BuviA.12800.r

Transcriptional regulator, LysR family

CENTER ID: BuviA.12800.r
ORGANISM: Burkholderia vietnamiensis G4
ASSOCIATED DISEASE:
CURRENT STATUS: soluble
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BuviA.12800.r.A1.GE32471 Full length( BuviA.12800.r ) 1 299
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|269482.11.peg.2889
RefSeq: YP_001116595.1
UniProt: A4JLH7

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MDYIDKLRVF RTVVETRSFT RAADIHGLAR PVVSRAVADL EARFGSRLLH RTTRQVSLTE TAERIYDRCA AVLDELDSLE AQALAHTREP EGLLRLVAHT TAALNRLVPL IAGFKAAHPK IRLDVTLTER PVDLVGEGYD IGIVVPYMLT SETTIVRLVE RIPVVIVATP AYLRTHPRPE TPAALAGHAF VPMSPSLRRP ALSFRDGNDM VTVPLSFDIS SNSPVFNREM VMRDFGIGVV PRTLVEPELA SGALVQLLEG ADLVDAFVEI KLAYANRALL PAKVKAFIDY AAAYGDGAR
NT Sequence
ATGGACTACA TCGACAAGTT GCGGGTCTTC CGGACGGTGG TCGAAACGCG CAGTTTCACG CGAGCGGCCG ATATCCATGG CCTGGCGCGG CCGGTGGTGT CGCGGGCCGT CGCCGATCTC GAGGCGCGCT TCGGTAGTCG CCTGCTGCAC CGGACCACGC GTCAGGTATC GCTCACCGAA ACCGCCGAGC GCATCTACGA CCGCTGTGCG GCGGTTCTCG ACGAACTCGA CTCGCTCGAA GCACAGGCGC TGGCGCACAC GCGCGAGCCG GAAGGGCTGC TGCGGCTCGT CGCGCACACG ACGGCCGCGC TGAACCGGCT CGTGCCGCTG ATCGCCGGTT TCAAGGCCGC GCATCCGAAA ATCCGCCTCG ATGTCACGCT GACCGAGCGC CCGGTCGATC TCGTCGGCGA AGGTTACGAC ATCGGCATCG TCGTCCCGTA CATGCTGACG AGCGAAACGA CGATCGTGCG GCTCGTGGAA CGCATCCCGG TGGTGATCGT CGCGACGCCC GCGTATCTGC GCACGCATCC GCGGCCCGAG ACTCCCGCCG CACTCGCCGG CCACGCGTTC GTGCCGATGT CGCCGTCGTT GCGCCGGCCC GCGCTGTCGT TCCGCGACGG CAACGACATG GTGACCGTGC CGCTGAGCTT CGACATTTCG TCGAACAGCC CCGTCTTCAA CCGCGAAATG GTGATGCGCG ACTTCGGCAT CGGCGTGGTG CCGAGGACGC TGGTCGAGCC CGAACTCGCG TCCGGTGCGC TCGTGCAATT GCTCGAAGGC GCCGATCTCG TCGACGCGTT CGTCGAGATC AAGCTCGCCT ATGCGAACCG CGCGCTGCTG CCCGCGAAGG TCAAGGCGTT CATCGACTAC GCAGCCGCCT ACGGCGACGG CGCACGC
Details for BuviA.12800.r.A1.GE32471
HARVESTED ON: 11/8/2011
SEQUENCED ON: 11/11/2011
EXPECTED MW: 35kDa
OBSERVED MW: 35kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 100
PERCENT COVERAGE: 97
Validated AA Sequence
MGTLEAQTQG PGSMDYIDKL RVFRTVVETR SFTRAADIHG LARPVVSRAV ADLEARFGSR LLHRTTRQVS LTETAERIYD RCAAVLDELD SLEAQALAHT REPEGLLRLV AHTTAALNRL VPLIAGFKAA HPKIRLDVTL TERPVDLVGE GYDIGIVVPY MLTSETTIVR LVERIPVVIV ATPAYLRTHP RPETPAALAG HAFVPMSPSL RRPALSFRDG NDMVTVPLSF DISSNSPVFN REMVMRDFGI GVVPRTLVEP ELASGALVQL LEGADLVDAF VEIKLAYANR ALLPAKVKAF IDYAAAYGDG AR
Validated NT Sequence
ttattagcgt gcgccgtcgc cgtaggcggc tgcgtagtcg atgaacgcct tgaccttcgc gggcagcagc gcgcggttcg cataggcgag cttgatctcg acgaacgcgt cgacgagatc ggcgccttcg agcaattgca cgagcgcacc ggacgcgagt tcgggctcga ccagcgtcct cggcaccacg ccgatgccga agtcgcgcat caccatttcg cggttgaaga cggggctgtt cgacgaaatg tcgaagctca gcggcacggt caccatgtcg ttgccgtcgc ggaacgacag cgcgggccgg cgcaacgacg gcgacatcgg cacgaacgcg tggccggcga gtgcggcggg agtctcgggc cgcggatgcg tgcgcagata cgcgggcgtc gcgacgatca ccaccgggat gcgttccacg agccgcacga tcgtcgtttc gctcgtcagc atgtacggga cgacgatgcc gatgtcgtaa ccttcgccga cgagatcgac cgggcgctcg gtcagcgtga catcgaggcg gattttcgga tgcgcggcct tgaaaccggc gatcagcggc acgagccggt tcagcgcggc cgtcgtgtgc gcgacgagcc gcagcagccc ttccggctcg cgcgtgtgcg ccagcgcctg tgcttcgagc gagtcgagtt cgtcgagaac cgccgcacag cggtcgtaga tgcgctcggc ggtttcggtg agcgatacct gacgcgtggt ccggtgcagc aggcgactac cgaagcgcgc ctcgagatcg gcgacggccc gcgacaccac cggccgcgcc aggccatgga tatcggccgc tcgcgtgaaa ctgcgcgttt cgaccaccgt ccggaagacc cgcaacttgt cgatgtagtc catcgaacca ggaccctggg tctgagcttc cagggtaccc atatgatggt gatggtgatg agc
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMDYIDKLRV FRTVVETRSF TRAADIHGLA RPVVSRAVAD LEARFGSRLL HRTTRQVSLT ETAERIYDRC AAVLDELDSL EAQALAHTRE PEGLLRLVAH TTAALNRLVP LIAGFKAAHP KIRLDVTLTE RPVDLVGEGY DIGIVVPYML TSETTIVRLV ERIPVVIVAT PAYLRTHPRP ETPAALAGHA FVPMSPSLRR PALSFRDGND MVTVPLSFDI SSNSPVFNRE MVMRDFGIGV VPRTLVEPEL ASGALVQLLE GADLVDAFVE IKLAYANRAL LPAKVKAFID YAAAYGDGAR
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggactacatc gacaagttgc gggtcttccg gacggtggtc gaaacgcgca gtttcacgcg agcggccgat atccatggcc tggcgcggcc ggtggtgtcg cgggccgtcg ccgatctcga ggcgcgcttc ggtagtcgcc tgctgcaccg gaccacgcgt caggtatcgc tcaccgaaac cgccgagcgc atctacgacc gctgtgcggc ggttctcgac gaactcgact cgctcgaagc acaggcgctg gcgcacacgc gcgagccgga agggctgctg cggctcgtcg cgcacacgac ggccgcgctg aaccggctcg tgccgctgat cgccggtttc aaggccgcgc atccgaaaat ccgcctcgat gtcacgctga ccgagcgccc ggtcgatctc gtcggcgaag gttacgacat cggcatcgtc gtcccgtaca tgctgacgag cgaaacgacg atcgtgcggc tcgtggaacg catcccggtg gtgatcgtcg cgacgcccgc gtatctgcgc acgcatccgc ggcccgagac tcccgccgca ctcgccggcc acgcgttcgt gccgatgtcg ccgtcgttgc gccggcccgc gctgtcgttc cgcgacggca acgacatggt gaccgtgccg ctgagcttcg acatttcgtc gaacagcccc gtcttcaacc gcgaaatggt gatgcgcgac ttcggcatcg gcgtggtgcc gaggacgctg gtcgagcccg aactcgcgtc cggtgcgctc gtgcaattgc tcgaaggcgc cgatctcgtc gacgcgttcg tcgagatcaa gctcgcctat gcgaaccgcg cgctgctgcc cgcgaaggtc aaggcgttca tcgactacgc agccgcctac ggcgacggcg cacgctaaac agcacgaaca agttctgcag ccaagcttct cgaggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaa