BuviA.12800.v

Transcriptional regulator, LysR family

CENTER ID: BuviA.12800.v
ORGANISM: Burkholderia vietnamiensis G4
ASSOCIATED DISEASE:
CURRENT STATUS: soluble
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BuviA.12800.v.A1.GE32475 Full length( BuviA.12800.v ) 1 317
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|269482.11.peg.3599
RefSeq: YP_001117292.1
UniProt: A4JNH4

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MDTLQNMRVF TRVVETGSFT AAAQSLNSTT GAMSRAVSEL EARLRTRLMN RSTRRLALTS AGENYLRRCR QILADVDRAE EEASCAHERP AGVLRMHSFA SVGHHYVMPT LTRYHAQYPD VSIELSLSQR MPDLFDGTSD MAVVTASSLP DSELVSHLLG STFSILCASP EYVTRHGAPE HPTDLGAHAC LTLCTPAFPT HEWVLEGADG VEQIHVAGPV QTNTAESLAL AIRDGLGIGM LPLYSAIDAL QDGTLVRVLP SHILQKMNVY ALYPSRRFVD AKVRTWVEML RAQVPGMIAH DVEMLQAIAR ESEPQEA
NT Sequence
ATGGATACGC TACAAAACAT GCGCGTGTTT ACGCGCGTGG TCGAGACAGG CAGTTTCACT GCCGCCGCAC AATCGCTGAA CTCGACGACG GGCGCGATGT CGCGCGCGGT GTCCGAGCTC GAAGCGCGGC TGCGCACTCG TCTGATGAAT CGCTCGACCC GCCGCCTTGC GCTGACCTCG GCCGGCGAAA ATTATTTACG GCGCTGTCGC CAGATTCTCG CCGACGTCGA TCGCGCGGAG GAGGAGGCGA GCTGTGCGCA CGAGCGCCCG GCGGGCGTGC TGCGCATGCA CAGCTTCGCG AGCGTCGGCC ATCACTACGT GATGCCCACG CTCACGCGCT ATCACGCGCA GTATCCGGAC GTGTCGATCG AACTGTCGCT GTCGCAGCGG ATGCCCGACC TGTTCGACGG CACGAGCGAC ATGGCGGTCG TGACCGCGTC GTCGCTGCCC GATTCGGAAC TCGTGTCGCA CCTGCTCGGC TCGACCTTCA GCATCCTGTG CGCGTCGCCC GAATACGTGA CGCGCCACGG CGCGCCGGAG CATCCGACCG ATCTCGGCGC GCACGCGTGC CTGACGCTCT GCACGCCGGC GTTCCCGACG CACGAGTGGG TGCTCGAAGG CGCCGACGGC GTCGAGCAGA TCCATGTCGC CGGGCCGGTG CAAACCAACA CGGCCGAGTC GCTCGCGCTC GCGATCCGCG ACGGCCTCGG CATCGGCATG CTGCCGCTGT ATTCGGCGAT CGACGCGTTG CAGGACGGCA CGCTCGTGCG CGTGTTGCCG AGCCACATCC TGCAGAAGAT GAACGTGTAT GCGCTGTATC CGTCGCGCCG TTTCGTCGAC GCGAAGGTGC GTACCTGGGT CGAGATGCTG CGCGCGCAGG TGCCGGGGAT GATCGCGCAC GACGTCGAGA TGCTGCAGGC GATCGCACGC GAGAGCGAGC CGCAGGAAGC C
Details for BuviA.12800.v.A1.GE32475
HARVESTED ON: 11/8/2011
SEQUENCED ON: 11/11/2011
EXPECTED MW: 37kDa
OBSERVED MW: 38kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 97
PERCENT COVERAGE: 77
Validated AA Sequence
MGTLEAQTQG PGSMDTLQNM RVFTRVVETG SFTAAAQSLN STTGAMSRAV SELEARLXTR LMNRSTRRLA LTSAGENYLR RXRQILADVD RAEEEASCAH ERPAGVLRMH SFASVGHHYV MPTLTRYHAQ YPDVSIELSL SQRMPDLFDG TSDMAVVTAS SLPDSELVSH LLGSTFSILC ASPEYVTRHG APEHPTDLGA HACLTLCTPA FPTHEWVLEG ADGVEQIHVA GPVQTNTAES LALAIRDGLG IGMLPLXSAX XXL
Validated NT Sequence
gcaangngtn gntcgcngan nacagcggca gcatnccgat gccgaggccg tcgcggatcg cgagcgcgag cgactcggcc gtgttggttt gcaccggccc ggcgacatgg atctgctcga cgccgtcggc gccttcgagc acccactcgt gcgtcgggaa cgccggcgtg cagagcgtca ggcacgcgtg cgcgccgaga tcggtcggat gctccggcgc gccgtggcgc gtcacgtatt cgggcgacgc gcacaggatg ctgaaggtcg agccgagcag gtgcgacacg agttccgaat cgggcagcga cgacgcggtc acgaccgcca tgtcgctcgt gccgtcgaac aggtcgggca tccgctgcga cagcgacagt tcgatcgaca cgtccggata ctgcgcgtga tagcgcgtga gcgtgggcat cacgtagtga tggccgacgc tcgcgaagct gtgcatgcgc agcacgcccg ccgggcgctc gtgcgcacag ctcgcctcct cctccgcgcg atcgacgtcg gcgagaatct ggcgacngcg ccgtaaataa ttttcgccgg ccgaggtcag cgcaaggcgg cgggtcgagc gattcatcag acgagtgngc agccgcgctt cgagctcgga caccgcgcgc gacatcgcgc ccgtcgtcga gttcagcgat tgtgcggcgg cagtgaaact gcctgtctcg accacgcgcg taaacacgcg catgttttgt agcgtatcca tcgaaccagg accctgggtc tgagcttcca gggtacccat atgatgg
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMDTLQNMRV FTRVVETGSF TAAAQSLNST TGAMSRAVSE LEARLRTRLM NRSTRRLALT SAGENYLRRC RQILADVDRA EEEASCAHER PAGVLRMHSF ASVGHHYVMP TLTRYHAQYP DVSIELSLSQ RMPDLFDGTS DMAVVTASSL PDSELVSHLL GSTFSILCAS PEYVTRHGAP EHPTDLGAHA CLTLCTPAFP THEWVLEGAD GVEQIHVAGP VQTNTAESLA LAIRDGLGIG MLPLYSAIDA LQDGTLVRVL PSHILQKMNV YALYPSRRFV DAKVRTWVEM LRAQVPGMIA HDVEMLQAIA RESEPQEA
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggatacgcta caaaacatgc gcgtgtttac gcgcgtggtc gagacaggca gtttcactgc cgccgcacaa tcgctgaact cgacgacggg cgcgatgtcg cgcgcggtgt ccgagctcga agcgcggctg cgcactcgtc tgatgaatcg ctcgacccgc cgccttgcgc tgacctcggc cggcgaaaat tatttacggc gctgtcgcca gattctcgcc gacgtcgatc gcgcggagga ggaggcgagc tgtgcgcacg agcgcccggc gggcgtgctg cgcatgcaca gcttcgcgag cgtcggccat cactacgtga tgcccacgct cacgcgctat cacgcgcagt atccggacgt gtcgatcgaa ctgtcgctgt cgcagcggat gcccgacctg ttcgacggca cgagcgacat ggcggtcgtg accgcgtcgt cgctgcccga ttcggaactc gtgtcgcacc tgctcggctc gaccttcagc atcctgtgcg cgtcgcccga atacgtgacg cgccacggcg cgccggagca tccgaccgat ctcggcgcgc acgcgtgcct gacgctctgc acgccggcgt tcccgacgca cgagtgggtg ctcgaaggcg ccgacggcgt cgagcagatc catgtcgccg ggccggtgca aaccaacacg gccgagtcgc tcgcgctcgc gatccgcgac ggcctcggca tcggcatgct gccgctgtat tcggcgatcg acgcgttgca ggacggcacg ctcgtgcgcg tgttgccgag ccacatcctg cagaagatga acgtgtatgc gctgtatccg tcgcgccgtt tcgtcgacgc gaaggtgcgt acctgggtcg agatgctgcg cgcgcaggtg ccggggatga tcgcgcacga cgtcgagatg ctgcaggcga tcgcacgcga gagcgagccg caggaagcct aaacagcacg aacaagttct gcagccaagc ttctcgagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aa