SSGCID: Target Page

BuxeA.00010.k

Putative short-chain dehydrogenase/reductase

CENTER ID:	BuxeA.00010.k
ORGANISM:	Burkholderia xenovorans LB400
ASSOCIATED DISEASE:
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	False
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BuxeA.00010.k.B2.GE39415	Mature protein( BuxeA.00010.k )		26	256

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|266265.5.peg.8550
RefSeq:	YP_556016.1
UniProt:	Q13GX3

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MRLERKRTLI TAAASGMGAA GTRLFAEHGA RVAALDRDGA ALQRVVDDVK SRGGEARGFV VDLTHRQATL DTVREAVEWL GGIDVLWNHA GMPAPSNIED FEFEQYERSI NLNVTSAVVI SSAILPWMTA QKSGSIIFTS STSGLVGSAL SPLYSAFKSG IVGLTKGLAV RYAAQNVRVN ALCPGPVSTP MLHNDFIAGD ARFDKEENLK RVISVVPMGR MGEPVEIAHA ALWLASDESS FVTGVALPID GGQTAR

NT Sequence

ATGAGACTTG AAAGGAAACG AACATTGATT ACTGCAGCGG CATCGGGTAT GGGCGCTGCG GGCACGCGCC TGTTTGCTGA ACACGGTGCG CGCGTTGCCG CTCTCGACCG CGACGGAGCC GCCCTGCAAC GCGTGGTCGA TGACGTAAAG AGTCGGGGAG GCGAAGCCCG GGGCTTTGTG GTCGATCTGA CCCACCGGCA GGCGACGCTC GACACAGTGC GCGAGGCTGT CGAATGGCTC GGCGGAATTG ATGTTTTGTG GAATCACGCC GGGATGCCTG CACCCTCGAA CATCGAGGAC TTCGAATTCG AACAATACGA GCGCTCCATC AATCTGAACG TGACCTCGGC GGTGGTGATA TCCAGTGCAA TCCTGCCGTG GATGACTGCG CAGAAGAGCG GCTCGATTAT CTTCACTTCG TCGACGTCGG GGCTCGTTGG GTCAGCGTTA AGCCCGCTCT ACTCCGCTTT CAAATCCGGC ATTGTCGGTT TGACGAAGGG ACTGGCCGTC AGATACGCGG CGCAGAACGT CCGCGTCAAT GCATTGTGTC CGGGCCCGGT GTCAACGCCA ATGTTGCACA ACGACTTCAT CGCCGGCGAC GCCAGGTTCG ACAAGGAAGA AAACCTCAAG CGCGTCATCT CGGTCGTCCC CATGGGACGT ATGGGTGAGC CAGTCGAGAT CGCCCATGCC GCGTTGTGGC TCGCCTCCGA TGAATCGTCG TTTGTCACGG GCGTCGCCTT GCCGATCGAC GGTGGCCAGA CAGCCCGC

Details for BuxeA.00010.k.B2.GE39415

HARVESTED ON:	8/5/2015
SEQUENCED ON:	8/13/2015
EXPECTED MW:	26kDa
OBSERVED MW:	26kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Insoluble
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMA EHGARVAALD RDGAALQRVV DDVKSRGGEA RGFVVDLTHR QATLDTVREA VEWLGGIDVL WNHAGMPAPS NIEDFEFEQY ERSINLNVTS AVVISSAILP WMTAQKSGSI IFTSSTSGLV GSALSPLYSA FKSGIVGLTK GLAVRYAAQN VRVNALCPGP VSTPMLHNDF IAGDARFDKE ENLKRVISVV PMGRMGEPVE IAHAALWLAS DESSFVTGVA LPIDGGQTAR

Validated NT Sequence

atcttactca cttagcgggc tgtctggcca ccgtcgatcg gcaaggcgac gcccgtgaca aacgacgatt catcggaggc gagccacaac gcggcatggg cgatctcgac tggctcaccc atacgtccca tggggacgac cgagatgacg cgcttgaggt tttcttcctt gtcgaacctg gcgtcgccgg cgatgaagtc gttgtgcaac attggcgttg acaccgggcc cggacacaat gcattgacgc ggacgttctg cgccgcgtat ctgacggcca gtcccttcgt caaaccgaca atgccggatt tgaaagcgga gtagagcggg cttaacgctg acccaacgag ccccgacgtc gacgaagtga agataatcga gccgctcttc tgcgcagtca tccacggcag gattgcactg gatatcacca ccgccgaggt cacgttcaga ttgatggagc gctcgtattg ttcgaattcg aagtcctcga tgttcgaggg tgcaggcatc ccggcgtgat tccacaaaac atcaattccg ccgagccatt cgacagcctc gcgcactgtg tcgagcgtcg cctgccggtg ggtcagatcg accacaaagc cccgggcttc gcctccccga ctctttacgt catcgaccac gcgttgcagg gcggctccgt cgcggtcgag agcggcaacg cgcgcaccgt gttcagccat atggtggtgg tggtggtgag ccat

Expected Protein Sequence

MAHHHHHHAE HGARVAALDR DGAALQRVVD DVKSRGGEAR GFVVDLTHRQ ATLDTVREAV EWLGGIDVLW NHAGMPAPSN IEDFEFEQYE RSINLNVTSA VVISSAILPW MTAQKSGSII FTSSTSGLVG SALSPLYSAF KSGIVGLTKG LAVRYAAQNV RVNALCPGPV STPMLHNDFI AGDARFDKEE NLKRVISVVP MGRMGEPVEI AHAALWLASD ESSFVTGVAL PIDGGQTAR

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgctgaac acggtgcgcg cgttgccgct ctcgaccgcg acggagccgc cctgcaacgc gtggtcgatg acgtaaagag tcggggaggc gaagcccggg gctttgtggt cgatctgacc caccggcagg cgacgctcga cacagtgcgc gaggctgtcg aatggctcgg cggaattgat gttttgtgga atcacgccgg gatgcctgca ccctcgaaca tcgaggactt cgaattcgaa caatacgagc gctccatcaa tctgaacgtg acctcggcgg tggtgatatc cagtgcaatc ctgccgtgga tgactgcgca gaagagcggc tcgattatct tcacttcgtc gacgtcgggg ctcgttgggt cagcgttaag cccgctctac tccgctttca aatccggcat tgtcggtttg acgaagggac tggccgtcag atacgcggcg cagaacgtcc gcgtcaatgc attgtgtccg ggcccggtgt caacgccaat gttgcacaac gacttcatcg ccggcgacgc caggttcgac aaggaagaaa acctcaagcg cgtcatctcg gtcgtcccca tgggacgtat gggtgagcca gtcgagatcg cccatgccgc gttgtggctc gcctccgatg aatcgtcgtt tgtcacgggc gtcgccttgc cgatcgacgg tggccagaca gcccgctgag taagatagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aattcttgaa gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgcagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat acactccgct atcgctacgt gactgggtca tggctgcgcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgaggcagc tgcggtaaag ctcatcagcg tggtcgtgaa gcgattcaca gatgtctgcc tgttcatccg cgtccagctc gttgagtttc tccagaagcg ttaatgtctg gcttctgata aagcgggcca tgttaagggc ggttttttcc tgtttggtca ctgatgcctc cgtgtaaggg ggatttctgt tcatgggggt aatgataccg atgaaacgag agaggatgct cacgatacgg gttactgatg atgaacatgc ccggttactg gaacgttgtg agggtaaaca actggcggta tggatgcggc gggaccagag aaaaatcact cagggtcaat gccagcgctt cgttaataca gatgtaggtg ttccacaggg tagccagcag catcctgcga tgcagatccg gaacataatg gtgcagggcg ctgacttccg cgtttccaga ctttacgaaa cacggaaacc gaagaccatt catgttgttg ctcaggtcgc agacgttttg cagcagcagt cgcttcacgt tcgctcgcgt atcggtgatt cattctgcta accagtaagg caaccccgcc agcctagccg ggtcctcaac gacaggagca cgatcatgcg cacccgtggc caggacccaa cgctgcccga gatgcgccgc gtgcggctgc tggagatggc ggacgcgatg gatatgttct gccaagggtt ggtttgcgca ttcacagttc tccgcaagaa ttgattggct ccaattcttg gagtggtgaa tccgttagcg aggtgccgcc ggcttccatt caggtcgagg tggcccggct ccatgcaccg cgacgcaacg cggggaggca gacaaggtat agggcggcgc ctacaatcca tgccaacccg ttccatgtgc tcgccgaggc ggcataaatc gccgtgacga tcagcggtcc agtgatcgaa gttaggctgg taagagccgc gagcgatcct tgaagctgtc cctgatggtc gtcatctacc tgcctggaca gcatggcctg caacgcgggc atcccgatgc cgccggaagc gagaagaatc ataatgggga aggccatcca gcctcgcgtc gcgaacgcca gcaagacgta gcccagcgcg tcggccgcca tgccggcgat aatggcctgc ttctcgccga aacgtttggt ggcgggacca gtgacgaagg cttgagcgag ggcgtgcaag attccgaata ccgcaagcga caggccgatc atcgtcgcgc tccagcgaaa gcggtcctcg ccgaaaatga cccagagcgc tgccggcacc tgtcctacga gttgcatgat aaagaagaca gtcataagtg cggcgacgat agtcatgccc cgcgcccacc ggaaggagct gactgggttg aaggctctca agggcatcgg tcgacgctct cccttatgcg actcctgcat taggaagcag cccagtagta ggttgaggcc gttgagcacc gccgccgcaa ggaatggtgc atgcaaggag atggcgccca acagtccccc ggccacgggg cctgccacca tacccacgcc gaaacaagcg ctcatgagcc cgaagtggcg agcccgatct tccccatcgg tgatgtcggc gatataggcg ccagcaaccg cacctgtggc gccggtgatg ccggccacga tgcgtccggc gtagaggatc gagatctcga tcccgcgaaa t