BuviA.17230.a

1-deoxy-D-xylulose-5-phosphate synthase (DXS)

CENTER ID:	BuviA.17230.a
ORGANISM:	Burkholderia vietnamiensis G4
ASSOCIATED DISEASE:
CURRENT STATUS:	purified
COMMUNITY REQUEST:	True
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BuviA.17230.a.A1.GE32485	Full length( BuviA.17230.a )		1	646

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BuviA.17230.a.A1.PS01469	Full length( BuviA.17230.a )		1	646

External Resources

RESOURCE	REFERENCE ID
OrthoMCL:	OG5_129068
PATRIC ID:	fig\|269482.11.peg.3005
RefSeq:	YP_001116702.1
UniProt:	A4JLT4

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MRARLISYNG TTMYDLLKTI DDPADLRRLD RRQLQPLADE LRAFVLDSVS KTGGHLSSNL GTVELTIALH YVFNTPNDRI VWDVGHQTYP HKILTGRRDQ MHTLRQYDGI SGFPRRSESE YDTFGTAHSS TSISAALGMA IGSQLNGDDR FSIAVIGDGA MTAGMAFEAM NNAGVSEDAK LLVILNDNDM SISPPVGALN RHLARLMSGR FYAAARAGVE RVLSVAPPVL ELARKLEEHA KGMVVPATLF EEFGFNYIGP IDGHDLDSLI PTLQNIRELR GPQFLHVVTK KGQGYKLAEA DPVLYHGPGK FNPAEGIKPS ATPAKKTYTQ VFGEWLCDEA ERDSRVVGIT PAMREGSGMV EFEKRFKDRY YDVGIAEQHA VTFAGGLATE GLKPVVAIYS TFLQRAYDQL IHDVALQNLP VVFAIDRAGL VGADGATHAG AYDLAFMRCI PNMTIMAASD ENECRQMLHT ALQQPNPTAV RYPRGSGTGV ATVKEFTALP IGKGEVRRRS SQPEGKRVAI LAFGTMVAPS LAAADELDAT VANMRFVKPI DAALVRELAE THDYLVTVEE GCLMGGAGSA CVEALMESGV IRPVLQLGLP DQFIDHGDPA KLLSQCGLDS AGIAKSIRER FLSPVADLAG EAKRVA

NT Sequence

ATGCGGGCGC GTTTGATTTC CTACAATGGA ACGACGATGT ACGACTTGCT GAAAACCATC GACGACCCGG CGGATCTGCG CCGCCTCGAT CGTCGCCAAC TGCAACCGCT CGCGGATGAA CTGCGCGCGT TCGTGCTCGA CAGCGTGTCG AAGACGGGCG GCCATCTGTC GTCCAACCTC GGGACGGTCG AGCTGACGAT CGCGTTGCAC TACGTGTTCA ACACGCCGAA CGACCGGATC GTCTGGGACG TGGGTCATCA GACCTACCCG CACAAGATCC TGACCGGCCG CCGCGACCAG ATGCACACGC TGCGCCAGTA CGACGGCATC TCGGGCTTCC CGCGCCGCAG CGAGTCCGAG TACGACACGT TCGGCACCGC GCATTCGAGC ACGTCGATCT CGGCGGCGCT CGGGATGGCG ATCGGCAGCC AGCTGAACGG CGACGACCGC TTCTCGATCG CGGTGATCGG CGACGGCGCG ATGACGGCCG GCATGGCGTT CGAGGCGATG AACAACGCGG GCGTGTCGGA GGATGCGAAG CTGCTCGTGA TCCTCAACGA CAACGACATG TCGATTTCGC CGCCGGTCGG CGCGCTGAAC CGCCATCTCG CACGTCTGAT GTCGGGCCGC TTCTACGCGG CCGCGCGCGC GGGCGTCGAG CGCGTGCTGA GCGTGGCGCC GCCGGTGCTC GAGCTTGCAC GCAAGCTCGA GGAGCACGCG AAGGGCATGG TCGTGCCGGC CACGTTATTC GAGGAATTCG GCTTCAACTA CATCGGCCCG ATCGACGGTC ACGATCTCGA TTCGCTGATC CCGACGCTGC AGAACATCCG CGAGCTGCGC GGTCCGCAGT TCCTGCACGT GGTCACGAAG AAAGGCCAGG GCTACAAGCT CGCCGAAGCC GATCCGGTGC TCTACCACGG CCCCGGCAAG TTCAATCCGG CCGAAGGCAT CAAGCCGTCC GCCACGCCGG CGAAGAAGAC CTACACGCAG GTGTTCGGCG AATGGCTGTG CGACGAAGCC GAGCGCGATT CGCGCGTCGT CGGCATCACG CCGGCCATGC GCGAAGGCTC CGGCATGGTC GAGTTCGAGA AGCGCTTCAA GGATCGCTAC TACGACGTCG GCATCGCCGA GCAGCACGCG GTGACGTTCG CTGGCGGGCT CGCGACCGAA GGGCTCAAGC CGGTCGTCGC GATCTACTCG ACGTTCCTGC AGCGTGCGTA CGACCAGCTG ATCCACGACG TCGCGCTGCA GAACCTGCCG GTCGTGTTCG CGATCGACCG CGCGGGCCTC GTCGGCGCCG ACGGTGCGAC GCACGCGGGT GCATACGATC TCGCGTTCAT GCGCTGCATC CCGAACATGA CGATCATGGC CGCGTCCGAC GAGAACGAAT GCCGGCAGAT GCTGCACACG GCGCTTCAGC AGCCGAACCC GACCGCCGTG CGCTATCCGC GCGGCTCGGG CACCGGCGTG GCGACGGTCA AGGAGTTCAC CGCGCTTCCG ATCGGCAAGG GCGAAGTGCG CCGCCGTTCG TCGCAGCCGG AAGGCAAGCG CGTCGCGATC CTCGCGTTCG GCACGATGGT CGCGCCGTCG CTCGCGGCGG CCGACGAACT CGACGCAACG GTCGCGAACA TGCGCTTCGT GAAGCCGATC GACGCGGCGC TCGTGCGTGA GCTCGCCGAG ACGCACGACT ATCTCGTCAC GGTCGAGGAA GGCTGCCTGA TGGGTGGCGC AGGTTCCGCG TGCGTCGAAG CCCTGATGGA GAGTGGGGTT ATCCGACCCG TACTACAATT GGGCCTCCCT GACCAGTTCA TCGATCACGG CGACCCCGCG AAGCTGCTGT CGCAATGCGG CCTCGACAGC GCGGGCATCG CGAAGTCGAT TCGCGAACGC TTCCTCAGCC CGGTAGCCGA TCTCGCCGGC GAGGCGAAGC GCGTCGCA

Details for BuviA.17230.a.A1.GE32485

HARVESTED ON:	11/8/2011
SEQUENCED ON:	5/1/2015
EXPECTED MW:	72kDa
OBSERVED MW:	72kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Few (1-10)
TOTAL EXPRESSION LEVEL:	High Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	97
PERCENT COVERAGE:	47

Validated AA Sequence

MGTLEAQTQG PGSMRARLIS YNGTTMYDLL KTIDDPADLR RLDRRQLQPL ADELRAFVLD SVSKTGGHLS SNLGTVELTI ALHYVFNTPN DRIVWDVGHQ TYPHKILTGR RDQMHTLRQY DGISGFPRRS ESEYDTFGTA HSSTSISAAL GMAIGSQLNG DDRFSIAVIG DGAMTAGMAF EAMNNAGVSE DAKLLVILND NDMSISPPVG ALNRHLARLM SGRFYAAARA GVERVLSVAP PVLELARKLE EHAKGMVVPA TLFEEFGFNY IGPIDGHDLD SLIPTLQNIR ELRGPQFLHV VXXKGQGYKL XXXXPXL

Validated NT Sequence

ggctcatcac catcaccatc atatgggtac cctggaagct cagacccagg gtcctggttc gatgcgggcg cgtttgattt cctacaatgg aacgacgatg tacgacttgc tgaaaaccat cgacgacccg gcggatctgc gccgcctcga tcgtcgccaa ctgcaaccgc tcgcggatga actgcgcgcg ttcgtgctcg acagcgtgtc gaagacgggc ggccatctgt cgtccaacct cgggacggtc gagctgacga tcgcgttgca ctacgtgttc aacacgccga acgaccggat cgtctgggac gtgggtcatc agacctaccc gcacaagatc ctgaccggcc gccgcgacca gatgcacacg ctgcgccagt acgacggcat ctcgggcttc ccgcgccgca gcgagtccga gtacgacacg ttcggcaccg cgcattcgag cacgtcgatc tcggcggcgc tcgggatggc gatcggcagc cagctgaacg gcgacgaccg cttctcgatc gcggtgatcg gcgacggcgc gatgacggcc ggcatggcgt tcgaggcgat gaacaacgcg ggcgtgtcgg aggatgcgaa gctgctcgtg atcctcaacg acaacgacat gtcgatttcg ccgccggtcg gcgcgctgaa ccgccatctc gcacgtctga tgtcgggccg cttctacgcg gccgcgcgcg cgggcgtcga gcgcgtgctg agcgtggcgc cgccggtgct cgagcttgca cgcaagctcg aggagcacgc gaagggcatg gtcgtgccgg ccacgttatt cgaggaattc ggcttcaact acatcggccc gatcgacggt cacgatctcg attcgctgat cccgacgctg cagaacatcc gcgagctgcg cggtccgcag ttcctgcacg tggtnncnaa naaaggncag ggctacaagc tcgnnnnnnn nnnnccgnng ctct

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMRARLISYN GTTMYDLLKT IDDPADLRRL DRRQLQPLAD ELRAFVLDSV SKTGGHLSSN LGTVELTIAL HYVFNTPNDR IVWDVGHQTY PHKILTGRRD QMHTLRQYDG ISGFPRRSES EYDTFGTAHS STSISAALGM AIGSQLNGDD RFSIAVIGDG AMTAGMAFEA MNNAGVSEDA KLLVILNDND MSISPPVGAL NRHLARLMSG RFYAAARAGV ERVLSVAPPV LELARKLEEH AKGMVVPATL FEEFGFNYIG PIDGHDLDSL IPTLQNIREL RGPQFLHVVT KKGQGYKLAE ADPVLYHGPG KFNPAEGIKP SATPAKKTYT QVFGEWLCDE AERDSRVVGI TPAMREGSGM VEFEKRFKDR YYDVGIAEQH AVTFAGGLAT EGLKPVVAIY STFLQRAYDQ LIHDVALQNL PVVFAIDRAG LVGADGATHA GAYDLAFMRC IPNMTIMAAS DENECRQMLH TALQQPNPTA VRYPRGSGTG VATVKEFTAL PIGKGEVRRR SSQPEGKRVA ILAFGTMVAP SLAAADELDA TVANMRFVKP IDAALVRELA ETHDYLVTVE EGCLMGGAGS ACVEALMESG VIRPVLQLGL PDQFIDHGDP AKLLSQCGLD SAGIAKSIRE RFLSPVADLA GEAKRVA

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gcgggcgcgt ttgatttcct acaatggaac gacgatgtac gacttgctga aaaccatcga cgacccggcg gatctgcgcc gcctcgatcg tcgccaactg caaccgctcg cggatgaact gcgcgcgttc gtgctcgaca gcgtgtcgaa gacgggcggc catctgtcgt ccaacctcgg gacggtcgag ctgacgatcg cgttgcacta cgtgttcaac acgccgaacg accggatcgt ctgggacgtg ggtcatcaga cctacccgca caagatcctg accggccgcc gcgaccagat gcacacgctg cgccagtacg acggcatctc gggcttcccg cgccgcagcg agtccgagta cgacacgttc ggcaccgcgc attcgagcac gtcgatctcg gcggcgctcg ggatggcgat cggcagccag ctgaacggcg acgaccgctt ctcgatcgcg gtgatcggcg acggcgcgat gacggccggc atggcgttcg aggcgatgaa caacgcgggc gtgtcggagg atgcgaagct gctcgtgatc ctcaacgaca acgacatgtc gatttcgccg ccggtcggcg cgctgaaccg ccatctcgca cgtctgatgt cgggccgctt ctacgcggcc gcgcgcgcgg gcgtcgagcg cgtgctgagc gtggcgccgc cggtgctcga gcttgcacgc aagctcgagg agcacgcgaa gggcatggtc gtgccggcca cgttattcga ggaattcggc ttcaactaca tcggcccgat cgacggtcac gatctcgatt cgctgatccc gacgctgcag aacatccgcg agctgcgcgg tccgcagttc ctgcacgtgg tcacgaagaa aggccagggc tacaagctcg ccgaagccga tccggtgctc taccacggcc ccggcaagtt caatccggcc gaaggcatca agccgtccgc cacgccggcg aagaagacct acacgcaggt gttcggcgaa tggctgtgcg acgaagccga gcgcgattcg cgcgtcgtcg gcatcacgcc ggccatgcgc gaaggctccg gcatggtcga gttcgagaag cgcttcaagg atcgctacta cgacgtcggc atcgccgagc agcacgcggt gacgttcgct ggcgggctcg cgaccgaagg gctcaagccg gtcgtcgcga tctactcgac gttcctgcag cgtgcgtacg accagctgat ccacgacgtc gcgctgcaga acctgccggt cgtgttcgcg atcgaccgcg cgggcctcgt cggcgccgac ggtgcgacgc acgcgggtgc atacgatctc gcgttcatgc gctgcatccc gaacatgacg atcatggccg cgtccgacga gaacgaatgc cggcagatgc tgcacacggc gcttcagcag ccgaacccga ccgccgtgcg ctatccgcgc ggctcgggca ccggcgtggc gacggtcaag gagttcaccg cgcttccgat cggcaagggc gaagtgcgcc gccgttcgtc gcagccggaa ggcaagcgcg tcgcgatcct cgcgttcggc acgatggtcg cgccgtcgct cgcggcggcc gacgaactcg acgcaacggt cgcgaacatg cgcttcgtga agccgatcga cgcggcgctc gtgcgtgagc tcgccgagac gcacgactat ctcgtcacgg tcgaggaagg ctgcctgatg ggtggcgcag gttccgcgtg cgtcgaagcc ctgatggaga gtggggttat ccgacccgta ctacaattgg gcctccctga ccagttcatc gatcacggcg accccgcgaa gctgctgtcg caatgcggcc tcgacagcgc gggcatcgcg aagtcgattc gcgaacgctt cctcagcccg gtagccgatc tcgccggcga ggcgaagcgc gtcgcataaa cagcacgaac aagttctgca gccaagcttc tcgaggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaa

Details for BuviA.17230.a.A1.PS01469

PURIFICATION DATe:	4/17/2012
CONCENTRATION:	35.1mg/ml
OBSERVED MW:	73kDa
EXPRESSION LEVEL:	Low Expression
PROTEIN PURIFICATION BUFFER:	20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	7
VIAL VOLUME:	110µl
PERCENT IDENTITY:	97
PERCENT COVERAGE:	47

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GCGGGCGCGT TTGATTTCCT ACAATGGAAC GACGATGTAC GACTTGCTGA AAACCATCGA CGACCCGGCG GATCTGCGCC GCCTCGATCG TCGCCAACTG CAACCGCTCG CGGATGAACT GCGCGCGTTC GTGCTCGACA GCGTGTCGAA GACGGGCGGC CATCTGTCGT CCAACCTCGG GACGGTCGAG CTGACGATCG CGTTGCACTA CGTGTTCAAC ACGCCGAACG ACCGGATCGT CTGGGACGTG GGTCATCAGA CCTACCCGCA CAAGATCCTG ACCGGCCGCC GCGACCAGAT GCACACGCTG CGCCAGTACG ACGGCATCTC GGGCTTCCCG CGCCGCAGCG AGTCCGAGTA CGACACGTTC GGCACCGCGC ATTCGAGCAC GTCGATCTCG GCGGCGCTCG GGATGGCGAT CGGCAGCCAG CTGAACGGCG ACGACCGCTT CTCGATCGCG GTGATCGGCG ACGGCGCGAT GACGGCCGGC ATGGCGTTCG AGGCGATGAA CAACGCGGGC GTGTCGGAGG ATGCGAAGCT GCTCGTGATC CTCAACGACA ACGACATGTC GATTTCGCCG CCGGTCGGCG CGCTGAACCG CCATCTCGCA CGTCTGATGT CGGGCCGCTT CTACGCGGCC GCGCGCGCGG GCGTCGAGCG CGTGCTGAGC GTGGCGCCGC CGGTGCTCGA GCTTGCACGC AAGCTCGAGG AGCACGCGAA GGGCATGGTC GTGCCGGCCA CGTTATTCGA GGAATTCGGC TTCAACTACA TCGGCCCGAT CGACGGTCAC GATCTCGATT CGCTGATCCC GACGCTGCAG AACATCCGCG AGCTGCGCGG TCCGCAGTTC CTGCACGTGG TCACGAAGAA AGGCCAGGGC TACAAGCTCG CCGAAGCCGA TCCGGTGCTC TACCACGGCC CCGGCAAGTT CAATCCGGCC GAAGGCATCA AGCCGTCCGC CACGCCGGCG AAGAAGACCT ACACGCAGGT GTTCGGCGAA TGGCTGTGCG ACGAAGCCGA GCGCGATTCG CGCGTCGTCG GCATCACGCC GGCCATGCGC GAAGGCTCCG GCATGGTCGA GTTCGAGAAG CGCTTCAAGG ATCGCTACTA CGACGTCGGC ATCGCCGAGC AGCACGCGGT GACGTTCGCT GGCGGGCTCG CGACCGAAGG GCTCAAGCCG GTCGTCGCGA TCTACTCGAC GTTCCTGCAG CGTGCGTACG ACCAGCTGAT CCACGACGTC GCGCTGCAGA ACCTGCCGGT CGTGTTCGCG ATCGACCGCG CGGGCCTCGT CGGCGCCGAC GGTGCGACGC ACGCGGGTGC ATACGATCTC GCGTTCATGC GCTGCATCCC GAACATGACG ATCATGGCCG CGTCCGACGA GAACGAATGC CGGCAGATGC TGCACACGGC GCTTCAGCAG CCGAACCCGA CCGCCGTGCG CTATCCGCGC GGCTCGGGCA CCGGCGTGGC GACGGTCAAG GAGTTCACCG CGCTTCCGAT CGGCAAGGGC GAAGTGCGCC GCCGTTCGTC GCAGCCGGAA GGCAAGCGCG TCGCGATCCT CGCGTTCGGC ACGATGGTCG CGCCGTCGCT CGCGGCGGCC GACGAACTCG ACGCAACGGT CGCGAACATG CGCTTCGTGA AGCCGATCGA CGCGGCGCTC GTGCGTGAGC TCGCCGAGAC GCACGACTAT CTCGTCACGG TCGAGGAAGG CTGCCTGATG GGTGGCGCAG GTTCCGCGTG CGTCGAAGCC CTGATGGAGA GTGGGGTTAT CCGACCCGTA CTACAATTGG GCCTCCCTGA CCAGTTCATC GATCACGGCG ACCCCGCGAA GCTGCTGTCG CAATGCGGCC TCGACAGCGC GGGCATCGCG AAGTCGATTC GCGAACGCTT CCTCAGCCCG GTAGCCGATC TCGCCGGCGA GGCGAAGCGC GTCGCATAAA CAGCACGAAC AAGTTCTGCA GCCAAGCTTC TCGAGGATCC GGCTGCTAAC AAAGCCCGAA AGGAAGCTGA GTTGGCTGCT GCCACCGCTG AGCAATAACT AGCATAACCC CTTGGGGCCT CTAAACGGGT CTTGAGGGGT TTTTTGCTGA AAGGAGGAAC TATATCCGGA TATCCACAGG ACGGGTGTGG TCGCCATGAT CGCGTAGTCG ATAGTGGCTC CAAGTAGCGA AGCGAGCAGG ACTGGGCGGC GGCCAAAGCG GTCGGACAGT GCTCCGAGAA CGGGTGCGCA TAGAAATTGC ATCAACGCAT ATAGCGCTAG CAGCACGCCA TAGTGACTGG CGATGCTGTC GGAATGGACG ATATCCCGCA AGAGGCCCGG CAGTACCGGC ATAACCAAGC CTATGCCTAC AGCATCCAGG GTGACGGTGC CGAGGATGAC GATGAGCGCA TTGTTAGATT TCATACACGG TGCCTGACTG CGTTAGCAAT TTAACTGTGA TAAACTACCG CATTAAAGCT TATCGATGAT AAGCTGTCAA ACATGAGAA