BuviA.12800.t

Transcriptional regulator, LysR family

CENTER ID: BuviA.12800.t
ORGANISM: Burkholderia vietnamiensis G4
ASSOCIATED DISEASE:
CURRENT STATUS: soluble
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BuviA.12800.t.A1.GE32473 Full length( BuviA.12800.t ) 1 301
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|269482.11.peg.3134
RefSeq: YP_001116851.1
UniProt: A4JM83

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MVSLDRFAVF RAVVETGSFT AAAAQLNQAR AAVSFNIKQL EAELGVTLLT RTTRRVELTD AGERFYARCV RVLEEAESAI DDARSEHGGM HGVLRVTSTV EYGTMVVAPA LHAFMQRHPA LRVRLETHAT QVDLVRERVD VAIRLGRLEQ FQDLPYRGAC LATYEVRPVI APSLLAEAGL SQIGSPELLA RLPQLGHTRL ERIATWTLTD RDGNPHAFRP GAKPRVVVDN ASVLRVLARQ GSGVALLPEW LVRDDLASGA LVDALPGYRF PPQAVYALYP STAHVPQKVR AWVDFMKAYL A
NT Sequence
ATGGTGAGTC TCGATCGTTT CGCCGTCTTC CGCGCCGTCG TGGAAACGGG GTCGTTTACG GCCGCCGCCG CCCAGCTGAA CCAGGCGCGC GCCGCCGTCA GCTTCAACAT CAAGCAGCTG GAGGCCGAGC TCGGCGTCAC GCTGCTCACG CGCACCACGC GGCGCGTCGA GCTGACCGAC GCGGGCGAGC GCTTCTACGC GCGCTGCGTG CGCGTGCTGG AGGAAGCCGA AAGCGCGATC GACGACGCGC GCAGCGAGCA TGGCGGGATG CACGGCGTGC TGCGCGTGAC GTCGACGGTC GAGTACGGAA CGATGGTCGT CGCGCCGGCG CTGCACGCGT TCATGCAGCG CCACCCGGCG CTACGCGTGC GGCTCGAGAC CCATGCGACT CAGGTCGATC TGGTGCGCGA GCGTGTCGAC GTCGCGATTC GGCTCGGCCG CCTCGAGCAG TTCCAGGATC TGCCCTACCG CGGCGCGTGC CTCGCGACCT ACGAGGTGCG GCCGGTGATC GCGCCTTCGC TGCTCGCCGA GGCCGGCCTG TCGCAGATCG GCTCGCCGGA GCTGCTTGCC CGGCTGCCGC AACTCGGCCA TACGCGGCTC GAGCGGATCG CCACGTGGAC GCTCACCGAT CGCGACGGCA ACCCGCATGC GTTTCGCCCG GGCGCGAAGC CGCGCGTGGT GGTCGACAAC GCGTCGGTGC TGCGCGTGCT CGCGCGGCAA GGCAGCGGCG TCGCGCTGCT GCCCGAGTGG CTCGTGCGCG ACGACCTCGC GAGCGGCGCG CTGGTCGATG CGCTGCCCGG CTACCGGTTT CCGCCGCAGG CCGTCTATGC GCTGTACCCG TCGACCGCAC ACGTGCCGCA GAAGGTGCGC GCGTGGGTCG ACTTCATGAA GGCGTATCTG GCT
Details for BuviA.12800.t.A1.GE32473
HARVESTED ON: 11/8/2011
SEQUENCED ON: 11/11/2011
EXPECTED MW: 35kDa
OBSERVED MW: 35kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 100
PERCENT COVERAGE: 97
Validated AA Sequence
MGTLEAQTQG PGSMVSLDRF AVFRAVVETG SFTAAAAQLN QARAAVSFNI KQLEAELGVT LLTRTTRRVE LTDAGERFYA RCVRVLEEAE SAIDDARSEH GGMHGVLRVT STVEYGTMVV APALHAFMQR HPALRVRLET HATQVDLVRE RVDVAIRLGR LEQFQDLPYR GACLATYEVR PVIAPSLLAE AGLSQIGSPE LLARLPQLGH TRLERIATWT LTDRDGNPHA FRPGAKPRVV VDNASVLRVL ARQGSGVALL PEWLVRDDLA SGALVDALPG YRFPPQAVYA LYPSTAHVPQ KVRAWVDFMK AYLA
Validated NT Sequence
ttattaagcc agatacgcct tcatgaagtc gacccacgcg cgcaccttct gcggcacgtg tgcggtcgac gggtacagcg catagacggc ctgcggcgga aaccggtagc cgggcagcgc atcgaccagc gcgccgctcg cgaggtcgtc gcgcacgagc cactcgggca gcagcgcgac gccgctgcct tgccgcgcga gcacgcgcag caccgacgcg ttgtcgacca ccacgcgcgg cttcgcgccc gggcgaaacg catgcgggtt gccgtcgcga tcggtgagcg tccacgtggc gatccgctcg agccgcgtat ggccgagttg cggcagccgg gcaagcagct ccggcgagcc gatctgcgac aggccggcct cggcgagcag cgaaggcgcg atcaccggcc gcacctcgta ggtcgcgagg cacgcgccgc ggtagggcag atcctggaac tgctcgaggc ggccgagccg aatcgcgacg tcgacacgct cgcgcaccag atcgacctga gtcgcatggg tctcgagccg cacgcgtagc gccgggtggc gctgcatgaa cgcgtgcagc gccggcgcga cgaccatcgt tccgtactcg accgtcgacg tcacgcgcag cacgccgtgc atcccgccat gctcgctgcg cgcgtcgtcg atcgcgcttt cggcttcctc cagcacgcgc acgcagcgcg cgtagaagcg ctcgcccgcg tcggtcagct cgacgcgccg cgtggtgcgc gtgagcagcg tgacgccgag ctcggcctcc agctgcttga tgttgaagct gacggcggcg cgcgcctggt tcagctgggc ggcggcggcc gtaaacgacc ccgtttccac gacggcgcgg aagacggcga aacgatcgag actcaccatc gaaccaggac cctgggtctg agcttccagg gtacccatat gatg
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVSLDRFAV FRAVVETGSF TAAAAQLNQA RAAVSFNIKQ LEAELGVTLL TRTTRRVELT DAGERFYARC VRVLEEAESA IDDARSEHGG MHGVLRVTST VEYGTMVVAP ALHAFMQRHP ALRVRLETHA TQVDLVRERV DVAIRLGRLE QFQDLPYRGA CLATYEVRPV IAPSLLAEAG LSQIGSPELL ARLPQLGHTR LERIATWTLT DRDGNPHAFR PGAKPRVVVD NASVLRVLAR QGSGVALLPE WLVRDDLASG ALVDALPGYR FPPQAVYALY PSTAHVPQKV RAWVDFMKAY LA
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggtgagtctc gatcgtttcg ccgtcttccg cgccgtcgtg gaaacggggt cgtttacggc cgccgccgcc cagctgaacc aggcgcgcgc cgccgtcagc ttcaacatca agcagctgga ggccgagctc ggcgtcacgc tgctcacgcg caccacgcgg cgcgtcgagc tgaccgacgc gggcgagcgc ttctacgcgc gctgcgtgcg cgtgctggag gaagccgaaa gcgcgatcga cgacgcgcgc agcgagcatg gcgggatgca cggcgtgctg cgcgtgacgt cgacggtcga gtacggaacg atggtcgtcg cgccggcgct gcacgcgttc atgcagcgcc acccggcgct acgcgtgcgg ctcgagaccc atgcgactca ggtcgatctg gtgcgcgagc gtgtcgacgt cgcgattcgg ctcggccgcc tcgagcagtt ccaggatctg ccctaccgcg gcgcgtgcct cgcgacctac gaggtgcggc cggtgatcgc gccttcgctg ctcgccgagg ccggcctgtc gcagatcggc tcgccggagc tgcttgcccg gctgccgcaa ctcggccata cgcggctcga gcggatcgcc acgtggacgc tcaccgatcg cgacggcaac ccgcatgcgt ttcgcccggg cgcgaagccg cgcgtggtgg tcgacaacgc gtcggtgctg cgcgtgctcg cgcggcaagg cagcggcgtc gcgctgctgc ccgagtggct cgtgcgcgac gacctcgcga gcggcgcgct ggtcgatgcg ctgcccggct accggtttcc gccgcaggcc gtctatgcgc tgtacccgtc gaccgcacac gtgccgcaga aggtgcgcgc gtgggtcgac ttcatgaagg cgtatctggc ttaaacagca cgaacaagtt ctgcagccaa gcttctcgag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaa