SSGCID: Target Page

BuxeA.00010.t

Putative short-chain dehydrogenase/reductase

CENTER ID:	BuxeA.00010.t
ORGANISM:	Burkholderia xenovorans LB400
ASSOCIATED DISEASE:
CURRENT STATUS:	in PDB
COMMUNITY REQUEST:	False
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BuxeA.00010.t.B1.GE39421	Full length( BuxeA.00010.t )		1	266

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Structures

5JY1

DEPOSITED: 5/13/2016

DETERMINATION: XRay

Diffraction Image

CLONE: BuxeA.00010.t.B1.GE39421

PROTEIN: BuxeA.00010.t.B1.PS37842

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|266265.5.peg.8350
RefSeq:	YP_555839.1
UniProt:	Q13HF0

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MGLLEQRVAL VTGAGGGIGR GVARSFGNEG AAVIIAEINE STGRQVEQEI REMGGRSLFV KTDVTSKASI EAAVRSAVEQ FGSLDILVNN AFVPTPNVLL EEKTDEMLEQ TLTTSLWATW WAMRAAFVPM RERRWGRIVN FYSIDTETGA WLHGDYNTAK AGIVGLTRSA ASEWGRFNIT VNAIAPTAMG ATFFELAAKN PEFAERSAAA RPLGRSGDPE QDIGPAAVFF ASEMSRFVTG ETLHVDGGLH LPGYNSRPAG IKPREY

NT Sequence

ATGGGACTGC TTGAGCAGCG AGTGGCACTC GTGACCGGAG CGGGCGGCGG AATTGGCCGC GGCGTTGCGC GCTCGTTCGG TAACGAAGGT GCAGCGGTGA TTATCGCGGA GATCAACGAA AGCACGGGCC GCCAGGTCGA GCAGGAAATC CGCGAGATGG GGGGCCGCTC GCTGTTCGTC AAAACCGACG TCACGAGCAA GGCGTCGATT GAAGCAGCGG TGCGCAGTGC CGTCGAGCAG TTCGGCAGTC TCGACATTCT CGTCAACAAC GCGTTTGTTC CGACGCCGAA TGTGCTACTC GAAGAGAAGA CCGACGAGAT GCTGGAGCAG ACGCTCACGA CCTCGTTGTG GGCGACCTGG TGGGCCATGC GCGCCGCGTT TGTGCCCATG CGCGAGCGGC GCTGGGGGCG CATCGTGAAC TTCTACTCGA TCGACACCGA GACAGGCGCA TGGCTCCATG GCGATTACAA CACGGCGAAG GCGGGCATCG TCGGGCTGAC TCGCAGCGCC GCATCGGAAT GGGGACGCTT CAACATCACC GTTAACGCGA TCGCGCCGAC GGCAATGGGT GCGACCTTCT TCGAGCTGGC GGCGAAGAAC CCGGAGTTTG CCGAACGCTC GGCCGCGGCG CGGCCGCTCG GGCGCAGCGG GGACCCGGAG CAGGACATCG GGCCTGCCGC GGTGTTCTTT GCGTCCGAGA TGTCACGCTT CGTTACGGGC GAAACACTGC ATGTCGACGG TGGCCTGCAT CTTCCGGGGT ACAACTCACG GCCAGCCGGG ATCAAGCCGC GTGAGTAT

Details for BuxeA.00010.t.B1.GE39421

HARVESTED ON:	8/5/2015
SEQUENCED ON:	8/13/2015
EXPECTED MW:	30kDa
OBSERVED MW:	30kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMG LLEQRVALVT GAGGGIGRGV ARSFGNEGAA VIIAEINEST GRQVEQEIRE MGGRSLFVKT DVTSKASIEA AVRSAVEQFG SLDILVNNAF VPTPNVLLEE KTDEMLEQTL TTSLWATWWA MRAAFVPMRE RRWGRIVNFY SIDTETGAWL HGDYNTAKAG IVGLTRSAAS EWGRFNITVN AIAPTAMGAT FFELAAKNPE FAERSAAARP LGRSGDPEQD IGPAAVFFAS EMSRFVTGET LHVDGGLHLP GYNSRPAGIK PREY

Validated NT Sequence

atcttactca cttaatactc acgcggcttg atcccggctg gccgtgagtt gtaccccgga agatgcaggc caccgtcgac atgcagtgtt tcgcccgtaa cgaagcgtga catctcggac gcaaagaaca ccgcggcagg cccgatgtcc tgctccgggt ccccgctgcg cccgagcggc cgcgccgcgg ccgagcgttc ggcaaactcc gggttcttcg ccgccagctc gaagaaggtc gcacccattg ccgtcggcgc gatcgcgtta acggtgatgt tgaagcgtcc ccattccgat gcggcgctgc gagtcagccc gacgatgccc gccttcgccg tgttgtaatc gccatggagc catgcgcctg tctcggtgtc gatcgagtag aagttcacga tgcgccccca gcgccgctcg cgcatgggca caaacgcggc gcgcatggcc caccaggtcg cccacaacga ggtcgtgagc gtctgctcca gcatctcgtc ggtcttctct tcgagtagca cattcggcgt cggaacaaac gcgttgttga cgagaatgtc gagactgccg aactgctcga cggcactgcg caccgctgct tcaatcgacg ccttgctcgt gacgtcggtt ttgacgaaca gcgagcggcc ccccatctcg cggatttcct gctcgacctg gcggcccgtg ctttcgttga tctccgcgat aatcaccgct gcaccttcgt taccgaacga gcgcgcaacg ccgcggccaa ttccgccgcc cgctccggtc acgagtgcca ctcgctgctc aagcagtccc atatggtggt ggtggtggtg agccat

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgggac tgcttgagca gcgagtggca ctcgtgaccg gagcgggcgg cggaattggc cgcggcgttg cgcgctcgtt cggtaacgaa ggtgcagcgg tgattatcgc ggagatcaac gaaagcacgg gccgccaggt cgagcaggaa atccgcgaga tggggggccg ctcgctgttc gtcaaaaccg acgtcacgag caaggcgtcg attgaagcag cggtgcgcag tgccgtcgag cagttcggca gtctcgacat tctcgtcaac aacgcgtttg ttccgacgcc gaatgtgcta ctcgaagaga agaccgacga gatgctggag cagacgctca cgacctcgtt gtgggcgacc tggtgggcca tgcgcgccgc gtttgtgccc atgcgcgagc ggcgctgggg gcgcatcgtg aacttctact cgatcgacac cgagacaggc gcatggctcc atggcgatta caacacggcg aaggcgggca tcgtcgggct gactcgcagc gccgcatcgg aatggggacg cttcaacatc accgttaacg cgatcgcgcc gacggcaatg ggtgcgacct tcttcgagct ggcggcgaag aacccggagt ttgccgaacg ctcggccgcg gcgcggccgc tcgggcgcag cggggacccg gagcaggaca tcgggcctgc cgcggtgttc tttgcgtccg agatgtcacg cttcgttacg ggcgaaacac tgcatgtcga cggtggcctg catcttccgg ggtacaactc acggccagcc gggatcaagc cgcgtgagta ttgagtaaga taggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaattc ttgaagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgc agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggccagga cccaacgctg cccgagatgc gccgcgtgcg gctgctggag atggcggacg cgatggatat gttctgccaa gggttggttt gcgcattcac agttctccgc aagaattgat tggctccaat tcttggagtg gtgaatccgt tagcgaggtg ccgccggctt ccattcaggt cgaggtggcc cggctccatg caccgcgacg caacgcgggg aggcagacaa ggtatagggc ggcgcctaca atccatgcca acccgttcca tgtgctcgcc gaggcggcat aaatcgccgt gacgatcagc ggtccagtga tcgaagttag gctggtaaga gccgcgagcg atccttgaag ctgtccctga tggtcgtcat ctacctgcct ggacagcatg gcctgcaacg cgggcatccc gatgccgccg gaagcgagaa gaatcataat ggggaaggcc atccagcctc gcgtcgcgaa cgccagcaag acgtagccca gcgcgtcggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaat