SSGCID: Target Page

BuviA.16001.a

Beta-lactamase domain protein

CENTER ID:	BuviA.16001.a
ORGANISM:	Burkholderia vietnamiensis G4
ASSOCIATED DISEASE:
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	True
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BuviA.16001.a.B1.GE39334	Full length( BuviA.16001.a )		1	258

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|269482.11.peg.6406
RefSeq:	YP_001120009.1
UniProt:	A4JFX1

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MRFASLGSGS EGNALVVEVA SGTTTTRVLL DCGFSAKEVE RRLGRLNLGV DDLDAILITH EHSDHVGSAL TLARRASLPL YMSWGTARAV GADEADVDLH VLWGDETAAI RDLAVIPYTV PHDAREPLQF VFMDGSRRLG VLTDVGMATP HITTVLSGCD ALVLESNHDT AMLAGSRYPQ SLKARIGGSH GHLSNDAAAG ILASLERSRL RHLVAAHLSQ QNNRPELARQ AFAGVLGTDG DEVVVATQDA GFDWLALD

NT Sequence

GTGCGCTTCG CGAGCCTTGG TAGCGGCAGC GAAGGCAACG CGCTGGTCGT CGAAGTCGCG AGCGGCACGA CGACCACGCG CGTGCTGCTC GACTGCGGTT TTTCTGCGAA GGAGGTCGAG CGCCGCCTCG GCCGCCTGAA TCTCGGCGTC GACGATCTCG ATGCGATTCT GATCACCCAC GAGCACAGCG ACCACGTCGG CAGCGCGTTG ACGCTGGCGC GGCGCGCTTC GCTGCCGCTC TACATGAGCT GGGGCACGGC GCGCGCGGTG GGCGCGGACG AGGCCGATGT CGATCTGCAC GTGCTGTGGG GCGACGAGAC GGCCGCCATC CGCGATCTGG CCGTGATCCC TTATACGGTG CCGCACGACG CGCGCGAGCC GCTCCAGTTC GTCTTTATGG ACGGTAGCCG CCGGCTCGGC GTGCTGACCG ACGTCGGGAT GGCCACGCCG CACATCACGA CCGTGCTGAG CGGCTGCGAC GCGCTGGTGC TCGAATCGAA CCACGATACG GCGATGCTCG CCGGAAGCCG CTATCCGCAG TCGCTGAAGG CGCGGATCGG CGGCAGTCAC GGCCATCTGA GCAACGATGC GGCGGCCGGC ATTCTCGCCT CGCTCGAACG TAGCCGGCTG CGGCATCTGG TGGCCGCGCA TCTGAGCCAG CAGAACAACC GTCCCGAACT GGCCCGCCAG GCGTTTGCGG GCGTGCTGGG CACCGATGGC GACGAGGTGG TCGTGGCGAC GCAGGACGCG GGTTTCGACT GGCTGGCGCT CGAC

Details for BuviA.16001.a.B1.GE39334

HARVESTED ON:	7/10/2015
SEQUENCED ON:	7/6/2015
EXPECTED MW:	28kDa
OBSERVED MW:	28kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	Insoluble
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY:	data unavailable
PERCENT COVERAGE:	data unavailable

Validated AA Sequence

sequence unavailable

Validated NT Sequence

sequence unavailable

Expected Protein Sequence

MAHHHHHHMR FASLGSGSEG NALVVEVASG TTTTRVLLDC GFSAKEVERR LGRLNLGVDD LDAILITHEH SDHVGSALTL ARRASLPLYM SWGTARAVGA DEADVDLHVL WGDETAAIRD LAVIPYTVPH DAREPLQFVF MDGSRRLGVL TDVGMATPHI TTVLSGCDAL VLESNHDTAM LAGSRYPQSL KARIGGSHGH LSNDAAAGIL ASLERSRLRH LVAAHLSQQN NRPELARQAF AGVLGTDGDE VVVATQDAGF DWLALD

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgtgcgct tcgcgagcct tggtagcggc agcgaaggca acgcgctggt cgtcgaagtc gcgagcggca cgacgaccac gcgcgtgctg ctcgactgcg gtttttctgc gaaggaggtc gagcgccgcc tcggccgcct gaatctcggc gtcgacgatc tcgatgcgat tctgatcacc cacgagcaca gcgaccacgt cggcagcgcg ttgacgctgg cgcggcgcgc ttcgctgccg ctctacatga gctggggcac ggcgcgcgcg gtgggcgcgg acgaggccga tgtcgatctg cacgtgctgt ggggcgacga gacggccgcc atccgcgatc tggccgtgat cccttatacg gtgccgcacg acgcgcgcga gccgctccag ttcgtcttta tggacggtag ccgccggctc ggcgtgctga ccgacgtcgg gatggccacg ccgcacatca cgaccgtgct gagcggctgc gacgcgctgg tgctcgaatc gaaccacgat acggcgatgc tcgccggaag ccgctatccg cagtcgctga aggcgcggat cggcggcagt cacggccatc tgagcaacga tgcggcggcc ggcattctcg cctcgctcga acgtagccgg ctgcggcatc tggtggccgc gcatctgagc cagcagaaca accgtcccga actggcccgc caggcgtttg cgggcgtgct gggcaccgat ggcgacgagg tggtcgtggc gacgcaggac gcgggtttcg actggctggc gctcgactga gtaagatagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaattcttga agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgcagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatata tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag ctgcggtaaa gctcatcagc gtggtcgtga agcgattcac agatgtctgc ctgttcatcc gcgtccagct cgttgagttt ctccagaagc gttaatgtct ggcttctgat aaagcgggcc atgttaaggg cggttttttc ctgtttggtc actgatgcct ccgtgtaagg gggatttctg ttcatggggg taatgatacc gatgaaacga gagaggatgc tcacgatacg ggttactgat gatgaacatg cccggttact ggaacgttgt gagggtaaac aactggcggt atggatgcgg cgggaccaga gaaaaatcac tcagggtcaa tgccagcgct tcgttaatac agatgtaggt gttccacagg gtagccagca gcatcctgcg atgcagatcc ggaacataat ggtgcagggc gctgacttcc gcgtttccag actttacgaa acacggaaac cgaagaccat tcatgttgtt gctcaggtcg cagacgtttt gcagcagcag tcgcttcacg ttcgctcgcg tatcggtgat tcattctgct aaccagtaag gcaaccccgc cagcctagcc gggtcctcaa cgacaggagc acgatcatgc gcacccgtgg ccaggaccca acgctgcccg agatgcgccg cgtgcggctg ctggagatgg cggacgcgat ggatatgttc tgccaagggt tggtttgcgc attcacagtt ctccgcaaga attgattggc tccaattctt ggagtggtga atccgttagc gaggtgccgc cggcttccat tcaggtcgag gtggcccggc tccatgcacc gcgacgcaac gcggggaggc agacaaggta tagggcggcg cctacaatcc atgccaaccc gttccatgtg ctcgccgagg cggcataaat cgccgtgacg atcagcggtc cagtgatcga agttaggctg gtaagagccg cgagcgatcc ttgaagctgt ccctgatggt cgtcatctac ctgcctggac agcatggcct gcaacgcggg catcccgatg ccgccggaag cgagaagaat cataatgggg aaggccatcc agcctcgcgt cgcgaacgcc agcaagacgt agcccagcgc gtcggccgcc atgccggcga taatggcctg cttctcgccg aaacgtttgg tggcgggacc agtgacgaag gcttgagcga gggcgtgcaa gattccgaat accgcaagcg acaggccgat catcgtcgcg ctccagcgaa agcggtcctc gccgaaaatg acccagagcg ctgccggcac ctgtcctacg agttgcatga taaagaagac agtcataagt gcggcgacga tagtcatgcc ccgcgcccac cggaaggagc tgactgggtt gaaggctctc aagggcatcg gtcgacgctc tcccttatgc gactcctgca ttaggaagca gcccagtagt aggttgaggc cgttgagcac cgccgccgca aggaatggtg catgcaagga gatggcgccc aacagtcccc cggccacggg gcctgccacc atacccacgc cgaaacaagc gctcatgagc ccgaagtggc gagcccgatc ttccccatcg gtgatgtcgg cgatataggc gccagcaacc gcacctgtgg cgccggtgat gccggccacg atgcgtccgg cgtagaggat cgagatctcg atcccgcgaa at