BuxeA.00010.c
Putative short-chain dehydrogenase/reductase
| CENTER ID: | BuxeA.00010.c |
| ORGANISM: | Burkholderia xenovorans LB400 |
| ASSOCIATED DISEASE: | |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | True |
| NIH RISK GROUP: | 3 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
IIIB |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| BuxeA.00010.c.B1.GE39410 | Full length( BuxeA.00010.c ) | 1 | 268 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| BuxeA.00010.c.B1.PS02595 | Full length( BuxeA.00010.c ) | 1 | 268 |
Structures
5JC8
DEPOSITED: 4/14/2016
DETERMINATION: XRay
CLONE: BuxeA.00010.c.B1.GE39410
PROTEIN: BuxeA.00010.c.B1.PS02595
Publications by SSGCID
Crystal structure of a putative short-chain dehydrogenase/reductase from Paraburkholderia xenovorans
Myler PJ, Staker BL, Subramanian S, Mayclin SJ, Asojo OA, Conrady DG, Davidson J, Nicholas K
Acta Crystallogr. Sect. F Struct. Biol. Cryst. Commun. - 2022
volume 78, issue Pt 1, pages 25-30
PMID: 34981772; PMCID: PMC8725002
PDB:
5JC8
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| OrthoMCL: | OG5_129138 |
| PATRIC ID: | fig|266265.5.peg.8733 |
| RefSeq: | YP_556196.1 |
| UniProt: | Q13GE3 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSSAGRLQGK VALVTGAGCI GPGWGNGRAI AVRFAEEGAH VIAVDRDLAS MDATLELVRA AGGSVTPCLC DVTDSASVER LVADSVARCG RVDILVNNVG APSPGGPVAL DEAQWAMQLE LNLTTAFLMC KYVLPVMEQQ GGGAIVNIAS TSGIRWTGAA QVGYAAAKAG MIQMGRVVAV EYAAKNVRVN SVVPGLLHTP MVDTKIAHNQ AGGDVELLLR KRQARIPMPF MGDGRDTANA ALFLASDEAR FVTGTEIVVD GGMSARCD
NT Sequence
ATGAGTTCAG CAGGAAGATT GCAGGGAAAG GTCGCGCTTG TCACGGGTGC CGGATGCATC GGCCCGGGTT GGGGCAACGG ACGCGCGATC GCCGTTCGCT TTGCCGAGGA AGGCGCGCAT GTGATTGCCG TTGATCGTGA CCTGGCCTCG ATGGACGCGA CGCTCGAACT CGTCCGCGCT GCGGGCGGGA GCGTAACGCC CTGCCTGTGC GACGTGACTG ACAGTGCGTC GGTCGAGCGC CTCGTCGCCG ACAGCGTGGC ACGCTGCGGA CGCGTCGATA TCCTCGTGAA CAATGTCGGC GCTCCGTCGC CGGGCGGACC GGTTGCGCTT GACGAGGCGC AGTGGGCGAT GCAACTCGAA CTGAATCTGA CGACGGCATT CCTGATGTGC AAATACGTGC TGCCGGTGAT GGAACAGCAG GGCGGCGGCG CGATCGTCAA TATCGCCTCC ACCTCGGGCA TCCGCTGGAC GGGCGCGGCG CAGGTCGGCT ATGCCGCCGC GAAGGCCGGC ATGATCCAGA TGGGCCGCGT CGTGGCCGTC GAATATGCGG CGAAGAACGT TCGTGTCAAT TCGGTAGTGC CCGGTCTGCT GCATACGCCA ATGGTCGATA CGAAGATCGC ACACAACCAG GCCGGCGGCG ATGTCGAGTT GCTGCTCAGG AAGCGCCAGG CGCGCATTCC AATGCCTTTC ATGGGAGACG GCCGCGACAC GGCCAATGCG GCGCTCTTTC TTGCATCGGA TGAAGCGCGT TTTGTTACCG GCACCGAAAT CGTGGTCGAC GGCGGGATGA GCGCCCGCTG CGAT
Details for BuxeA.00010.c.B1.GE39410
| HARVESTED ON: | 8/5/2015 |
| SEQUENCED ON: | 8/13/2015 |
| EXPECTED MW: | 29kDa |
| OBSERVED MW: | 29kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Too many to count (100+) |
| TOTAL EXPRESSION LEVEL: | Moderate Expression |
| SOLUBLE EXPRESSION LEVEL | High Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | |
| PERCENT IDENTITY: | 99 |
| PERCENT COVERAGE: | 100 |
Validated AA Sequence
MAHHHHHHMS SAGRLQGKVA LVTGAGCIGP GWGNGRAIAV RFAEEGAHVI AVDRDLASMD ATLELVRAAG GSVTPCLCDV TDSASVERLV ADSVARCGRV DILVNNVGAP SPGGPVALDE AQWAMQLELN LTTAFLMCKY VLPVMEQQGG GAIVNIASTS GIRWTGAAQV GYAAAKAGMI QMGRVVAVEY AAKNVRVNSV VPGLLHTPMV DTKIAHNQAG GDVELLLRKR QARIPMPFMG DGRDTANAAL FLASDEARFV TGTEIVVDXG MSARCD
Validated NT Sequence
atcttactca cttaatcgca gcgggcgctc atcccnnngt cgaccacgat ttcggtgccg gtaacaaaac gcgcttcatc cgatgcaaga aagagcgccg cattggccgt gtcgcggccg tctcccatga aaggcattgg aatgcgcgcc tggcgcttcc tgagcagcaa ctcgacatcg ccgccggcct ggttgtgtgc gatcttcgta tcgaccattg gcgtatgcag cagaccgggc actaccgaat tgacacgaac gttcttcgcc gcatattcga cggccacgac gcggcccatc tggatcatgc cggccttcgc ggcggcatag ccgacctgcg ccgcgcccgt ccagcggatg cccgaggtgg aggcgatatt gacgatcgcg ccgccgccct gctgttccat caccggcagc acgtatttgc acatcaggaa tgccgtcgtc agattcagtt cgagttgcat cgcccactgc gcctcgtcaa gcgcaaccgg tccgcccggc gacggagcgc cgacattgtt cacgaggata tcgacgcgtc cgcagcgtgc cacgctgtcg gcgacgaggc gctcgaccga cgcactgtca gtcacgtcgc acaggcaggg cgttacgctc ccgcccgcag cgcggacgag ttcgagcgtc gcgtccatcg aggccaggtc acgatcaacg gcaatcacat gcgcgccttc ctcggcaaag cgaacggcga tcgcgcgtcc gttgccccaa cccgggccga tgcatccggc acccgtgaca agcgcgacct ttccctgcaa tcttcctgct gaactcatat ggtggtggtg gtggtgagcc at
Expected Protein Sequence
MAHHHHHHMS SAGRLQGKVA LVTGAGCIGP GWGNGRAIAV RFAEEGAHVI AVDRDLASMD ATLELVRAAG GSVTPCLCDV TDSASVERLV ADSVARCGRV DILVNNVGAP SPGGPVALDE AQWAMQLELN LTTAFLMCKY VLPVMEQQGG GAIVNIASTS GIRWTGAAQV GYAAAKAGMI QMGRVVAVEY AAKNVRVNSV VPGLLHTPMV DTKIAHNQAG GDVELLLRKR QARIPMPFMG DGRDTANAAL FLASDEARFV TGTEIVVDGG MSARCD
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgagtt cagcaggaag attgcaggga aaggtcgcgc ttgtcacggg tgccggatgc atcggcccgg gttggggcaa cggacgcgcg atcgccgttc gctttgccga ggaaggcgcg catgtgattg ccgttgatcg tgacctggcc tcgatggacg cgacgctcga actcgtccgc gctgcgggcg ggagcgtaac gccctgcctg tgcgacgtga ctgacagtgc gtcggtcgag cgcctcgtcg ccgacagcgt ggcacgctgc ggacgcgtcg atatcctcgt gaacaatgtc ggcgctccgt cgccgggcgg accggttgcg cttgacgagg cgcagtgggc gatgcaactc gaactgaatc tgacgacggc attcctgatg tgcaaatacg tgctgccggt gatggaacag cagggcggcg gcgcgatcgt caatatcgcc tccacctcgg gcatccgctg gacgggcgcg gcgcaggtcg gctatgccgc cgcgaaggcc ggcatgatcc agatgggccg cgtcgtggcc gtcgaatatg cggcgaagaa cgttcgtgtc aattcggtag tgcccggtct gctgcatacg ccaatggtcg atacgaagat cgcacacaac caggccggcg gcgatgtcga gttgctgctc aggaagcgcc aggcgcgcat tccaatgcct ttcatgggag acggccgcga cacggccaat gcggcgctct ttcttgcatc ggatgaagcg cgttttgtta ccggcaccga aatcgtggtc gacggcggga tgagcgcccg ctgcgattga gtaagatagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaattcttga agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgcagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatata tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag ctgcggtaaa gctcatcagc gtggtcgtga agcgattcac agatgtctgc ctgttcatcc gcgtccagct cgttgagttt ctccagaagc gttaatgtct ggcttctgat aaagcgggcc atgttaaggg cggttttttc ctgtttggtc actgatgcct ccgtgtaagg gggatttctg ttcatggggg taatgatacc gatgaaacga gagaggatgc tcacgatacg ggttactgat gatgaacatg cccggttact ggaacgttgt gagggtaaac aactggcggt atggatgcgg cgggaccaga gaaaaatcac tcagggtcaa tgccagcgct tcgttaatac agatgtaggt gttccacagg gtagccagca gcatcctgcg atgcagatcc ggaacataat ggtgcagggc gctgacttcc gcgtttccag actttacgaa acacggaaac cgaagaccat tcatgttgtt gctcaggtcg cagacgtttt gcagcagcag tcgcttcacg ttcgctcgcg tatcggtgat tcattctgct aaccagtaag gcaaccccgc cagcctagcc gggtcctcaa cgacaggagc acgatcatgc gcacccgtgg ccaggaccca acgctgcccg agatgcgccg cgtgcggctg ctggagatgg cggacgcgat ggatatgttc tgccaagggt tggtttgcgc attcacagtt ctccgcaaga attgattggc tccaattctt ggagtggtga atccgttagc gaggtgccgc cggcttccat tcaggtcgag gtggcccggc tccatgcacc gcgacgcaac gcggggaggc agacaaggta tagggcggcg cctacaatcc atgccaaccc gttccatgtg ctcgccgagg cggcataaat cgccgtgacg atcagcggtc cagtgatcga agttaggctg gtaagagccg cgagcgatcc ttgaagctgt ccctgatggt cgtcatctac ctgcctggac agcatggcct gcaacgcggg catcccgatg ccgccggaag cgagaagaat cataatgggg aaggccatcc agcctcgcgt cgcgaacgcc agcaagacgt agcccagcgc gtcggccgcc atgccggcga taatggcctg cttctcgccg aaacgtttgg tggcgggacc agtgacgaag gcttgagcga gggcgtgcaa gattccgaat accgcaagcg acaggccgat catcgtcgcg ctccagcgaa agcggtcctc gccgaaaatg acccagagcg ctgccggcac ctgtcctacg agttgcatga taaagaagac agtcataagt gcggcgacga tagtcatgcc ccgcgcccac cggaaggagc tgactgggtt gaaggctctc aagggcatcg gtcgacgctc tcccttatgc gactcctgca ttaggaagca gcccagtagt aggttgaggc cgttgagcac cgccgccgca aggaatggtg catgcaagga gatggcgccc aacagtcccc cggccacggg gcctgccacc atacccacgc cgaaacaagc gctcatgagc ccgaagtggc gagcccgatc ttccccatcg gtgatgtcgg cgatataggc gccagcaacc gcacctgtgg cgccggtgat gccggccacg atgcgtccgg cgtagaggat cgagatctcg atcccgcgaa at
Details for BuxeA.00010.c.B1.PS02595
| PURIFICATION DATe: | 1/27/2016 |
| CONCENTRATION: | 45mg/ml |
| OBSERVED MW: | 28kDa |
| EXPRESSION LEVEL: | Low Expression |
| PROTEIN PURIFICATION BUFFER: | SEC Buffer |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 6 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | 99 |
| PERCENT COVERAGE: | 100 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMS SAGRLQGKVA LVTGAGCIGP GWGNGRAIAV RFAEEGAHVI AVDRDLASMD ATLELVRAAG GSVTPCLCDV TDSASVERLV ADSVARCGRV DILVNNVGAP SPGGPVALDE AQWAMQLELN LTTAFLMCKY VLPVMEQQGG GAIVNIASTS GIRWTGAAQV GYAAAKAGMI QMGRVVAVEY AAKNVRVNSV VPGLLHTPMV DTKIAHNQAG GDVELLLRKR QARIPMPFMG DGRDTANAAL FLASDEARFV TGTEIVVDXG MSARCD
Validated NT Sequence
atcttactca cttaatcgca gcgggcgctc atcccnnngt cgaccacgat ttcggtgccg gtaacaaaac gcgcttcatc cgatgcaaga aagagcgccg cattggccgt gtcgcggccg tctcccatga aaggcattgg aatgcgcgcc tggcgcttcc tgagcagcaa ctcgacatcg ccgccggcct ggttgtgtgc gatcttcgta tcgaccattg gcgtatgcag cagaccgggc actaccgaat tgacacgaac gttcttcgcc gcatattcga cggccacgac gcggcccatc tggatcatgc cggccttcgc ggcggcatag ccgacctgcg ccgcgcccgt ccagcggatg cccgaggtgg aggcgatatt gacgatcgcg ccgccgccct gctgttccat caccggcagc acgtatttgc acatcaggaa tgccgtcgtc agattcagtt cgagttgcat cgcccactgc gcctcgtcaa gcgcaaccgg tccgcccggc gacggagcgc cgacattgtt cacgaggata tcgacgcgtc cgcagcgtgc cacgctgtcg gcgacgaggc gctcgaccga cgcactgtca gtcacgtcgc acaggcaggg cgttacgctc ccgcccgcag cgcggacgag ttcgagcgtc gcgtccatcg aggccaggtc acgatcaacg gcaatcacat gcgcgccttc ctcggcaaag cgaacggcga tcgcgcgtcc gttgccccaa cccgggccga tgcatccggc acccgtgaca agcgcgacct ttccctgcaa tcttcctgct gaactcatat ggtggtggtg gtggtgagcc at
Expressed Protein Sequence
MAHHHHHHMS SAGRLQGKVA LVTGAGCIGP GWGNGRAIAV RFAEEGAHVI AVDRDLASMD ATLELVRAAG GSVTPCLCDV TDSASVERLV ADSVARCGRV DILVNNVGAP SPGGPVALDE AQWAMQLELN LTTAFLMCKY VLPVMEQQGG GAIVNIASTS GIRWTGAAQV GYAAAKAGMI QMGRVVAVEY AAKNVRVNSV VPGLLHTPMV DTKIAHNQAG GDVELLLRKR QARIPMPFMG DGRDTANAAL FLASDEARFV TGTEIVVDGG MSARCD
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGAGTT CAGCAGGAAG ATTGCAGGGA AAGGTCGCGC TTGTCACGGG TGCCGGATGC ATCGGCCCGG GTTGGGGCAA CGGACGCGCG ATCGCCGTTC GCTTTGCCGA GGAAGGCGCG CATGTGATTG CCGTTGATCG TGACCTGGCC TCGATGGACG CGACGCTCGA ACTCGTCCGC GCTGCGGGCG GGAGCGTAAC GCCCTGCCTG TGCGACGTGA CTGACAGTGC GTCGGTCGAG CGCCTCGTCG CCGACAGCGT GGCACGCTGC GGACGCGTCG ATATCCTCGT GAACAATGTC GGCGCTCCGT CGCCGGGCGG ACCGGTTGCG CTTGACGAGG CGCAGTGGGC GATGCAACTC GAACTGAATC TGACGACGGC ATTCCTGATG TGCAAATACG TGCTGCCGGT GATGGAACAG CAGGGCGGCG GCGCGATCGT CAATATCGCC TCCACCTCGG GCATCCGCTG GACGGGCGCG GCGCAGGTCG GCTATGCCGC CGCGAAGGCC GGCATGATCC AGATGGGCCG CGTCGTGGCC GTCGAATATG CGGCGAAGAA CGTTCGTGTC AATTCGGTAG TGCCCGGTCT GCTGCATACG CCAATGGTCG ATACGAAGAT CGCACACAAC CAGGCCGGCG GCGATGTCGA GTTGCTGCTC AGGAAGCGCC AGGCGCGCAT TCCAATGCCT TTCATGGGAG ACGGCCGCGA CACGGCCAAT GCGGCGCTCT TTCTTGCATC GGATGAAGCG CGTTTTGTTA CCGGCACCGA AATCGTGGTC GACGGCGGGA TGAGCGCCCG CTGCGATTGA GTAAGATAGG ATCCGGCTGC TAACAAAGCC CGAAAGGAAG CTGAGTTGGC TGCTGCCACC GCTGAGCAAT AACTAGCATA ACCCCTTGGG GCCTCTAAAC GGGTCTTGAG GGGTTTTTTG CTGAAAGGAG GAACTATATC CGGATATCCA CAGGACGGGT GTGGTCGCCA TGATCGCGTA GTCGATAGTG GCTCCAAGTA GCGAAGCGAG CAGGACTGGG CGGCGGCCAA AGCGGTCGGA CAGTGCTCCG AGAACGGGTG CGCATAGAAA TTGCATCAAC GCATATAGCG CTAGCAGCAC GCCATAGTGA CTGGCGATGC TGTCGGAATG GACGATATCC CGCAAGAGGC CCGGCAGTAC CGGCATAACC AAGCCTATGC CTACAGCATC CAGGGTGACG GTGCCGAGGA TGACGATGAG CGCATTGTTA GATTTCATAC ACGGTGCCTG ACTGCGTTAG CAATTTAACT GTGATAAACT ACCGCATTAA AGCTTATCGA TGATAAGCTG TCAAACATGA GAATTCTTGA AGACGAAAGG GCCTCGTGAT ACGCCTATTT TTATAGGTTA ATGTCATGAT AATAATGGTT TCTTAGACGT CAGGTGGCAC TTTTCGGGGA AATGTGCGCG GAACCCCTAT TTGTTTATTT TTCTAAATAC ATTCAAATAT GTATCCGCTC ATGAGACAAT AACCCTGATA AATGCTTCAA TAATATTGAA AAAGGAAGAG TATGAGTATT CAACATTTCC GTGTCGCCCT TATTCCCTTT TTTGCGGCAT TTTGCCTTCC TGTTTTTGCT CACCCAGAAA CGCTGGTGAA AGTAAAAGAT GCTGAAGATC AGTTGGGTGC ACGAGTGGGT TACATCGAAC TGGATCTCAA CAGCGGTAAG ATCCTTGAGA GTTTTCGCCC CGAAGAACGT TTTCCAATGA TGAGCACTTT TAAAGTTCTG CTATGTGGCG CGGTATTATC CCGTGTTGAC GCCGGGCAAG AGCAACTCGG TCGCCGCATA CACTATTCTC AGAATGACTT GGTTGAGTAC TCACCAGTCA CAGAAAAGCA TCTTACGGAT GGCATGACAG TAAGAGAATT ATGCAGTGCT GCCATAACCA TGAGTGATAA CACTGCGGCC AACTTACTTC TGACAACGAT CGGAGGACCG AAGGAGCTAA CCGCTTTTTT GCACAACATG GGGGATCATG TAACTCGCCT TGATCGTTGG GAACCGGAGC TGAATGAAGC CATACCAAAC GACGAGCGTG ACACCACGAT GCCTGCAGCA ATGGCAACAA CGTTGCGCAA ACTATTAACT GGCGAACTAC TTACTCTAGC TTCCCGGCAA CAATTAATAG ACTGGATGGA GGCGGATAAA GTTGCAGGAC CACTTCTGCG CTCGGCCCTT CCGGCTGGCT GGTTTATTGC TGATAAATCT GGAGCCGGTG AGCGTGGGTC TCGCGGTATC ATTGCAGCAC TGGGGCCAGA TGGTAAGCCC TCCCGTATCG TAGTTATCTA CACGACGGGG AGTCAGGCAA CTATGGATGA ACGAAATAGA CAGATCGCTG AGATAGGTGC CTCACTGATT AAGCATTGGT AACTGTCAGA CCAAGTTTAC TCATATATAC TTTAGATTGA TTTAAAACTT CATTTTTAAT TTAAAAGGAT CTAGGTGAAG ATCCTTTTTG ATAATCTCAT GACCAAAATC CCTTAACGTG AGTTTTCGTT CCACTGAGCG TCAGACCCCG TAGAAAAGAT CAAAGGATCT TCTTGAGATC CTTTTTTTCT GCGCGTAATC TGCTGCTTGC AAACAAAAAA ACCACCGCTA CCAGCGGTGG TTTGTTTGCC GGATCAAGAG CTACCAACTC TTTTTCCGAA GGTAACTGGC TTCAGCAGAG CGCAGATACC AAATACTGTC CTTCTAGTGT AGCCGTAGTT AGGCCACCAC TTCAAGAACT CTGTAGCACC GCCTACATAC CTCGCTCTGC TAATCCTGTT ACCAGTGGCT GCTGCCAGTG GCGATAAGTC GTGTCTTACC GGGTTGGACT CAAGACGATA GTTACCGGAT AAGGCGCAGC GGTCGGGCTG AACGGGGGGT TCGTGCACAC AGCCCAGCTT GGAGCGAACG ACCTACACCG AACTGAGATA CCTACAGCGT GAGCTATGAG AAAGCGCCAC GCTTCCCGAA GGGAGAAAGG CGGACAGGTA TCCGGTAAGC GGCAGGGTCG GAACAGGAGA GCGCACGAGG GAGCTTCCAG GGGGAAACGC CTGGTATCTT TATAGTCCTG TCGGGTTTCG CCACCTCTGA CTTGAGCGTC GATTTTTGTG ATGCTCGTCA GGGGGGCGGA GCCTATGGAA AAACGCCAGC AACGCGGCCT TTTTACGGTT CCTGGCCTTT TGCTGGCCTT TTGCTCACAT GTTCTTTCCT GCGTTATCCC CTGATTCTGT GGATAACCGT ATTACCGCCT TTGAGTGAGC TGATACCGCT CGCCGCAGCC GAACGACCGA GCGCAGCGAG TCAGTGAGCG AGGAAGCGGA AGAGCGCCTG ATGCGGTATT TTCTCCTTAC GCATCTGTGC GGTATTTCAC ACCGCATATA TGGTGCACTC TCAGTACAAT CTGCTCTGAT GCCGCATAGT TAAGCCAGTA TACACTCCGC TATCGCTACG TGACTGGGTC ATGGCTGCGC CCCGACACCC GCCAACACCC GCTGACGCGC CCTGACGGGC TTGTCTGCTC CCGGCATCCG CTTACAGACA AGCTGTGACC GTCTCCGGGA GCTGCATGTG TCAGAGGTTT TCACCGTCAT CACCGAAACG CGCGAGGCAG CTGCGGTAAA GCTCATCAGC GTGGTCGTGA AGCGATTCAC AGATGTCTGC CTGTTCATCC GCGTCCAGCT CGTTGAGTTT CTCCAGAAGC GTTAATGTCT GGCTTCTGAT AAAGCGGGCC ATGTTAAGGG CGGTTTTTTC CTGTTTGGTC ACTGATGCCT CCGTGTAAGG GGGATTTCTG TTCATGGGGG TAATGATACC GATGAAACGA GAGAGGATGC TCACGATACG GGTTACTGAT GATGAACATG CCCGGTTACT GGAACGTTGT GAGGGTAAAC AACTGGCGGT ATGGATGCGG CGGGACCAGA GAAAAATCAC TCAGGGTCAA TGCCAGCGCT TCGTTAATAC AGATGTAGGT GTTCCACAGG GTAGCCAGCA GCATCCTGCG ATGCAGATCC GGAACATAAT GGTGCAGGGC GCTGACTTCC GCGTTTCCAG ACTTTACGAA ACACGGAAAC CGAAGACCAT TCATGTTGTT GCTCAGGTCG CAGACGTTTT GCAGCAGCAG TCGCTTCACG TTCGCTCGCG TATCGGTGAT TCATTCTGCT AACCAGTAAG GCAACCCCGC CAGCCTAGCC GGGTCCTCAA CGACAGGAGC ACGATCATGC GCACCCGTGG CCAGGACCCA ACGCTGCCCG AGATGCGCCG CGTGCGGCTG CTGGAGATGG CGGACGCGAT GGATATGTTC TGCCAAGGGT TGGTTTGCGC ATTCACAGTT CTCCGCAAGA ATTGATTGGC TCCAATTCTT GGAGTGGTGA ATCCGTTAGC GAGGTGCCGC CGGCTTCCAT TCAGGTCGAG GTGGCCCGGC TCCATGCACC GCGACGCAAC GCGGGGAGGC AGACAAGGTA TAGGGCGGCG CCTACAATCC ATGCCAACCC GTTCCATGTG CTCGCCGAGG CGGCATAAAT CGCCGTGACG ATCAGCGGTC CAGTGATCGA AGTTAGGCTG GTAAGAGCCG CGAGCGATCC TTGAAGCTGT CCCTGATGGT CGTCATCTAC CTGCCTGGAC AGCATGGCCT GCAACGCGGG CATCCCGATG CCGCCGGAAG CGAGAAGAAT CATAATGGGG AAGGCCATCC AGCCTCGCGT CGCGAACGCC AGCAAGACGT AGCCCAGCGC GTCGGCCGCC ATGCCGGCGA TAATGGCCTG CTTCTCGCCG AAACGTTTGG TGGCGGGACC AGTGACGAAG GCTTGAGCGA GGGCGTGCAA GATTCCGAAT ACCGCAAGCG ACAGGCCGAT CATCGTCGCG CTCCAGCGAA AGCGGTCCTC GCCGAAAATG ACCCAGAGCG CTGCCGGCAC CTGTCCTACG AGTTGCATGA TAAAGAAGAC AGTCATAAGT GCGGCGACGA TAGTCATGCC CCGCGCCCAC CGGAAGGAGC TGACTGGGTT GAAGGCTCTC AAGGGCATCG GTCGACGCTC TCCCTTATGC GACTCCTGCA TTAGGAAGCA GCCCAGTAGT AGGTTGAGGC CGTTGAGCAC CGCCGCCGCA AGGAATGGTG CATGCAAGGA GATGGCGCCC AACAGTCCCC CGGCCACGGG GCCTGCCACC ATACCCACGC CGAAACAAGC GCTCATGAGC CCGAAGTGGC GAGCCCGATC TTCCCCATCG GTGATGTCGG CGATATAGGC GCCAGCAACC GCACCTGTGG CGCCGGTGAT GCCGGCCACG ATGCGTCCGG CGTAGAGGAT CGAGATCTCG ATCCCGCGAA AT