SSGCID: Target Page

BuxeA.00010.x

Putative short-chain dehydrogenase/reductase

CENTER ID:	BuxeA.00010.x
ORGANISM:	Burkholderia xenovorans LB400
ASSOCIATED DISEASE:
CURRENT STATUS:	cloned
COMMUNITY REQUEST:	False
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BuxeA.00010.x.B1.GE39424	Full length( BuxeA.00010.x )		1	256

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|266265.5.peg.8077
RefSeq:	YP_555570.1
UniProt:	Q13I69

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MNELTDTLIA ITGGGSGLGE ACAELLAPRV AELALIDRDP DSLASVRARL SGLCEISVHA CDIANSDDVS TTFNRIERES RVVSTLINCA GMNVKDRHFG ELTPDGWNQV IAVNLSGMYY CCQAVLSGMR RQQRGTIINV SSWAGRHAGF FTGPAYNASK RGVLALTESI NLEEGINNVR ATAVVPEAMA TPMIRKIPNP PSEEAIAKMM RPLDVARMID LIISLPQEMC VNEVVMSPTW NLSYLGAYET AIDRMV

NT Sequence

ATGAACGAAC TTACAGATAC CTTGATTGCC ATTACAGGTG GTGGTTCTGG ACTTGGCGAA GCATGCGCTG AACTGCTGGC CCCTCGGGTA GCCGAACTTG CGTTGATAGA TCGTGACCCG GACTCGCTTG CCAGTGTGCG GGCGCGGCTG TCGGGATTAT GCGAGATATC CGTTCATGCT TGCGACATTG CTAACAGCGA TGACGTCTCC ACAACATTCA ATCGGATCGA GCGAGAGTCT CGGGTAGTGA GCACACTTAT TAACTGTGCA GGAATGAACG TGAAAGATCG ACACTTCGGT GAGCTTACTC CCGACGGATG GAATCAGGTC ATTGCAGTTA ATCTGTCCGG GATGTACTAC TGCTGTCAGG CGGTATTGAG TGGTATGCGG CGTCAGCAGC GCGGGACAAT TATCAACGTG TCGTCTTGGG CCGGTCGACA CGCGGGATTC TTCACTGGAC CCGCTTACAA CGCCAGCAAG AGGGGTGTCC TGGCTTTGAC GGAGTCAATT AACCTGGAAG AGGGAATAAA CAACGTCCGG GCGACAGCAG TCGTACCTGA AGCAATGGCG ACCCCGATGA TCCGGAAAAT TCCGAATCCA CCAAGCGAAG AGGCGATTGC GAAAATGATG CGACCTCTCG ACGTGGCGAG GATGATCGAC CTGATAATCA GTTTGCCGCA GGAAATGTGT GTAAACGAGG TTGTCATGAG CCCGACATGG AATCTGTCCT ATCTCGGAGC CTACGAGACG GCAATCGACA GGATGGTT

Details for BuxeA.00010.x.B1.GE39424

HARVESTED ON:	data unavailable
SEQUENCED ON:	8/13/2015
EXPECTED MW:	29kDa
OBSERVED MW:	40kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Insoluble
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY:	data unavailable
PERCENT COVERAGE:	data unavailable

Validated AA Sequence

sequence unavailable

Validated NT Sequence

sequence unavailable

Expected Protein Sequence

MAHHHHHHMN ELTDTLIAIT GGGSGLGEAC AELLAPRVAE LALIDRDPDS LASVRARLSG LCEISVHACD IANSDDVSTT FNRIERESRV VSTLINCAGM NVKDRHFGEL TPDGWNQVIA VNLSGMYYCC QAVLSGMRRQ QRGTIINVSS WAGRHAGFFT GPAYNASKRG VLALTESINL EEGINNVRAT AVVPEAMATP MIRKIPNPPS EEAIAKMMRP LDVARMIDLI ISLPQEMCVN EVVMSPTWNL SYLGAYETAI DRMV

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgaacg aacttacaga taccttgatt gccattacag gtggtggttc tggacttggc gaagcatgcg ctgaactgct ggcccctcgg gtagccgaac ttgcgttgat agatcgtgac ccggactcgc ttgccagtgt gcgggcgcgg ctgtcgggat tatgcgagat atccgttcat gcttgcgaca ttgctaacag cgatgacgtc tccacaacat tcaatcggat cgagcgagag tctcgggtag tgagcacact tattaactgt gcaggaatga acgtgaaaga tcgacacttc ggtgagctta ctcccgacgg atggaatcag gtcattgcag ttaatctgtc cgggatgtac tactgctgtc aggcggtatt gagtggtatg cggcgtcagc agcgcgggac aattatcaac gtgtcgtctt gggccggtcg acacgcggga ttcttcactg gacccgctta caacgccagc aagaggggtg tcctggcttt gacggagtca attaacctgg aagagggaat aaacaacgtc cgggcgacag cagtcgtacc tgaagcaatg gcgaccccga tgatccggaa aattccgaat ccaccaagcg aagaggcgat tgcgaaaatg atgcgacctc tcgacgtggc gaggatgatc gacctgataa tcagtttgcc gcaggaaatg tgtgtaaacg aggttgtcat gagcccgaca tggaatctgt cctatctcgg agcctacgag acggcaatcg acaggatggt ttgagtaaga taggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaattc ttgaagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgc agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggccagga cccaacgctg cccgagatgc gccgcgtgcg gctgctggag atggcggacg cgatggatat gttctgccaa gggttggttt gcgcattcac agttctccgc aagaattgat tggctccaat tcttggagtg gtgaatccgt tagcgaggtg ccgccggctt ccattcaggt cgaggtggcc cggctccatg caccgcgacg caacgcgggg aggcagacaa ggtatagggc ggcgcctaca atccatgcca acccgttcca tgtgctcgcc gaggcggcat aaatcgccgt gacgatcagc ggtccagtga tcgaagttag gctggtaaga gccgcgagcg atccttgaag ctgtccctga tggtcgtcat ctacctgcct ggacagcatg gcctgcaacg cgggcatccc gatgccgccg gaagcgagaa gaatcataat ggggaaggcc atccagcctc gcgtcgcgaa cgccagcaag acgtagccca gcgcgtcggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaat