SSGCID: Target Page

BuviA.16000.a

Squalene/phytoene synthase

CENTER ID:	BuviA.16000.a
ORGANISM:	Burkholderia vietnamiensis G4
ASSOCIATED DISEASE:
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	False
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BuviA.16000.a.B1.GE39333	Full length( BuviA.16000.a )		1	301

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|269482.11.peg.6121
RefSeq:	YP_001119699.1
UniProt:	A4JF11

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MPRSTCRPSA AARIPEPASA TIPPVNFDDY CQHKAAPAGS SVYYALRQAP RATEPRLTAL FALRRELEET VKEISDPTVG HTKLAWWHKE LAALAAGAPT HPVTKSLAQH HPTIAAEADA LRTLVNGFGM DLEQARYLDF ANLQRYIAQV GGAFASLVAR ASAANPADPQ SWAADAGHAL MLAQFVQELG NDARHGRIYL PIDELQRYNV TAADLLNRRY SPAFTELLQF QTSRARAALT AADAAIPAAE RRAQRTLRAQ IALAGALLDE IERDGYQVLH QRIALTPIRK LWIAWRAARR H

NT Sequence

ATGCCGCGCT CGACGTGTCG TCCAAGCGCC GCCGCGCGCA TCCCCGAACC TGCCTCCGCC ACGATCCCAC CCGTGAATTT CGACGACTAC TGCCAGCACA AGGCCGCCCC CGCGGGCTCC AGCGTCTACT ACGCGCTGCG CCAGGCGCCG CGCGCCACCG AGCCGCGTCT CACCGCCCTG TTCGCGCTGC GCCGCGAACT CGAGGAAACC GTCAAGGAAA TCAGCGACCC GACCGTAGGG CACACGAAGC TCGCGTGGTG GCACAAGGAA CTCGCAGCGC TCGCCGCCGG CGCGCCGACG CATCCGGTCA CGAAGTCGCT CGCGCAGCAT CATCCGACGA TCGCCGCCGA AGCCGATGCG CTGCGCACGC TCGTGAACGG TTTCGGAATG GACCTCGAAC AGGCGCGCTA TCTCGACTTC GCCAATCTGC AGCGCTACAT CGCGCAGGTC GGCGGCGCGT TTGCTTCGCT GGTCGCGCGC GCGAGCGCCG CGAATCCGGC CGACCCGCAA TCGTGGGCCG CGGACGCCGG CCACGCACTG ATGCTCGCGC AGTTCGTGCA GGAACTCGGC AACGATGCGC GCCATGGCCG CATCTATCTG CCGATCGACG AACTGCAACG CTACAACGTG ACCGCGGCCG ATCTGCTCAA TCGCCGCTAC AGCCCCGCCT TCACCGAGCT GCTGCAGTTT CAGACCAGCC GCGCACGCGC AGCGCTCACC GCCGCCGACG CGGCGATCCC CGCAGCGGAA CGCCGCGCGC AGCGCACGCT GCGCGCGCAG ATCGCGCTGG CCGGCGCACT GCTCGACGAA ATCGAACGCG ACGGCTACCA GGTGCTGCAT CAGCGCATCG CGCTGACGCC GATCCGCAAG CTGTGGATCG CGTGGCGCGC GGCGCGCCGC CAC

Details for BuviA.16000.a.B1.GE39333

HARVESTED ON:	7/10/2015
SEQUENCED ON:	7/6/2015
EXPECTED MW:	34kDa
OBSERVED MW:	34kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY:	99
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMP RSTCRPSAAA RIPEPASATI PPVNFDDYCQ HKAAPAGSSV YYALRQAPRA TEPRLTALFA LRRELEETVK EISDPTVGHT KLAWWHKELA ALAAGAPTHP VTKSLAQHHP TIAAEADALR TLVNGFGMDL EQARYLDFAN LQRYIAQVGG AFASLVARAS AANPADPQSW AADAGHALML AQFVQELGND ARHGRIYLPI DELQRYNVTA ADLLNRRYSP AFTELLQFQT SRARAALTAA DAAIPAAERR AQRTLRAQIA LAGALLDEIE RDGYQVLHQR IALTPIRKLW XXWRAARRH

Validated NT Sequence

atcttactca cttagtggcg gcgcgccgcg cgccannnna tccacagctt gcggatcggc gtcagcgcga tgcgctgatg cagcacctgg tagccgtcgc gttcgatttc gtcgagcagt gcgccggcca gcgcgatctg cgcgcgcagc gtgcgctgcg cgcggcgttc cgctgcgggg atcgccgcgt cggcggcggt gagcgctgcg cgtgcgcggc tggtctgaaa ctgcagcagc tcggtgaagg cggggctgta gcggcgattg agcagatcgg ccgcggtcac gttgtagcgt tgcagttcgt cgatcggcag atagatgcgg ccatggcgcg catcgttgcc gagttcctgc acgaactgcg cgagcatcag tgcgtggccg gcgtccgcgg cccacgattg cgggtcggcc ggattcgcgg cgctcgcgcg cgcgaccagc gaagcaaacg cgccgccgac ctgcgcgatg tagcgctgca gattggcgaa gtcgagatag cgcgcctgtt cgaggtccat tccgaaaccg ttcacgagcg tgcgcagcgc atcggcttcg gcggcgatcg tcggatgatg ctgcgcgagc gacttcgtga ccggatgcgt cggcgcgccg gcggcgagcg ctgcgagttc cttgtgccac cacgcgagct tcgtgtgccc tacggtcggg tcgctgattt ccttgacggt ttcctcgagt tcgcggcgca gcgcgaacag ggcggtgaga cgcggctcgg tggcgcgcgg cgcctggcgc agcgcgtagt agacgctgga gcccgcgggg gcggccttgt gctggcagta gtcgtcgaaa ttcacgggtg ggatcgtggc ggaggcaggt tcggggatgc gcgcggcggc gcttggacga cacgtcgagc gcggcatatg gtggtggtgg tggtgagcca t

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgccgc gctcgacgtg tcgtccaagc gccgccgcgc gcatccccga acctgcctcc gccacgatcc cacccgtgaa tttcgacgac tactgccagc acaaggccgc ccccgcgggc tccagcgtct actacgcgct gcgccaggcg ccgcgcgcca ccgagccgcg tctcaccgcc ctgttcgcgc tgcgccgcga actcgaggaa accgtcaagg aaatcagcga cccgaccgta gggcacacga agctcgcgtg gtggcacaag gaactcgcag cgctcgccgc cggcgcgccg acgcatccgg tcacgaagtc gctcgcgcag catcatccga cgatcgccgc cgaagccgat gcgctgcgca cgctcgtgaa cggtttcgga atggacctcg aacaggcgcg ctatctcgac ttcgccaatc tgcagcgcta catcgcgcag gtcggcggcg cgtttgcttc gctggtcgcg cgcgcgagcg ccgcgaatcc ggccgacccg caatcgtggg ccgcggacgc cggccacgca ctgatgctcg cgcagttcgt gcaggaactc ggcaacgatg cgcgccatgg ccgcatctat ctgccgatcg acgaactgca acgctacaac gtgaccgcgg ccgatctgct caatcgccgc tacagccccg ccttcaccga gctgctgcag tttcagacca gccgcgcacg cgcagcgctc accgccgccg acgcggcgat ccccgcagcg gaacgccgcg cgcagcgcac gctgcgcgcg cagatcgcgc tggccggcgc actgctcgac gaaatcgaac gcgacggcta ccaggtgctg catcagcgca tcgcgctgac gccgatccgc aagctgtgga tcgcgtggcg cgcggcgcgc cgccactgag taagatagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aattcttgaa gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgcagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat acactccgct atcgctacgt gactgggtca tggctgcgcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgaggcagc tgcggtaaag ctcatcagcg tggtcgtgaa gcgattcaca gatgtctgcc tgttcatccg cgtccagctc gttgagtttc tccagaagcg ttaatgtctg gcttctgata aagcgggcca tgttaagggc ggttttttcc tgtttggtca ctgatgcctc cgtgtaaggg ggatttctgt tcatgggggt aatgataccg atgaaacgag agaggatgct cacgatacgg gttactgatg atgaacatgc ccggttactg gaacgttgtg agggtaaaca actggcggta tggatgcggc gggaccagag aaaaatcact cagggtcaat gccagcgctt cgttaataca gatgtaggtg ttccacaggg tagccagcag catcctgcga tgcagatccg gaacataatg gtgcagggcg ctgacttccg cgtttccaga ctttacgaaa cacggaaacc gaagaccatt catgttgttg ctcaggtcgc agacgttttg cagcagcagt cgcttcacgt tcgctcgcgt atcggtgatt cattctgcta accagtaagg caaccccgcc agcctagccg ggtcctcaac gacaggagca cgatcatgcg cacccgtggc caggacccaa cgctgcccga gatgcgccgc gtgcggctgc tggagatggc ggacgcgatg gatatgttct gccaagggtt ggtttgcgca ttcacagttc tccgcaagaa ttgattggct ccaattcttg gagtggtgaa tccgttagcg aggtgccgcc ggcttccatt caggtcgagg tggcccggct ccatgcaccg cgacgcaacg cggggaggca gacaaggtat agggcggcgc ctacaatcca tgccaacccg ttccatgtgc tcgccgaggc ggcataaatc gccgtgacga tcagcggtcc agtgatcgaa gttaggctgg taagagccgc gagcgatcct tgaagctgtc cctgatggtc gtcatctacc tgcctggaca gcatggcctg caacgcgggc atcccgatgc cgccggaagc gagaagaatc ataatgggga aggccatcca gcctcgcgtc gcgaacgcca gcaagacgta gcccagcgcg tcggccgcca tgccggcgat aatggcctgc ttctcgccga aacgtttggt ggcgggacca gtgacgaagg cttgagcgag ggcgtgcaag attccgaata ccgcaagcga caggccgatc atcgtcgcgc tccagcgaaa gcggtcctcg ccgaaaatga cccagagcgc tgccggcacc tgtcctacga gttgcatgat aaagaagaca gtcataagtg cggcgacgat agtcatgccc cgcgcccacc ggaaggagct gactgggttg aaggctctca agggcatcgg tcgacgctct cccttatgcg actcctgcat taggaagcag cccagtagta ggttgaggcc gttgagcacc gccgccgcaa ggaatggtgc atgcaaggag atggcgccca acagtccccc ggccacgggg cctgccacca tacccacgcc gaaacaagcg ctcatgagcc cgaagtggcg agcccgatct tccccatcgg tgatgtcggc gatataggcg ccagcaaccg cacctgtggc gccggtgatg ccggccacga tgcgtccggc gtagaggatc gagatctcga tcccgcgaaa t