SSGCID: Target Page

BuxeA.00013.a

Holliday junction ATP-dependent DNA helicase ruvB

CENTER ID:	BuxeA.00013.a
ORGANISM:	Burkholderia xenovorans LB400
ASSOCIATED DISEASE:
CURRENT STATUS:	crystallized
COMMUNITY REQUEST:	False
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BuxeA.00013.a.B1.GE39338	Full length( BuxeA.00013.a )		1	355

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BuxeA.00013.a.B1.PS02529	Full length( BuxeA.00013.a )		1	355

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|266265.5.peg.3998
RefSeq:	YP_560371.1
UniProt:	Q13UC0

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MIETDKLAAE RIIAATPVSP NEEAFERALR PRQLEEYVGQ EKVRGQLEIF IEAAKRRSES LDHVLLFGPP GLGKTTLAHI IAREMGVNLR QTSGPVLERA GDLAALLTNL EANDVLFIDE IHRLSPVVEE ILYPALEDYQ IDIMIGEGPA ARSVKLDLQP FTLVGATTRA GMLTNPLRDR FGIVARLEFY NAEQLARIVT RSASLLHAQI HPDGAFEIAK RARGTPRIAN RLLRRVRDFA EVKADGNITA QVADAALKML DVDAVGFDLM DRKLLEAILH KFDGGPVGVD NLAAAIGEER DTIEDVLEPY LIQQGFLQRT PRGRVATLLT YRHFGLAAPD SSSSTLPGLW DSAAT

NT Sequence

ATGATCGAAA CCGACAAACT CGCCGCCGAG CGCATCATCG CGGCCACGCC CGTCTCGCCG AACGAAGAAG CGTTCGAGCG TGCGTTGCGC CCGCGCCAGC TCGAAGAATA TGTCGGGCAG GAAAAAGTGC GCGGCCAGCT GGAAATCTTT ATCGAGGCCG CCAAACGCCG CTCCGAATCG CTTGACCACG TCTTGCTGTT CGGCCCGCCG GGCCTCGGCA AAACCACGCT CGCGCACATC ATCGCGCGGG AAATGGGTGT CAATCTCCGG CAAACGTCCG GGCCGGTGCT GGAACGCGCC GGCGACCTCG CCGCGCTGCT CACCAATCTC GAAGCGAACG ACGTGCTGTT CATCGACGAA ATCCACCGGC TCTCGCCGGT CGTCGAAGAA ATTCTGTACC CGGCGCTGGA GGATTATCAG ATCGACATCA TGATCGGCGA AGGACCGGCC GCGCGCAGCG TGAAGCTGGA CCTGCAGCCG TTCACGCTGG TCGGCGCGAC CACGCGCGCG GGCATGCTGA CCAACCCGCT GCGCGACCGC TTCGGAATCG TGGCGCGGCT CGAGTTTTAT AACGCCGAGC AACTGGCGCG CATCGTCACG CGTTCGGCGT CGCTGCTCCA TGCGCAGATC CATCCGGACG GCGCGTTCGA AATCGCCAAA CGCGCGCGCG GCACGCCGCG GATCGCGAAC CGCCTGCTGC GGCGCGTGCG CGACTTCGCC GAGGTGAAGG CCGACGGCAA TATCACCGCG CAAGTGGCCG ACGCCGCGCT CAAGATGCTC GACGTGGACG CGGTCGGCTT CGACCTGATG GACCGCAAGC TGCTCGAAGC GATTTTGCAC AAGTTCGACG GCGGCCCGGT CGGCGTCGAC AACCTGGCGG CGGCTATCGG CGAAGAGCGC GACACGATTG AAGACGTGCT CGAACCGTAT CTGATCCAGC AGGGCTTCCT GCAGCGCACG CCGCGCGGCC GCGTCGCCAC GCTGCTTACG TACCGGCATT TCGGCCTCGC CGCGCCCGAC TCGTCGAGCA GCACGCTGCC CGGCCTGTGG GATTCGGCCG CCACC

Details for BuxeA.00013.a.B1.GE39338

HARVESTED ON:	7/10/2015
SEQUENCED ON:	7/6/2015
EXPECTED MW:	40kDa
OBSERVED MW:	40kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY:	data unavailable
PERCENT COVERAGE:	data unavailable

Validated AA Sequence

sequence unavailable

Validated NT Sequence

sequence unavailable

Expected Protein Sequence

MAHHHHHHMI ETDKLAAERI IAATPVSPNE EAFERALRPR QLEEYVGQEK VRGQLEIFIE AAKRRSESLD HVLLFGPPGL GKTTLAHIIA REMGVNLRQT SGPVLERAGD LAALLTNLEA NDVLFIDEIH RLSPVVEEIL YPALEDYQID IMIGEGPAAR SVKLDLQPFT LVGATTRAGM LTNPLRDRFG IVARLEFYNA EQLARIVTRS ASLLHAQIHP DGAFEIAKRA RGTPRIANRL LRRVRDFAEV KADGNITAQV ADAALKMLDV DAVGFDLMDR KLLEAILHKF DGGPVGVDNL AAAIGEERDT IEDVLEPYLI QQGFLQRTPR GRVATLLTYR HFGLAAPDSS SSTLPGLWDS AAT

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgatcg aaaccgacaa actcgccgcc gagcgcatca tcgcggccac gcccgtctcg ccgaacgaag aagcgttcga gcgtgcgttg cgcccgcgcc agctcgaaga atatgtcggg caggaaaaag tgcgcggcca gctggaaatc tttatcgagg ccgccaaacg ccgctccgaa tcgcttgacc acgtcttgct gttcggcccg ccgggcctcg gcaaaaccac gctcgcgcac atcatcgcgc gggaaatggg tgtcaatctc cggcaaacgt ccgggccggt gctggaacgc gccggcgacc tcgccgcgct gctcaccaat ctcgaagcga acgacgtgct gttcatcgac gaaatccacc ggctctcgcc ggtcgtcgaa gaaattctgt acccggcgct ggaggattat cagatcgaca tcatgatcgg cgaaggaccg gccgcgcgca gcgtgaagct ggacctgcag ccgttcacgc tggtcggcgc gaccacgcgc gcgggcatgc tgaccaaccc gctgcgcgac cgcttcggaa tcgtggcgcg gctcgagttt tataacgccg agcaactggc gcgcatcgtc acgcgttcgg cgtcgctgct ccatgcgcag atccatccgg acggcgcgtt cgaaatcgcc aaacgcgcgc gcggcacgcc gcggatcgcg aaccgcctgc tgcggcgcgt gcgcgacttc gccgaggtga aggccgacgg caatatcacc gcgcaagtgg ccgacgccgc gctcaagatg ctcgacgtgg acgcggtcgg cttcgacctg atggaccgca agctgctcga agcgattttg cacaagttcg acggcggccc ggtcggcgtc gacaacctgg cggcggctat cggcgaagag cgcgacacga ttgaagacgt gctcgaaccg tatctgatcc agcagggctt cctgcagcgc acgccgcgcg gccgcgtcgc cacgctgctt acgtaccggc atttcggcct cgccgcgccc gactcgtcga gcagcacgct gcccggcctg tgggattcgg ccgccacctg agtaagatag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaattcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgcagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gctgcggtaa agctcatcag cgtggtcgtg aagcgattca cagatgtctg cctgttcatc cgcgtccagc tcgttgagtt tctccagaag cgttaatgtc tggcttctga taaagcgggc catgttaagg gcggtttttt cctgtttggt cactgatgcc tccgtgtaag ggggatttct gttcatgggg gtaatgatac cgatgaaacg agagaggatg ctcacgatac gggttactga tgatgaacat gcccggttac tggaacgttg tgagggtaaa caactggcgg tatggatgcg gcgggaccag agaaaaatca ctcagggtca atgccagcgc ttcgttaata cagatgtagg tgttccacag ggtagccagc agcatcctgc gatgcagatc cggaacataa tggtgcaggg cgctgacttc cgcgtttcca gactttacga aacacggaaa ccgaagacca ttcatgttgt tgctcaggtc gcagacgttt tgcagcagca gtcgcttcac gttcgctcgc gtatcggtga ttcattctgc taaccagtaa ggcaaccccg ccagcctagc cgggtcctca acgacaggag cacgatcatg cgcacccgtg gccaggaccc aacgctgccc gagatgcgcc gcgtgcggct gctggagatg gcggacgcga tggatatgtt ctgccaaggg ttggtttgcg cattcacagt tctccgcaag aattgattgg ctccaattct tggagtggtg aatccgttag cgaggtgccg ccggcttcca ttcaggtcga ggtggcccgg ctccatgcac cgcgacgcaa cgcggggagg cagacaaggt atagggcggc gcctacaatc catgccaacc cgttccatgt gctcgccgag gcggcataaa tcgccgtgac gatcagcggt ccagtgatcg aagttaggct ggtaagagcc gcgagcgatc cttgaagctg tccctgatgg tcgtcatcta cctgcctgga cagcatggcc tgcaacgcgg gcatcccgat gccgccggaa gcgagaagaa tcataatggg gaaggccatc cagcctcgcg tcgcgaacgc cagcaagacg tagcccagcg cgtcggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga aat

Details for BuxeA.00013.a.B1.PS02529

PURIFICATION DATe:	11/3/2015
CONCENTRATION:	18.58mg/ml
OBSERVED MW:	39kDa
EXPRESSION LEVEL:	Moderate Expression
PROTEIN PURIFICATION BUFFER:	SEC buffer
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	8
VIAL VOLUME:	200µl
PERCENT IDENTITY:	data unavailable
PERCENT COVERAGE:	data unavailable

Protocol Notes

notes unavailable

Validated AA Sequence

sequence unavailable

Validated NT Sequence

sequence unavailable

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGATCG AAACCGACAA ACTCGCCGCC GAGCGCATCA TCGCGGCCAC GCCCGTCTCG CCGAACGAAG AAGCGTTCGA GCGTGCGTTG CGCCCGCGCC AGCTCGAAGA ATATGTCGGG CAGGAAAAAG TGCGCGGCCA GCTGGAAATC TTTATCGAGG CCGCCAAACG CCGCTCCGAA TCGCTTGACC ACGTCTTGCT GTTCGGCCCG CCGGGCCTCG GCAAAACCAC GCTCGCGCAC ATCATCGCGC GGGAAATGGG TGTCAATCTC CGGCAAACGT CCGGGCCGGT GCTGGAACGC GCCGGCGACC TCGCCGCGCT GCTCACCAAT CTCGAAGCGA ACGACGTGCT GTTCATCGAC GAAATCCACC GGCTCTCGCC GGTCGTCGAA GAAATTCTGT ACCCGGCGCT GGAGGATTAT CAGATCGACA TCATGATCGG CGAAGGACCG GCCGCGCGCA GCGTGAAGCT GGACCTGCAG CCGTTCACGC TGGTCGGCGC GACCACGCGC GCGGGCATGC TGACCAACCC GCTGCGCGAC CGCTTCGGAA TCGTGGCGCG GCTCGAGTTT TATAACGCCG AGCAACTGGC GCGCATCGTC ACGCGTTCGG CGTCGCTGCT CCATGCGCAG ATCCATCCGG ACGGCGCGTT CGAAATCGCC AAACGCGCGC GCGGCACGCC GCGGATCGCG AACCGCCTGC TGCGGCGCGT GCGCGACTTC GCCGAGGTGA AGGCCGACGG CAATATCACC GCGCAAGTGG CCGACGCCGC GCTCAAGATG CTCGACGTGG ACGCGGTCGG CTTCGACCTG ATGGACCGCA AGCTGCTCGA AGCGATTTTG CACAAGTTCG ACGGCGGCCC GGTCGGCGTC GACAACCTGG CGGCGGCTAT CGGCGAAGAG CGCGACACGA TTGAAGACGT GCTCGAACCG TATCTGATCC AGCAGGGCTT CCTGCAGCGC ACGCCGCGCG GCCGCGTCGC CACGCTGCTT ACGTACCGGC ATTTCGGCCT CGCCGCGCCC GACTCGTCGA GCAGCACGCT GCCCGGCCTG TGGGATTCGG CCGCCACCTG AGTAAGATAG GATCCGGCTG CTAACAAAGC CCGAAAGGAA GCTGAGTTGG CTGCTGCCAC CGCTGAGCAA TAACTAGCAT AACCCCTTGG GGCCTCTAAA CGGGTCTTGA GGGGTTTTTT GCTGAAAGGA GGAACTATAT CCGGATATCC ACAGGACGGG TGTGGTCGCC ATGATCGCGT AGTCGATAGT GGCTCCAAGT AGCGAAGCGA GCAGGACTGG GCGGCGGCCA AAGCGGTCGG ACAGTGCTCC GAGAACGGGT GCGCATAGAA ATTGCATCAA CGCATATAGC GCTAGCAGCA CGCCATAGTG ACTGGCGATG CTGTCGGAAT GGACGATATC CCGCAAGAGG CCCGGCAGTA CCGGCATAAC CAAGCCTATG CCTACAGCAT CCAGGGTGAC GGTGCCGAGG ATGACGATGA GCGCATTGTT AGATTTCATA CACGGTGCCT GACTGCGTTA GCAATTTAAC TGTGATAAAC TACCGCATTA AAGCTTATCG ATGATAAGCT GTCAAACATG AGAATTCTTG AAGACGAAAG GGCCTCGTGA TACGCCTATT TTTATAGGTT AATGTCATGA TAATAATGGT TTCTTAGACG TCAGGTGGCA CTTTTCGGGG AAATGTGCGC GGAACCCCTA TTTGTTTATT TTTCTAAATA CATTCAAATA TGTATCCGCT CATGAGACAA TAACCCTGAT AAATGCTTCA ATAATATTGA AAAAGGAAGA GTATGAGTAT TCAACATTTC CGTGTCGCCC TTATTCCCTT TTTTGCGGCA TTTTGCCTTC CTGTTTTTGC TCACCCAGAA ACGCTGGTGA AAGTAAAAGA TGCTGAAGAT CAGTTGGGTG CACGAGTGGG TTACATCGAA CTGGATCTCA ACAGCGGTAA GATCCTTGAG AGTTTTCGCC CCGAAGAACG TTTTCCAATG ATGAGCACTT TTAAAGTTCT GCTATGTGGC GCGGTATTAT CCCGTGTTGA CGCCGGGCAA GAGCAACTCG GTCGCCGCAT ACACTATTCT CAGAATGACT TGGTTGAGTA CTCACCAGTC ACAGAAAAGC ATCTTACGGA TGGCATGACA GTAAGAGAAT TATGCAGTGC TGCCATAACC ATGAGTGATA ACACTGCGGC CAACTTACTT CTGACAACGA TCGGAGGACC GAAGGAGCTA ACCGCTTTTT TGCACAACAT GGGGGATCAT GTAACTCGCC TTGATCGTTG GGAACCGGAG CTGAATGAAG CCATACCAAA CGACGAGCGT GACACCACGA TGCCTGCAGC AATGGCAACA ACGTTGCGCA AACTATTAAC TGGCGAACTA CTTACTCTAG CTTCCCGGCA ACAATTAATA GACTGGATGG AGGCGGATAA AGTTGCAGGA CCACTTCTGC GCTCGGCCCT TCCGGCTGGC TGGTTTATTG CTGATAAATC TGGAGCCGGT GAGCGTGGGT CTCGCGGTAT CATTGCAGCA CTGGGGCCAG ATGGTAAGCC CTCCCGTATC GTAGTTATCT ACACGACGGG GAGTCAGGCA ACTATGGATG AACGAAATAG ACAGATCGCT GAGATAGGTG CCTCACTGAT TAAGCATTGG TAACTGTCAG ACCAAGTTTA CTCATATATA CTTTAGATTG ATTTAAAACT TCATTTTTAA TTTAAAAGGA TCTAGGTGAA GATCCTTTTT GATAATCTCA TGACCAAAAT CCCTTAACGT GAGTTTTCGT TCCACTGAGC GTCAGACCCC GTAGAAAAGA TCAAAGGATC TTCTTGAGAT CCTTTTTTTC TGCGCGTAAT CTGCTGCTTG CAAACAAAAA AACCACCGCT ACCAGCGGTG GTTTGTTTGC CGGATCAAGA GCTACCAACT CTTTTTCCGA AGGTAACTGG CTTCAGCAGA GCGCAGATAC CAAATACTGT CCTTCTAGTG TAGCCGTAGT TAGGCCACCA CTTCAAGAAC TCTGTAGCAC CGCCTACATA CCTCGCTCTG CTAATCCTGT TACCAGTGGC TGCTGCCAGT GGCGATAAGT CGTGTCTTAC CGGGTTGGAC TCAAGACGAT AGTTACCGGA TAAGGCGCAG CGGTCGGGCT GAACGGGGGG TTCGTGCACA CAGCCCAGCT TGGAGCGAAC GACCTACACC GAACTGAGAT ACCTACAGCG TGAGCTATGA GAAAGCGCCA CGCTTCCCGA AGGGAGAAAG GCGGACAGGT ATCCGGTAAG CGGCAGGGTC GGAACAGGAG AGCGCACGAG GGAGCTTCCA GGGGGAAACG CCTGGTATCT TTATAGTCCT GTCGGGTTTC GCCACCTCTG ACTTGAGCGT CGATTTTTGT GATGCTCGTC AGGGGGGCGG AGCCTATGGA AAAACGCCAG CAACGCGGCC TTTTTACGGT TCCTGGCCTT TTGCTGGCCT TTTGCTCACA TGTTCTTTCC TGCGTTATCC CCTGATTCTG TGGATAACCG TATTACCGCC TTTGAGTGAG CTGATACCGC TCGCCGCAGC CGAACGACCG AGCGCAGCGA GTCAGTGAGC GAGGAAGCGG AAGAGCGCCT GATGCGGTAT TTTCTCCTTA CGCATCTGTG CGGTATTTCA CACCGCATAT ATGGTGCACT CTCAGTACAA TCTGCTCTGA TGCCGCATAG TTAAGCCAGT ATACACTCCG CTATCGCTAC GTGACTGGGT CATGGCTGCG CCCCGACACC CGCCAACACC CGCTGACGCG CCCTGACGGG CTTGTCTGCT CCCGGCATCC GCTTACAGAC AAGCTGTGAC CGTCTCCGGG AGCTGCATGT GTCAGAGGTT TTCACCGTCA TCACCGAAAC GCGCGAGGCA GCTGCGGTAA AGCTCATCAG CGTGGTCGTG AAGCGATTCA CAGATGTCTG CCTGTTCATC CGCGTCCAGC TCGTTGAGTT TCTCCAGAAG CGTTAATGTC TGGCTTCTGA TAAAGCGGGC CATGTTAAGG GCGGTTTTTT CCTGTTTGGT CACTGATGCC TCCGTGTAAG GGGGATTTCT GTTCATGGGG GTAATGATAC CGATGAAACG AGAGAGGATG CTCACGATAC GGGTTACTGA TGATGAACAT GCCCGGTTAC TGGAACGTTG TGAGGGTAAA CAACTGGCGG TATGGATGCG GCGGGACCAG AGAAAAATCA CTCAGGGTCA ATGCCAGCGC TTCGTTAATA CAGATGTAGG TGTTCCACAG GGTAGCCAGC AGCATCCTGC GATGCAGATC CGGAACATAA TGGTGCAGGG CGCTGACTTC CGCGTTTCCA GACTTTACGA AACACGGAAA CCGAAGACCA TTCATGTTGT TGCTCAGGTC GCAGACGTTT TGCAGCAGCA GTCGCTTCAC GTTCGCTCGC GTATCGGTGA TTCATTCTGC TAACCAGTAA GGCAACCCCG CCAGCCTAGC CGGGTCCTCA ACGACAGGAG CACGATCATG CGCACCCGTG GCCAGGACCC AACGCTGCCC GAGATGCGCC GCGTGCGGCT GCTGGAGATG GCGGACGCGA TGGATATGTT CTGCCAAGGG TTGGTTTGCG CATTCACAGT TCTCCGCAAG AATTGATTGG CTCCAATTCT TGGAGTGGTG AATCCGTTAG CGAGGTGCCG CCGGCTTCCA TTCAGGTCGA GGTGGCCCGG CTCCATGCAC CGCGACGCAA CGCGGGGAGG CAGACAAGGT ATAGGGCGGC GCCTACAATC CATGCCAACC CGTTCCATGT GCTCGCCGAG GCGGCATAAA TCGCCGTGAC GATCAGCGGT CCAGTGATCG AAGTTAGGCT GGTAAGAGCC GCGAGCGATC CTTGAAGCTG TCCCTGATGG TCGTCATCTA CCTGCCTGGA CAGCATGGCC TGCAACGCGG GCATCCCGAT GCCGCCGGAA GCGAGAAGAA TCATAATGGG GAAGGCCATC CAGCCTCGCG TCGCGAACGC CAGCAAGACG TAGCCCAGCG CGTCGGCCGC CATGCCGGCG ATAATGGCCT GCTTCTCGCC GAAACGTTTG GTGGCGGGAC CAGTGACGAA GGCTTGAGCG AGGGCGTGCA AGATTCCGAA TACCGCAAGC GACAGGCCGA TCATCGTCGC GCTCCAGCGA AAGCGGTCCT CGCCGAAAAT GACCCAGAGC GCTGCCGGCA CCTGTCCTAC GAGTTGCATG ATAAAGAAGA CAGTCATAAG TGCGGCGACG ATAGTCATGC CCCGCGCCCA CCGGAAGGAG CTGACTGGGT TGAAGGCTCT CAAGGGCATC GGTCGACGCT CTCCCTTATG CGACTCCTGC ATTAGGAAGC AGCCCAGTAG TAGGTTGAGG CCGTTGAGCA CCGCCGCCGC AAGGAATGGT GCATGCAAGG AGATGGCGCC CAACAGTCCC CCGGCCACGG GGCCTGCCAC CATACCCACG CCGAAACAAG CGCTCATGAG CCCGAAGTGG CGAGCCCGAT CTTCCCCATC GGTGATGTCG GCGATATAGG CGCCAGCAAC CGCACCTGTG GCGCCGGTGA TGCCGGCCAC GATGCGTCCG GCGTAGAGGA TCGAGATCTC GATCCCGCGA AAT