BuviA.17253.a

Phospholipase C

CENTER ID: BuviA.17253.a
ORGANISM: Burkholderia vietnamiensis G4
ASSOCIATED DISEASE:
CURRENT STATUS: purified
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BuviA.17253.a.A2.GE34880 Mature protein( BuviA.17253.a ) 35 706
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BuviA.17253.a.A2.PS01560 Mature protein( BuviA.17253.a ) 35 706
BuviA.17253.a.A2.PS01563 Mature protein( BuviA.17253.a ) 35 706

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|269482.11.peg.7316
RefSeq: YP_001120870.1
UniProt: A4JID2

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MTSSSRRRFL HTVAQSAGAA VALNAFPESI RRALAIPAAR GTGTIRDVQH IVVFMQENRS FDHYFGHLRG VRGYNDRFPI PLPNGKPVWY QPSKEDPGKP VLPFRLDTKT TSAQCLGALD HSWAKTHAAI DGGRYDQWPA NKTDMTMGYH VRDDIPFHYA LADAFTVCDN YFCSLPGPTH PNRSYLMTGT IDPTGKFGGP LLDNADYVDG DLPPAYQLLS WTTFPERLEA RGVSWQIYQQ GLTWADPYNG NYGTNILQNF ANFIDAKPGS SLYQRAQTVR TLDNLKDDVI NDRLPQVSWL LPPAAFSEHP KYTPAYGANY TSQILDALTS NPDVWAKTVL FIMYDENDGF FDHVVPPQPP TSAAQGASTV TTDGELHTVV NPGRGGSYTA DGLPYGLGPR VPMTVVSPWS KGGFVCSQVF DHTSVIRFIG ERFGVDEPNI TPWRRTVCGD LTAAFDFRSS DATFPALPDT SQYRSIADQQ CTSQPAPTVP ATPSPIDPQE PGVRPARALP YELHVNASIY GGNTLRIEFA NRGNQGAHFH VYATNRTDGP WRYTVGARRV LHADFDLTET NGAYAFSVYG PNGFVRVFAG DVSAATARRR NHAQPEVKAG YDVANGNLYL KLRNHGSGHV TLTLTDNAYG APSRQVTLHG GDERVEQWAL AASHHWYDVT VSDGANPTFS RRFAGHVENG RPSYSDPAAV KPVTVS
NT Sequence
ATGACGTCAT CCAGCCGCCG CCGTTTCCTG CATACCGTCG CGCAATCGGC GGGCGCCGCC GTCGCGCTCA ACGCCTTCCC CGAATCGATC CGCCGCGCGC TCGCGATTCC CGCCGCGCGC GGCACCGGCA CGATCCGCGA TGTCCAGCAC ATCGTCGTGT TCATGCAGGA GAACCGCTCG TTCGACCATT ACTTCGGACA CCTGCGCGGC GTGCGCGGCT ACAACGACCG CTTCCCGATC CCGCTGCCGA ACGGCAAGCC GGTGTGGTAC CAGCCGTCGA AGGAGGATCC GGGCAAGCCC GTGCTGCCGT TCCGGCTCGA CACGAAGACG ACCAGCGCGC AATGTCTCGG CGCGCTCGAC CACTCGTGGG CGAAGACGCA CGCGGCGATC GACGGCGGCC GCTACGACCA GTGGCCCGCG AACAAGACCG ACATGACGAT GGGCTATCAC GTCCGCGACG ACATCCCGTT CCACTACGCG CTCGCCGATG CGTTCACCGT CTGCGACAAC TATTTCTGCT CGCTGCCGGG GCCGACGCAC CCGAACCGCT CGTATCTGAT GACCGGCACG ATCGACCCGA CCGGCAAGTT CGGCGGCCCG CTGCTCGACA ACGCCGACTA CGTCGACGGC GACCTGCCGC CCGCCTACCA GCTGCTGTCG TGGACGACCT TCCCGGAACG GCTCGAGGCG CGCGGCGTGT CGTGGCAGAT CTACCAGCAG GGGCTCACGT GGGCCGATCC GTACAACGGC AACTACGGCA CCAACATCCT GCAGAACTTC GCGAACTTCA TCGACGCGAA GCCGGGCTCG TCGCTGTATC AGCGCGCGCA GACGGTGCGC ACGCTCGACA ACCTGAAGGA CGACGTGATC AACGACCGGC TGCCGCAGGT GTCGTGGCTG CTGCCGCCGG CCGCGTTCTC CGAGCATCCG AAATACACGC CCGCGTACGG CGCGAACTAC ACGTCGCAGA TCCTCGACGC GCTGACGTCG AACCCCGACG TGTGGGCGAA GACGGTGCTG TTCATCATGT ACGACGAGAA CGACGGCTTC TTCGATCACG TCGTGCCGCC GCAGCCGCCG ACCTCGGCCG CGCAGGGCGC CTCGACGGTG ACCACCGACG GCGAGCTGCA CACGGTCGTG AACCCCGGCC GCGGCGGCAG CTATACGGCC GACGGGCTGC CGTACGGCCT CGGGCCGCGC GTGCCGATGA CGGTCGTGTC GCCGTGGTCG AAGGGCGGCT TCGTGTGCTC GCAGGTGTTC GACCACACGT CGGTGATCCG CTTCATCGGC GAACGCTTCG GCGTCGACGA GCCGAACATC ACGCCGTGGC GCCGCACCGT GTGCGGCGAC CTCACCGCCG CGTTCGACTT CCGCTCGTCG GACGCGACCT TCCCGGCGCT GCCCGACACG AGCCAGTACC GCTCGATCGC CGATCAGCAA TGCACGTCGC AGCCCGCGCC CACGGTGCCC GCGACGCCGT CGCCGATCGA TCCGCAGGAG CCGGGCGTGC GGCCGGCGCG CGCGCTGCCG TACGAGCTGC ACGTGAACGC GAGCATTTAC GGCGGCAACA CGCTGCGCAT CGAGTTCGCG AACCGCGGCA ACCAGGGCGC GCACTTCCAC GTGTACGCGA CGAACCGCAC CGACGGCCCG TGGCGCTACA CGGTCGGCGC GCGCCGCGTG CTGCACGCGG ACTTCGATCT GACCGAGACG AACGGCGCGT ATGCGTTCTC GGTGTACGGC CCGAACGGGT TCGTGCGGGT CTTCGCGGGC GACGTGTCGG CCGCGACGGC GCGCCGCCGC AACCATGCGC AGCCCGAGGT GAAGGCCGGC TACGACGTCG CGAACGGCAA TCTGTATCTG AAGCTGCGCA ATCACGGCAG CGGCCACGTG ACGTTGACGC TGACCGACAA CGCGTACGGC GCGCCGTCGC GACAGGTCAC GCTGCACGGC GGCGACGAGC GCGTCGAGCA ATGGGCGCTC GCGGCGAGCC ATCATTGGTA TGACGTGACG GTGAGCGACG GCGCGAACCC CACGTTCTCG CGGCGCTTCG CGGGCCACGT CGAGAACGGC CGGCCGAGCT ATTCGGACCC GGCGGCGGTG AAGCCCGTCA CGGTGTCG
Details for BuviA.17253.a.A2.GE34880
HARVESTED ON: 4/17/2012
SEQUENCED ON: 4/17/2012
EXPECTED MW: 76kDa
OBSERVED MW: 72kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 84
PERCENT COVERAGE: 55
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMAIPAARGT GTIRDVQHIV VFMQENRSFD HYFGHLRGVR GYNDRFPIPL PNGKPVWYQP SKEDPGKPVL PFRLDTKTTS AQCLGALDHS WAKTHAAIDG GRYDQWPANK TDMTMGYHVR DDIPFHYALA DAFTVCDNYF CSLPGPTHPN RSYLMTGTID PTGKFGGPLL DNADYVDGDL PPAYQLLSWT TFPERLEARG VSWQIYQQGL TWADPYNGNY GTNILQNFAN FIDAKPGSSL YQRAQTVRTL DNLKDDVIND RLPXVSWXLP PAAFSEHPKY TPAXGANYXS QILDALTSNP DVWAXXXLFI MYXXXXXXXS XXXXXXXXXX XXXXGXXXXX XXXGXXXXXX XXXXXXXXXX XXXXXXXX
Validated NT Sequence
ttnnttaact ttaagaagga gatataccat ggctcatcac catcaccatc atatgggtac cctggaagct cagacccagg gtcctggttc gatggcgatt cccgccgcgc gcggcaccgg cacgatccgc gatgtccagc acatcgtcgt gttcatgcag gagaaccgct cgttcgacca ttacttcgga cacctgcgcg gcgtgcgcgg ctacaacgac cgcttcccga tcccgctgcc gaacggcaag ccggtgtggt accagccgtc gaaggaggat ccgggcaagc ccgtgctgcc gttccggctc gacacgaaga cgaccagcgc gcaatgtctc ggcgcgctcg accactcgtg ggcgaagacg cacgcggcga tcgacggcgg ccgctacgac cagtggcccg cgaacaagac cgacatgacg atgggctatc acgtccgcga cgacatcccg ttccactacg cgctcgccga tgcgttcacc gtctgcgaca actatttctg ctcgctgccg gggccgacgc acccgaaccg ctcgtatctg atgaccggca cgatcgaccc gaccggcaag ttcggcggcc cgctgctcga caacgccgac tacgtcgacg gcgacctgcc gcccgcctac cagctgctgt cgtggacgac cttcccggaa cggctcgagg cgcgcggcgt gtcgtggcag atctaccagc aggggctcac gtgggccgat ccgtacaacg gcaactacgg caccaacatc ctgcagaact tcgcgaactt catcgacgcg aagccgggct cgtcgctgta tcagcgcgcg cagacggtgc gcacgctcga caacctgaag gacgacgtga tcaacgaccg gctgccgcnn gtgtcgtggn tgctgccgcc ggccgcgttc tccgagcatc cnaaatacac gcccgcgtan ggcgcgaact acncgtcgca gatcctcgac gcgctgacgt cgaaccccga cgtgtgggcn aanangnngc tgttcatcat gtacnacann nnanggntnc ntnnatcang tcnngnnnnc ncancnnnnc nnnncnnnng gngncnnnnn ggnnncnnnc nnngnnnnnn nnnnnnnggn nncnnnnnnn gnnngnngcn nnnnnnnnnn nnnnnnncnn nnnnnnnnnn nnannnnnnn nnnnncnnnn nna
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMAIPAARGT GTIRDVQHIV VFMQENRSFD HYFGHLRGVR GYNDRFPIPL PNGKPVWYQP SKEDPGKPVL PFRLDTKTTS AQCLGALDHS WAKTHAAIDG GRYDQWPANK TDMTMGYHVR DDIPFHYALA DAFTVCDNYF CSLPGPTHPN RSYLMTGTID PTGKFGGPLL DNADYVDGDL PPAYQLLSWT TFPERLEARG VSWQIYQQGL TWADPYNGNY GTNILQNFAN FIDAKPGSSL YQRAQTVRTL DNLKDDVIND RLPQVSWLLP PAAFSEHPKY TPAYGANYTS QILDALTSNP DVWAKTVLFI MYDENDGFFD HVVPPQPPTS AAQGASTVTT DGELHTVVNP GRGGSYTADG LPYGLGPRVP MTVVSPWSKG GFVCSQVFDH TSVIRFIGER FGVDEPNITP WRRTVCGDLT AAFDFRSSDA TFPALPDTSQ YRSIADQQCT SQPAPTVPAT PSPIDPQEPG VRPARALPYE LHVNASIYGG NTLRIEFANR GNQGAHFHVY ATNRTDGPWR YTVGARRVLH ADFDLTETNG AYAFSVYGPN GFVRVFAGDV SAATARRRNH AQPEVKAGYD VANGNLYLKL RNHGSGHVTL TLTDNAYGAP SRQVTLHGGD ERVEQWALAA SHHWYDVTVS DGANPTFSRR FAGHVENGRP SYSDPAAVKP VTVS
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggcgattccc gccgcgcgcg gcaccggcac gatccgcgat gtccagcaca tcgtcgtgtt catgcaggag aaccgctcgt tcgaccatta cttcggacac ctgcgcggcg tgcgcggcta caacgaccgc ttcccgatcc cgctgccgaa cggcaagccg gtgtggtacc agccgtcgaa ggaggatccg ggcaagcccg tgctgccgtt ccggctcgac acgaagacga ccagcgcgca atgtctcggc gcgctcgacc actcgtgggc gaagacgcac gcggcgatcg acggcggccg ctacgaccag tggcccgcga acaagaccga catgacgatg ggctatcacg tccgcgacga catcccgttc cactacgcgc tcgccgatgc gttcaccgtc tgcgacaact atttctgctc gctgccgggg ccgacgcacc cgaaccgctc gtatctgatg accggcacga tcgacccgac cggcaagttc ggcggcccgc tgctcgacaa cgccgactac gtcgacggcg acctgccgcc cgcctaccag ctgctgtcgt ggacgacctt cccggaacgg ctcgaggcgc gcggcgtgtc gtggcagatc taccagcagg ggctcacgtg ggccgatccg tacaacggca actacggcac caacatcctg cagaacttcg cgaacttcat cgacgcgaag ccgggctcgt cgctgtatca gcgcgcgcag acggtgcgca cgctcgacaa cctgaaggac gacgtgatca acgaccggct gccgcaggtg tcgtggctgc tgccgccggc cgcgttctcc gagcatccga aatacacgcc cgcgtacggc gcgaactaca cgtcgcagat cctcgacgcg ctgacgtcga accccgacgt gtgggcgaag acggtgctgt tcatcatgta cgacgagaac gacggcttct tcgatcacgt cgtgccgccg cagccgccga cctcggccgc gcagggcgcc tcgacggtga ccaccgacgg cgagctgcac acggtcgtga accccggccg cggcggcagc tatacggccg acgggctgcc gtacggcctc gggccgcgcg tgccgatgac ggtcgtgtcg ccgtggtcga agggcggctt cgtgtgctcg caggtgttcg accacacgtc ggtgatccgc ttcatcggcg aacgcttcgg cgtcgacgag ccgaacatca cgccgtggcg ccgcaccgtg tgcggcgacc tcaccgccgc gttcgacttc cgctcgtcgg acgcgacctt cccggcgctg cccgacacga gccagtaccg ctcgatcgcc gatcagcaat gcacgtcgca gcccgcgccc acggtgcccg cgacgccgtc gccgatcgat ccgcaggagc cgggcgtgcg gccggcgcgc gcgctgccgt acgagctgca cgtgaacgcg agcatttacg gcggcaacac gctgcgcatc gagttcgcga accgcggcaa ccagggcgcg cacttccacg tgtacgcgac gaaccgcacc gacggcccgt ggcgctacac ggtcggcgcg cgccgcgtgc tgcacgcgga cttcgatctg accgagacga acggcgcgta tgcgttctcg gtgtacggcc cgaacgggtt cgtgcgggtc ttcgcgggcg acgtgtcggc cgcgacggcg cgccgccgca accatgcgca gcccgaggtg aaggccggct acgacgtcgc gaacggcaat ctgtatctga agctgcgcaa tcacggcagc ggccacgtga cgttgacgct gaccgacaac gcgtacggcg cgccgtcgcg acaggtcacg ctgcacggcg gcgacgagcg cgtcgagcaa tgggcgctcg cggcgagcca tcattggtat gacgtgacgg tgagcgacgg cgcgaacccc acgttctcgc ggcgcttcgc gggccacgtc gagaacggcc ggccgagcta ttcggacccg gcggcggtga agcccgtcac ggtgtcgtaa acagcacgaa caagttctgc agccaagctt ctcgaggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa
Details for BuviA.17253.a.A2.PS01560
PURIFICATION DATe: 7/18/2012
CONCENTRATION: 19.9mg/ml
OBSERVED MW: 74kDa
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 2
VIAL VOLUME: 110µl
PERCENT IDENTITY: 84
PERCENT COVERAGE: 55
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMAIPAARGT GTIRDVQHIV VFMQENRSFD HYFGHLRGVR GYNDRFPIPL PNGKPVWYQP SKEDPGKPVL PFRLDTKTTS AQCLGALDHS WAKTHAAIDG GRYDQWPANK TDMTMGYHVR DDIPFHYALA DAFTVCDNYF CSLPGPTHPN RSYLMTGTID PTGKFGGPLL DNADYVDGDL PPAYQLLSWT TFPERLEARG VSWQIYQQGL TWADPYNGNY GTNILQNFAN FIDAKPGSSL YQRAQTVRTL DNLKDDVIND RLPXVSWXLP PAAFSEHPKY TPAXGANYXS QILDALTSNP DVWAXXXLFI MYXXXXXXXS XXXXXXXXXX XXXXGXXXXX XXXGXXXXXX XXXXXXXXXX XXXXXXXX
Validated NT Sequence
ttnnttaact ttaagaagga gatataccat ggctcatcac catcaccatc atatgggtac cctggaagct cagacccagg gtcctggttc gatggcgatt cccgccgcgc gcggcaccgg cacgatccgc gatgtccagc acatcgtcgt gttcatgcag gagaaccgct cgttcgacca ttacttcgga cacctgcgcg gcgtgcgcgg ctacaacgac cgcttcccga tcccgctgcc gaacggcaag ccggtgtggt accagccgtc gaaggaggat ccgggcaagc ccgtgctgcc gttccggctc gacacgaaga cgaccagcgc gcaatgtctc ggcgcgctcg accactcgtg ggcgaagacg cacgcggcga tcgacggcgg ccgctacgac cagtggcccg cgaacaagac cgacatgacg atgggctatc acgtccgcga cgacatcccg ttccactacg cgctcgccga tgcgttcacc gtctgcgaca actatttctg ctcgctgccg gggccgacgc acccgaaccg ctcgtatctg atgaccggca cgatcgaccc gaccggcaag ttcggcggcc cgctgctcga caacgccgac tacgtcgacg gcgacctgcc gcccgcctac cagctgctgt cgtggacgac cttcccggaa cggctcgagg cgcgcggcgt gtcgtggcag atctaccagc aggggctcac gtgggccgat ccgtacaacg gcaactacgg caccaacatc ctgcagaact tcgcgaactt catcgacgcg aagccgggct cgtcgctgta tcagcgcgcg cagacggtgc gcacgctcga caacctgaag gacgacgtga tcaacgaccg gctgccgcnn gtgtcgtggn tgctgccgcc ggccgcgttc tccgagcatc cnaaatacac gcccgcgtan ggcgcgaact acncgtcgca gatcctcgac gcgctgacgt cgaaccccga cgtgtgggcn aanangnngc tgttcatcat gtacnacann nnanggntnc ntnnatcang tcnngnnnnc ncancnnnnc nnnncnnnng gngncnnnnn ggnnncnnnc nnngnnnnnn nnnnnnnggn nncnnnnnnn gnnngnngcn nnnnnnnnnn nnnnnnncnn nnnnnnnnnn nnannnnnnn nnnnncnnnn nna
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMAIPAARGT GTIRDVQHIV VFMQENRSFD HYFGHLRGVR GYNDRFPIPL PNGKPVWYQP SKEDPGKPVL PFRLDTKTTS AQCLGALDHS WAKTHAAIDG GRYDQWPANK TDMTMGYHVR DDIPFHYALA DAFTVCDNYF CSLPGPTHPN RSYLMTGTID PTGKFGGPLL DNADYVDGDL PPAYQLLSWT TFPERLEARG VSWQIYQQGL TWADPYNGNY GTNILQNFAN FIDAKPGSSL YQRAQTVRTL DNLKDDVIND RLPQVSWLLP PAAFSEHPKY TPAYGANYTS QILDALTSNP DVWAKTVLFI MYDENDGFFD HVVPPQPPTS AAQGASTVTT DGELHTVVNP GRGGSYTADG LPYGLGPRVP MTVVSPWSKG GFVCSQVFDH TSVIRFIGER FGVDEPNITP WRRTVCGDLT AAFDFRSSDA TFPALPDTSQ YRSIADQQCT SQPAPTVPAT PSPIDPQEPG VRPARALPYE LHVNASIYGG NTLRIEFANR GNQGAHFHVY ATNRTDGPWR YTVGARRVLH ADFDLTETNG AYAFSVYGPN GFVRVFAGDV SAATARRRNH AQPEVKAGYD VANGNLYLKL RNHGSGHVTL TLTDNAYGAP SRQVTLHGGD ERVEQWALAA SHHWYDVTVS DGANPTFSRR FAGHVENGRP SYSDPAAVKP VTVS
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGCGATTCCC GCCGCGCGCG GCACCGGCAC GATCCGCGAT GTCCAGCACA TCGTCGTGTT CATGCAGGAG AACCGCTCGT TCGACCATTA CTTCGGACAC CTGCGCGGCG TGCGCGGCTA CAACGACCGC TTCCCGATCC CGCTGCCGAA CGGCAAGCCG GTGTGGTACC AGCCGTCGAA GGAGGATCCG GGCAAGCCCG TGCTGCCGTT CCGGCTCGAC ACGAAGACGA CCAGCGCGCA ATGTCTCGGC GCGCTCGACC ACTCGTGGGC GAAGACGCAC GCGGCGATCG ACGGCGGCCG CTACGACCAG TGGCCCGCGA ACAAGACCGA CATGACGATG GGCTATCACG TCCGCGACGA CATCCCGTTC CACTACGCGC TCGCCGATGC GTTCACCGTC TGCGACAACT ATTTCTGCTC GCTGCCGGGG CCGACGCACC CGAACCGCTC GTATCTGATG ACCGGCACGA TCGACCCGAC CGGCAAGTTC GGCGGCCCGC TGCTCGACAA CGCCGACTAC GTCGACGGCG ACCTGCCGCC CGCCTACCAG CTGCTGTCGT GGACGACCTT CCCGGAACGG CTCGAGGCGC GCGGCGTGTC GTGGCAGATC TACCAGCAGG GGCTCACGTG GGCCGATCCG TACAACGGCA ACTACGGCAC CAACATCCTG CAGAACTTCG CGAACTTCAT CGACGCGAAG CCGGGCTCGT CGCTGTATCA GCGCGCGCAG ACGGTGCGCA CGCTCGACAA CCTGAAGGAC GACGTGATCA ACGACCGGCT GCCGCAGGTG TCGTGGCTGC TGCCGCCGGC CGCGTTCTCC GAGCATCCGA AATACACGCC CGCGTACGGC GCGAACTACA CGTCGCAGAT CCTCGACGCG CTGACGTCGA ACCCCGACGT GTGGGCGAAG ACGGTGCTGT TCATCATGTA CGACGAGAAC GACGGCTTCT TCGATCACGT CGTGCCGCCG CAGCCGCCGA CCTCGGCCGC GCAGGGCGCC TCGACGGTGA CCACCGACGG CGAGCTGCAC ACGGTCGTGA ACCCCGGCCG CGGCGGCAGC TATACGGCCG ACGGGCTGCC GTACGGCCTC GGGCCGCGCG TGCCGATGAC GGTCGTGTCG CCGTGGTCGA AGGGCGGCTT CGTGTGCTCG CAGGTGTTCG ACCACACGTC GGTGATCCGC TTCATCGGCG AACGCTTCGG CGTCGACGAG CCGAACATCA CGCCGTGGCG CCGCACCGTG TGCGGCGACC TCACCGCCGC GTTCGACTTC CGCTCGTCGG ACGCGACCTT CCCGGCGCTG CCCGACACGA GCCAGTACCG CTCGATCGCC GATCAGCAAT GCACGTCGCA GCCCGCGCCC ACGGTGCCCG CGACGCCGTC GCCGATCGAT CCGCAGGAGC CGGGCGTGCG GCCGGCGCGC GCGCTGCCGT ACGAGCTGCA CGTGAACGCG AGCATTTACG GCGGCAACAC GCTGCGCATC GAGTTCGCGA ACCGCGGCAA CCAGGGCGCG CACTTCCACG TGTACGCGAC GAACCGCACC GACGGCCCGT GGCGCTACAC GGTCGGCGCG CGCCGCGTGC TGCACGCGGA CTTCGATCTG ACCGAGACGA ACGGCGCGTA TGCGTTCTCG GTGTACGGCC CGAACGGGTT CGTGCGGGTC TTCGCGGGCG ACGTGTCGGC CGCGACGGCG CGCCGCCGCA ACCATGCGCA GCCCGAGGTG AAGGCCGGCT ACGACGTCGC GAACGGCAAT CTGTATCTGA AGCTGCGCAA TCACGGCAGC GGCCACGTGA CGTTGACGCT GACCGACAAC GCGTACGGCG CGCCGTCGCG ACAGGTCACG CTGCACGGCG GCGACGAGCG CGTCGAGCAA TGGGCGCTCG CGGCGAGCCA TCATTGGTAT GACGTGACGG TGAGCGACGG CGCGAACCCC ACGTTCTCGC GGCGCTTCGC GGGCCACGTC GAGAACGGCC GGCCGAGCTA TTCGGACCCG GCGGCGGTGA AGCCCGTCAC GGTGTCGTAA ACAGCACGAA CAAGTTCTGC AGCCAAGCTT CTCGAGGATC CGGCTGCTAA CAAAGCCCGA AAGGAAGCTG AGTTGGCTGC TGCCACCGCT GAGCAATAAC TAGCATAACC CCTTGGGGCC TCTAAACGGG TCTTGAGGGG TTTTTTGCTG AAAGGAGGAA CTATATCCGG ATATCCACAG GACGGGTGTG GTCGCCATGA TCGCGTAGTC GATAGTGGCT CCAAGTAGCG AAGCGAGCAG GACTGGGCGG CGGCCAAAGC GGTCGGACAG TGCTCCGAGA ACGGGTGCGC ATAGAAATTG CATCAACGCA TATAGCGCTA GCAGCACGCC ATAGTGACTG GCGATGCTGT CGGAATGGAC GATATCCCGC AAGAGGCCCG GCAGTACCGG CATAACCAAG CCTATGCCTA CAGCATCCAG GGTGACGGTG CCGAGGATGA CGATGAGCGC ATTGTTAGAT TTCATACACG GTGCCTGACT GCGTTAGCAA TTTAACTGTG ATAAACTACC GCATTAAAGC TTATCGATGA TAAGCTGTCA AACATGAGAA
Details for BuviA.17253.a.A2.PS01563
PURIFICATION DATe: 7/18/2012
CONCENTRATION: 27.5mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 5
VIAL VOLUME: 110µl
PERCENT IDENTITY: 84
PERCENT COVERAGE: 55
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMAIPAARGT GTIRDVQHIV VFMQENRSFD HYFGHLRGVR GYNDRFPIPL PNGKPVWYQP SKEDPGKPVL PFRLDTKTTS AQCLGALDHS WAKTHAAIDG GRYDQWPANK TDMTMGYHVR DDIPFHYALA DAFTVCDNYF CSLPGPTHPN RSYLMTGTID PTGKFGGPLL DNADYVDGDL PPAYQLLSWT TFPERLEARG VSWQIYQQGL TWADPYNGNY GTNILQNFAN FIDAKPGSSL YQRAQTVRTL DNLKDDVIND RLPXVSWXLP PAAFSEHPKY TPAXGANYXS QILDALTSNP DVWAXXXLFI MYXXXXXXXS XXXXXXXXXX XXXXGXXXXX XXXGXXXXXX XXXXXXXXXX XXXXXXXX
Validated NT Sequence
ttnnttaact ttaagaagga gatataccat ggctcatcac catcaccatc atatgggtac cctggaagct cagacccagg gtcctggttc gatggcgatt cccgccgcgc gcggcaccgg cacgatccgc gatgtccagc acatcgtcgt gttcatgcag gagaaccgct cgttcgacca ttacttcgga cacctgcgcg gcgtgcgcgg ctacaacgac cgcttcccga tcccgctgcc gaacggcaag ccggtgtggt accagccgtc gaaggaggat ccgggcaagc ccgtgctgcc gttccggctc gacacgaaga cgaccagcgc gcaatgtctc ggcgcgctcg accactcgtg ggcgaagacg cacgcggcga tcgacggcgg ccgctacgac cagtggcccg cgaacaagac cgacatgacg atgggctatc acgtccgcga cgacatcccg ttccactacg cgctcgccga tgcgttcacc gtctgcgaca actatttctg ctcgctgccg gggccgacgc acccgaaccg ctcgtatctg atgaccggca cgatcgaccc gaccggcaag ttcggcggcc cgctgctcga caacgccgac tacgtcgacg gcgacctgcc gcccgcctac cagctgctgt cgtggacgac cttcccggaa cggctcgagg cgcgcggcgt gtcgtggcag atctaccagc aggggctcac gtgggccgat ccgtacaacg gcaactacgg caccaacatc ctgcagaact tcgcgaactt catcgacgcg aagccgggct cgtcgctgta tcagcgcgcg cagacggtgc gcacgctcga caacctgaag gacgacgtga tcaacgaccg gctgccgcnn gtgtcgtggn tgctgccgcc ggccgcgttc tccgagcatc cnaaatacac gcccgcgtan ggcgcgaact acncgtcgca gatcctcgac gcgctgacgt cgaaccccga cgtgtgggcn aanangnngc tgttcatcat gtacnacann nnanggntnc ntnnatcang tcnngnnnnc ncancnnnnc nnnncnnnng gngncnnnnn ggnnncnnnc nnngnnnnnn nnnnnnnggn nncnnnnnnn gnnngnngcn nnnnnnnnnn nnnnnnncnn nnnnnnnnnn nnannnnnnn nnnnncnnnn nna
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMAIPAARGT GTIRDVQHIV VFMQENRSFD HYFGHLRGVR GYNDRFPIPL PNGKPVWYQP SKEDPGKPVL PFRLDTKTTS AQCLGALDHS WAKTHAAIDG GRYDQWPANK TDMTMGYHVR DDIPFHYALA DAFTVCDNYF CSLPGPTHPN RSYLMTGTID PTGKFGGPLL DNADYVDGDL PPAYQLLSWT TFPERLEARG VSWQIYQQGL TWADPYNGNY GTNILQNFAN FIDAKPGSSL YQRAQTVRTL DNLKDDVIND RLPQVSWLLP PAAFSEHPKY TPAYGANYTS QILDALTSNP DVWAKTVLFI MYDENDGFFD HVVPPQPPTS AAQGASTVTT DGELHTVVNP GRGGSYTADG LPYGLGPRVP MTVVSPWSKG GFVCSQVFDH TSVIRFIGER FGVDEPNITP WRRTVCGDLT AAFDFRSSDA TFPALPDTSQ YRSIADQQCT SQPAPTVPAT PSPIDPQEPG VRPARALPYE LHVNASIYGG NTLRIEFANR GNQGAHFHVY ATNRTDGPWR YTVGARRVLH ADFDLTETNG AYAFSVYGPN GFVRVFAGDV SAATARRRNH AQPEVKAGYD VANGNLYLKL RNHGSGHVTL TLTDNAYGAP SRQVTLHGGD ERVEQWALAA SHHWYDVTVS DGANPTFSRR FAGHVENGRP SYSDPAAVKP VTVS
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGCGATTCCC GCCGCGCGCG GCACCGGCAC GATCCGCGAT GTCCAGCACA TCGTCGTGTT CATGCAGGAG AACCGCTCGT TCGACCATTA CTTCGGACAC CTGCGCGGCG TGCGCGGCTA CAACGACCGC TTCCCGATCC CGCTGCCGAA CGGCAAGCCG GTGTGGTACC AGCCGTCGAA GGAGGATCCG GGCAAGCCCG TGCTGCCGTT CCGGCTCGAC ACGAAGACGA CCAGCGCGCA ATGTCTCGGC GCGCTCGACC ACTCGTGGGC GAAGACGCAC GCGGCGATCG ACGGCGGCCG CTACGACCAG TGGCCCGCGA ACAAGACCGA CATGACGATG GGCTATCACG TCCGCGACGA CATCCCGTTC CACTACGCGC TCGCCGATGC GTTCACCGTC TGCGACAACT ATTTCTGCTC GCTGCCGGGG CCGACGCACC CGAACCGCTC GTATCTGATG ACCGGCACGA TCGACCCGAC CGGCAAGTTC GGCGGCCCGC TGCTCGACAA CGCCGACTAC GTCGACGGCG ACCTGCCGCC CGCCTACCAG CTGCTGTCGT GGACGACCTT CCCGGAACGG CTCGAGGCGC GCGGCGTGTC GTGGCAGATC TACCAGCAGG GGCTCACGTG GGCCGATCCG TACAACGGCA ACTACGGCAC CAACATCCTG CAGAACTTCG CGAACTTCAT CGACGCGAAG CCGGGCTCGT CGCTGTATCA GCGCGCGCAG ACGGTGCGCA CGCTCGACAA CCTGAAGGAC GACGTGATCA ACGACCGGCT GCCGCAGGTG TCGTGGCTGC TGCCGCCGGC CGCGTTCTCC GAGCATCCGA AATACACGCC CGCGTACGGC GCGAACTACA CGTCGCAGAT CCTCGACGCG CTGACGTCGA ACCCCGACGT GTGGGCGAAG ACGGTGCTGT TCATCATGTA CGACGAGAAC GACGGCTTCT TCGATCACGT CGTGCCGCCG CAGCCGCCGA CCTCGGCCGC GCAGGGCGCC TCGACGGTGA CCACCGACGG CGAGCTGCAC ACGGTCGTGA ACCCCGGCCG CGGCGGCAGC TATACGGCCG ACGGGCTGCC GTACGGCCTC GGGCCGCGCG TGCCGATGAC GGTCGTGTCG CCGTGGTCGA AGGGCGGCTT CGTGTGCTCG CAGGTGTTCG ACCACACGTC GGTGATCCGC TTCATCGGCG AACGCTTCGG CGTCGACGAG CCGAACATCA CGCCGTGGCG CCGCACCGTG TGCGGCGACC TCACCGCCGC GTTCGACTTC CGCTCGTCGG ACGCGACCTT CCCGGCGCTG CCCGACACGA GCCAGTACCG CTCGATCGCC GATCAGCAAT GCACGTCGCA GCCCGCGCCC ACGGTGCCCG CGACGCCGTC GCCGATCGAT CCGCAGGAGC CGGGCGTGCG GCCGGCGCGC GCGCTGCCGT ACGAGCTGCA CGTGAACGCG AGCATTTACG GCGGCAACAC GCTGCGCATC GAGTTCGCGA ACCGCGGCAA CCAGGGCGCG CACTTCCACG TGTACGCGAC GAACCGCACC GACGGCCCGT GGCGCTACAC GGTCGGCGCG CGCCGCGTGC TGCACGCGGA CTTCGATCTG ACCGAGACGA ACGGCGCGTA TGCGTTCTCG GTGTACGGCC CGAACGGGTT CGTGCGGGTC TTCGCGGGCG ACGTGTCGGC CGCGACGGCG CGCCGCCGCA ACCATGCGCA GCCCGAGGTG AAGGCCGGCT ACGACGTCGC GAACGGCAAT CTGTATCTGA AGCTGCGCAA TCACGGCAGC GGCCACGTGA CGTTGACGCT GACCGACAAC GCGTACGGCG CGCCGTCGCG ACAGGTCACG CTGCACGGCG GCGACGAGCG CGTCGAGCAA TGGGCGCTCG CGGCGAGCCA TCATTGGTAT GACGTGACGG TGAGCGACGG CGCGAACCCC ACGTTCTCGC GGCGCTTCGC GGGCCACGTC GAGAACGGCC GGCCGAGCTA TTCGGACCCG GCGGCGGTGA AGCCCGTCAC GGTGTCGTAA ACAGCACGAA CAAGTTCTGC AGCCAAGCTT CTCGAGGATC CGGCTGCTAA CAAAGCCCGA AAGGAAGCTG AGTTGGCTGC TGCCACCGCT GAGCAATAAC TAGCATAACC CCTTGGGGCC TCTAAACGGG TCTTGAGGGG TTTTTTGCTG AAAGGAGGAA CTATATCCGG ATATCCACAG GACGGGTGTG GTCGCCATGA TCGCGTAGTC GATAGTGGCT CCAAGTAGCG AAGCGAGCAG GACTGGGCGG CGGCCAAAGC GGTCGGACAG TGCTCCGAGA ACGGGTGCGC ATAGAAATTG CATCAACGCA TATAGCGCTA GCAGCACGCC ATAGTGACTG GCGATGCTGT CGGAATGGAC GATATCCCGC AAGAGGCCCG GCAGTACCGG CATAACCAAG CCTATGCCTA CAGCATCCAG GGTGACGGTG CCGAGGATGA CGATGAGCGC ATTGTTAGAT TTCATACACG GTGCCTGACT GCGTTAGCAA TTTAACTGTG ATAAACTACC GCATTAAAGC TTATCGATGA TAAGCTGTCA AACATGAGAA