BuxeA.00010.g
Putative short-chain dehydrogenase/reductase
| CENTER ID: | BuxeA.00010.g |
| ORGANISM: | Burkholderia xenovorans LB400 |
| ASSOCIATED DISEASE: | |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | True |
| NIH RISK GROUP: | 3 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
IIIB |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| BuxeA.00010.g.B1.GE39413 | Full length( BuxeA.00010.g ) | 1 | 266 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Structures
5JLA
DEPOSITED: 4/26/2016
DETERMINATION: XRay
CLONE: BuxeA.00010.g.B1.GE39413
PROTEIN: BuxeA.00010.g.B1.PS02594
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| OrthoMCL: | OG5_133244 |
| PATRIC ID: | fig|266265.5.peg.8614 |
| RefSeq: | YP_556079.1 |
| UniProt: | Q13GR0 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MTASAEFNGA IAVVTGAGSG IGRACAELLS RSGANVIVAD RDIEAATRVA RATGGKTLVL DVGEDASVTA AANEVRARYG VADVLVNCAG VLQRTLPPGE LAQREWDVVS RIDLRGTYLC CAAFGAPMAD RRRGSIVNIA SVAGMRSGPL HAYGPAKAGV ISLTETLAGE WGPKGVRVNC VSPGFTQTPA LERGFTTHTL KADVLRDAAA LGHIVSANEI AEAVVFLASE RASAITGVNL PVDAGYLIAG SWAAYGGLRR ESAGQD
NT Sequence
ATGACGGCCA GCGCTGAATT CAACGGCGCG ATCGCCGTGG TCACGGGGGC GGGCAGCGGC ATCGGCCGCG CCTGTGCGGA GTTGCTGAGC CGTAGCGGCG CGAACGTCAT CGTCGCCGAC CGGGACATTG AAGCGGCGAC GCGGGTAGCG CGCGCCACCG GCGGAAAAAC GCTGGTGCTG GACGTGGGCG AAGATGCGTC GGTGACGGCC GCGGCGAACG AAGTGCGCGC CCGGTACGGT GTGGCGGATG TGCTCGTCAA CTGCGCTGGC GTCCTGCAAC GGACACTCCC GCCCGGTGAG TTGGCGCAGC GCGAATGGGA CGTCGTCTCG CGCATCGACT TGCGCGGCAC ATACCTTTGC TGCGCCGCCT TCGGCGCGCC GATGGCCGAC CGCCGGCGCG GCAGCATCGT GAACATCGCG TCGGTGGCCG GGATGCGATC GGGTCCGCTA CACGCCTATG GACCGGCCAA AGCCGGCGTC ATCAGCCTCA CGGAAACGCT CGCGGGCGAG TGGGGACCGA AAGGCGTGCG GGTCAATTGC GTATCGCCAG GCTTCACGCA GACGCCCGCG CTCGAACGGG GTTTCACTAC CCATACCCTG AAAGCCGATG TGCTGCGCGA CGCGGCGGCG CTCGGCCATA TCGTCTCCGC AAACGAGATC GCCGAAGCGG TAGTCTTTCT CGCTTCGGAA CGGGCGAGCG CGATTACAGG CGTCAATCTC CCGGTCGATG CGGGCTATCT GATCGCGGGG TCATGGGCGG CGTACGGCGG CCTGCGGCGC GAGTCTGCCG GACAAGAT
Details for BuxeA.00010.g.B1.GE39413
| HARVESTED ON: | 8/5/2015 |
| SEQUENCED ON: | 8/13/2015 |
| EXPECTED MW: | 28kDa |
| OBSERVED MW: | 28kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Too many to count (100+) |
| TOTAL EXPRESSION LEVEL: | Moderate Expression |
| SOLUBLE EXPRESSION LEVEL | High Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | |
| PERCENT IDENTITY: | 99 |
| PERCENT COVERAGE: | 100 |
Validated AA Sequence
MAHHHHHHMT ASAEFNGAIA VVTGAGSGIG RACAELLSRS GANVIVADRD IEAATRVARA TGGKTLVLDV GEDASVTAAA NEVRARYGVA DVLVNCAGVL QRTLPPGELA QREWDVVSRI DLRGTYLCCA AFGAPMADRR RGSIVNIASV AGMRSGPLHA YGPAKAGVIS LTETLAGEWG PKGVRVNCVS PGFTQTPALE RGFTTHTLKA DVLRDAAALG HIVSANEIAE AVVFLASERA SAITGVNLPV DAGYLIAGSW AAYGGLRRXS AGQD
Validated NT Sequence
atcttactca cttaatcttg tccggcagac tngcgccgca ggccgccgta cgccgcccat gaccccgcga tcagatagcc cgcatcgacc gggagattga cgcctgtaat cgcgctcgcc cgttccgaag cgagaaagac taccgcttcg gcgatctcgt ttgcggagac gatatggccg agcgccgccg cgtcgcgcag cacatcggct ttcagggtat gggtagtgaa accccgttcg agcgcgggcg tctgcgtgaa gcctggcgat acgcaattga cccgcacgcc tttcggtccc cactcgcccg cgagcgtttc cgtgaggctg atgacgccgg ctttggccgg tccataggcg tgtagcggac ccgatcgcat cccggccacc gacgcgatgt tcacgatgct gccgcgccgg cggtcggcca tcggcgcgcc gaaggcggcg cagcaaaggt atgtgccgcg caagtcgatg cgcgagacga cgtcccattc gcgctgcgcc aactcaccgg gcgggagtgt ccgttgcagg acgccagcgc agttgacgag cacatccgcc acaccgtacc gggcgcgcac ttcgttcgcc gcggccgtca ccgacgcatc ttcgcccacg tccagcacca gcgtttttcc gccggtggcg cgcgctaccc gcgtcgccgc ttcaatgtcc cggtcggcga cgatgacgtt cgcgccgcta cggctcagca actccgcaca ggcgcggccg atgccgctgc ccgcccccgt gaccacggcg atcgcgccgt tgaattcagc gctggccgtc atatggtggt ggtggtggtg agccat
Expected Protein Sequence
MAHHHHHHMT ASAEFNGAIA VVTGAGSGIG RACAELLSRS GANVIVADRD IEAATRVARA TGGKTLVLDV GEDASVTAAA NEVRARYGVA DVLVNCAGVL QRTLPPGELA QREWDVVSRI DLRGTYLCCA AFGAPMADRR RGSIVNIASV AGMRSGPLHA YGPAKAGVIS LTETLAGEWG PKGVRVNCVS PGFTQTPALE RGFTTHTLKA DVLRDAAALG HIVSANEIAE AVVFLASERA SAITGVNLPV DAGYLIAGSW AAYGGLRRES AGQD
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgacgg ccagcgctga attcaacggc gcgatcgccg tggtcacggg ggcgggcagc ggcatcggcc gcgcctgtgc ggagttgctg agccgtagcg gcgcgaacgt catcgtcgcc gaccgggaca ttgaagcggc gacgcgggta gcgcgcgcca ccggcggaaa aacgctggtg ctggacgtgg gcgaagatgc gtcggtgacg gccgcggcga acgaagtgcg cgcccggtac ggtgtggcgg atgtgctcgt caactgcgct ggcgtcctgc aacggacact cccgcccggt gagttggcgc agcgcgaatg ggacgtcgtc tcgcgcatcg acttgcgcgg cacatacctt tgctgcgccg ccttcggcgc gccgatggcc gaccgccggc gcggcagcat cgtgaacatc gcgtcggtgg ccgggatgcg atcgggtccg ctacacgcct atggaccggc caaagccggc gtcatcagcc tcacggaaac gctcgcgggc gagtggggac cgaaaggcgt gcgggtcaat tgcgtatcgc caggcttcac gcagacgccc gcgctcgaac ggggtttcac tacccatacc ctgaaagccg atgtgctgcg cgacgcggcg gcgctcggcc atatcgtctc cgcaaacgag atcgccgaag cggtagtctt tctcgcttcg gaacgggcga gcgcgattac aggcgtcaat ctcccggtcg atgcgggcta tctgatcgcg gggtcatggg cggcgtacgg cggcctgcgg cgcgagtctg ccggacaaga ttgagtaaga taggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaattc ttgaagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgc agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggccagga cccaacgctg cccgagatgc gccgcgtgcg gctgctggag atggcggacg cgatggatat gttctgccaa gggttggttt gcgcattcac agttctccgc aagaattgat tggctccaat tcttggagtg gtgaatccgt tagcgaggtg ccgccggctt ccattcaggt cgaggtggcc cggctccatg caccgcgacg caacgcgggg aggcagacaa ggtatagggc ggcgcctaca atccatgcca acccgttcca tgtgctcgcc gaggcggcat aaatcgccgt gacgatcagc ggtccagtga tcgaagttag gctggtaaga gccgcgagcg atccttgaag ctgtccctga tggtcgtcat ctacctgcct ggacagcatg gcctgcaacg cgggcatccc gatgccgccg gaagcgagaa gaatcataat ggggaaggcc atccagcctc gcgtcgcgaa cgccagcaag acgtagccca gcgcgtcggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaat