BuxeA.00010.v

Putative short-chain dehydrogenase

CENTER ID: BuxeA.00010.v
ORGANISM: Burkholderia xenovorans LB400
ASSOCIATED DISEASE:
CURRENT STATUS: expressed
COMMUNITY REQUEST: False
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BuxeA.00010.v.B2.GE39423 Mature protein( BuxeA.00010.v ) 27 246
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|266265.5.peg.8130
RefSeq: YP_555618.1
UniProt: Q13I21

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MMSLKGKIAM VTGAATGIGA TLAQALASSG AEVIGVDIAW DGAQQAGCVE RAQCDVADPM SVKTCVANIE AAHGPVDILI NNAALASELA PTPFEHIAPE EWLRVMTINT MAPFLCSQAV APRMRERKWG RIVNLTSATI FIGMPPMLHY VASKGAVAIM TRGLARELGR DGITVNAIAP GLTMTEGVQR NEAYSDELIA QAIAAQSIPN REQPEDLIAA CLYLVSDGAK MMTGQILAVD GGTAFH
NT Sequence
ATGATGAGTC TCAAGGGAAA GATTGCGATG GTAACCGGTG CGGCGACCGG CATCGGCGCT ACTCTGGCGC AAGCGCTGGC GTCGAGCGGT GCGGAAGTCA TCGGCGTCGA CATCGCATGG GATGGCGCTC AGCAGGCAGG CTGCGTCGAA CGAGCTCAGT GCGATGTCGC TGACCCAATG AGCGTCAAGA CTTGTGTTGC CAACATTGAA GCTGCCCACG GGCCAGTCGA TATCCTCATC AATAATGCGG CGCTGGCTTC CGAGCTGGCG CCGACGCCGT TCGAGCACAT CGCGCCCGAG GAATGGCTGC GGGTAATGAC AATCAACACG ATGGCGCCGT TCCTATGCAG TCAGGCGGTC GCTCCGCGCA TGCGTGAACG CAAATGGGGG CGTATCGTTA ATCTCACGTC GGCGACGATT TTTATCGGCA TGCCGCCTAT GTTGCACTAC GTCGCCAGCA AGGGGGCAGT CGCAATTATG ACCCGGGGGC TTGCGCGCGA ACTTGGACGC GACGGGATCA CCGTAAACGC GATCGCGCCC GGCCTCACCA TGACCGAAGG CGTTCAAAGG AATGAAGCCT ATTCCGATGA ACTGATAGCC CAGGCCATCG CCGCACAGAG CATCCCCAAT CGCGAGCAAC CCGAAGATCT GATTGCCGCC TGCCTTTATC TGGTCAGCGA CGGCGCGAAG ATGATGACGG GTCAGATTCT CGCTGTAGAT GGCGGTACGG CATTCCAT
Details for BuxeA.00010.v.B2.GE39423
HARVESTED ON: 8/5/2015
SEQUENCED ON: 8/13/2015
EXPECTED MW: 24kDa
OBSERVED MW: 24kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMA SSGAEVIGVD IAWDGAQQAG CVERAQCDVA DPMSVKTCVA NIEAAHGPVD ILINNAALAS ELAPTPFEHI APEEWLRVMT INTMAPFLCS QAVAPRMRER KWGRIVNLTS ATIFIGMPPM LHYVASKGAV AIMTRGLARE LGRDGITVNA IAPGLTMTEG VQRNEAYSDE LIAQAIAAQS IPNREQPEDL IAACLYLVSD GAKMMTGQIL AVDGGTAFH
Validated NT Sequence
atcttactca cttaatggaa tgccgtaccg ccatctacag cgagaatctg acccgtcatc atcttcgcgc cgtcgctgac cagataaagg caggcggcaa tcagatcttc gggttgctcg cgattgggga tgctctgtgc ggcgatggcc tgggctatca gttcatcgga ataggcttca ttcctttgaa cgccttcggt catggtgagg ccgggcgcga tcgcgtttac ggtgatcccg tcgcgtccaa gttcgcgcgc aagcccccgg gtcataattg cgactgcccc cttgctggcg acgtagtgca acataggcgg catgccgata aaaatcgtcg ccgacgtgag attaacgata cgcccccatt tgcgttcacg catgcgcgga gcgaccgcct gactgcatag gaacggcgcc atcgtgttga ttgtcattac ccgcagccat tcctcgggcg cgatgtgctc gaacggcgtc ggcgccagct cggaagccag cgccgcatta ttgatgagga tatcgactgg cccgtgggca gcttcaatgt tggcaacaca agtcttgacg ctcattgggt cagcgacatc gcactgagct cgttcgacgc agcctgcctg ctgagcgcca tcccatgcga tgtcgacgcc gatgacttcc gcaccgctcg acgccatatg gtggtggtgg tggtgagcca t
Expected Protein Sequence
MAHHHHHHAS SGAEVIGVDI AWDGAQQAGC VERAQCDVAD PMSVKTCVAN IEAAHGPVDI LINNAALASE LAPTPFEHIA PEEWLRVMTI NTMAPFLCSQ AVAPRMRERK WGRIVNLTSA TIFIGMPPML HYVASKGAVA IMTRGLAREL GRDGITVNAI APGLTMTEGV QRNEAYSDEL IAQAIAAQSI PNREQPEDLI AACLYLVSDG AKMMTGQILA VDGGTAFH
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgcgtcga gcggtgcgga agtcatcggc gtcgacatcg catgggatgg cgctcagcag gcaggctgcg tcgaacgagc tcagtgcgat gtcgctgacc caatgagcgt caagacttgt gttgccaaca ttgaagctgc ccacgggcca gtcgatatcc tcatcaataa tgcggcgctg gcttccgagc tggcgccgac gccgttcgag cacatcgcgc ccgaggaatg gctgcgggta atgacaatca acacgatggc gccgttccta tgcagtcagg cggtcgctcc gcgcatgcgt gaacgcaaat gggggcgtat cgttaatctc acgtcggcga cgatttttat cggcatgccg cctatgttgc actacgtcgc cagcaagggg gcagtcgcaa ttatgacccg ggggcttgcg cgcgaacttg gacgcgacgg gatcaccgta aacgcgatcg cgcccggcct caccatgacc gaaggcgttc aaaggaatga agcctattcc gatgaactga tagcccaggc catcgccgca cagagcatcc ccaatcgcga gcaacccgaa gatctgattg ccgcctgcct ttatctggtc agcgacggcg cgaagatgat gacgggtcag attctcgctg tagatggcgg tacggcattc cattgagtaa gataggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaat tcttgaagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gcagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagctgc ggtaaagctc atcagcgtgg tcgtgaagcg attcacagat gtctgcctgt tcatccgcgt ccagctcgtt gagtttctcc agaagcgtta atgtctggct tctgataaag cgggccatgt taagggcggt tttttcctgt ttggtcactg atgcctccgt gtaaggggga tttctgttca tgggggtaat gataccgatg aaacgagaga ggatgctcac gatacgggtt actgatgatg aacatgcccg gttactggaa cgttgtgagg gtaaacaact ggcggtatgg atgcggcggg accagagaaa aatcactcag ggtcaatgcc agcgcttcgt taatacagat gtaggtgttc cacagggtag ccagcagcat cctgcgatgc agatccggaa cataatggtg cagggcgctg acttccgcgt ttccagactt tacgaaacac ggaaaccgaa gaccattcat gttgttgctc aggtcgcaga cgttttgcag cagcagtcgc ttcacgttcg ctcgcgtatc ggtgattcat tctgctaacc agtaaggcaa ccccgccagc ctagccgggt cctcaacgac aggagcacga tcatgcgcac ccgtggccag gacccaacgc tgcccgagat gcgccgcgtg cggctgctgg agatggcgga cgcgatggat atgttctgcc aagggttggt ttgcgcattc acagttctcc gcaagaattg attggctcca attcttggag tggtgaatcc gttagcgagg tgccgccggc ttccattcag gtcgaggtgg cccggctcca tgcaccgcga cgcaacgcgg ggaggcagac aaggtatagg gcggcgccta caatccatgc caacccgttc catgtgctcg ccgaggcggc ataaatcgcc gtgacgatca gcggtccagt gatcgaagtt aggctggtaa gagccgcgag cgatccttga agctgtccct gatggtcgtc atctacctgc ctggacagca tggcctgcaa cgcgggcatc ccgatgccgc cggaagcgag aagaatcata atggggaagg ccatccagcc tcgcgtcgcg aacgccagca agacgtagcc cagcgcgtcg gccgccatgc cggcgataat ggcctgcttc tcgccgaaac gtttggtggc gggaccagtg acgaaggctt gagcgagggc gtgcaagatt ccgaataccg caagcgacag gccgatcatc gtcgcgctcc agcgaaagcg gtcctcgccg aaaatgaccc agagcgctgc cggcacctgt cctacgagtt gcatgataaa gaagacagtc ataagtgcgg cgacgatagt catgccccgc gcccaccgga aggagctgac tgggttgaag gctctcaagg gcatcggtcg acgctctccc ttatgcgact cctgcattag gaagcagccc agtagtaggt tgaggccgtt gagcaccgcc gccgcaagga atggtgcatg caaggagatg gcgcccaaca gtcccccggc cacggggcct gccaccatac ccacgccgaa acaagcgctc atgagcccga agtggcgagc ccgatcttcc ccatcggtga tgtcggcgat ataggcgcca gcaaccgcac ctgtggcgcc ggtgatgccg gccacgatgc gtccggcgta gaggatcgag atctcgatcc cgcgaaat