca cgaactgcgg ctgcgtggcg aactcggtca gttcgcgcag cagatcgcgg ccctccggct cgggccagcc gaccacatgc gtcgcgtggc tcgacaggta cagcgatttg cgtccagaga tgggatgcgt gcggaccagt tcgtgaatgc acggcgcgag cgccttgcgt tcttcgtcgg atgcgtcgta gaagccggcc gccgcgcgtg acgccatgat gtcgtagtgg cagctcaggc cttcgacaac cttgcgtaaa tggtcgggca gcttgtcata cgcggcgcgc atgtcggcga actgcgtctg accgccgacc gccggcacga tatacgagag cagaaacgaa tagcgtgcgg gcagcggcag atacgaagca tccgaatgcc agcggcggct cgtgagattg ttcatcctgc gccggtcacc gggccggtag gtctggttct ccttgctgag attggagatg tcgttgatca tcggggcgag ccggtgatcc tccgggcgcg tcgagtacga gacggcaacc tgcagcggac cgaagccggc ggcgaatgca agcagctggt cgtcgtcgat gtactggcgc gggaagatca gcaccgggta ttggccgctg gcctgctcga tttcgacgat acgctcatcg gaaagcggtt ttgacagatc gagtccgtgg atgcgcgcgg cgatgaaagg atgggcggct tcgacgttca ggctcatatg gtggtggtgg tggtgagcca t
Expected Protein Sequence
MAHHHHHHMS LNVEAAHPFI AARIHGLDLS KPLSDERIVE IEQASGQYPV LIFPRQYIDD DQLLAFAAGF GPLQVAVSYS TRPEDHRLAP MINDISNLSK ENQTYRPGDR RRMNNLTSRR WHSDASYLPL PARYSFLLSY IVPAVGGQTQ FADMRAAYDK LPDHLRKVVE GLSCHYDIMA SRAAAGFYDA SDEERKALAP CIHELVRTHP ISGRKSLYLS SHATHVVGWP EPEGRDLLRE LTEFATQPQF VYSHEWSVRD LVMWDNRALM HRGRPHIPET DVREMHRATT LDDRTWTRGS NQPVAASVA
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgagcc tgaacgtcga agccgcccat cctttcatcg ccgcgcgcat ccacggactc gatctgtcaa aaccgctttc cgatgagcgt atcgtcgaaa tcgagcaggc cagcggccaa tacccggtgc tgatcttccc gcgccagtac atcgacgacg accagctgct tgcattcgcc gccggcttcg gtccgctgca ggttgccgtc tcgtactcga cgcgcccgga ggatcaccgg ctcgccccga tgatcaacga catctccaat ctcagcaagg agaaccagac ctaccggccc ggtgaccggc gcaggatgaa caatctcacg agccgccgct ggcattcgga tgcttcgtat ctgccgctgc ccgcacgcta ttcgtttctg ctctcgtata tcgtgccggc ggtcggcggt cagacgcagt tcgccgacat gcgcgccgcg tatgacaagc tgcccgacca tttacgcaag gttgtcgaag gcctgagctg ccactacgac atcatggcgt cacgcgcggc ggccggcttc tacgacgcat ccgacgaaga acgcaaggcg ctcgcgccgt gcattcacga actggtccgc acgcatccca tctctggacg caaatcgctg tacctgtcga gccacgcgac gcatgtggtc ggctggcccg agccggaggg ccgcgatctg ctgcgcgaac tgaccgagtt cgccacgcag ccgcagttcg tgtattcgca cgaatggtcg gttcgcgacc tcgtcatgtg ggacaaccgt gcgctgatgc atcgcggccg cccgcatatt ccggaaaccg acgttcgcga gatgcatcgc gcaacgacgc tcgacgatcg cacctggacg cgcggcagca atcagccggt cgcggcaagc gtcgcctgag taagatagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aattcttgaa gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgcagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat acactccgct atcgctacgt gactgggtca tggctgcgcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgaggcagc tgcggtaaag ctcatcagcg tggtcgtgaa gcgattcaca gatgtctgcc tgttcatccg cgtccagctc gttgagtttc tccagaagcg ttaatgtctg gcttctgata aagcgggcca tgttaagggc ggttttttcc tgtttggtca ctgatgcctc cgtgtaaggg ggatttctgt tcatgggggt aatgataccg atgaaacgag agaggatgct cacgatacgg gttactgatg atgaacatgc ccggttactg gaacgttgtg agggtaaaca actggcggta tggatgcggc gggaccagag aaaaatcact cagggtcaat gccagcgctt cgttaataca gatgtaggtg ttccacaggg tagccagcag catcctgcga tgcagatccg gaacataatg gtgcagggcg ctgacttccg cgtttccaga ctttacgaaa cacggaaacc gaagaccatt catgttgttg ctcaggtcgc agacgttttg cagcagcagt cgcttcacgt tcgctcgcgt atcggtgatt cattctgcta accagtaagg caaccccgcc agcctagccg ggtcctcaac gacaggagca cgatcatgcg cacccgtggc caggacccaa cgctgcccga gatgcgccgc gtgcggctgc tggagatggc ggacgcgatg gatatgttct gccaagggtt ggtttgcgca ttcacagttc tccgcaagaa ttgattggct ccaattcttg gagtggtgaa tccgttagcg aggtgccgcc ggcttccatt caggtcgagg tggcccggct ccatgcaccg cgacgcaacg cggggaggca gacaaggtat agggcggcgc ctacaatcca tgccaacccg ttccatgtgc tcgccgaggc ggcataaatc gccgtgacga tcagcggtcc agtgatcgaa gttaggctgg taagagccgc gagcgatcct tgaagctgtc cctgatggtc gtcatctacc tgcctggaca gcatggcctg caacgcgggc atcccgatgc cgccggaagc gagaagaatc ataatgggga aggccatcca gcctcgcgtc gcgaacgcca gcaagacgta gcccagcgcg tcggccgcca tgccggcgat aatggcctgc ttctcgccga aacgtttggt ggcgggacca gtgacgaagg cttgagcgag ggcgtgcaag attccgaata ccgcaagcga caggccgatc atcgtcgcgc tccagcgaaa gcggtcctcg ccgaaaatga cccagagcgc tgccggcacc tgtcctacga gttgcatgat aaagaagaca gtcataagtg cggcgacgat agtcatgccc cgcgcccacc ggaaggagct gactgggttg aaggctctca agggcatcgg tcgacgctct cccttatgcg actcctgcat taggaagcag cccagtagta ggttgaggcc gttgagcacc gccgccgcaa ggaatggtgc atgcaaggag atggcgccca acagtccccc ggccacgggg cctgccacca tacccacgcc gaaacaagcg ctcatgagcc cgaagtggcg agcccgatct tccccatcgg tgatgtcggc gatataggcg ccagcaaccg cacctgtggc gccggtgatg ccggccacga tgcgtccggc gtagaggatc gagatctcga tcccgcgaaa t
Details for BuxeA.00024.e.B1.PS02518
| PURIFICATION DATe: | 10/15/2015 |
| CONCENTRATION: | 28.7mg/ml |
| OBSERVED MW: | 36kDa |
| EXPRESSION LEVEL: | Moderate Expression |
| PROTEIN PURIFICATION BUFFER: | SEC buffer |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 3 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 100 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMS LNVEAAHPFI AARIHGLDLS KPLSDERIVE IEQASGQYPV LIFPRQYIDD DQLLAFAAGF GPLQVAVSYS TRPEDHRLAP MINDISNLSK ENQTYRPGDR RRMNNLTSRR WHSDASYLPL PARYSFLLSY IVPAVGGQTQ FADMRAAYDK LPDHLRKVVE GLSCHYDIMA SRAAAGFYDA SDEERKALAP CIHELVRTHP ISGRKSLYLS SHATHVVGWP EPEGRDLLRE LTEFATQPQF VYSHEWSVRD LVMWDNRALM HRGRPHIPET DVREMHRATT LDDRTWTRGS NQPVAASVA
Validated NT Sequence
atcttactca cttaggcgac gcttgccgcg accggctgat tgctgccgcg cgtccaggtg cgatcgtcga gcgtcgttgc gcgatgcatc tcgcgaacgt cggtttccgg aatatgcggg cggccgcgat gcatcagcgc acggttgtcc cacatgacga ggtcgcgaac cgaccattcg tgcgaataca cgaactgcgg ctgcgtggcg aactcggtca gttcgcgcag cagatcgcgg ccctccggct cgggccagcc gaccacatgc gtcgcgtggc tcgacaggta cagcgatttg cgtccagaga tgggatgcgt gcggaccagt tcgtgaatgc acggcgcgag cgccttgcgt tcttcgtcgg atgcgtcgta gaagccggcc gccgcgcgtg acgccatgat gtcgtagtgg cagctcaggc cttcgacaac cttgcgtaaa tggtcgggca gcttgtcata cgcggcgcgc atgtcggcga actgcgtctg accgccgacc gccggcacga tatacgagag cagaaacgaa tagcgtgcgg gcagcggcag atacgaagca tccgaatgcc agcggcggct cgtgagattg ttcatcctgc gccggtcacc gggccggtag gtctggttct ccttgctgag attggagatg tcgttgatca tcggggcgag ccggtgatcc tccgggcgcg tcgagtacga gacggcaacc tgcagcggac cgaagccggc ggcgaatgca agcagctggt cgtcgtcgat gtactggcgc gggaagatca gcaccgggta ttggccgctg gcctgctcga tttcgacgat acgctcatcg gaaagcggtt ttgacagatc gagtccgtgg atgcgcgcgg cgatgaaagg atgggcggct tcgacgttca ggctcatatg gtggtggtgg tggtgagcca t
Expressed Protein Sequence
MAHHHHHHMS LNVEAAHPFI AARIHGLDLS KPLSDERIVE IEQASGQYPV LIFPRQYIDD DQLLAFAAGF GPLQVAVSYS TRPEDHRLAP MINDISNLSK ENQTYRPGDR RRMNNLTSRR WHSDASYLPL PARYSFLLSY IVPAVGGQTQ FADMRAAYDK LPDHLRKVVE GLSCHYDIMA SRAAAGFYDA SDEERKALAP CIHELVRTHP ISGRKSLYLS SHATHVVGWP EPEGRDLLRE LTEFATQPQF VYSHEWSVRD LVMWDNRALM HRGRPHIPET DVREMHRATT LDDRTWTRGS NQPVAASVA
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGAGCC TGAACGTCGA AGCCGCCCAT CCTTTCATCG CCGCGCGCAT CCACGGACTC GATCTGTCAA AACCGCTTTC CGATGAGCGT ATCGTCGAAA TCGAGCAGGC CAGCGGCCAA TACCCGGTGC TGATCTTCCC GCGCCAGTAC ATCGACGACG ACCAGCTGCT TGCATTCGCC GCCGGCTTCG GTCCGCTGCA GGTTGCCGTC TCGTACTCGA CGCGCCCGGA GGATCACCGG CTCGCCCCGA TGATCAACGA CATCTCCAAT CTCAGCAAGG AGAACCAGAC CTACCGGCCC GGTGACCGGC GCAGGATGAA CAATCTCACG AGCCGCCGCT GGCATTCGGA TGCTTCGTAT CTGCCGCTGC CCGCACGCTA TTCGTTTCTG CTCTCGTATA TCGTGCCGGC GGTCGGCGGT CAGACGCAGT TCGCCGACAT GCGCGCCGCG TATGACAAGC TGCCCGACCA TTTACGCAAG GTTGTCGAAG GCCTGAGCTG CCACTACGAC ATCATGGCGT CACGCGCGGC GGCCGGCTTC TACGACGCAT CCGACGAAGA ACGCAAGGCG CTCGCGCCGT GCATTCACGA ACTGGTCCGC ACGCATCCCA TCTCTGGACG CAAATCGCTG TACCTGTCGA GCCACGCGAC GCATGTGGTC GGCTGGCCCG AGCCGGAGGG CCGCGATCTG CTGCGCGAAC TGACCGAGTT CGCCACGCAG CCGCAGTTCG TGTATTCGCA CGAATGGTCG GTTCGCGACC TCGTCATGTG GGACAACCGT GCGCTGATGC ATCGCGGCCG CCCGCATATT CCGGAAACCG ACGTTCGCGA GATGCATCGC GCAACGACGC TCGACGATCG CACCTGGACG CGCGGCAGCA ATCAGCCGGT CGCGGCAAGC GTCGCCTGAG TAAGATAGGA TCCGGCTGCT AACAAAGCCC GAAAGGAAGC TGAGTTGGCT GCTGCCACCG CTGAGCAATA ACTAGCATAA CCCCTTGGGG CCTCTAAACG GGTCTTGAGG GGTTTTTTGC TGAAAGGAGG AACTATATCC GGATATCCAC AGGACGGGTG TGGTCGCCAT GATCGCGTAG TCGATAGTGG CTCCAAGTAG CGAAGCGAGC AGGACTGGGC GGCGGCCAAA GCGGTCGGAC AGTGCTCCGA GAACGGGTGC GCATAGAAAT TGCATCAACG CATATAGCGC TAGCAGCACG CCATAGTGAC TGGCGATGCT GTCGGAATGG ACGATATCCC GCAAGAGGCC CGGCAGTACC GGCATAACCA AGCCTATGCC TACAGCATCC AGGGTGACGG TGCCGAGGAT GACGATGAGC GCATTGTTAG ATTTCATACA CGGTGCCTGA CTGCGTTAGC AATTTAACTG TGATAAACTA CCGCATTAAA GCTTATCGAT GATAAGCTGT CAAACATGAG AATTCTTGAA GACGAAAGGG CCTCGTGATA CGCCTATTTT TATAGGTTAA TGTCATGATA ATAATGGTTT CTTAGACGTC AGGTGGCACT TTTCGGGGAA ATGTGCGCGG AACCCCTATT TGTTTATTTT TCTAAATACA TTCAAATATG TATCCGCTCA TGAGACAATA ACCCTGATAA ATGCTTCAAT AATATTGAAA AAGGAAGAGT ATGAGTATTC AACATTTCCG TGTCGCCCTT ATTCCCTTTT TTGCGGCATT TTGCCTTCCT GTTTTTGCTC ACCCAGAAAC GCTGGTGAAA GTAAAAGATG CTGAAGATCA GTTGGGTGCA CGAGTGGGTT ACATCGAACT GGATCTCAAC AGCGGTAAGA TCCTTGAGAG TTTTCGCCCC GAAGAACGTT TTCCAATGAT GAGCACTTTT AAAGTTCTGC TATGTGGCGC GGTATTATCC CGTGTTGACG CCGGGCAAGA GCAACTCGGT CGCCGCATAC ACTATTCTCA GAATGACTTG GTTGAGTACT CACCAGTCAC AGAAAAGCAT CTTACGGATG GCATGACAGT AAGAGAATTA TGCAGTGCTG CCATAACCAT GAGTGATAAC ACTGCGGCCA ACTTACTTCT GACAACGATC GGAGGACCGA AGGAGCTAAC CGCTTTTTTG CACAACATGG GGGATCATGT AACTCGCCTT GATCGTTGGG AACCGGAGCT GAATGAAGCC ATACCAAACG ACGAGCGTGA CACCACGATG CCTGCAGCAA TGGCAACAAC GTTGCGCAAA CTATTAACTG GCGAACTACT TACTCTAGCT TCCCGGCAAC AATTAATAGA CTGGATGGAG GCGGATAAAG TTGCAGGACC ACTTCTGCGC TCGGCCCTTC CGGCTGGCTG GTTTATTGCT GATAAATCTG GAGCCGGTGA GCGTGGGTCT CGCGGTATCA TTGCAGCACT GGGGCCAGAT GGTAAGCCCT CCCGTATCGT AGTTATCTAC ACGACGGGGA GTCAGGCAAC TATGGATGAA CGAAATAGAC AGATCGCTGA GATAGGTGCC TCACTGATTA AGCATTGGTA ACTGTCAGAC CAAGTTTACT CATATATACT TTAGATTGAT TTAAAACTTC ATTTTTAATT TAAAAGGATC TAGGTGAAGA TCCTTTTTGA TAATCTCATG ACCAAAATCC CTTAACGTGA GTTTTCGTTC CACTGAGCGT CAGACCCCGT AGAAAAGATC AAAGGATCTT CTTGAGATCC TTTTTTTCTG CGCGTAATCT GCTGCTTGCA AACAAAAAAA CCACCGCTAC CAGCGGTGGT TTGTTTGCCG GATCAAGAGC TACCAACTCT TTTTCCGAAG GTAACTGGCT TCAGCAGAGC GCAGATACCA AATACTGTCC TTCTAGTGTA GCCGTAGTTA GGCCACCACT TCAAGAACTC TGTAGCACCG CCTACATACC TCGCTCTGCT AATCCTGTTA CCAGTGGCTG CTGCCAGTGG CGATAAGTCG TGTCTTACCG GGTTGGACTC AAGACGATAG TTACCGGATA AGGCGCAGCG GTCGGGCTGA ACGGGGGGTT CGTGCACACA GCCCAGCTTG GAGCGAACGA CCTACACCGA ACTGAGATAC CTACAGCGTG AGCTATGAGA AAGCGCCACG CTTCCCGAAG GGAGAAAGGC GGACAGGTAT CCGGTAAGCG GCAGGGTCGG AACAGGAGAG CGCACGAGGG AGCTTCCAGG GGGAAACGCC TGGTATCTTT ATAGTCCTGT CGGGTTTCGC CACCTCTGAC TTGAGCGTCG ATTTTTGTGA TGCTCGTCAG GGGGGCGGAG CCTATGGAAA AACGCCAGCA ACGCGGCCTT TTTACGGTTC CTGGCCTTTT GCTGGCCTTT TGCTCACATG TTCTTTCCTG CGTTATCCCC TGATTCTGTG GATAACCGTA TTACCGCCTT TGAGTGAGCT GATACCGCTC GCCGCAGCCG AACGACCGAG CGCAGCGAGT CAGTGAGCGA GGAAGCGGAA GAGCGCCTGA TGCGGTATTT TCTCCTTACG CATCTGTGCG GTATTTCACA CCGCATATAT GGTGCACTCT CAGTACAATC TGCTCTGATG CCGCATAGTT AAGCCAGTAT ACACTCCGCT ATCGCTACGT GACTGGGTCA TGGCTGCGCC CCGACACCCG CCAACACCCG CTGACGCGCC CTGACGGGCT TGTCTGCTCC CGGCATCCGC TTACAGACAA GCTGTGACCG TCTCCGGGAG CTGCATGTGT CAGAGGTTTT CACCGTCATC ACCGAAACGC GCGAGGCAGC TGCGGTAAAG CTCATCAGCG TGGTCGTGAA GCGATTCACA GATGTCTGCC TGTTCATCCG CGTCCAGCTC GTTGAGTTTC TCCAGAAGCG TTAATGTCTG GCTTCTGATA AAGCGGGCCA TGTTAAGGGC GGTTTTTTCC TGTTTGGTCA CTGATGCCTC CGTGTAAGGG GGATTTCTGT TCATGGGGGT AATGATACCG ATGAAACGAG AGAGGATGCT CACGATACGG GTTACTGATG ATGAACATGC CCGGTTACTG GAACGTTGTG AGGGTAAACA ACTGGCGGTA TGGATGCGGC GGGACCAGAG AAAAATCACT CAGGGTCAAT GCCAGCGCTT CGTTAATACA GATGTAGGTG TTCCACAGGG TAGCCAGCAG CATCCTGCGA TGCAGATCCG GAACATAATG GTGCAGGGCG CTGACTTCCG CGTTTCCAGA CTTTACGAAA CACGGAAACC GAAGACCATT CATGTTGTTG CTCAGGTCGC AGACGTTTTG CAGCAGCAGT CGCTTCACGT TCGCTCGCGT ATCGGTGATT CATTCTGCTA ACCAGTAAGG CAACCCCGCC AGCCTAGCCG GGTCCTCAAC GACAGGAGCA CGATCATGCG CACCCGTGGC CAGGACCCAA CGCTGCCCGA GATGCGCCGC GTGCGGCTGC TGGAGATGGC GGACGCGATG GATATGTTCT GCCAAGGGTT GGTTTGCGCA TTCACAGTTC TCCGCAAGAA TTGATTGGCT CCAATTCTTG GAGTGGTGAA TCCGTTAGCG AGGTGCCGCC GGCTTCCATT CAGGTCGAGG TGGCCCGGCT CCATGCACCG CGACGCAACG CGGGGAGGCA GACAAGGTAT AGGGCGGCGC CTACAATCCA TGCCAACCCG TTCCATGTGC TCGCCGAGGC GGCATAAATC GCCGTGACGA TCAGCGGTCC AGTGATCGAA GTTAGGCTGG TAAGAGCCGC GAGCGATCCT TGAAGCTGTC CCTGATGGTC GTCATCTACC TGCCTGGACA GCATGGCCTG CAACGCGGGC ATCCCGATGC CGCCGGAAGC GAGAAGAATC ATAATGGGGA AGGCCATCCA GCCTCGCGTC GCGAACGCCA GCAAGACGTA GCCCAGCGCG TCGGCCGCCA TGCCGGCGAT AATGGCCTGC TTCTCGCCGA AACGTTTGGT GGCGGGACCA GTGACGAAGG CTTGAGCGAG GGCGTGCAAG ATTCCGAATA CCGCAAGCGA CAGGCCGATC ATCGTCGCGC TCCAGCGAAA GCGGTCCTCG CCGAAAATGA CCCAGAGCGC TGCCGGCACC TGTCCTACGA GTTGCATGAT AAAGAAGACA GTCATAAGTG CGGCGACGAT AGTCATGCCC CGCGCCCACC GGAAGGAGCT GACTGGGTTG AAGGCTCTCA AGGGCATCGG TCGACGCTCT CCCTTATGCG ACTCCTGCAT TAGGAAGCAG CCCAGTAGTA GGTTGAGGCC GTTGAGCACC GCCGCCGCAA GGAATGGTGC ATGCAAGGAG ATGGCGCCCA ACAGTCCCCC GGCCACGGGG CCTGCCACCA TACCCACGCC GAAACAAGCG CTCATGAGCC CGAAGTGGCG AGCCCGATCT TCCCCATCGG TGATGTCGGC GATATAGGCG CCAGCAACCG CACCTGTGGC GCCGGTGATG CCGGCCACGA TGCGTCCGGC GTAGAGGATC GAGATCTCGA TCCCGCGAAA T