BuviA.12800.o

Transcriptional regulator, LysR family

CENTER ID: BuviA.12800.o
ORGANISM: Burkholderia vietnamiensis G4
ASSOCIATED DISEASE:
CURRENT STATUS: soluble
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BuviA.12800.o.A1.GE32468 Full length( BuviA.12800.o ) 1 348
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|269482.11.peg.2682
RefSeq: YP_001116399.1
UniProt: A4JKY1

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MDRTLHAMSV FSQVARLGSF TAAAEMLGIS AASASTLVRR LEEHLGATLL QRSTRCVRLT TEGEQYQEHC QRILGEIELM QQQLSGAGKI ARGRLAVDLD QEVAHSVLPF LPVFRANHPE VGLRVDVGGD ADGLIANGVD CAVVVGQLAD SSLRSRRVGH FHAVTVASPD YLKARGTPVD PDSLHCHDVI HYTPRRFGPV REFRYTVDGA ETRLKLAERI SVNDVRSAVR YAVEGVGIAQ VSERMVAEEL ARGTLVSILG EYGPAPLPIS VLYPDRRQVP MSIRAFIDWM QTQLRDLEIA SFDHTSAPNG GRDAAPYWTP NSERNNSERN NSERPFITAG FQPLSATA
NT Sequence
ATGGACCGTA CCCTGCATGC GATGTCAGTG TTCAGCCAGG TCGCGCGCCT GGGCAGCTTC ACCGCGGCGG CCGAGATGCT CGGCATCAGC GCGGCATCGG CCAGCACGCT GGTGCGCCGG CTCGAGGAGC ATCTCGGCGC AACGCTGCTG CAGCGCTCCA CGCGCTGCGT CCGGCTCACC ACGGAGGGCG AGCAGTATCA GGAGCACTGC CAGCGCATTC TCGGCGAGAT CGAGCTGATG CAGCAGCAAC TGAGCGGCGC CGGGAAGATC GCGCGCGGCC GGCTGGCGGT GGATCTCGAT CAGGAAGTCG CGCATTCGGT GTTGCCGTTC CTGCCGGTGT TCCGCGCGAA CCATCCGGAA GTGGGCTTGC GGGTCGACGT CGGCGGCGAC GCGGACGGCC TGATCGCGAA CGGCGTCGAC TGCGCGGTCG TGGTCGGCCA GCTGGCCGAC TCGTCGCTGC GCAGCCGGCG CGTCGGGCAC TTCCATGCGG TGACGGTGGC GAGCCCCGAC TATCTGAAGG CGCGCGGCAC GCCCGTCGAC CCCGACAGCC TCCATTGCCA CGACGTGATC CATTACACGC CGCGGCGCTT CGGTCCGGTC CGCGAGTTCC GTTACACGGT GGACGGCGCC GAGACGCGGC TCAAGCTGGC GGAGCGCATT TCCGTCAATG ACGTGCGTTC GGCCGTTCGA TACGCGGTCG AAGGCGTCGG CATCGCGCAG GTGAGCGAAC GGATGGTCGC GGAGGAGTTG GCGAGGGGCA CGCTCGTCAG CATTCTCGGC GAGTACGGCC CGGCGCCGCT GCCGATTTCG GTGCTGTATC CGGATCGACG CCAGGTGCCG ATGTCGATCC GCGCGTTCAT CGACTGGATG CAGACGCAGC TTCGCGATCT GGAAATCGCC AGCTTCGACC ACACGTCGGC GCCGAACGGC GGGCGCGACG CGGCGCCGTA CTGGACGCCG AACAGCGAGC GCAACAACAG CGAGCGCAAC AACAGCGAGC GGCCGTTCAT TACAGCGGGC TTCCAGCCGC TGTCGGCCAC GGCG
Details for BuviA.12800.o.A1.GE32468
HARVESTED ON: 11/8/2011
SEQUENCED ON: 11/11/2011
EXPECTED MW: 40kDa
OBSERVED MW: 40kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 100
PERCENT COVERAGE: 97
Validated AA Sequence
MGTLEAQTQG PGSMDRTLHA MSVFSQVARL GSFTAAAEML GISAASASTL VRRLEEHLGA TLLQRSTRCV RLTTEGEQYQ EHCQRILGEI ELMQQQLSGA GKIARGRLAV DLDQEVAHSV LPFLPVFRAN HPEVGLRVDV GGDADGLIAN GVDCAVVVGQ LADSSLRSRR VGHFHAVTVA SPDYLKARGT PVDPDSLHCH DVIHYTPRRF GPVREFRYTV DGAETRLKLA ERISVNDVRS AVRYAVEGVG IAQVSERMVA EELARGTLVS ILGEYGPAPL PISVLYPDRR QVPMSIRAFI DWMQTQLRDL EIASFDHTSA PNGGRDAAPY WTPNSERNNS ERNNSERPFI TAGFQPLSAT
Validated NT Sequence
cgccgtggcc gacagcggct ggaagcccgc tgtaatgaac ggccgctcgc tgttgttgcg ctcgctgttg ttgcgctcgc tgttcggcgt ccagtacggc gccgcgtcgc gcccgccgtt cggcgccgac gtgtggtcga agctggcgat ttccagatcg cgaagctgcg tctgcatcca gtcgatgaac gcgcggatcg acatcggcac ctggcgtcga tccggataca gcaccgaaat cggcagcggc gccgggccgt actcgccgag aatgctgacg agcgtgcccc tcgccaactc ctccgcgacc atccgttcgc tcacctgcgc gatgccgacg ccttcgaccg cgtatcgaac ggccgaacgc acgtcattga cggaaatgcg ctccgccagc ttgagccgcg tctcggcgcc gtccaccgtg taacggaact cgcggaccgg accgaagcgc cgcggcgtgt aatggatcac gtcgtggcaa tggaggctgt cggggtcgac gggcgtgccg cgcgccttca gatagtcggg gctcgccacc gtcaccgcat ggaagtgccc gacgcgccgg ctgcgcagcg acgagtcggc cagctggccg accacgaccg cgcagtcgac gccgttcgcg atcaggccgt ccgcgtcgcc gccgacgtcg acccgcaagc ccacttccgg atggttcgcg cggaacaccg gcaggaacgg caacaccgaa tgcgcgactt cctgatcgag atccaccgcc agccggccgc gcgcgatctt cccggcgccg ctcagttgct gctgcatcag ctcgatctcg ccgagaatgc gctggcagtg ctcctgatac tgctcgccct ccgtggtgag ccggacgcag cgcgtggagc gctgcagcag cgttgcgccg agatgctcct cgagccggcg caccagcgtg ctggccgatg ccgcgctgat gccgagcatc tcggccgccg cggtgaagct gcccaggcgc gcgacctggc tgaacactga catcgcatgc agggtacggt ccatcgaacc aggaccctgg gtctgagctt ccagggtacc catatgatgg tgatgg
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMDRTLHAMS VFSQVARLGS FTAAAEMLGI SAASASTLVR RLEEHLGATL LQRSTRCVRL TTEGEQYQEH CQRILGEIEL MQQQLSGAGK IARGRLAVDL DQEVAHSVLP FLPVFRANHP EVGLRVDVGG DADGLIANGV DCAVVVGQLA DSSLRSRRVG HFHAVTVASP DYLKARGTPV DPDSLHCHDV IHYTPRRFGP VREFRYTVDG AETRLKLAER ISVNDVRSAV RYAVEGVGIA QVSERMVAEE LARGTLVSIL GEYGPAPLPI SVLYPDRRQV PMSIRAFIDW MQTQLRDLEI ASFDHTSAPN GGRDAAPYWT PNSERNNSER NNSERPFITA GFQPLSATA
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggaccgtacc ctgcatgcga tgtcagtgtt cagccaggtc gcgcgcctgg gcagcttcac cgcggcggcc gagatgctcg gcatcagcgc ggcatcggcc agcacgctgg tgcgccggct cgaggagcat ctcggcgcaa cgctgctgca gcgctccacg cgctgcgtcc ggctcaccac ggagggcgag cagtatcagg agcactgcca gcgcattctc ggcgagatcg agctgatgca gcagcaactg agcggcgccg ggaagatcgc gcgcggccgg ctggcggtgg atctcgatca ggaagtcgcg cattcggtgt tgccgttcct gccggtgttc cgcgcgaacc atccggaagt gggcttgcgg gtcgacgtcg gcggcgacgc ggacggcctg atcgcgaacg gcgtcgactg cgcggtcgtg gtcggccagc tggccgactc gtcgctgcgc agccggcgcg tcgggcactt ccatgcggtg acggtggcga gccccgacta tctgaaggcg cgcggcacgc ccgtcgaccc cgacagcctc cattgccacg acgtgatcca ttacacgccg cggcgcttcg gtccggtccg cgagttccgt tacacggtgg acggcgccga gacgcggctc aagctggcgg agcgcatttc cgtcaatgac gtgcgttcgg ccgttcgata cgcggtcgaa ggcgtcggca tcgcgcaggt gagcgaacgg atggtcgcgg aggagttggc gaggggcacg ctcgtcagca ttctcggcga gtacggcccg gcgccgctgc cgatttcggt gctgtatccg gatcgacgcc aggtgccgat gtcgatccgc gcgttcatcg actggatgca gacgcagctt cgcgatctgg aaatcgccag cttcgaccac acgtcggcgc cgaacggcgg gcgcgacgcg gcgccgtact ggacgccgaa cagcgagcgc aacaacagcg agcgcaacaa cagcgagcgg ccgttcatta cagcgggctt ccagccgctg tcggccacgg cgtaaacagc acgaacaagt tctgcagcca agcttctcga ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaa