BuviA.12800.s

Transcriptional regulator, LysR family

CENTER ID: BuviA.12800.s
ORGANISM: Burkholderia vietnamiensis G4
ASSOCIATED DISEASE:
CURRENT STATUS: soluble
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BuviA.12800.s.A2.GE32472 Mature protein( BuviA.12800.s ) 24 297
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|269482.11.peg.3027
RefSeq: YP_001116725.1
UniProt: A4JLV7

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MARRNLNDLL AFVTIAREGS FTRAAATLGV TQSALSQTIR AMEESLQIRL LSRTTRSVSP TPAGERLLQA IGHRLDEIDA ELDALTELRE KPAGTVRITC GDYMLHSILL PKLAPMLQEY PEIKLEFDVN YGFRDIVADR FDAGVRLGST IDKDMIAVPI GPALRMAVVG SPAYFANRPI PKKPQDLMHH NCINQRMPTS GGLYVWDFER RGQKLNVRVD GQLIFNTTQP QVDAALGGLG LTLLPEDELM PHIVSGRLVR VLEDWCPRFD GYHLYYPSRR QPSPAFSMVL DALRIRG
NT Sequence
GTGGCTCGAC GCAATCTGAA CGACCTTCTG GCTTTCGTAA CAATAGCCCG CGAAGGCAGC TTCACGCGTG CTGCCGCCAC GTTGGGCGTG ACGCAGTCGG CGCTGAGTCA AACGATTCGT GCAATGGAGG AAAGCCTGCA GATTCGCCTG CTGAGCCGCA CCACGCGCAG CGTCTCGCCC ACGCCCGCGG GGGAGCGGCT ACTGCAGGCG ATCGGTCATC GCCTGGACGA AATCGATGCC GAGCTGGATG CGCTCACAGA ATTGCGCGAA AAACCGGCCG GAACAGTCCG CATCACCTGC GGCGACTACA TGCTGCATAG CATTCTGCTG CCCAAGCTGG CGCCGATGCT TCAGGAATAT CCAGAAATCA AGCTGGAGTT TGATGTCAAC TACGGGTTTC GGGACATCGT GGCCGACCGC TTTGATGCGG GAGTGCGCCT AGGTAGTACG ATTGATAAAG ACATGATCGC GGTGCCCATT GGCCCTGCAT TGCGGATGGC AGTCGTCGGA TCTCCAGCCT ATTTTGCGAA TCGACCTATC CCGAAGAAAC CGCAGGACCT GATGCACCAC AACTGCATTA ATCAGCGCAT GCCGACCTCT GGCGGCCTCT ATGTCTGGGA TTTCGAGCGT CGCGGGCAAA AATTAAACGT GCGCGTCGAC GGGCAGTTGA TTTTCAATAC GACGCAGCCG CAGGTCGATG CGGCGCTTGG CGGCCTGGGA CTCACTCTAC TGCCCGAGGA CGAGCTGATG CCTCATATCG TCTCCGGCCG GCTCGTTCGC GTGCTGGAGG ACTGGTGTCC GCGGTTTGAC GGGTATCACC TGTACTATCC AAGCAGGCGC CAGCCCTCGC CGGCGTTCTC GATGGTGCTC GATGCGTTGC GAATTCGTGG C
Details for BuviA.12800.s.A2.GE32472
HARVESTED ON: 11/8/2011
SEQUENCED ON: 11/11/2011
EXPECTED MW: 32kDa
OBSERVED MW: 32kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 99
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMAAATLGVT QSALSQTIRA MEESLQIRLL SRTTRSVSPT PAGERLLQAI GHRLDEIDAE LDALTELREK PAGTVRITCG DYMLHSILLP KLAPMLQEYP EIKLEFDVNY GFRDIVADRF DAGVRLGSTI DKDMIAVPIG PALRMAVVGS PAYFANRPIP KKPQDLMHHN CINQRMPTSG GLYVWDFERR GQKLNVRVDG QLIFNTTQPQ VDAALGGLGL TLLPEDELMP HIVSGRLVRV LEDWCPRFDG YHLYYPSRRQ PSPAFSMVLD ALRIR
Validated NT Sequence
gccacgaatt cgcaacgcat cgagcaccat cgagaacgcc ggcgagggct ggcgcctgct tggatagtac aggtgatacc cgtcaaaccg cggacaccag tcctccagca cgcgaacgag ccggccggag acgatatgag gcatcagctc gtcctcgggc agtagagtga gtcccaggcc gccaagcgcc gcatcgacct gcggctgcgt cgtattgaaa atcaactgcc cgtcgacgcg cacgtttaat ttttgcccgc gacgctcgaa atcccagaca tagaggccgc cagaggtcgg catgcgctga ttaatgcagt tgtggtgcat caggtcctgc ggtttcttcg ggataggtcg attcgcaaaa taggctggag atccgacgac tgccatccgc aatgcagggc caatgggcac cgcgatcatg tctttatcaa tcgtactacc taggcgcact cccgcatcaa agcggtcggc cacgatgtcc cgaaacccgt agttgacatc aaactccagc ttgatttctg gatattcctg aagcatcggc gccagcttgg gcagcagaat gctatgcagc atgtagtcgc cgcaggtgat gcggactgtt ccggccggtt tttcgcgcaa ttctgtgagc gcatccagct cggcatcgat ttcgtccagg cgatgaccga tcgcctgcag tagccgctcc cccgcgggcg tgggcgagac gctgcgcgtg gtgcggctca gcaggcgaat ctgcaggctt tcctccattg cacgaatcgt ttgactcagc gccgactgcg tcacgcccaa cgtggcggca gccatcgaac caggaccctg ggtctgagct tccagggtac ccatatgatg gtgatggtga tgagccat
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMAAATLGVT QSALSQTIRA MEESLQIRLL SRTTRSVSPT PAGERLLQAI GHRLDEIDAE LDALTELREK PAGTVRITCG DYMLHSILLP KLAPMLQEYP EIKLEFDVNY GFRDIVADRF DAGVRLGSTI DKDMIAVPIG PALRMAVVGS PAYFANRPIP KKPQDLMHHN CINQRMPTSG GLYVWDFERR GQKLNVRVDG QLIFNTTQPQ VDAALGGLGL TLLPEDELMP HIVSGRLVRV LEDWCPRFDG YHLYYPSRRQ PSPAFSMVLD ALRIRG
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggctgccgcc acgttgggcg tgacgcagtc ggcgctgagt caaacgattc gtgcaatgga ggaaagcctg cagattcgcc tgctgagccg caccacgcgc agcgtctcgc ccacgcccgc gggggagcgg ctactgcagg cgatcggtca tcgcctggac gaaatcgatg ccgagctgga tgcgctcaca gaattgcgcg aaaaaccggc cggaacagtc cgcatcacct gcggcgacta catgctgcat agcattctgc tgcccaagct ggcgccgatg cttcaggaat atccagaaat caagctggag tttgatgtca actacgggtt tcgggacatc gtggccgacc gctttgatgc gggagtgcgc ctaggtagta cgattgataa agacatgatc gcggtgccca ttggccctgc attgcggatg gcagtcgtcg gatctccagc ctattttgcg aatcgaccta tcccgaagaa accgcaggac ctgatgcacc acaactgcat taatcagcgc atgccgacct ctggcggcct ctatgtctgg gatttcgagc gtcgcgggca aaaattaaac gtgcgcgtcg acgggcagtt gattttcaat acgacgcagc cgcaggtcga tgcggcgctt ggcggcctgg gactcactct actgcccgag gacgagctga tgcctcatat cgtctccggc cggctcgttc gcgtgctgga ggactggtgt ccgcggtttg acgggtatca cctgtactat ccaagcaggc gccagccctc gccggcgttc tcgatggtgc tcgatgcgtt gcgaattcgt ggctaaacag cacgaacaag ttctgcagcc aagcttctcg aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaa