SSGCID: Target Page

BuviA.12800.m

Transcriptional regulator, LysR family

CENTER ID:	BuviA.12800.m
ORGANISM:	Burkholderia vietnamiensis G4
ASSOCIATED DISEASE:
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	True
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BuviA.12800.m.A1.GE32466	Full length( BuviA.12800.m )		1	307

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|269482.11.peg.2496
RefSeq:	YP_001116231.1
UniProt:	A4JKG3

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MNKSPNLDDL RVFSLVVRLT SFSAAAEQLA VSPAYVSKRI ALLEAQLGTR LLHRSTRRVT VTDAGERVFA RAEKILDDVD HLVEDVSTTR TVPRGTLRIS SSFGFGRHVV APALLDFSAR YPQLNVRLDL FDRLVDVVGE GYDVDVRIGD EIADHLIARR LAANYRVLCA SPEYLARRGT PRTLADLASH DCLAIKERDH PLGVWRLNVR GETATVKVGG ALSTNHGEVA VQWALAGRGI VLRSIWEAGP LIERGELRRV LPDAIQPANI WAVYPERVAA SAKVRVCVDF LADALAGLNA AAGNDAG

NT Sequence

GTGAACAAAT CACCGAACCT CGACGACCTG CGCGTGTTCA GCCTGGTCGT GCGCCTGACG AGCTTCAGCG CGGCCGCCGA GCAGCTCGCG GTGTCGCCCG CGTACGTGAG CAAGCGGATC GCGCTGCTCG AGGCGCAGCT CGGCACGCGC CTGCTGCATC GTTCGACGCG CCGCGTGACG GTCACGGACG CCGGTGAACG CGTGTTCGCG CGCGCCGAGA AGATTCTCGA CGACGTCGAT CATCTCGTCG AAGACGTGTC GACCACGCGC ACGGTGCCGC GCGGCACGCT GCGCATCTCG AGCAGCTTCG GGTTCGGGCG GCATGTCGTC GCGCCGGCGC TGCTCGATTT TTCCGCGCGC TATCCGCAGC TGAACGTGCG GCTCGATCTG TTCGACCGGC TCGTCGATGT CGTGGGCGAA GGCTACGACG TCGACGTGCG CATCGGCGAC GAGATCGCCG ATCACCTGAT CGCGCGCCGT CTCGCGGCGA ACTATCGCGT GCTGTGCGCG TCGCCCGAGT ACCTCGCGCG GCGCGGCACG CCGCGCACGC TCGCCGATCT CGCGTCGCAC GACTGTCTCG CCATCAAGGA GCGCGACCAT CCGCTCGGCG TGTGGCGGCT GAACGTGCGC GGCGAAACGG CGACGGTGAA GGTCGGCGGC GCGTTGTCGA CGAACCACGG CGAGGTGGCC GTGCAATGGG CGCTCGCCGG ACGCGGGATC GTGCTGCGTT CGATCTGGGA AGCGGGTCCG TTGATCGAGC GCGGCGAGTT GCGTCGCGTG CTGCCGGACG CAATTCAGCC GGCGAACATC TGGGCGGTGT ATCCGGAGCG TGTGGCGGCT TCCGCGAAGG TGCGCGTGTG CGTCGACTTT CTGGCGGATG CGCTCGCCGG GTTGAATGCC GCGGCGGGCA ACGATGCGGG C

Details for BuviA.12800.m.A1.GE32466

HARVESTED ON:	11/8/2011
SEQUENCED ON:	11/11/2011
EXPECTED MW:	35kDa
OBSERVED MW:	36kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	93
PERCENT COVERAGE:	106

Validated AA Sequence

MAHHHHHHMG TLEAQTQGPG SMVNKSPNLD DLRVFSLVVR LTSFSAAAEQ LAVSPAYVSK RIALLEAQLG TRLLHRSTRR VTVTDAGERV FARAEKILDD VDHLVEDVST TRTVPRGTLR ISSSFGFGRH VVAPALLDFS ARYPQLNVRL DLFDRLVDVV GEGYDVDVRI GDEIADHLIA RRLAANYRVL CASPEYLARR GTPRTLADLA SHDCLAIKER DHPLGVWRLN VRGETATVKV GGALSTNHGE VAVQWALAGR GIVLRSIWEA GPLIERGELR RVLPDAIQPA NIWAVYXGAC GGFXEXRVCV DFLADASPGE XXXXXRXXLX XSTXXCXXXX XXXXXXXXXX X

Validated NT Sequence

gaannntttt ntttnncttt aagaaggaga tataccatgg ctcatcacca tcaccatcat atgggtaccc tggaagctca gacccagggt cctggttcga tggtgaacaa atcaccgaac ctcgacgacc tgcgcgtgtt cagcctggtc gtgcgcctga cgagcttcag cgcggccgcc gagcagctcg cggtgtcgcc cgcgtacgtg agcaagcgga tcgcgctgct cgaggcgcag ctcggcacgc gcctgctgca tcgttcgacg cgccgcgtga cggtcacgga cgccggtgaa cgcgtgttcg cgcgcgccga gaagattctc gacgacgtcg atcatctcgt cgaagacgtg tcgaccacgc gcacggtgcc gcgcggcacg ctgcgcatct cgagcagctt cgggttcggg cggcatgtcg tcgcgccggc gctgctcgat ttttccgcgc gctatccgca gctgaacgtg cggctcgatc tgttcgaccg gctcgtcgat gtcgtgggcg aaggctacga cgtcgacgtg cgcatcggcg acgagatcgc cgatcacctg atcgcgcgcc gtctcgcggc gaactatcgc gtgctgtgcg cgtcgcccga gtacctcgcg cggcgcggca cgccgcgcac gctcgccgat ctcgcgtcgc acgactgtct cgccatcaag gagcgcgacc atccgctcgg cgtgtggcgg ctgaacgtgc gcggcgaaac ggcgacggtg aaggtcggcg gcgcgttgtc gacgaaccac ggcgaggtgg ccgtgcaatg ggcgctcgcc ggacgcggga tcgtgctgcg ttcgatctgg gaagcgggtc cgttgatcga gcgcggcgag ttgcgtcgcg tgctgccgga cgcaattcag ccggcgaaca tctgggcggt gtatncngga gcgtgtggcg gcttccncga anngcgcgtg tgcgtcgact ttctggcgga tgcctcgccg ggngaatgnn gcngnnggca ncgatgnngg ctnntaanca gcacnanann ntgcannnnn tnncnnngnn nnnntgnnnn cnaannnnaa nganctnant g

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggtgaacaaa tcaccgaacc tcgacgacct gcgcgtgttc agcctggtcg tgcgcctgac gagcttcagc gcggccgccg agcagctcgc ggtgtcgccc gcgtacgtga gcaagcggat cgcgctgctc gaggcgcagc tcggcacgcg cctgctgcat cgttcgacgc gccgcgtgac ggtcacggac gccggtgaac gcgtgttcgc gcgcgccgag aagattctcg acgacgtcga tcatctcgtc gaagacgtgt cgaccacgcg cacggtgccg cgcggcacgc tgcgcatctc gagcagcttc gggttcgggc ggcatgtcgt cgcgccggcg ctgctcgatt tttccgcgcg ctatccgcag ctgaacgtgc ggctcgatct gttcgaccgg ctcgtcgatg tcgtgggcga aggctacgac gtcgacgtgc gcatcggcga cgagatcgcc gatcacctga tcgcgcgccg tctcgcggcg aactatcgcg tgctgtgcgc gtcgcccgag tacctcgcgc ggcgcggcac gccgcgcacg ctcgccgatc tcgcgtcgca cgactgtctc gccatcaagg agcgcgacca tccgctcggc gtgtggcggc tgaacgtgcg cggcgaaacg gcgacggtga aggtcggcgg cgcgttgtcg acgaaccacg gcgaggtggc cgtgcaatgg gcgctcgccg gacgcgggat cgtgctgcgt tcgatctggg aagcgggtcc gttgatcgag cgcggcgagt tgcgtcgcgt gctgccggac gcaattcagc cggcgaacat ctgggcggtg tatccggagc gtgtggcggc ttccgcgaag gtgcgcgtgt gcgtcgactt tctggcggat gcgctcgccg ggttgaatgc cgcggcgggc aacgatgcgg gctaaacagc acgaacaagt tctgcagcca agcttctcga ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaa