BuviA.12800.l

Transcriptional regulator, LysR family

CENTER ID: BuviA.12800.l
ORGANISM: Burkholderia vietnamiensis G4
ASSOCIATED DISEASE:
CURRENT STATUS: soluble
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BuviA.12800.l.A1.GE32465 Full length( BuviA.12800.l ) 1 305
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|269482.11.peg.2079
RefSeq: YP_001115851.1
UniProt: A4JJD3

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MARLPDFEGL AMFAKVAEEG SFAAAARSMG VSVATVSRGV ARLEGRLGGR LFNRTSRQLA LTEFGRTLCE KAGDMYRHAE EAESAAREMA VQPRGLVRLA VPMSFGLRWV APLLPAFFRA YPEVSIDLHL SDASVDLIAD GFDAALRIAA LPDSSLVARR LCAVTQYVVA SPAYLAREGR PTHPRDLAGR ACLSYAYRAH SDVWRFTNDA GEEEPILPNG PLRVTNSDAL LPTLLEGLAI GELPEFIAGE YLADGRLEAI LTDWSLTKGG LYFITPSARA RPAKIAALSD FFAEHLSEPS WRWPR
NT Sequence
ATGGCCAGAT TGCCTGACTT CGAGGGGCTC GCAATGTTCG CGAAGGTCGC CGAGGAGGGC TCGTTCGCGG CGGCGGCCCG CTCGATGGGC GTGTCGGTGG CGACCGTGTC GCGCGGCGTC GCGCGCCTGG AAGGCCGGCT CGGCGGGCGG CTCTTCAACC GCACGTCGCG GCAGCTCGCG CTCACCGAGT TCGGCCGGAC CTTGTGCGAA AAGGCGGGCG ACATGTATCG CCACGCGGAA GAAGCGGAGA GCGCCGCGCG TGAGATGGCC GTGCAGCCGC GCGGGCTCGT GCGGCTCGCG GTGCCGATGT CGTTCGGCCT GCGTTGGGTC GCGCCGCTGT TGCCGGCGTT CTTTCGCGCC TATCCGGAAG TGTCGATCGA CCTGCATCTG TCGGATGCGT CGGTCGATCT GATCGCCGAC GGTTTCGATG CCGCGCTGCG GATCGCCGCG CTGCCCGATT CGTCGCTGGT CGCGCGGCGC CTGTGCGCGG TGACGCAATA CGTGGTCGCG TCGCCGGCCT ATCTGGCGCG CGAAGGCCGG CCGACGCATC CGCGCGATCT GGCCGGGCGC GCATGCCTGT CGTATGCGTA CCGCGCGCAC AGCGACGTGT GGCGCTTCAC GAACGACGCT GGCGAAGAAG AGCCGATCCT GCCGAACGGG CCGCTGCGCG TGACGAATTC CGACGCGCTG CTGCCGACGC TGCTCGAAGG GCTCGCGATC GGCGAGCTGC CGGAGTTCAT CGCCGGCGAA TATCTGGCCG ACGGCCGGCT CGAAGCGATC CTGACCGACT GGTCGCTCAC CAAGGGCGGC CTGTACTTCA TCACGCCTTC CGCGCGCGCC CGACCGGCGA AGATCGCCGC GCTGTCCGAC TTCTTCGCCG AACATCTGTC GGAGCCGAGC TGGCGGTGGC CGAGG
Details for BuviA.12800.l.A1.GE32465
HARVESTED ON: 11/8/2011
SEQUENCED ON: 11/11/2011
EXPECTED MW: 35kDa
OBSERVED MW: 36kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Few (1-10)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 94
PERCENT COVERAGE: 86
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMARLPDFEG LAMFAKVAEE GSFAAAARSM GVSVATVSRG VARLEGRLGG RLFNRTSRQL ALTEFGRTLC EKAGDMYRHA EEAESAAREM AVQPRGLVRL AVPMSFGLRW VAPLLPAFFR AYPEVSIDLH LSDASVDLIA DGFDAALRIA ALPDSSLVAR RLCAVTQYVV ASPAYLAREG RPTHPRDLAG RACLSYAYRA HSDVWRFTND AGEEEPILPN GPLRVTXSDA LLPTLLEXLA IGELPDSSPA NIWXTAGSKR S
Validated NT Sequence
ttgtttaact ttaagaagga gatataccat ggctcatcac catcaccatc atatgggtac cctggaagct cagacccagg gtcctggttc gatggccaga ttgcctgact tcgaggggct cgcaatgttc gcgaaggtcg ccgaggaggg ctcgttcgcg gcggcggccc gctcgatggg cgtgtcggtg gcgaccgtgt cgcgcggcgt cgcgcgcctg gaaggccggc tcggcgggcg gctcttcaac cgcacgtcgc ggcagctcgc gctcaccgag ttcggccgga ccttgtgcga aaaggcgggc gacatgtatc gccacgcgga agaagcggag agcgccgcgc gtgagatggc cgtgcagccg cgcgggctcg tgcggctcgc ggtgccgatg tcgttcggcc tgcgttgggt cgcgccgctg ttgccggcgt tctttcgcgc ctatccggaa gtgtcgatcg acctgcatct gtcggatgcg tcggtcgatc tgatcgccga cggtttcgat gccgcgctgc ggatcgccgc gctgcccgat tcgtcgctgg tcgcgcggcg cctgtgcgcg gtgacgcaat acgtggtcgc gtcgccggcc tatctggcgc gcgaaggccg gccgacgcat ccgcgcgatc tggccgggcg cgcatgcctg tcgtatgcgt accgcgcgca cagcgacgtg tggcgcttca cgaacgacgc tggcgaagaa gagccgatcc tgccgaacgg gccgctgcgc gtgacgaant ccgacgcgct gctgccgacg ctgctcgaan ggctcgcgat cggcgagctg ccngattcat cgccggcgaa tatctggncg acggccggct cgaagcgatc ctgaccgact ggtcgctcac caagggcggn ctgtacttca tcacgccttc cgcgcgcgcc cgaccggcga agatcgccnc gctgtccgan ttcntcgccn aacatctgtc gganccganc tggcggnngg ccnaggtant nannngcang annntntgca nnnnctnncn angnnnnntg cnnnnaannc cgaanggnag ctgatgntg
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMARLPDFEG LAMFAKVAEE GSFAAAARSM GVSVATVSRG VARLEGRLGG RLFNRTSRQL ALTEFGRTLC EKAGDMYRHA EEAESAAREM AVQPRGLVRL AVPMSFGLRW VAPLLPAFFR AYPEVSIDLH LSDASVDLIA DGFDAALRIA ALPDSSLVAR RLCAVTQYVV ASPAYLAREG RPTHPRDLAG RACLSYAYRA HSDVWRFTND AGEEEPILPN GPLRVTNSDA LLPTLLEGLA IGELPEFIAG EYLADGRLEA ILTDWSLTKG GLYFITPSAR ARPAKIAALS DFFAEHLSEP SWRWPR
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggccagattg cctgacttcg aggggctcgc aatgttcgcg aaggtcgccg aggagggctc gttcgcggcg gcggcccgct cgatgggcgt gtcggtggcg accgtgtcgc gcggcgtcgc gcgcctggaa ggccggctcg gcgggcggct cttcaaccgc acgtcgcggc agctcgcgct caccgagttc ggccggacct tgtgcgaaaa ggcgggcgac atgtatcgcc acgcggaaga agcggagagc gccgcgcgtg agatggccgt gcagccgcgc gggctcgtgc ggctcgcggt gccgatgtcg ttcggcctgc gttgggtcgc gccgctgttg ccggcgttct ttcgcgccta tccggaagtg tcgatcgacc tgcatctgtc ggatgcgtcg gtcgatctga tcgccgacgg tttcgatgcc gcgctgcgga tcgccgcgct gcccgattcg tcgctggtcg cgcggcgcct gtgcgcggtg acgcaatacg tggtcgcgtc gccggcctat ctggcgcgcg aaggccggcc gacgcatccg cgcgatctgg ccgggcgcgc atgcctgtcg tatgcgtacc gcgcgcacag cgacgtgtgg cgcttcacga acgacgctgg cgaagaagag ccgatcctgc cgaacgggcc gctgcgcgtg acgaattccg acgcgctgct gccgacgctg ctcgaagggc tcgcgatcgg cgagctgccg gagttcatcg ccggcgaata tctggccgac ggccggctcg aagcgatcct gaccgactgg tcgctcacca agggcggcct gtacttcatc acgccttccg cgcgcgcccg accggcgaag atcgccgcgc tgtccgactt cttcgccgaa catctgtcgg agccgagctg gcggtggccg aggtaaacag cacgaacaag ttctgcagcc aagcttctcg aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaa