BuxeA.00010.u

Putative short-chain dehydrogenase/reductase

CENTER ID: BuxeA.00010.u
ORGANISM: Burkholderia xenovorans LB400
ASSOCIATED DISEASE:
CURRENT STATUS: expressed
COMMUNITY REQUEST: False
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BuxeA.00010.u.B2.GE39422 Mature protein( BuxeA.00010.u ) 29 231
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|266265.5.peg.8342
RefSeq: YP_555831.1
UniProt: Q13HF8

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MDTRQLAHRT IIVTGGLGAL GRSVGAVLAA RGARVVLIDR AEPTAVAGVA AVVGKVDLGE RESTAHAFAD VARQLGGFNA LVNVAGGFAW ETLEGGSVET WDRLYAMNLR SAVVASREAL PHLLRQPPGR IVNVGALAAQ KAGAGMGAYA ASKAGIARLT EALADELKDR NINVNAVLPN VLDTPANRAD MPDADATRWV STEALAAVIA FLLSDDAGAV TGACLPVAGR M
NT Sequence
ATGGACACGA GGCAGTTGGC GCACCGCACG ATAATCGTCA CGGGCGGGCT GGGCGCGTTG GGGCGCAGCG TCGGTGCAGT GCTGGCCGCA CGGGGCGCGC GTGTTGTGCT GATCGATCGC GCAGAGCCAA CCGCGGTAGC GGGTGTCGCC GCGGTCGTCG GCAAGGTCGA TCTGGGAGAG CGCGAGAGCA CCGCGCACGC TTTCGCGGAC GTGGCCCGGC AACTGGGCGG CTTCAACGCA CTTGTGAACG TCGCCGGCGG CTTCGCCTGG GAAACGCTGG AAGGCGGCAG CGTCGAAACA TGGGACCGCC TCTACGCGAT GAACCTGCGC AGCGCGGTCG TCGCGTCTCG CGAAGCGCTG CCCCATTTGC TGCGCCAGCC TCCCGGGCGC ATCGTCAACG TCGGCGCGCT CGCCGCGCAG AAGGCCGGCG CGGGCATGGG TGCCTATGCC GCCTCGAAGG CCGGTATCGC GCGTTTGACC GAAGCGCTCG CCGACGAGCT CAAGGATCGC AATATCAATG TGAACGCCGT GTTGCCGAAC GTTCTGGATA CTCCGGCAAA CCGCGCCGAC ATGCCGGATG CCGATGCGAC GCGCTGGGTA TCGACCGAAG CGTTGGCCGC CGTGATCGCC TTTCTGCTGT CCGACGACGC CGGTGCGGTA ACGGGCGCGT GCCTGCCCGT CGCGGGACGC ATG
Details for BuxeA.00010.u.B2.GE39422
HARVESTED ON: 8/5/2015
SEQUENCED ON: 8/13/2015
EXPECTED MW: 22kDa
OBSERVED MW: 22kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMA ARGARVVLID RAEPTAVAGV AAVVGKVDLG ERESTAHAFA DVARQLGGFN ALVNVAGGFA WETLEGGSVE TWDRLYAMNL RSAVVASREA LPHLLRQPPG RIVNVGALAA QKAGAGMGAY AASKAGIARL TEALADELKD RNINVNAVLP NVLDTPANRA DMPDADATRW VSTEALAAVI AFLLSDDAGA VTGACLPVAG RM
Validated NT Sequence
atcttactca cttacatgcg tcccgcgacg ggcaggcacg cgcccgttac cgcaccggcg tcgtcggaca gcagaaaggc gatcacggcg gccaacgctt cggtcgatac ccagcgcgtc gcatcggcat ccggcatgtc ggcgcggttt gccggagtat ccagaacgtt cggcaacacg gcgttcacat tgatattgcg atccttgagc tcgtcggcga gcgcttcggt caaacgcgcg ataccggcct tcgaggcggc ataggcaccc atgcccgcgc cggccttctg cgcggcgagc gcgccgacgt tgacgatgcg cccgggaggc tggcgcagca aatggggcag cgcttcgcga gacgcgacga ccgcgctgcg caggttcatc gcgtagaggc ggtcccatgt ttcgacgctg ccgccttcca gcgtttccca ggcgaagccg ccggcgacgt tcacaagtgc gttgaagccg cccagttgcc gggccacgtc cgcgaaagcg tgcgcggtgc tctcgcgctc tcccagatcg accttgccga cgaccgcggc gacacccgct accgcggttg gctctgcgcg atcgatcagc acaacacgcg cgccccgtgc ggccatatgg tggtggtggt ggtgagccat
Expected Protein Sequence
MAHHHHHHAA RGARVVLIDR AEPTAVAGVA AVVGKVDLGE RESTAHAFAD VARQLGGFNA LVNVAGGFAW ETLEGGSVET WDRLYAMNLR SAVVASREAL PHLLRQPPGR IVNVGALAAQ KAGAGMGAYA ASKAGIARLT EALADELKDR NINVNAVLPN VLDTPANRAD MPDADATRWV STEALAAVIA FLLSDDAGAV TGACLPVAGR M
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgccgcac ggggcgcgcg tgttgtgctg atcgatcgcg cagagccaac cgcggtagcg ggtgtcgccg cggtcgtcgg caaggtcgat ctgggagagc gcgagagcac cgcgcacgct ttcgcggacg tggcccggca actgggcggc ttcaacgcac ttgtgaacgt cgccggcggc ttcgcctggg aaacgctgga aggcggcagc gtcgaaacat gggaccgcct ctacgcgatg aacctgcgca gcgcggtcgt cgcgtctcgc gaagcgctgc cccatttgct gcgccagcct cccgggcgca tcgtcaacgt cggcgcgctc gccgcgcaga aggccggcgc gggcatgggt gcctatgccg cctcgaaggc cggtatcgcg cgtttgaccg aagcgctcgc cgacgagctc aaggatcgca atatcaatgt gaacgccgtg ttgccgaacg ttctggatac tccggcaaac cgcgccgaca tgccggatgc cgatgcgacg cgctgggtat cgaccgaagc gttggccgcc gtgatcgcct ttctgctgtc cgacgacgcc ggtgcggtaa cgggcgcgtg cctgcccgtc gcgggacgca tgtgagtaag ataggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaatt cttgaagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg cagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga ggcagctgcg gtaaagctca tcagcgtggt cgtgaagcga ttcacagatg tctgcctgtt catccgcgtc cagctcgttg agtttctcca gaagcgttaa tgtctggctt ctgataaagc gggccatgtt aagggcggtt ttttcctgtt tggtcactga tgcctccgtg taagggggat ttctgttcat gggggtaatg ataccgatga aacgagagag gatgctcacg atacgggtta ctgatgatga acatgcccgg ttactggaac gttgtgaggg taaacaactg gcggtatgga tgcggcggga ccagagaaaa atcactcagg gtcaatgcca gcgcttcgtt aatacagatg taggtgttcc acagggtagc cagcagcatc ctgcgatgca gatccggaac ataatggtgc agggcgctga cttccgcgtt tccagacttt acgaaacacg gaaaccgaag accattcatg ttgttgctca ggtcgcagac gttttgcagc agcagtcgct tcacgttcgc tcgcgtatcg gtgattcatt ctgctaacca gtaaggcaac cccgccagcc tagccgggtc ctcaacgaca ggagcacgat catgcgcacc cgtggccagg acccaacgct gcccgagatg cgccgcgtgc ggctgctgga gatggcggac gcgatggata tgttctgcca agggttggtt tgcgcattca cagttctccg caagaattga ttggctccaa ttcttggagt ggtgaatccg ttagcgaggt gccgccggct tccattcagg tcgaggtggc ccggctccat gcaccgcgac gcaacgcggg gaggcagaca aggtataggg cggcgcctac aatccatgcc aacccgttcc atgtgctcgc cgaggcggca taaatcgccg tgacgatcag cggtccagtg atcgaagtta ggctggtaag agccgcgagc gatccttgaa gctgtccctg atggtcgtca tctacctgcc tggacagcat ggcctgcaac gcgggcatcc cgatgccgcc ggaagcgaga agaatcataa tggggaaggc catccagcct cgcgtcgcga acgccagcaa gacgtagccc agcgcgtcgg ccgccatgcc ggcgataatg gcctgcttct cgccgaaacg tttggtggcg ggaccagtga cgaaggcttg agcgagggcg tgcaagattc cgaataccgc aagcgacagg ccgatcatcg tcgcgctcca gcgaaagcgg tcctcgccga aaatgaccca gagcgctgcc ggcacctgtc ctacgagttg catgataaag aagacagtca taagtgcggc gacgatagtc atgccccgcg cccaccggaa ggagctgact gggttgaagg ctctcaaggg catcggtcga cgctctccct tatgcgactc ctgcattagg aagcagccca gtagtaggtt gaggccgttg agcaccgccg ccgcaaggaa tggtgcatgc aaggagatgg cgcccaacag tcccccggcc acggggcctg ccaccatacc cacgccgaaa caagcgctca tgagcccgaa gtggcgagcc cgatcttccc catcggtgat gtcggcgata taggcgccag caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag aggatcgaga tctcgatccc gcgaaat