BuviA.17253.c

Phospholipase C

CENTER ID: BuviA.17253.c
ORGANISM: Burkholderia vietnamiensis G4
ASSOCIATED DISEASE:
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

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Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BuviA.17253.c.A1.GE32489 Full length( BuviA.17253.c ) 1 714
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|269482.11.peg.2409
RefSeq: YP_001116148.1
UniProt: A4JK80

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MGHCHGYFGT FKGVRGYGDR FAVPSPNGRD VFHQTYTKAT PAATITPYHL DASQGNAQRA GGTPHTWADA QAAWDHGRMN RWPDAKTPLS MGYYDAAEVP FQRALADAFT LCDHYHCGMH TGTIANRLFY WSGTNGPNGV SPADGSRVKV AALNNQFNGG NDIGPSSQGW TWTTYADRLQ HAGVSWKVYQ SLIDNFGCNE MMSFRHWRAA IEQMPAARRP AYVASTDITQ PVTAAGAFYD PTIDDALSPL AKGFGNTMPY GFLETLRDDI RTGKLPEVSW IIPPSAYSEH PGPSSPAQGA WYVQAVLDAL TANPEVWSKT VLLVNYDEND GFFDHMPSPA VPSRNADGSL AGGHTLAAAD VAVEYHDFAP ATSSQPAADG RPYGPGPRVP MWVVSPWSRG GWVNSQVFDH TSTLLFLEKR FGVVEPQISA YRRAVCGDLT SAFNFRTPND EPLPTLAGRT TKSAADALSA AQQAAPKITP PATPSLPAQA VGVRPSRALP YELHASAHVD GGNGTVTLKF ANTGRAAAVF HVYDKLHLDR VPRRYVVEPG KALRGDWAAR ADDAGKYDLW VLGPNGYHRR FTGDLAQLAG ARAPHPEIRV GYAGASGNLH LRLRNDGGDT VRFTVKSNRA YGPLSGHGAS DDHDRGADTG TTWTVAVHGG TQHELHWKLD STGHWYDFVV TADSDPSFAR RVAGRVETGR HSVSDPAMGL PERF
NT Sequence
TTGGGACATT GCCACGGTTA CTTCGGCACG TTCAAGGGCG TGCGCGGTTA CGGCGATCGC TTCGCGGTGC CGTCGCCGAA CGGCCGCGAC GTGTTCCACC AGACCTACAC GAAGGCGACG CCCGCGGCGA CCATCACGCC GTACCACCTC GATGCGAGCC AGGGCAACGC GCAGCGCGCG GGCGGCACGC CGCACACGTG GGCCGACGCG CAGGCCGCCT GGGATCACGG CCGCATGAAC CGCTGGCCCG ACGCGAAGAC GCCGCTGTCG ATGGGCTATT ACGATGCGGC CGAAGTGCCG TTCCAGCGCG CGCTCGCCGA TGCGTTCACG CTGTGCGACC ACTACCACTG CGGGATGCAC ACCGGCACGA TCGCGAACCG GCTGTTCTAC TGGAGCGGCA CCAACGGCCC GAACGGCGTC AGCCCGGCCG ACGGCAGCCG CGTGAAGGTC GCCGCACTGA ACAACCAGTT CAACGGCGGC AACGACATCG GCCCGTCGAG CCAGGGCTGG ACGTGGACGA CCTACGCGGA CCGGCTGCAG CACGCCGGCG TGAGCTGGAA GGTCTACCAG AGCCTGATCG ACAACTTCGG CTGCAACGAG ATGATGAGCT TTCGCCACTG GCGCGCGGCC ATCGAGCAGA TGCCGGCCGC ACGCCGGCCG GCGTACGTTG CGTCGACGGA CATCACGCAG CCGGTGACGG CGGCCGGCGC GTTCTACGAT CCGACGATCG ACGACGCGTT GAGCCCGCTC GCGAAAGGCT TCGGCAACAC GATGCCGTAC GGCTTTCTCG AAACGCTGCG CGACGACATC CGCACCGGCA AGCTGCCCGA AGTGTCGTGG ATCATTCCGC CGTCGGCGTA CAGCGAGCAT CCGGGGCCGT CGAGCCCCGC GCAGGGCGCG TGGTACGTGC AGGCCGTGCT CGACGCGCTG ACCGCTAACC CGGAGGTGTG GAGCAAGACG GTGCTGCTCG TCAACTACGA CGAGAACGAC GGCTTCTTCG ACCACATGCC GTCGCCGGCC GTGCCGTCGC GCAACGCCGA CGGCTCGCTC GCCGGCGGCC ATACGCTCGC GGCAGCCGAC GTCGCGGTCG AATACCACGA CTTCGCACCG GCCACCTCGA GCCAGCCCGC GGCCGACGGC CGCCCGTACG GCCCCGGCCC GCGCGTGCCG ATGTGGGTCG TGTCGCCGTG GAGTCGCGGC GGCTGGGTCA ACTCGCAGGT GTTCGACCAT ACGTCGACGC TGCTTTTCCT CGAAAAACGC TTCGGCGTGG TCGAGCCGCA GATCAGCGCC TATCGCCGCG CGGTGTGCGG CGACCTGACC AGCGCGTTCA ACTTCCGCAC GCCGAACGAC GAACCGCTGC CGACGCTCGC GGGCCGCACG ACGAAAAGCG CCGCCGATGC GCTGAGCGCA GCCCAACAGG CCGCGCCGAA GATCACGCCG CCCGCCACGC CGTCGCTGCC GGCGCAGGCC GTGGGCGTGC GTCCGTCGCG CGCGTTGCCG TACGAGCTGC ATGCGAGCGC GCACGTCGAT GGCGGCAACG GCACCGTCAC GCTGAAGTTC GCGAATACCG GCCGGGCGGC GGCGGTGTTC CATGTCTACG ACAAGCTGCA CCTCGACCGC GTGCCGCGCC GCTACGTCGT CGAGCCGGGC AAGGCGCTGC GCGGCGACTG GGCCGCACGC GCCGACGACG CCGGTAAATA TGACCTCTGG GTGCTCGGCC CGAACGGCTA TCACCGCCGC TTCACCGGCG ATCTCGCGCA GCTTGCCGGC GCACGCGCGC CGCACCCGGA GATCCGGGTC GGCTACGCGG GCGCGAGCGG CAACCTGCAT CTGCGGCTGC GCAACGACGG CGGCGACACG GTGCGCTTCA CGGTGAAATC GAACCGGGCG TACGGCCCGC TGTCCGGCCA CGGCGCCAGC GACGACCACG ACCGCGGCGC CGACACGGGG ACGACGTGGA CGGTGGCGGT GCATGGCGGC ACGCAGCACG AACTGCACTG GAAGCTCGAC TCGACCGGCC ACTGGTACGA CTTCGTCGTC ACCGCCGACA GCGACCCGAG CTTCGCGCGC CGCGTGGCCG GCCGCGTCGA GACGGGCCGT CACTCGGTCA GCGATCCGGC GATGGGGCTG CCCGAGCGCT TC
Details for BuviA.17253.c.A1.GE32489
HARVESTED ON: 11/8/2011
SEQUENCED ON: 11/11/2011
EXPECTED MW: 79kDa
OBSERVED MW: 79kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 95
PERCENT COVERAGE: 45
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMLGHCHGYF GTFKGVRGYG DRFAVPSPNG RDVFHQTYTK ATPAATITPY HLDASQGNAQ RAGGTPHTWA DAQAAWDHGR MNRWPDAKTP LSMGYYDAAE VPFQRALADA FTLCDHYHCG MHTGTIANRL FYWSGTNGPN GVSPADGSRV KVAALNNQFN GGNDIGPSSQ GWTWTTYADR LQHAGVSWKV YQSLIDNFGC NEMMSFRHWR AAIEQMPAAR RPAYVASTDI TQPVTAAGAF YDPTIDDALS PLAKGFXNTM PYGFLETLRD DXRTGKLPEV SXXIPPSAYS EHPGPSXPRX XXXXAGRARR ADX
Validated NT Sequence
ttttntttaa ctttaagaag gagatatacc atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgttgg gacattgcca cggttacttc ggcacgttca agggcgtgcg cggttacggc gatcgcttcg cggtgccgtc gccgaacggc cgcgacgtgt tccaccagac ctacacgaag gcgacgcccg cggcgaccat cacgccgtac cacctcgatg cgagccaggg caacgcgcag cgcgcgggcg gcacgccgca cacgtgggcc gacgcgcagg ccgcctggga tcacggccgc atgaaccgct ggcccgacgc gaagacgccg ctgtcgatgg gctattacga tgcggccgaa gtgccgttcc agcgcgcgct cgccgatgcg ttcacgctgt gcgaccacta ccactgcggg atgcacaccg gcacgatcgc gaaccggctg ttctactgga gcggcaccaa cggcccgaac ggcgtcagcc cggccgacgg cagccgcgtg aaggtcgccg cactgaacaa ccagttcaac ggcggcaacg acatcggccc gtcgagccag ggctggacgt ggacgaccta cgcggaccgg ctgcagcacg ccggcgtgag ctggaaggtc taccagagcc tgatcgacaa cttcggctgc aacgagatga tgagctttcg ccactggcgc gcggccatcg agcagatgcc ggccgcacgc cggccggcgt acgttgcgtc gacggacatc acgcagccgg tgacggcggc cggcgcgttc tacgatccga cgatcgacga cgcgttgagc ccgctcgcga aaggcttcng caacacgatg ccgtacggct ttctcgaaac gctgcgcgac gacntccgca ccggcaagct gcccgaagtg tcgnggntca ttccgccgtc ngcgtacagc gagcatccgg ggccgtcnan nccgcgcnng ngcgnngnac ntgcnggccg tgctcgacgc gctgacngct aaccnnnagn nnngngcana nnnnnctgct cnnnantacn acnaangacg gnttnntcga cnnntncnnc ncnnnnnnnn cnnnnnnnnn annnnnnnnn nngnnnncnn tnnnngnnnc nanntnnngn nnannnnnnn ncnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnngn nncngnnnnn nnnnnnnnnc c
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMLGHCHGYF GTFKGVRGYG DRFAVPSPNG RDVFHQTYTK ATPAATITPY HLDASQGNAQ RAGGTPHTWA DAQAAWDHGR MNRWPDAKTP LSMGYYDAAE VPFQRALADA FTLCDHYHCG MHTGTIANRL FYWSGTNGPN GVSPADGSRV KVAALNNQFN GGNDIGPSSQ GWTWTTYADR LQHAGVSWKV YQSLIDNFGC NEMMSFRHWR AAIEQMPAAR RPAYVASTDI TQPVTAAGAF YDPTIDDALS PLAKGFGNTM PYGFLETLRD DIRTGKLPEV SWIIPPSAYS EHPGPSSPAQ GAWYVQAVLD ALTANPEVWS KTVLLVNYDE NDGFFDHMPS PAVPSRNADG SLAGGHTLAA ADVAVEYHDF APATSSQPAA DGRPYGPGPR VPMWVVSPWS RGGWVNSQVF DHTSTLLFLE KRFGVVEPQI SAYRRAVCGD LTSAFNFRTP NDEPLPTLAG RTTKSAADAL SAAQQAAPKI TPPATPSLPA QAVGVRPSRA LPYELHASAH VDGGNGTVTL KFANTGRAAA VFHVYDKLHL DRVPRRYVVE PGKALRGDWA ARADDAGKYD LWVLGPNGYH RRFTGDLAQL AGARAPHPEI RVGYAGASGN LHLRLRNDGG DTVRFTVKSN RAYGPLSGHG ASDDHDRGAD TGTTWTVAVH GGTQHELHWK LDSTGHWYDF VVTADSDPSF ARRVAGRVET GRHSVSDPAM GLPERF
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gttgggacat tgccacggtt acttcggcac gttcaagggc gtgcgcggtt acggcgatcg cttcgcggtg ccgtcgccga acggccgcga cgtgttccac cagacctaca cgaaggcgac gcccgcggcg accatcacgc cgtaccacct cgatgcgagc cagggcaacg cgcagcgcgc gggcggcacg ccgcacacgt gggccgacgc gcaggccgcc tgggatcacg gccgcatgaa ccgctggccc gacgcgaaga cgccgctgtc gatgggctat tacgatgcgg ccgaagtgcc gttccagcgc gcgctcgccg atgcgttcac gctgtgcgac cactaccact gcgggatgca caccggcacg atcgcgaacc ggctgttcta ctggagcggc accaacggcc cgaacggcgt cagcccggcc gacggcagcc gcgtgaaggt cgccgcactg aacaaccagt tcaacggcgg caacgacatc ggcccgtcga gccagggctg gacgtggacg acctacgcgg accggctgca gcacgccggc gtgagctgga aggtctacca gagcctgatc gacaacttcg gctgcaacga gatgatgagc tttcgccact ggcgcgcggc catcgagcag atgccggccg cacgccggcc ggcgtacgtt gcgtcgacgg acatcacgca gccggtgacg gcggccggcg cgttctacga tccgacgatc gacgacgcgt tgagcccgct cgcgaaaggc ttcggcaaca cgatgccgta cggctttctc gaaacgctgc gcgacgacat ccgcaccggc aagctgcccg aagtgtcgtg gatcattccg ccgtcggcgt acagcgagca tccggggccg tcgagccccg cgcagggcgc gtggtacgtg caggccgtgc tcgacgcgct gaccgctaac ccggaggtgt ggagcaagac ggtgctgctc gtcaactacg acgagaacga cggcttcttc gaccacatgc cgtcgccggc cgtgccgtcg cgcaacgccg acggctcgct cgccggcggc catacgctcg cggcagccga cgtcgcggtc gaataccacg acttcgcacc ggccacctcg agccagcccg cggccgacgg ccgcccgtac ggccccggcc cgcgcgtgcc gatgtgggtc gtgtcgccgt ggagtcgcgg cggctgggtc aactcgcagg tgttcgacca tacgtcgacg ctgcttttcc tcgaaaaacg cttcggcgtg gtcgagccgc agatcagcgc ctatcgccgc gcggtgtgcg gcgacctgac cagcgcgttc aacttccgca cgccgaacga cgaaccgctg ccgacgctcg cgggccgcac gacgaaaagc gccgccgatg cgctgagcgc agcccaacag gccgcgccga agatcacgcc gcccgccacg ccgtcgctgc cggcgcaggc cgtgggcgtg cgtccgtcgc gcgcgttgcc gtacgagctg catgcgagcg cgcacgtcga tggcggcaac ggcaccgtca cgctgaagtt cgcgaatacc ggccgggcgg cggcggtgtt ccatgtctac gacaagctgc acctcgaccg cgtgccgcgc cgctacgtcg tcgagccggg caaggcgctg cgcggcgact gggccgcacg cgccgacgac gccggtaaat atgacctctg ggtgctcggc ccgaacggct atcaccgccg cttcaccggc gatctcgcgc agcttgccgg cgcacgcgcg ccgcacccgg agatccgggt cggctacgcg ggcgcgagcg gcaacctgca tctgcggctg cgcaacgacg gcggcgacac ggtgcgcttc acggtgaaat cgaaccgggc gtacggcccg ctgtccggcc acggcgccag cgacgaccac gaccgcggcg ccgacacggg gacgacgtgg acggtggcgg tgcatggcgg cacgcagcac gaactgcact ggaagctcga ctcgaccggc cactggtacg acttcgtcgt caccgccgac agcgacccga gcttcgcgcg ccgcgtggcc ggccgcgtcg agacgggccg tcactcggtc agcgatccgg cgatggggct gcccgagcgc ttctaaacag cacgaacaag ttctgcagcc aagcttctcg aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaa