SSGCID: Target Page

BuviA.12800.j

Transcriptional regulator, LysR family

CENTER ID:	BuviA.12800.j
ORGANISM:	Burkholderia vietnamiensis G4
ASSOCIATED DISEASE:
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	True
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BuviA.12800.j.A1.GE32463	Full length( BuviA.12800.j )		1	319

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|269482.11.peg.7327
RefSeq:	YP_001120881.1
UniProt:	A4JIE3

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MDTLVSMNVF RYVVEVGSFV GAAERMQMSA AMASKHVMHL EQQLGARLLH RTTRRVAPTE AGREYYERLV QALSELDEAG QAVGAASVVP QGRLRVTSLS AFGLRHVMQA VTDYAGRFPD VTVDMTLSDR VVDLIEEGYD VAVRAAPNGL KSSSLVARQI ATAHILLVAS PDYLERRGTP QTIADLAYHN YLRRESSASA LDALVIDAAA ASRVNLNGNL IVNHLEGLRA AVLAGAGIAL LGTEVVGDDI ESGRLVPLLL DAVPPHEAPI YAVYASRRHV SAKVRSFVDF LAARFEGQSL CPSIEARLRV LPITRIKRA

NT Sequence

ATGGATACCC TCGTCAGCAT GAATGTGTTT CGGTACGTCG TCGAAGTAGG CAGCTTCGTG GGCGCGGCCG AGCGCATGCA GATGTCGGCC GCGATGGCGA GCAAGCACGT GATGCATCTC GAGCAGCAGC TCGGCGCGCG GCTGCTGCAT CGTACGACAC GACGCGTCGC GCCGACCGAG GCGGGACGCG AATACTACGA GCGCCTCGTG CAGGCGCTGT CGGAACTCGA CGAGGCAGGG CAGGCGGTGG GCGCCGCGAG CGTCGTGCCG CAGGGGCGGT TGCGCGTGAC GTCGCTGTCG GCGTTCGGCT TGCGGCACGT GATGCAGGCC GTCACCGACT ATGCGGGCCG GTTCCCGGAC GTGACCGTCG ACATGACGCT GTCCGACCGT GTCGTCGATC TGATCGAGGA AGGCTATGAC GTCGCGGTGC GCGCGGCGCC GAACGGATTG AAGTCGTCGT CGCTGGTCGC GCGGCAGATC GCGACCGCGC ACATTCTGCT GGTCGCGTCG CCCGACTATC TGGAGCGGCG CGGCACGCCG CAGACGATCG CCGATCTCGC GTACCACAAC TATCTGCGGC GCGAGTCGAG CGCCTCGGCG CTCGATGCGC TGGTGATCGA CGCGGCCGCC GCGTCGCGCG TGAACCTGAA CGGCAACCTG ATCGTGAACC ATCTCGAAGG GCTGCGCGCG GCCGTGCTCG CGGGCGCGGG CATCGCGCTG CTGGGCACCG AGGTGGTCGG CGACGATATC GAATCCGGCC GGCTCGTGCC GCTGCTGCTC GACGCGGTGC CGCCGCACGA GGCGCCGATC TATGCGGTGT ACGCGAGCCG CCGTCACGTA TCGGCGAAGG TGCGCTCGTT CGTCGATTTC CTCGCCGCGC GCTTCGAAGG GCAGTCGCTG TGCCCGTCGA TCGAGGCGCG GCTGCGCGTG CTGCCGATCA CGCGAATCAA GCGAGCT

Details for BuviA.12800.j.A1.GE32463

HARVESTED ON:	11/8/2011
SEQUENCED ON:	11/11/2011
EXPECTED MW:	36kDa
OBSERVED MW:	37kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	100
PERCENT COVERAGE:	93

Validated AA Sequence

Validated NT Sequence

ttattaagct cgcttgattc gcgtgatcgg cagcacgcgc agccgcgcct cgatcgacgg gcacagcgac tgcccttcga agcgcgcggc gaggaaatcg acgaacgagc gcaccttcgc cgatacgtga cggcggctcg cgtacaccgc atagatcggc gcctcgtgcg gcggcaccgc gtcgagcagc agcggcacga gccggccgga ttcgatatcg tcgccgacca cctcggtgcc cagcagcgcg atgcccgcgc ccgcgagcac ggccgcgcgc agcccttcga gatggttcac gatcaggttg ccgttcaggt tcacgcgcga cgcggcggcc gcgtcgatca ccagcgcatc gagcgccgag gcgctcgact cgcgccgcag atagttgtgg tacgcgagat cggcgatcgt ctgcggcgtg ccgcgccgct ccagatagtc gggcgacgcg accagcagaa tgtgcgcggt cgcgatctgc cgcgcgacca gcgacgacga cttcaatccg ttcggcgccg cgcgcaccgc gacgtcatag ccttcctcga tcagatcgac gacacggtcg gacagcgtca tgtcgacggt cacgtccggg aaccggcccg catagtcggt gacggcctgc atcacgtgcc gcaagccgaa cgccgacagc gacgtcacgc gcaaccgccc ctgcggcacg acgctcgcgg cgcccaccgc ctgccctgcc tcgtcgagtt ccgacagcgc ctgcacgagg cgctcgtagt attcgcgtcc cgcctcggtc ggcgcgacgc gtcgtgtcgt acgatgcagc agccgcgcgc cgagctgctg ctcgagatgc atcacgtgct tgctcgccat cgcggccgac atctgcatgc gctcggccgc gcccacgaag ctgcctactt cgacgacgta ccgaaacaca ttcatgctga cgagggtatc catcgaacca ggaccctggg tctgagcttc cagggtacc

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMDTLVSMNV FRYVVEVGSF VGAAERMQMS AAMASKHVMH LEQQLGARLL HRTTRRVAPT EAGREYYERL VQALSELDEA GQAVGAASVV PQGRLRVTSL SAFGLRHVMQ AVTDYAGRFP DVTVDMTLSD RVVDLIEEGY DVAVRAAPNG LKSSSLVARQ IATAHILLVA SPDYLERRGT PQTIADLAYH NYLRRESSAS ALDALVIDAA AASRVNLNGN LIVNHLEGLR AAVLAGAGIA LLGTEVVGDD IESGRLVPLL LDAVPPHEAP IYAVYASRRH VSAKVRSFVD FLAARFEGQS LCPSIEARLR VLPITRIKRA

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggataccctc gtcagcatga atgtgtttcg gtacgtcgtc gaagtaggca gcttcgtggg cgcggccgag cgcatgcaga tgtcggccgc gatggcgagc aagcacgtga tgcatctcga gcagcagctc ggcgcgcggc tgctgcatcg tacgacacga cgcgtcgcgc cgaccgaggc gggacgcgaa tactacgagc gcctcgtgca ggcgctgtcg gaactcgacg aggcagggca ggcggtgggc gccgcgagcg tcgtgccgca ggggcggttg cgcgtgacgt cgctgtcggc gttcggcttg cggcacgtga tgcaggccgt caccgactat gcgggccggt tcccggacgt gaccgtcgac atgacgctgt ccgaccgtgt cgtcgatctg atcgaggaag gctatgacgt cgcggtgcgc gcggcgccga acggattgaa gtcgtcgtcg ctggtcgcgc ggcagatcgc gaccgcgcac attctgctgg tcgcgtcgcc cgactatctg gagcggcgcg gcacgccgca gacgatcgcc gatctcgcgt accacaacta tctgcggcgc gagtcgagcg cctcggcgct cgatgcgctg gtgatcgacg cggccgccgc gtcgcgcgtg aacctgaacg gcaacctgat cgtgaaccat ctcgaagggc tgcgcgcggc cgtgctcgcg ggcgcgggca tcgcgctgct gggcaccgag gtggtcggcg acgatatcga atccggccgg ctcgtgccgc tgctgctcga cgcggtgccg ccgcacgagg cgccgatcta tgcggtgtac gcgagccgcc gtcacgtatc ggcgaaggtg cgctcgttcg tcgatttcct cgccgcgcgc ttcgaagggc agtcgctgtg cccgtcgatc gaggcgcggc tgcgcgtgct gccgatcacg cgaatcaagc gagcttaaac agcacgaaca agttctgcag ccaagcttct cgaggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaa