BuxeA.00024.d

Putative alpha KG dependent 2,4-D dioxygenase

CENTER ID: BuxeA.00024.d
ORGANISM: Burkholderia xenovorans LB400
ASSOCIATED DISEASE:
CURRENT STATUS: purified
COMMUNITY REQUEST: False
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BuxeA.00024.d.B1.GE39344 Full length( BuxeA.00024.d ) 1 298
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|266265.5.peg.5588
RefSeq: YP_553195.1
UniProt: Q13PZ4

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSLDIEPAHP TIAARIRGLD LRQPLSDEQV DEIGQASAIY PVLIFPNQLI DDAQLMAFSQ NFGPLQPVVS FHTAKADHRL SPMVSDISNL DKNNRTFPAG DRRRMNFLSS RRWHTDGSYL PTPNRYSMLL AYTVARVGGQ TQFADMRAAY DALPAEWLEL VEDLTLEHNV MHSRAVAGFT DFDEEERRRF PATHKKLVRR HPVSGRLSLY LSGHASHVVG WPVPEGLDLL RELTEFATQP QFVYTHEWSV RDLVMWDNRS LMHRARRHFP DTDVREMHRA STIDDLTWVR PKTIALAG
NT Sequence
ATGAGCCTGG ATATCGAACC TGCCCATCCA ACAATCGCAG CGCGCATTCG CGGTCTGGAT CTGCGCCAGC CGCTCAGCGA CGAACAGGTC GATGAGATCG GCCAGGCAAG CGCCATTTAC CCTGTGCTGA TCTTCCCCAA TCAGTTGATC GACGACGCGC AGCTGATGGC GTTCAGTCAG AACTTCGGTC CGTTGCAGCC GGTGGTTTCG TTTCATACGG CGAAAGCCGA TCATCGGCTC TCGCCGATGG TGTCGGACAT CTCCAACCTC GACAAGAACA ACCGGACGTT CCCCGCCGGC GACCGGCGCA GGATGAATTT CCTGAGCAGC CGGCGCTGGC ACACGGACGG TTCCTATCTG CCCACGCCGA ACCGCTATTC GATGCTGCTC GCGTATACGG TCGCCCGCGT TGGCGGGCAG ACGCAGTTCG CCGACATGCG CGCGGCGTAC GACGCGCTGC CCGCCGAGTG GCTGGAACTC GTCGAAGATC TGACGCTGGA GCACAACGTG ATGCATTCGC GCGCGGTGGC CGGCTTCACG GATTTCGACG AAGAGGAACG GCGGCGCTTT CCGGCGACCC ATAAAAAACT GGTGCGGCGG CATCCGGTTT CCGGACGCCT GTCGCTGTAC CTTTCCGGGC ACGCATCGCA TGTCGTCGGC TGGCCCGTTC CGGAAGGACT CGATCTGCTG CGCGAGCTGA CCGAATTCGC CACGCAGCCG CAGTTCGTCT ACACGCACGA GTGGTCGGTC CGCGACCTCG TGATGTGGGA CAACCGTTCG CTGATGCATC GCGCGCGCCG CCATTTTCCG GATACCGACG TGCGCGAGAT GCATCGCGCC TCCACCATCG ACGACCTCAC GTGGGTCCGT CCAAAAACCA TTGCGCTGGC GGGA
Details for BuxeA.00024.d.B1.GE39344
HARVESTED ON: 7/10/2015
SEQUENCED ON: 7/6/2015
EXPECTED MW: 35kDa
OBSERVED MW: 35kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMS LDIEPAHPTI AARIRGLDLR QPLSDEQVDE IGQASAIYPV LIFPNQLIDD AQLMAFSQNF GPLQPVVSFH TAKADHRLSP MVSDISNLDK NNRTFPAGDR RRMNFLSSRR WHTDGSYLPT PNRYSMLLAY TVARVGGQTQ FADMRAAYDA LPAEWLELVE DLTLEHNVMH SRAVAGFTDF DEEERRRFPA THKKLVRRHP VSGRLSLYLS GHASHVVGWP VPEGLDLLRE LTEFATQPQF VYTHEWSVRD LVMWDNRSLM HRARRHFPDT DVREMHRAST IDDLTWVRPK TIALAG
Validated NT Sequence
tcacttatcc cgccagcgca atggtttttg gacggaccca cgtgaggtcg tcgatggtgg aggcgcgatg catctcgcgc acgtcggtat ccggaaaatg gcggcgcgcg cgatgcatca gcgaacggtt gtcccacatc acgaggtcgc ggaccgacca ctcgtgcgtg tagacgaact gcggctgcgt ggcgaattcg gtcagctcgc gcagcagatc gagtccttcc ggaacgggcc agccgacgac atgcgatgcg tgcccggaaa ggtacagcga caggcgtccg gaaaccggat gccgccgcac cagtttttta tgggtcgccg gaaagcgccg ccgttcctct tcgtcgaaat ccgtgaagcc ggccaccgcg cgcgaatgca tcacgttgtg ctccagcgtc agatcttcga cgagttccag ccactcggcg ggcagcgcgt cgtacgccgc gcgcatgtcg gcgaactgcg tctgcccgcc aacgcgggcg accgtatacg cgagcagcat cgaatagcgg ttcggcgtgg gcagatagga accgtccgtg tgccagcgcc ggctgctcag gaaattcatc ctgcgccggt cgccggcggg gaacgtccgg ttgttcttgt cgaggttgga gatgtccgac accatcggcg agagccgatg atcggctttc gccgtatgaa acgaaaccac cggctgcaac ggaccgaagt tctgactgaa cgccatcagc tgcgcgtcgt cgatcaactg attggggaag atcagcacag ggtaaatggc gcttgcctgg ccgatctcat cgacctgttc gtcgctgagc ggctggcgca gatccagacc gcgaatgcgc gctgcgattg ttggatgggc aggttcgata tccaggctca tatggtggtg gtggtggtga gccat
Expected Protein Sequence
MAHHHHHHMS LDIEPAHPTI AARIRGLDLR QPLSDEQVDE IGQASAIYPV LIFPNQLIDD AQLMAFSQNF GPLQPVVSFH TAKADHRLSP MVSDISNLDK NNRTFPAGDR RRMNFLSSRR WHTDGSYLPT PNRYSMLLAY TVARVGGQTQ FADMRAAYDA LPAEWLELVE DLTLEHNVMH SRAVAGFTDF DEEERRRFPA THKKLVRRHP VSGRLSLYLS GHASHVVGWP VPEGLDLLRE LTEFATQPQF VYTHEWSVRD LVMWDNRSLM HRARRHFPDT DVREMHRAST IDDLTWVRPK TIALAG
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgagcc tggatatcga acctgcccat ccaacaatcg cagcgcgcat tcgcggtctg gatctgcgcc agccgctcag cgacgaacag gtcgatgaga tcggccaggc aagcgccatt taccctgtgc tgatcttccc caatcagttg atcgacgacg cgcagctgat ggcgttcagt cagaacttcg gtccgttgca gccggtggtt tcgtttcata cggcgaaagc cgatcatcgg ctctcgccga tggtgtcgga catctccaac ctcgacaaga acaaccggac gttccccgcc ggcgaccggc gcaggatgaa tttcctgagc agccggcgct ggcacacgga cggttcctat ctgcccacgc cgaaccgcta ttcgatgctg ctcgcgtata cggtcgcccg cgttggcggg cagacgcagt tcgccgacat gcgcgcggcg tacgacgcgc tgcccgccga gtggctggaa ctcgtcgaag atctgacgct ggagcacaac gtgatgcatt cgcgcgcggt ggccggcttc acggatttcg acgaagagga acggcggcgc tttccggcga cccataaaaa actggtgcgg cggcatccgg tttccggacg cctgtcgctg tacctttccg ggcacgcatc gcatgtcgtc ggctggcccg ttccggaagg actcgatctg ctgcgcgagc tgaccgaatt cgccacgcag ccgcagttcg tctacacgca cgagtggtcg gtccgcgacc tcgtgatgtg ggacaaccgt tcgctgatgc atcgcgcgcg ccgccatttt ccggataccg acgtgcgcga gatgcatcgc gcctccacca tcgacgacct cacgtgggtc cgtccaaaaa ccattgcgct ggcgggatga gtaagatagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaattcttga agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgcagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatata tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag ctgcggtaaa gctcatcagc gtggtcgtga agcgattcac agatgtctgc ctgttcatcc gcgtccagct cgttgagttt ctccagaagc gttaatgtct ggcttctgat aaagcgggcc atgttaaggg cggttttttc ctgtttggtc actgatgcct ccgtgtaagg gggatttctg ttcatggggg taatgatacc gatgaaacga gagaggatgc tcacgatacg ggttactgat gatgaacatg cccggttact ggaacgttgt gagggtaaac aactggcggt atggatgcgg cgggaccaga gaaaaatcac tcagggtcaa tgccagcgct tcgttaatac agatgtaggt gttccacagg gtagccagca gcatcctgcg atgcagatcc ggaacataat ggtgcagggc gctgacttcc gcgtttccag actttacgaa acacggaaac cgaagaccat tcatgttgtt gctcaggtcg cagacgtttt gcagcagcag tcgcttcacg ttcgctcgcg tatcggtgat tcattctgct aaccagtaag gcaaccccgc cagcctagcc gggtcctcaa cgacaggagc acgatcatgc gcacccgtgg ccaggaccca acgctgcccg agatgcgccg cgtgcggctg ctggagatgg cggacgcgat ggatatgttc tgccaagggt tggtttgcgc attcacagtt ctccgcaaga attgattggc tccaattctt ggagtggtga atccgttagc gaggtgccgc cggcttccat tcaggtcgag gtggcccggc tccatgcacc gcgacgcaac gcggggaggc agacaaggta tagggcggcg cctacaatcc atgccaaccc gttccatgtg ctcgccgagg cggcataaat cgccgtgacg atcagcggtc cagtgatcga agttaggctg gtaagagccg cgagcgatcc ttgaagctgt ccctgatggt cgtcatctac ctgcctggac agcatggcct gcaacgcggg catcccgatg ccgccggaag cgagaagaat cataatgggg aaggccatcc agcctcgcgt cgcgaacgcc agcaagacgt agcccagcgc gtcggccgcc atgccggcga taatggcctg cttctcgccg aaacgtttgg tggcgggacc agtgacgaag gcttgagcga gggcgtgcaa gattccgaat accgcaagcg acaggccgat catcgtcgcg ctccagcgaa agcggtcctc gccgaaaatg acccagagcg ctgccggcac ctgtcctacg agttgcatga taaagaagac agtcataagt gcggcgacga tagtcatgcc ccgcgcccac cggaaggagc tgactgggtt gaaggctctc aagggcatcg gtcgacgctc tcccttatgc gactcctgca ttaggaagca gcccagtagt aggttgaggc cgttgagcac cgccgccgca aggaatggtg catgcaagga gatggcgccc aacagtcccc cggccacggg gcctgccacc atacccacgc cgaaacaagc gctcatgagc ccgaagtggc gagcccgatc ttccccatcg gtgatgtcgg cgatataggc gccagcaacc gcacctgtgg cgccggtgat gccggccacg atgcgtccgg cgtagaggat cgagatctcg atcccgcgaa at