BuxeA.00010.l

Putative short-chain dehydrogenase/reductase

CENTER ID: BuxeA.00010.l
ORGANISM: Burkholderia xenovorans LB400
ASSOCIATED DISEASE:
CURRENT STATUS: expressed
COMMUNITY REQUEST: False
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BuxeA.00010.l.B2.GE39416 Mature protein( BuxeA.00010.l ) 28 273
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|266265.5.peg.8434
RefSeq: YP_555919.1
UniProt: Q13H70

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MKERLASKVA VITGAAQGIG ASVARSFAQN GCFVYVTDIN DELGRTVATS IGDCAAYLPL DVREEGDWQR VTTHVLEKHG RLDVLVNNAG ITGFEARVVP HDPEHASLEE WRAVHRTNLD GVFLGCKYAI RAMRRHGQGS IINISSRSGL VGIPGAAAYA SSKAAVRNHT KTVALYCAGQ NLEVRCNSIH PAAILTPIWE PMLGTDAGRE ERMAALVRDT PLRRFGTPEE VAALALLLAS DEATYITGSE FNIDGGLLAG SAATPAVIDD GEN
NT Sequence
ATGAAAGAAA GACTTGCTTC CAAGGTTGCG GTCATCACTG GGGCAGCCCA AGGAATAGGT GCGTCGGTTG CACGGTCATT CGCACAGAAC GGTTGCTTCG TCTACGTCAC TGATATCAAC GATGAGCTTG GCAGAACAGT GGCGACGTCG ATTGGCGATT GCGCTGCGTA TTTGCCACTT GACGTTCGAG AAGAAGGCGA TTGGCAGCGT GTAACGACGC ATGTCCTGGA AAAACACGGC CGACTGGACG TATTGGTCAA CAACGCAGGG ATTACTGGCT TTGAGGCTCG TGTCGTGCCG CATGATCCAG AGCACGCGAG CCTTGAGGAG TGGCGCGCTG TACATCGAAC GAACCTCGAC GGAGTGTTTC TCGGCTGCAA GTACGCCATA CGTGCCATGC GACGTCATGG ACAGGGCTCG ATCATAAACA TCTCTTCACG CTCTGGCCTG GTCGGCATTC CAGGTGCGGC GGCATATGCA TCGTCAAAGG CCGCAGTCCG CAACCACACA AAGACTGTTG CGCTCTACTG CGCGGGACAA AACCTCGAAG TTCGGTGCAA CTCGATTCAC CCAGCCGCCA TCCTCACGCC CATCTGGGAA CCCATGTTGG GCACCGACGC CGGCCGCGAG GAACGAATGG CTGCACTGGT CCGTGATACC CCACTCCGGC GGTTCGGCAC GCCTGAGGAA GTTGCCGCCC TCGCATTGTT GCTGGCATCG GATGAGGCGA CGTACATCAC CGGTAGTGAA TTCAACATTG ACGGTGGGCT CCTGGCCGGG TCGGCGGCAA CACCCGCTGT GATCGATGAC GGCGAAAAC
Details for BuxeA.00010.l.B2.GE39416
HARVESTED ON: 8/5/2015
SEQUENCED ON: 8/13/2015
EXPECTED MW: 27kDa
OBSERVED MW: 31kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY: 99
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMA QNGCFVYVTD INDELGRTVA TSIGDCAAYL PLDVREEGDW QRVTTHVLEK HGRLDVLVNN AGITGFEARV VPHDPEHASL EEWRAVHRTN LDGVFLGCKY AIRAMRRHGQ GSIINISSRS GLVGIPGAAA YASSKAAVRN HTKTVALYCA GQNLEVRCNS IHPAAILTPI WEPMLGTDAG REERMAALVR DTPLRRFGTP EEVAALALLL ASDEATYITG SEFNIDGGLL AGSAATPAXI DDGEN
Validated NT Sequence
atcttactca cttagttttc gccgtcatcg atnnnngcgg gtgttgccgc cgacccggcc aggagcccac cgtcaatgtt gaattcacta ccggtgatgt acgtcgcctc atccgatgcc agcaacaatg cgagggcggc aacttcctca ggcgtgccga accgccggag tggggtatca cggaccagtg cagccattcg ttcctcgcgg ccggcgtcgg tgcccaacat gggttcccag atgggcgtga ggatggcggc tgggtgaatc gagttgcacc gaacttcgag gttttgtccc gcgcagtaga gcgcaacagt ctttgtgtgg ttgcggactg cggcctttga cgatgcatat gccgccgcac ctggaatgcc gaccaggcca gagcgtgaag agatgtttat gatcgagccc tgtccatgac gtcgcatggc acgtatggcg tacttgcagc cgagaaacac tccgtcgagg ttcgttcgat gtacagcgcg ccactcctca aggctcgcgt gctctggatc atgcggcacg acacgagcct caaagccagt aatccctgcg ttgttgacca atacgtccag tcggccgtgt ttttccagga catgcgtcgt tacacgctgc caatcgcctt cttctcgaac gtcaagtggc aaatacgcag cgcaatcgcc aatcgacgtc gccactgttc tgccaagctc atcgttgata tcagtgacgt agacgaagca accgttctgt gccatatggt ggtggtggtg gtgagccat
Expected Protein Sequence
MAHHHHHHAQ NGCFVYVTDI NDELGRTVAT SIGDCAAYLP LDVREEGDWQ RVTTHVLEKH GRLDVLVNNA GITGFEARVV PHDPEHASLE EWRAVHRTNL DGVFLGCKYA IRAMRRHGQG SIINISSRSG LVGIPGAAAY ASSKAAVRNH TKTVALYCAG QNLEVRCNSI HPAAILTPIW EPMLGTDAGR EERMAALVRD TPLRRFGTPE EVAALALLLA SDEATYITGS EFNIDGGLLA GSAATPAVID DGEN
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgcacaga acggttgctt cgtctacgtc actgatatca acgatgagct tggcagaaca gtggcgacgt cgattggcga ttgcgctgcg tatttgccac ttgacgttcg agaagaaggc gattggcagc gtgtaacgac gcatgtcctg gaaaaacacg gccgactgga cgtattggtc aacaacgcag ggattactgg ctttgaggct cgtgtcgtgc cgcatgatcc agagcacgcg agccttgagg agtggcgcgc tgtacatcga acgaacctcg acggagtgtt tctcggctgc aagtacgcca tacgtgccat gcgacgtcat ggacagggct cgatcataaa catctcttca cgctctggcc tggtcggcat tccaggtgcg gcggcatatg catcgtcaaa ggccgcagtc cgcaaccaca caaagactgt tgcgctctac tgcgcgggac aaaacctcga agttcggtgc aactcgattc acccagccgc catcctcacg cccatctggg aacccatgtt gggcaccgac gccggccgcg aggaacgaat ggctgcactg gtccgtgata ccccactccg gcggttcggc acgcctgagg aagttgccgc cctcgcattg ttgctggcat cggatgaggc gacgtacatc accggtagtg aattcaacat tgacggtggg ctcctggccg ggtcggcggc aacacccgct gtgatcgatg acggcgaaaa ctgagtaaga taggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaattc ttgaagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgc agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggccagga cccaacgctg cccgagatgc gccgcgtgcg gctgctggag atggcggacg cgatggatat gttctgccaa gggttggttt gcgcattcac agttctccgc aagaattgat tggctccaat tcttggagtg gtgaatccgt tagcgaggtg ccgccggctt ccattcaggt cgaggtggcc cggctccatg caccgcgacg caacgcgggg aggcagacaa ggtatagggc ggcgcctaca atccatgcca acccgttcca tgtgctcgcc gaggcggcat aaatcgccgt gacgatcagc ggtccagtga tcgaagttag gctggtaaga gccgcgagcg atccttgaag ctgtccctga tggtcgtcat ctacctgcct ggacagcatg gcctgcaacg cgggcatccc gatgccgccg gaagcgagaa gaatcataat ggggaaggcc atccagcctc gcgtcgcgaa cgccagcaag acgtagccca gcgcgtcggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaat