BuviA.12800.y

Transcriptional regulator, LysR family

CENTER ID: BuviA.12800.y
ORGANISM: Burkholderia vietnamiensis G4
ASSOCIATED DISEASE:
CURRENT STATUS: soluble
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BuviA.12800.y.A1.GE32478 Full length( BuviA.12800.y ) 1 313
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|269482.11.peg.1243
RefSeq: YP_001115035.1
UniProt: A4JR77

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MAGFDERMLN GMGVLAAIVD TGSFAGAGEI LDMSQSGVSR AIARLEGRLG VRLLERTTRS VTLTDEGRRF YEQVMPLLGA LEEATTHATQ GATAVRGRLR VNVDPFFSRF VLGPQLGSFI EEYPELRVEL VTRDQLGDMI ADGFELAVRF GHPQPSTLVA RKLLETRILT VAAPSYLKRH GRPLQPAGLE RDTHACIRYR DPATGHPFDW EFHRGRKKLV VDVAGRLTVN DVGTMHSVCL AGHAIAQVME LGVEAPLADG RLVDLFPDWP DDRFPLYALY PSRRYVPAKV RAFLDFIVSL AAPAAHAPAR KAR
NT Sequence
ATGGCCGGCT TTGACGAACG TATGCTGAAC GGCATGGGCG TGTTGGCCGC GATCGTCGAT ACCGGCAGCT TTGCTGGCGC GGGCGAAATA CTCGACATGT CGCAGTCGGG CGTGAGCCGG GCGATCGCGC GGCTGGAAGG CCGGCTCGGC GTGCGCCTGC TGGAGCGCAC GACGCGCTCC GTCACGCTCA CCGACGAAGG CCGCCGTTTC TACGAGCAGG TGATGCCGCT GCTGGGGGCG CTCGAAGAAG CGACGACCCA TGCGACGCAG GGTGCGACGG CGGTGCGCGG CCGCCTGCGC GTGAACGTCG ATCCGTTTTT CTCGCGGTTC GTGCTCGGTC CGCAGTTGGG TTCGTTCATC GAGGAATATC CGGAGCTCAG GGTCGAACTG GTGACGCGCG ATCAGCTTGG CGACATGATC GCGGACGGCT TCGAGCTGGC GGTGCGTTTC GGGCATCCGC AGCCGTCGAC GCTCGTCGCG CGCAAGCTGC TGGAGACGCG CATTCTCACG GTCGCCGCGC CGTCGTATCT GAAGCGGCAT GGGCGGCCAC TGCAACCCGC CGGTCTGGAG CGCGACACGC ATGCGTGCAT CCGCTATCGC GATCCGGCCA CCGGGCATCC GTTCGACTGG GAGTTTCATC GCGGGCGCAA GAAGCTCGTC GTCGATGTCG CGGGCCGGTT GACGGTGAAC GACGTCGGGA CGATGCACAG CGTCTGTCTC GCCGGCCACG CGATCGCGCA GGTGATGGAA CTGGGCGTCG AAGCGCCACT CGCGGACGGC CGGCTCGTCG ACCTGTTTCC CGACTGGCCC GATGACCGTT TCCCGCTGTA TGCGCTGTAT CCGTCGCGGC GCTACGTGCC GGCGAAGGTG CGTGCTTTTC TCGACTTCAT CGTCTCGCTT GCGGCGCCGG CCGCTCATGC GCCGGCCCGC AAGGCTCGT
Details for BuviA.12800.y.A1.GE32478
HARVESTED ON: 11/8/2011
SEQUENCED ON: 11/11/2011
EXPECTED MW: 36kDa
OBSERVED MW: 37kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 98
PERCENT COVERAGE: 80
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMAGFDERML NGMGVLAAIV DTGSFAGAGE ILDMSQSGVS RAIARLEGRL GVRLLERTTR SVTLTDEGRR FYEQVMPLLG ALEEATTHAT QGATAVRGRL RVNVDPFFSR FVLGPQLGSF IEEYPELRVE LVTRDQLGDM IADGFELAVR FGHPQPSTLV ARKLLETRIL TVAAPSYLKR HGRPLQPAGL ERDTHACIRY RDPATGHPFD WEFHRGRKKL VVDVAGRLTV NDVXTMHSVC LXXHAIAX
Validated NT Sequence
tttnttnnnn ttaagaagga gatataccat ggctcatcac catcaccatc atatgggtac cctggaagct cagacccagg gtcctggttc gatggccggc tttgacgaac gtatgctgaa cggcatgggc gtgttggccg cgatcgtcga taccggcagc tttgctggcg cgggcgaaat actcgacatg tcgcagtcgg gcgtgagccg ggcgatcgcg cggctggaag gccggctcgg cgtgcgcctg ctggagcgca cgacgcgctc cgtcacgctc accgacgaag gccgccgttt ctacgagcag gtgatgccgc tgctgggggc gctcgaagaa gcgacgaccc atgcgacgca gggtgcgacg gcggtgcgcg gccgcctgcg cgtgaacgtc gatccgtttt tctcgcggtt cgtgctcggt ccgcagttgg gttcgttcat cgaggaatat ccggagctca gggtcgaact ggtgacgcgc gatcagcttg gcgacatgat cgcggacggc ttcgagctgg cggtgcgttt cgggcatccg cagccgtcga cgctcgtcgc gcgcaagctg ctggagacgc gcattctcac ggtcgccgcg ccgtcgtatc tgaagcggca tgggcggcca ctgcaacccg ccggtctgga gcgcgacacg catgcgtgca tccgctatcg cgatccggcc accgggcatc cgttcgactg ggagtttcat cgcgggcgca agaagctcgt cgtcgatgtc gcgggccggt tgacggtgaa cgacgtcngg acgatgcaca gcgtctgtct cgncngncac gcgatcgcgc nntgatggaa ctgggcgtcg aagcgccact cgcggacggc cngctcgtca nctgtttccc gactggnccg atgaccgttt cccgctgtat gcgctgtatc cgtcgcggcg ctncntgccg gcgaanngcg tgcttttctc gacttcatcg tctcgcttgn gnngccggcc gctcatgngc cggccnncan ggntcgtnan naaacagcac gannnagttc tgcagcnnnc ttcncnanga tcnggctgct annaannncg aangnanctn annnnnnnnn nnnnnnnnnn nnngng
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMAGFDERML NGMGVLAAIV DTGSFAGAGE ILDMSQSGVS RAIARLEGRL GVRLLERTTR SVTLTDEGRR FYEQVMPLLG ALEEATTHAT QGATAVRGRL RVNVDPFFSR FVLGPQLGSF IEEYPELRVE LVTRDQLGDM IADGFELAVR FGHPQPSTLV ARKLLETRIL TVAAPSYLKR HGRPLQPAGL ERDTHACIRY RDPATGHPFD WEFHRGRKKL VVDVAGRLTV NDVGTMHSVC LAGHAIAQVM ELGVEAPLAD GRLVDLFPDW PDDRFPLYAL YPSRRYVPAK VRAFLDFIVS LAAPAAHAPA RKAR
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggccggcttt gacgaacgta tgctgaacgg catgggcgtg ttggccgcga tcgtcgatac cggcagcttt gctggcgcgg gcgaaatact cgacatgtcg cagtcgggcg tgagccgggc gatcgcgcgg ctggaaggcc ggctcggcgt gcgcctgctg gagcgcacga cgcgctccgt cacgctcacc gacgaaggcc gccgtttcta cgagcaggtg atgccgctgc tgggggcgct cgaagaagcg acgacccatg cgacgcaggg tgcgacggcg gtgcgcggcc gcctgcgcgt gaacgtcgat ccgtttttct cgcggttcgt gctcggtccg cagttgggtt cgttcatcga ggaatatccg gagctcaggg tcgaactggt gacgcgcgat cagcttggcg acatgatcgc ggacggcttc gagctggcgg tgcgtttcgg gcatccgcag ccgtcgacgc tcgtcgcgcg caagctgctg gagacgcgca ttctcacggt cgccgcgccg tcgtatctga agcggcatgg gcggccactg caacccgccg gtctggagcg cgacacgcat gcgtgcatcc gctatcgcga tccggccacc gggcatccgt tcgactggga gtttcatcgc gggcgcaaga agctcgtcgt cgatgtcgcg ggccggttga cggtgaacga cgtcgggacg atgcacagcg tctgtctcgc cggccacgcg atcgcgcagg tgatggaact gggcgtcgaa gcgccactcg cggacggccg gctcgtcgac ctgtttcccg actggcccga tgaccgtttc ccgctgtatg cgctgtatcc gtcgcggcgc tacgtgccgg cgaaggtgcg tgcttttctc gacttcatcg tctcgcttgc ggcgccggcc gctcatgcgc cggcccgcaa ggctcgttaa acagcacgaa caagttctgc agccaagctt ctcgaggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa