SSGCID: Target Page

BuviA.12800.q

Transcriptional regulator, LysR family

CENTER ID:	BuviA.12800.q
ORGANISM:	Burkholderia vietnamiensis G4
ASSOCIATED DISEASE:
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	True
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BuviA.12800.q.A1.GE32470	Full length( BuviA.12800.q )		1	323

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|269482.11.peg.2833
RefSeq:	YP_001116542.1
UniProt:	A4JLC4

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MADLRDVNLN RLAIFVAVVD AGSLTAAAER LGLAKTVVST HMQRLESEVG ANLLVRTTRR LSVTDAGRAF YDACRDILRA AEAALDAASS DAGPLRGTLR VSVPIDYGAL VVAPAIVALR DAHPALDVEL IANDRVVDLV ADNLDVAIRI GRLADSNYRA VQLGEYEKWL VASPAFVARY GLPRDPDALA ALPFVMLSTL PRPHTLELDH ARGAHASVRC VAPVVSNTAT ACRAIALAGG GFGLLTDFSI ADDVAAGRLV RLLPAWRSAR AGIHAVYPST RMPSRKVRAF VDAMKACIGT ARPTAPARAT ASKRAARRAP GAA

NT Sequence

ATGGCCGATC TACGCGACGT GAACCTGAAC CGGCTGGCGA TCTTCGTCGC GGTGGTCGAT GCCGGCTCGC TGACGGCCGC CGCCGAGCGG CTCGGGCTGG CGAAGACGGT CGTCAGCACG CACATGCAGC GGCTCGAATC CGAGGTCGGC GCGAACCTGC TGGTGCGCAC CACGCGGCGC CTGAGCGTGA CCGACGCGGG GCGTGCGTTC TACGATGCGT GCCGCGACAT CCTGCGTGCG GCGGAAGCCG CGCTCGACGC CGCGTCGTCC GACGCGGGGC CGCTGCGCGG CACGCTGCGC GTGAGCGTGC CGATCGACTA CGGCGCGCTG GTGGTCGCGC CGGCGATCGT CGCGTTGCGC GACGCGCATC CGGCGCTCGA CGTCGAGCTG ATCGCGAACG ACCGGGTCGT CGATCTCGTC GCGGACAACC TCGACGTCGC GATCCGCATC GGCCGGCTCG CCGATTCGAA CTACCGCGCA GTGCAACTCG GCGAATACGA GAAATGGCTG GTGGCGAGCC CCGCGTTCGT CGCGCGCTAC GGGCTGCCGC GCGATCCGGA CGCGCTCGCC GCGCTGCCGT TCGTGATGCT GTCGACGCTG CCGCGCCCGC ATACGCTCGA ACTCGACCAT GCGCGCGGCG CTCACGCGTC GGTGCGCTGC GTCGCGCCGG TCGTCTCGAA CACCGCGACC GCGTGCCGCG CGATCGCGCT CGCGGGCGGC GGCTTCGGGC TCCTGACGGA TTTCTCGATC GCCGACGACG TGGCGGCCGG CCGGCTGGTG CGGCTGTTGC CCGCGTGGCG CTCGGCGCGG GCCGGGATCC ACGCGGTGTA TCCGTCGACG CGCATGCCGT CGCGGAAAGT GCGCGCGTTC GTCGACGCGA TGAAAGCGTG CATCGGAACC GCACGGCCAA CCGCACCGGC GCGCGCGACG GCGTCGAAAC GCGCGGCGCG GCGCGCGCCC GGAGCGGCT

Details for BuviA.12800.q.A1.GE32470

HARVESTED ON:	11/8/2011
SEQUENCED ON:	11/11/2011
EXPECTED MW:	36kDa
OBSERVED MW:	36kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with sequence variation
PERCENT IDENTITY:	data unavailable
PERCENT COVERAGE:	data unavailable

Validated AA Sequence

sequence unavailable

Validated NT Sequence

sequence unavailable

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMADLRDVNL NRLAIFVAVV DAGSLTAAAE RLGLAKTVVS THMQRLESEV GANLLVRTTR RLSVTDAGRA FYDACRDILR AAEAALDAAS SDAGPLRGTL RVSVPIDYGA LVVAPAIVAL RDAHPALDVE LIANDRVVDL VADNLDVAIR IGRLADSNYR AVQLGEYEKW LVASPAFVAR YGLPRDPDAL AALPFVMLST LPRPHTLELD HARGAHASVR CVAPVVSNTA TACRAIALAG GGFGLLTDFS IADDVAAGRL VRLLPAWRSA RAGIHAVYPS TRMPSRKVRA FVDAMKACIG TARPTAPARA TASKRAARRA PGAA

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggccgatcta cgcgacgtga acctgaaccg gctggcgatc ttcgtcgcgg tggtcgatgc cggctcgctg acggccgccg ccgagcggct cgggctggcg aagacggtcg tcagcacgca catgcagcgg ctcgaatccg aggtcggcgc gaacctgctg gtgcgcacca cgcggcgcct gagcgtgacc gacgcggggc gtgcgttcta cgatgcgtgc cgcgacatcc tgcgtgcggc ggaagccgcg ctcgacgccg cgtcgtccga cgcggggccg ctgcgcggca cgctgcgcgt gagcgtgccg atcgactacg gcgcgctggt ggtcgcgccg gcgatcgtcg cgttgcgcga cgcgcatccg gcgctcgacg tcgagctgat cgcgaacgac cgggtcgtcg atctcgtcgc ggacaacctc gacgtcgcga tccgcatcgg ccggctcgcc gattcgaact accgcgcagt gcaactcggc gaatacgaga aatggctggt ggcgagcccc gcgttcgtcg cgcgctacgg gctgccgcgc gatccggacg cgctcgccgc gctgccgttc gtgatgctgt cgacgctgcc gcgcccgcat acgctcgaac tcgaccatgc gcgcggcgct cacgcgtcgg tgcgctgcgt cgcgccggtc gtctcgaaca ccgcgaccgc gtgccgcgcg atcgcgctcg cgggcggcgg cttcgggctc ctgacggatt tctcgatcgc cgacgacgtg gcggccggcc ggctggtgcg gctgttgccc gcgtggcgct cggcgcgggc cgggatccac gcggtgtatc cgtcgacgcg catgccgtcg cggaaagtgc gcgcgttcgt cgacgcgatg aaagcgtgca tcggaaccgc acggccaacc gcaccggcgc gcgcgacggc gtcgaaacgc gcggcgcggc gcgcgcccgg agcggcttaa acagcacgaa caagttctgc agccaagctt ctcgaggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa