BuviA.12800.g

Transcriptional regulator, LysR family

CENTER ID: BuviA.12800.g
ORGANISM: Burkholderia vietnamiensis G4
ASSOCIATED DISEASE:
CURRENT STATUS: soluble
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BuviA.12800.g.A1.GE32460 Full length( BuviA.12800.g ) 1 326
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|269482.11.peg.6527
RefSeq: YP_001120121.1
UniProt: A4JG83

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MNIDAHNLND PMYFSQVVEH GGFSAAERVL GISKSRLSRR LTELEAALGV RLLQRSTRKL ALTEAGELFY QHCQAMLAEA QAAMNAVQQL RASPRGSVRV SVPVTLSQTM LSRILPEFLH RYPEVKVHIR VTNRVIDLFE DSIDVALRVR SEPPQNANIV VRPLWRTEQM LVGAPSLLSQ HAPPLVPADL ARFETLDTPS VDGRHVFTLI APDGTRHLHE HDPRLVTADL MTIREAVLDG LGIAALPEMM YGNALRAGQL SPVMPGWTLP VPQLYAVFVS RQGMPPAVRA FVDYLVEKLD HGEYKQPGCP ERAKQEAAAA AAVTAQ
NT Sequence
ATGAACATCG ATGCACACAA CCTGAACGAC CCCATGTACT TCTCGCAGGT CGTCGAGCAT GGCGGCTTCT CGGCCGCGGA ACGCGTGCTC GGTATCTCCA AATCGCGCCT GTCGCGACGG CTGACGGAAC TCGAGGCCGC GCTCGGCGTG CGGCTGCTGC AGCGTTCGAC GCGCAAGCTC GCGCTGACCG AGGCCGGCGA GCTGTTCTAC CAGCATTGTC AGGCCATGCT GGCCGAAGCG CAGGCGGCGA TGAACGCGGT GCAGCAACTG CGCGCGTCGC CGCGCGGGTC GGTGCGCGTG AGCGTGCCCG TCACGCTGTC GCAGACGATG CTGTCGCGCA TCCTCCCCGA ATTCCTGCAC CGCTATCCGG AGGTGAAGGT GCACATCCGC GTGACGAATC GCGTGATCGA CCTGTTCGAG GATTCGATCG ACGTCGCGTT GCGCGTGCGC TCGGAGCCGC CGCAGAACGC GAATATCGTC GTGCGGCCGT TGTGGCGCAC CGAGCAGATG CTGGTCGGCG CGCCGAGCCT GCTGAGCCAG CATGCGCCGC CTCTCGTGCC CGCCGATCTC GCGCGCTTCG AGACGCTCGA TACGCCGAGC GTGGACGGGC GGCACGTGTT CACGCTGATC GCGCCGGACG GCACGCGCCA CCTGCACGAA CACGACCCGC GGCTCGTCAC GGCCGACCTG ATGACGATCC GCGAGGCCGT GCTCGACGGC CTCGGCATCG CGGCGTTGCC CGAGATGATG TACGGCAATG CGTTGCGCGC CGGGCAGCTG TCGCCGGTGA TGCCGGGCTG GACGCTGCCG GTGCCGCAAC TCTATGCGGT GTTCGTGTCG CGGCAGGGCA TGCCGCCGGC GGTGCGCGCT TTCGTCGACT ACCTGGTGGA GAAGCTCGAC CACGGCGAAT ACAAGCAGCC CGGTTGCCCC GAACGTGCGA AGCAGGAAGC GGCAGCGGCG GCGGCCGTTA CGGCCCAG
Details for BuviA.12800.g.A1.GE32460
HARVESTED ON: 11/8/2011
SEQUENCED ON: 11/11/2011
EXPECTED MW: 38kDa
OBSERVED MW: 37kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 88
PERCENT COVERAGE: 105
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMNIDAHNLN DPMYFSQVVE HGGFSAAERV LGISKSRLSR RLTELEAALG VRLLQRSTRK LALTEAGELF YQHCQAMLAE AQAAMNAVQQ LRASPRGSVR VSVPVTLSQT MLSRILPEFL HRYPEVKVHI RVTNRVIDLF EDSIDVALRV RSEPPQNANI VVRPLWRTEQ MLVGAPSLLS QHAPPLVPAD LARFETLDTP SVDGRHVFTL IAPDGTRHLH EHDPRLVTAD LMTIREAVLD GLGIAALPEM MYGNALRAGQ LSPVMPGWTL PVPQLYAVFV SRXXCRRRCA LSSTTWXXXS TXXXXQPXXX XCXXXXXXXX XXIXXXXSXX XXXXXXXXXX XXXXX
Validated NT Sequence
ttttgtttnn ctttaagaag gagatatacc atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgaaca tcgatgcaca caacctgaac gaccccatgt acttctcgca ggtcgtcgag catggcggct tctcggccgc ggaacgcgtg ctcggtatct ccaaatcgcg cctgtcgcga cggctgacgg aactcgaggc cgcgctcggc gtgcggctgc tgcagcgttc gacgcgcaag ctcgcgctga ccgaggccgg cgagctgttc taccagcatt gtcaggccat gctggccgaa gcgcaggcgg cgatgaacgc ggtgcagcaa ctgcgcgcgt cgccgcgcgg gtcggtgcgc gtgagcgtgc ccgtcacgct gtcgcagacg atgctgtcgc gcatcctccc cgaattcctg caccgctatc cggaggtgaa ggtgcacatc cgcgtgacga atcgcgtgat cgacctgttc gaggattcga tcgacgtcgc gttgcgcgtg cgctcggagc cgccgcagaa cgcgaatatc gtcgtgcggc cgttgtggcg caccgagcag atgctggtcg gcgcgccgag cctgctgagc cagcatgcgc cgcctctcgt gcccgccgat ctcgcgcgct tcgagacgct cgatacgccg agcgtggacg ggcggcacgt gttcacgctg atcgcgccgg acggcacgcg ccacctgcac gaacacgacc cgcggctcgt cacggccgac ctgatgacga tccgcgaggc cgtgctcgac ggcctcggca tcgcggcgtt gcccgagatg atgtacggca atgcgttgcg cgccgggcag ctgtcgccgg tgatgccggg ctggacgctg ccggtgccgc aactctatgc ggtgttcgtg tcgcggcnng natgccgccg gcggtgcgcg ctttcgtcga ctacctggnn gannanctcg acncngnnan tanngcagcc nnnnnnnnnn nngtgcnann nnnncgnnnc gnnnnnnnnn gnnnntataa nnnnnnnana ntcnnnntnn nnngnncnnn gctannancn nannnnnnnn ngnnnnnnnn nnnnnna
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMNIDAHNLN DPMYFSQVVE HGGFSAAERV LGISKSRLSR RLTELEAALG VRLLQRSTRK LALTEAGELF YQHCQAMLAE AQAAMNAVQQ LRASPRGSVR VSVPVTLSQT MLSRILPEFL HRYPEVKVHI RVTNRVIDLF EDSIDVALRV RSEPPQNANI VVRPLWRTEQ MLVGAPSLLS QHAPPLVPAD LARFETLDTP SVDGRHVFTL IAPDGTRHLH EHDPRLVTAD LMTIREAVLD GLGIAALPEM MYGNALRAGQ LSPVMPGWTL PVPQLYAVFV SRQGMPPAVR AFVDYLVEKL DHGEYKQPGC PERAKQEAAA AAAVTAQ
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gaacatcgat gcacacaacc tgaacgaccc catgtacttc tcgcaggtcg tcgagcatgg cggcttctcg gccgcggaac gcgtgctcgg tatctccaaa tcgcgcctgt cgcgacggct gacggaactc gaggccgcgc tcggcgtgcg gctgctgcag cgttcgacgc gcaagctcgc gctgaccgag gccggcgagc tgttctacca gcattgtcag gccatgctgg ccgaagcgca ggcggcgatg aacgcggtgc agcaactgcg cgcgtcgccg cgcgggtcgg tgcgcgtgag cgtgcccgtc acgctgtcgc agacgatgct gtcgcgcatc ctccccgaat tcctgcaccg ctatccggag gtgaaggtgc acatccgcgt gacgaatcgc gtgatcgacc tgttcgagga ttcgatcgac gtcgcgttgc gcgtgcgctc ggagccgccg cagaacgcga atatcgtcgt gcggccgttg tggcgcaccg agcagatgct ggtcggcgcg ccgagcctgc tgagccagca tgcgccgcct ctcgtgcccg ccgatctcgc gcgcttcgag acgctcgata cgccgagcgt ggacgggcgg cacgtgttca cgctgatcgc gccggacggc acgcgccacc tgcacgaaca cgacccgcgg ctcgtcacgg ccgacctgat gacgatccgc gaggccgtgc tcgacggcct cggcatcgcg gcgttgcccg agatgatgta cggcaatgcg ttgcgcgccg ggcagctgtc gccggtgatg ccgggctgga cgctgccggt gccgcaactc tatgcggtgt tcgtgtcgcg gcagggcatg ccgccggcgg tgcgcgcttt cgtcgactac ctggtggaga agctcgacca cggcgaatac aagcagcccg gttgccccga acgtgcgaag caggaagcgg cagcggcggc ggccgttacg gcccagtaaa cagcacgaac aagttctgca gccaagcttc tcgaggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaa