BuviA.12800.b

Transcriptional regulator, LysR family

CENTER ID: BuviA.12800.b
ORGANISM: Burkholderia vietnamiensis G4
ASSOCIATED DISEASE:
CURRENT STATUS: soluble
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BuviA.12800.b.A1.GE32455 Full length( BuviA.12800.b ) 1 313
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|269482.11.peg.5644
RefSeq: YP_001119255.1
UniProt: A4JDR7

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MDTLQNMRVF VRVVDAGSFT AAAQQMNSTT AYASRAVSDL EAHLRTRLLN RTTRRIALTE AGERYLQRCE QILAYVDQAE AEAGDAHARP SGKLKVHCFT SLGQHYLVPA IGRYRQRYPD VHVELTLAQR MPDLLDEGYD VAIVVGRELP DSGLVSQRLG DSYSVICASP DYVGAHGKPQ TPADLAQHAC LGMVAPGFHF DEWTLAGPRG DELVPVTAPP FRVNVAEALA VAVREGMGIG GLPLYSAIGG LRSGQIVRLL PEYRSQPMSI YALYPSRQYL DAKIRTWVDF LRDELPATLE ADEAALAQIT AAT
NT Sequence
ATGGATACGT TACAAAACAT GCGGGTATTC GTCCGCGTGG TGGATGCGGG GAGCTTTACC GCGGCCGCCC AGCAGATGAA TTCGACGACC GCCTATGCAT CGCGCGCCGT ATCGGATCTC GAAGCGCACC TGCGCACGCG CCTGCTGAAC CGGACCACGC GCCGGATCGC GCTGACCGAA GCCGGTGAGC GCTACCTGCA GCGCTGCGAG CAGATCCTCG CGTACGTCGA CCAGGCCGAA GCCGAGGCGG GCGATGCGCA CGCGCGCCCG TCGGGCAAGC TGAAGGTCCA TTGCTTCACG AGCCTCGGCC AGCACTATCT GGTGCCGGCC ATCGGGCGCT ACCGGCAGCG CTATCCGGAC GTGCACGTCG AGCTGACGCT CGCGCAGCGG ATGCCCGACC TGCTCGACGA AGGCTACGAC GTCGCGATCG TCGTGGGCCG CGAGCTGCCC GATTCGGGGC TCGTGTCGCA GCGGCTCGGC GACAGCTACA GCGTCATCTG CGCGTCGCCC GATTACGTCG GCGCGCACGG CAAGCCGCAG ACGCCGGCCG ATCTCGCGCA GCACGCCTGT CTCGGGATGG TCGCGCCGGG GTTCCACTTC GACGAATGGA CGCTGGCGGG GCCGCGCGGC GACGAGCTCG TGCCGGTGAC GGCGCCGCCG TTCCGCGTAA ATGTGGCCGA GGCGCTGGCG GTGGCCGTGC GGGAAGGGAT GGGGATCGGC GGCCTGCCGC TGTATTCGGC GATCGGCGGG CTGCGCAGCG GCCAGATCGT GCGCCTGCTG CCGGAATACC GGTCGCAGCC GATGAGCATC TACGCGCTGT ATCCGTCGCG CCAGTACCTC GACGCGAAAA TCCGGACGTG GGTCGATTTT CTGCGCGACG AACTGCCGGC TACGCTCGAA GCCGACGAAG CCGCGCTCGC GCAGATCACG GCTGCGACA
Details for BuviA.12800.b.A1.GE32455
HARVESTED ON: 11/8/2011
SEQUENCED ON: 11/11/2011
EXPECTED MW: 36kDa
OBSERVED MW: 36kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 45
PERCENT COVERAGE: 98
Validated AA Sequence
MGTLEAQTQG PGSMDTLQNM RVFVRVVDAG SFTAAAQQMN STTAYASRAV SDLEAHLRTR LLNRTTRRIA LTEAGERYLQ RCEQILAYVD QAEAEAGDAH ARPVGQAEGP LLHEPRPALS GAGHRALPAA LSGRARRADA RAADARPARR RLRRRDRRGP RAARFGARVA AARRQLQRHL RVARLRRRAR QAADAGRSRA ARLSRDGRAG VPLRRMDAGG AARRRARAGD GAAVPRKCGR GAGGGRAGRD GDRRPAAVFG DRRAAQRPDR APAAGIPVAA DEHLRAVSVA PVPRRENPDV GRFSARRTAG YARSRRSRAR ADHGCDII
Validated NT Sequence
ttattatgtc gcagccgtga tctgcgcgag cgcggcttcg tcggcttcga gcgtagccgg cagttcgtcg cgcagaaaat cgacccacgt ccggattttc gcgtcgaggt actggcgcga cggatacagc gcgtagatgc tcatcggctg cgaccggtat tccggcagca ggcgcacgat ctggccgctg cgcagcccgc cgatcgccga atacagcggc aggccgccga tccccatccc ttcccgcacg gccaccgcca gcgcctcggc cacatttacg cggaacggcg gcgccgtcac cggcacgagc tcgtcgccgc gcggccccgc cagcgtccat tcgtcgaagt ggaaccccgg cgcgaccatc ccgagacagg cgtgctgcgc gagatcggcc ggcgtctgcg gcttgccgtg cgcgccgacg taatcgggcg acgcgcagat gacgctgtag ctgtcgccga gccgctgcga cacgagcccc gaatcgggca gctcgcggcc cacgacgatc gcgacgtcgt agccttcgtc gagcaggtcg ggcatccgct gcgcgagcgt cagctcgacg tgcacgtccg gatagcgctg ccggtagcgc ccgatggccg gcaccagata gtgctggccg aggctcgtga agcaatggac cttcagcttg cccgacgggg cgcgcgtgcg catcgcccgc ctcggcttcg gcctggtcga cgtacgcgag gatctgctcg cagcgctgca ggtagcgctc accggcttcg gtcagcgcga tccggcgcgt ggtccggttc agcaggcgcg tgcgcaggtg cgcttcgaga tccgatacgg cgcgcgatgc ataggcggtc gtcgaattca tctgctgggc ggccgcggta aagctccccg catccaccac gcggacgaat acccgcatgt tttgtaacgt atccatcgaa ccaggaccct gggtctgagc ttccagggta cccatatgat gg
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMDTLQNMRV FVRVVDAGSF TAAAQQMNST TAYASRAVSD LEAHLRTRLL NRTTRRIALT EAGERYLQRC EQILAYVDQA EAEAGDAHAR PSGKLKVHCF TSLGQHYLVP AIGRYRQRYP DVHVELTLAQ RMPDLLDEGY DVAIVVGREL PDSGLVSQRL GDSYSVICAS PDYVGAHGKP QTPADLAQHA CLGMVAPGFH FDEWTLAGPR GDELVPVTAP PFRVNVAEAL AVAVREGMGI GGLPLYSAIG GLRSGQIVRL LPEYRSQPMS IYALYPSRQY LDAKIRTWVD FLRDELPATL EADEAALAQI TAAT
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggatacgtta caaaacatgc gggtattcgt ccgcgtggtg gatgcgggga gctttaccgc ggccgcccag cagatgaatt cgacgaccgc ctatgcatcg cgcgccgtat cggatctcga agcgcacctg cgcacgcgcc tgctgaaccg gaccacgcgc cggatcgcgc tgaccgaagc cggtgagcgc tacctgcagc gctgcgagca gatcctcgcg tacgtcgacc aggccgaagc cgaggcgggc gatgcgcacg cgcgcccgtc gggcaagctg aaggtccatt gcttcacgag cctcggccag cactatctgg tgccggccat cgggcgctac cggcagcgct atccggacgt gcacgtcgag ctgacgctcg cgcagcggat gcccgacctg ctcgacgaag gctacgacgt cgcgatcgtc gtgggccgcg agctgcccga ttcggggctc gtgtcgcagc ggctcggcga cagctacagc gtcatctgcg cgtcgcccga ttacgtcggc gcgcacggca agccgcagac gccggccgat ctcgcgcagc acgcctgtct cgggatggtc gcgccggggt tccacttcga cgaatggacg ctggcggggc cgcgcggcga cgagctcgtg ccggtgacgg cgccgccgtt ccgcgtaaat gtggccgagg cgctggcggt ggccgtgcgg gaagggatgg ggatcggcgg cctgccgctg tattcggcga tcggcgggct gcgcagcggc cagatcgtgc gcctgctgcc ggaataccgg tcgcagccga tgagcatcta cgcgctgtat ccgtcgcgcc agtacctcga cgcgaaaatc cggacgtggg tcgattttct gcgcgacgaa ctgccggcta cgctcgaagc cgacgaagcc gcgctcgcgc agatcacggc tgcgacataa acagcacgaa caagttctgc agccaagctt ctcgaggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa