BuviA.12800.w

Transcriptional regulator, LysR family

CENTER ID: BuviA.12800.w
ORGANISM: Burkholderia vietnamiensis G4
ASSOCIATED DISEASE:
CURRENT STATUS: soluble
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BuviA.12800.w.A1.GE32476 Full length( BuviA.12800.w ) 1 311
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|269482.11.peg.3671
RefSeq: YP_001117364.1
UniProt: A4JNP6

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MDKVHTMSVF VKVVEMGSFT AVANHLDTTV GNISRAVSVL EDNLNTRLLQ RSTRRLVVTD AGRRFYERSV AILSDIERAE AEARDALLEP RGLLRVHAVP GLGRQLVSQA VLAYRSAYPE VSVDLMLSQR MPNLLEEQLD VAIVIARTLP DSAYVSQKIG TSHCILAASP DYLARHPAPE CVEDLVGHTC VLLSTVDYAP DEWHLQGRAG EAVYRPTGSH FSVNDMDAMS TVLREGAGIG LIAAFSAIDD LRKGSLVRVL PEFHTAQRNV YAVYSSRQFV DAKIKRFIDV LKVQVGDRLN AYADELAIMV A
NT Sequence
ATGGACAAGG TGCACACCAT GTCGGTGTTT GTGAAAGTGG TCGAGATGGG CAGTTTCACG GCTGTCGCAA ACCACCTCGA TACGACGGTC GGCAACATTT CTCGAGCGGT ATCTGTCCTC GAAGACAATC TCAACACGCG GTTGCTACAG CGCTCGACGC GACGGCTCGT CGTTACCGAC GCGGGGCGAC GTTTTTACGA ACGCAGTGTT GCGATCCTCT CGGACATCGA ACGGGCGGAA GCGGAAGCAC GCGATGCGTT GCTGGAGCCG CGTGGTCTAT TACGCGTGCA TGCGGTGCCG GGATTGGGGC GCCAACTCGT TTCGCAAGCC GTGCTTGCCT ATCGGAGCGC ATATCCGGAA GTATCGGTCG ATCTCATGCT GTCGCAGCGG ATGCCGAATC TGCTCGAAGA GCAACTCGAC GTGGCGATCG TGATCGCGCG CACGCTGCCC GACTCGGCGT ACGTGAGCCA GAAGATCGGA ACCAGTCATT GCATTCTCGC GGCGTCGCCC GACTATCTGG CACGGCATCC GGCTCCCGAG TGTGTGGAAG ACCTCGTCGG TCACACATGT GTGCTTCTGA GCACGGTCGA TTACGCGCCG GACGAATGGC ATCTGCAAGG CAGAGCAGGC GAGGCGGTGT ATCGTCCAAC GGGATCGCAC TTCAGTGTGA ATGACATGGA TGCGATGTCC ACGGTACTGC GGGAGGGCGC GGGAATTGGA TTGATCGCTG CCTTCTCGGC AATCGACGAT CTGCGCAAAG GATCGCTCGT TCGCGTCCTG CCGGAATTTC ACACCGCGCA GCGCAACGTA TATGCGGTGT ACTCGTCCCG TCAATTCGTC GACGCGAAAA TCAAACGGTT CATCGATGTC CTGAAGGTGC AGGTAGGGGA TCGGTTGAAT GCGTATGCGG ATGAGCTCGC GATCATGGTC GCA
Details for BuviA.12800.w.A1.GE32476
HARVESTED ON: 11/8/2011
SEQUENCED ON: 11/15/2011
EXPECTED MW: 36kDa
OBSERVED MW: 37kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 99
PERCENT COVERAGE: 65
Validated AA Sequence
MGSFTAVANH LDTTVGNISR AVSVLXDNLN TRLLQRSTRR LVVTDAGRRF YERSVAILSD IERAEAEARD ALLEPRGLLR VHAVPGLGRQ LVSQAVLAYR SAYPEVSVDL MLSQRMPNLL EEQLDVAIVI ARTLPDSAYV SQKIGTSHCI LAASPDYLAR HPAPECVEDL VGHTCVLLST VDYAPDEWHL QGRAGEAVYR PTGSHFSVND MDAMST
Validated NT Sequence
taccgtggac atcgcatcca tgtcattcac actgaagtgc gatcccgttg gacgatacac cgcctcgcct gctctgcctt gcagatgcca ttcgtccggc gcgtaatcga ccgtgctcag aagcacacat gtgtgaccga cgaggtcttc cacacactcg ggagccggat gccgtgccag atagtcgggc gacgccgcga gaatgcaatg actggttccg atcttctggc tcacgtacgc cgagtcgggc agcgtgcgcg cgatcacgat cgccacgtcg agttgctctt cgagcagatt cggcatccgc tgcgacagca tgagatcgac cgatacttcc ggatatgcgc tccgataggc aagcacggct tgcgaaacga gttggcgccc caatcccggc accgcatgca cgcgtaatag accacgcggc tccagcaacg catcgcgtgc ttccgcttcc gcccgttcga tgtccgagag gatcgcaaca ctgcgttcgt aaaaacgtcg ccccgcgtcg gtaacgacga gccgtcgcgt cgagcgctgt agcaaccgcg tgttgagatt gtcttngagg acagataccg ctcgagaaat gttgccgacc gtcgtatcga ggtggtttgc gacagccgtg aaactgccca tctcgaccac tttcacaaac acc
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMDKVHTMSV FVKVVEMGSF TAVANHLDTT VGNISRAVSV LEDNLNTRLL QRSTRRLVVT DAGRRFYERS VAILSDIERA EAEARDALLE PRGLLRVHAV PGLGRQLVSQ AVLAYRSAYP EVSVDLMLSQ RMPNLLEEQL DVAIVIARTL PDSAYVSQKI GTSHCILAAS PDYLARHPAP ECVEDLVGHT CVLLSTVDYA PDEWHLQGRA GEAVYRPTGS HFSVNDMDAM STVLREGAGI GLIAAFSAID DLRKGSLVRV LPEFHTAQRN VYAVYSSRQF VDAKIKRFID VLKVQVGDRL NAYADELAIM VA
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggacaaggtg cacaccatgt cggtgtttgt gaaagtggtc gagatgggca gtttcacggc tgtcgcaaac cacctcgata cgacggtcgg caacatttct cgagcggtat ctgtcctcga agacaatctc aacacgcggt tgctacagcg ctcgacgcga cggctcgtcg ttaccgacgc ggggcgacgt ttttacgaac gcagtgttgc gatcctctcg gacatcgaac gggcggaagc ggaagcacgc gatgcgttgc tggagccgcg tggtctatta cgcgtgcatg cggtgccggg attggggcgc caactcgttt cgcaagccgt gcttgcctat cggagcgcat atccggaagt atcggtcgat ctcatgctgt cgcagcggat gccgaatctg ctcgaagagc aactcgacgt ggcgatcgtg atcgcgcgca cgctgcccga ctcggcgtac gtgagccaga agatcggaac cagtcattgc attctcgcgg cgtcgcccga ctatctggca cggcatccgg ctcccgagtg tgtggaagac ctcgtcggtc acacatgtgt gcttctgagc acggtcgatt acgcgccgga cgaatggcat ctgcaaggca gagcaggcga ggcggtgtat cgtccaacgg gatcgcactt cagtgtgaat gacatggatg cgatgtccac ggtactgcgg gagggcgcgg gaattggatt gatcgctgcc ttctcggcaa tcgacgatct gcgcaaagga tcgctcgttc gcgtcctgcc ggaatttcac accgcgcagc gcaacgtata tgcggtgtac tcgtcccgtc aattcgtcga cgcgaaaatc aaacggttca tcgatgtcct gaaggtgcag gtaggggatc ggttgaatgc gtatgcggat gagctcgcga tcatggtcgc ataaacagca cgaacaagtt ctgcagccaa gcttctcgag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaa