SSGCID: Target Page

BuviA.17229.a

4-hydroxy-3-methylbut-2-enyl diphosphate reductase (IspH/LytB) (IspG/H)

CENTER ID:	BuviA.17229.a
ORGANISM:	Burkholderia vietnamiensis G4
ASSOCIATED DISEASE:
CURRENT STATUS:	crystallized
COMMUNITY REQUEST:	True
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BuviA.17229.a.A1.GE32483	Full length( BuviA.17229.a )		1	326

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BuviA.17229.a.A1.PS01328	Full length( BuviA.17229.a )		1	326

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|269482.11.peg.6832
RefSeq:	YP_001120407.1
UniProt:	A4JH19

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MSNTDTLPGQ TVAADAEILL AQPRGFCAGV DRAIEIVERA IAMHGAPIYV RHEIVHNKYV VEDLRKKGAI FVEELEEVPA GNTVIFSAHG VSKAVRDEAD VRGLRIYDAT CPLVTKVHVE VAKMRQDGVD IVMIGHKGHP EVEGTMGQVE RGMHLVESVE DVQKLELADP ERIALVTQTT LSVDDAAEII GALKAKYPKI REPKKQDICY ATQNRQDAVK FMAPQCDVVI VVGSPNSSNS SRLREVAEKR GVAAYMVDAP EQIDPAWVAG KRRIGVTAGA SAPEVLAQAV IARLRELGVR NVRALEGIEE NVAFPLPRGL NLPPAA

NT Sequence

ATGAGCAACA CCGATACGCT GCCCGGGCAG ACCGTCGCAG CCGATGCCGA AATCCTGCTG GCACAGCCGC GCGGCTTCTG CGCCGGTGTC GACCGCGCGA TCGAGATCGT CGAGCGCGCG ATCGCGATGC ACGGTGCACC GATCTACGTG CGTCACGAAA TCGTCCACAA CAAGTACGTG GTCGAGGATC TGAGGAAGAA GGGCGCGATC TTCGTCGAAG AGCTCGAGGA AGTGCCGGCC GGCAACACGG TGATCTTCAG TGCGCATGGC GTGTCGAAAG CCGTGCGCGA CGAGGCCGAC GTGCGCGGCC TGCGCATTTA CGACGCGACC TGCCCGCTCG TCACGAAAGT GCACGTCGAA GTCGCGAAGA TGCGCCAGGA CGGCGTCGAC ATCGTGATGA TCGGCCACAA GGGCCACCCG GAAGTCGAAG GCACGATGGG GCAGGTCGAG CGCGGCATGC ATCTGGTCGA AAGCGTCGAG GACGTCCAGA AGCTCGAACT CGCCGATCCC GAGCGCATCG CGCTCGTCAC GCAAACGACG CTGTCCGTTG ATGACGCAGC CGAAATCATC GGCGCGCTGA AGGCGAAGTA CCCGAAGATC CGCGAGCCGA AGAAGCAGGA CATCTGCTAT GCGACGCAGA ACCGCCAGGA CGCGGTGAAG TTCATGGCGC CGCAGTGCGA CGTCGTGATC GTGGTCGGCA GCCCGAACAG TTCGAATTCC AGCCGCCTGC GCGAAGTCGC GGAGAAGCGC GGCGTGGCCG CGTACATGGT CGACGCGCCC GAGCAGATCG ATCCGGCGTG GGTCGCCGGC AAGCGCCGCA TCGGCGTGAC GGCCGGCGCA TCGGCGCCGG AAGTGCTCGC GCAGGCCGTG ATCGCCCGGC TGCGCGAACT CGGCGTGCGC AATGTCCGCG CACTCGAAGG CATCGAGGAA AACGTGGCGT TTCCGCTGCC GCGCGGGCTG AATCTGCCGC CCGCCGCC

Details for BuviA.17229.a.A1.GE32483

HARVESTED ON:	11/8/2011
SEQUENCED ON:	11/11/2011
EXPECTED MW:	37kDa
OBSERVED MW:	38kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	High Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	100
PERCENT COVERAGE:	93

Validated AA Sequence

Validated NT Sequence

tattaggcgg cgggcggcag attcagcccg cgcggcagcg gaaacgccac gttttcctcg atgccttcga gtgcgcggac attgcgcacg ccgagttcgc gcagccgggc gatcacggcc tgcgcgagca cttccggcgc cgatgcgccg gccgtcacgc cgatgcggcg cttgccggcg acccacgccg gatcgatctg ctcgggcgcg tcgaccatgt acgcggccac gccgcgcttc tccgcgactt cgcgcaggcg gctggaattc gaactgttcg ggctgccgac cacgatcacg acgtcgcact gcggcgccat gaacttcacc gcgtcctggc ggttctgcgt cgcatagcag atgtcctgct tcttcggctc gcggatcttc gggtacttcg ccttcagcgc gccgatgatt tcggctgcgt catcaacgga cagcgtcgtt tgcgtgacga gcgcgatgcg ctcgggatcg gcgagttcga gcttctggac gtcctcgacg ctttcgacca gatgcatgcc gcgctcgacc tgccccatcg tgccttcgac ttccgggtgg cccttgtggc cgatcatcac gatgtcgacg ccgtcctggc gcatcttcgc gacttcgacg tgcactttcg tgacgagcgg gcaggtcgcg tcgtaaatgc gcaggccgcg cacgtcggcc tcgtcgcgca cggctttcga cacgccatgc gcactgaaga tcaccgtgtt gccggccggc acttcctcga gctcttcgac gaagatcgcg cccttcttcc tcagatcctc gaccacgtac ttgttgtgga cgatttcgtg acgcacgtag atcggtgcac cgtgcatcgc gatcgcgcgc tcgacgatct cgatcgcgcg gtcgacaccg gcgcagaagc cgcgcggctg tgccagcagg atttcggcat cggctgcgac ggtctgcccg ggcagcgtat cggtgttgct catcgaacca ggaccctggg tctgagcttc cagggtacc

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMSNTDTLPG QTVAADAEIL LAQPRGFCAG VDRAIEIVER AIAMHGAPIY VRHEIVHNKY VVEDLRKKGA IFVEELEEVP AGNTVIFSAH GVSKAVRDEA DVRGLRIYDA TCPLVTKVHV EVAKMRQDGV DIVMIGHKGH PEVEGTMGQV ERGMHLVESV EDVQKLELAD PERIALVTQT TLSVDDAAEI IGALKAKYPK IREPKKQDIC YATQNRQDAV KFMAPQCDVV IVVGSPNSSN SSRLREVAEK RGVAAYMVDA PEQIDPAWVA GKRRIGVTAG ASAPEVLAQA VIARLRELGV RNVRALEGIE ENVAFPLPRG LNLPPAA

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gagcaacacc gatacgctgc ccgggcagac cgtcgcagcc gatgccgaaa tcctgctggc acagccgcgc ggcttctgcg ccggtgtcga ccgcgcgatc gagatcgtcg agcgcgcgat cgcgatgcac ggtgcaccga tctacgtgcg tcacgaaatc gtccacaaca agtacgtggt cgaggatctg aggaagaagg gcgcgatctt cgtcgaagag ctcgaggaag tgccggccgg caacacggtg atcttcagtg cgcatggcgt gtcgaaagcc gtgcgcgacg aggccgacgt gcgcggcctg cgcatttacg acgcgacctg cccgctcgtc acgaaagtgc acgtcgaagt cgcgaagatg cgccaggacg gcgtcgacat cgtgatgatc ggccacaagg gccacccgga agtcgaaggc acgatggggc aggtcgagcg cggcatgcat ctggtcgaaa gcgtcgagga cgtccagaag ctcgaactcg ccgatcccga gcgcatcgcg ctcgtcacgc aaacgacgct gtccgttgat gacgcagccg aaatcatcgg cgcgctgaag gcgaagtacc cgaagatccg cgagccgaag aagcaggaca tctgctatgc gacgcagaac cgccaggacg cggtgaagtt catggcgccg cagtgcgacg tcgtgatcgt ggtcggcagc ccgaacagtt cgaattccag ccgcctgcgc gaagtcgcgg agaagcgcgg cgtggccgcg tacatggtcg acgcgcccga gcagatcgat ccggcgtggg tcgccggcaa gcgccgcatc ggcgtgacgg ccggcgcatc ggcgccggaa gtgctcgcgc aggccgtgat cgcccggctg cgcgaactcg gcgtgcgcaa tgtccgcgca ctcgaaggca tcgaggaaaa cgtggcgttt ccgctgccgc gcgggctgaa tctgccgccc gccgcctaaa cagcacgaac aagttctgca gccaagcttc tcgaggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaa

Details for BuviA.17229.a.A1.PS01328

PURIFICATION DATe:	1/5/2012
CONCENTRATION:	43.88mg/ml
OBSERVED MW:	data unavailable
EXPRESSION LEVEL:	Moderate Expression
PROTEIN PURIFICATION BUFFER:	20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	7
VIAL VOLUME:	110µl
PERCENT IDENTITY:	100
PERCENT COVERAGE:	93

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GAGCAACACC GATACGCTGC CCGGGCAGAC CGTCGCAGCC GATGCCGAAA TCCTGCTGGC ACAGCCGCGC GGCTTCTGCG CCGGTGTCGA CCGCGCGATC GAGATCGTCG AGCGCGCGAT CGCGATGCAC GGTGCACCGA TCTACGTGCG TCACGAAATC GTCCACAACA AGTACGTGGT CGAGGATCTG AGGAAGAAGG GCGCGATCTT CGTCGAAGAG CTCGAGGAAG TGCCGGCCGG CAACACGGTG ATCTTCAGTG CGCATGGCGT GTCGAAAGCC GTGCGCGACG AGGCCGACGT GCGCGGCCTG CGCATTTACG ACGCGACCTG CCCGCTCGTC ACGAAAGTGC ACGTCGAAGT CGCGAAGATG CGCCAGGACG GCGTCGACAT CGTGATGATC GGCCACAAGG GCCACCCGGA AGTCGAAGGC ACGATGGGGC AGGTCGAGCG CGGCATGCAT CTGGTCGAAA GCGTCGAGGA CGTCCAGAAG CTCGAACTCG CCGATCCCGA GCGCATCGCG CTCGTCACGC AAACGACGCT GTCCGTTGAT GACGCAGCCG AAATCATCGG CGCGCTGAAG GCGAAGTACC CGAAGATCCG CGAGCCGAAG AAGCAGGACA TCTGCTATGC GACGCAGAAC CGCCAGGACG CGGTGAAGTT CATGGCGCCG CAGTGCGACG TCGTGATCGT GGTCGGCAGC CCGAACAGTT CGAATTCCAG CCGCCTGCGC GAAGTCGCGG AGAAGCGCGG CGTGGCCGCG TACATGGTCG ACGCGCCCGA GCAGATCGAT CCGGCGTGGG TCGCCGGCAA GCGCCGCATC GGCGTGACGG CCGGCGCATC GGCGCCGGAA GTGCTCGCGC AGGCCGTGAT CGCCCGGCTG CGCGAACTCG GCGTGCGCAA TGTCCGCGCA CTCGAAGGCA TCGAGGAAAA CGTGGCGTTT CCGCTGCCGC GCGGGCTGAA TCTGCCGCCC GCCGCCTAAA CAGCACGAAC AAGTTCTGCA GCCAAGCTTC TCGAGGATCC GGCTGCTAAC AAAGCCCGAA AGGAAGCTGA GTTGGCTGCT GCCACCGCTG AGCAATAACT AGCATAACCC CTTGGGGCCT CTAAACGGGT CTTGAGGGGT TTTTTGCTGA AAGGAGGAAC TATATCCGGA TATCCACAGG ACGGGTGTGG TCGCCATGAT CGCGTAGTCG ATAGTGGCTC CAAGTAGCGA AGCGAGCAGG ACTGGGCGGC GGCCAAAGCG GTCGGACAGT GCTCCGAGAA CGGGTGCGCA TAGAAATTGC ATCAACGCAT ATAGCGCTAG CAGCACGCCA TAGTGACTGG CGATGCTGTC GGAATGGACG ATATCCCGCA AGAGGCCCGG CAGTACCGGC ATAACCAAGC CTATGCCTAC AGCATCCAGG GTGACGGTGC CGAGGATGAC GATGAGCGCA TTGTTAGATT TCATACACGG TGCCTGACTG CGTTAGCAAT TTAACTGTGA TAAACTACCG CATTAAAGCT TATCGATGAT AAGCTGTCAA ACATGAGAA