BuviA.12797.a
ROK family protein
| CENTER ID: | BuviA.12797.a |
| ORGANISM: | Burkholderia vietnamiensis G4 |
| ASSOCIATED DISEASE: | |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | False |
| NIH RISK GROUP: | 3 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
IIIB |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| BuviA.12797.a.B1.GE39331 | Full length( BuviA.12797.a ) | 1 | 415 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| BuviA.12797.a.B1.PS02541 | Full length( BuviA.12797.a ) | 1 | 415 |
Structures
7RHE
DEPOSITED: 7/16/2021
DETERMINATION: XRay
CLONE: BuviA.12797.a.B1.GE39331
PROTEIN: BuviA.12797.a.B1.PS02541
Molecular models
| METHOD | RESULTS |
|---|---|
| comparative modelling | Robetta_81923 |
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| OrthoMCL: | OG5_213343 |
| PATRIC ID: | fig|269482.11.peg.5747 |
| RefSeq: | YP_001119351.1 |
| UniProt: | A4JE13 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MRSPHIGQGS NSANVRRYNE RLLLKTLRRA GSASKADLAR LANMTGTAVG SIIASLADAK LIEFAGRTEG QRGQPASLIR LDPRGAFGIG VHLDRMRIET ALVNFAGDVL GRRSHDTLLP PPAEVIEIVR HDIDAMQALL PAHERARLAG VGVAQPYNLG AWMRELGLAP DTFRAWEDVD FASDLGRTVS LPVFGENDGN AAAIAELFYG YGRQCDDFVY LFIGPAIGGG IAIDGDCLRG VTGNAGDIAM IPVLPSRLAS APPPRGPWDI LLARASLHAL VRHLRHHGET VESRADLEAC IARGLPAVTE WIDDCVDALA PALRAVLCVV DAPVVVLDAD TDAGLLDALT SRLRAALVAT APEARGTPTL VRGTFGADAG AIGAATLPMY FNFSPRAGIL RGARIESQEV NHVAI
NT Sequence
ATGAGAAGCC CGCACATCGG TCAGGGCAGC AACTCGGCCA ACGTGCGCCG TTACAACGAG CGCCTGTTGC TGAAGACGCT GCGCCGCGCG GGCAGCGCGT CGAAAGCCGA TCTCGCCCGA CTCGCGAACA TGACCGGCAC GGCCGTCGGC AGCATCATCG CGTCGCTCGC CGATGCGAAG CTGATCGAAT TCGCCGGGCG CACCGAAGGC CAGCGCGGCC AGCCGGCGTC GCTGATCCGT CTCGATCCGC GCGGCGCGTT CGGCATCGGC GTCCATCTCG ACCGCATGCG CATCGAGACG GCGCTCGTCA ATTTCGCCGG CGACGTACTC GGCCGCCGTT CGCACGACAC CCTGCTGCCG CCGCCCGCCG AAGTCATCGA GATCGTGCGC CACGACATCG ACGCGATGCA GGCTCTGCTG CCCGCGCACG AACGCGCGCG GCTCGCGGGC GTCGGCGTCG CCCAGCCGTA CAACCTCGGC GCGTGGATGC GCGAGCTCGG CCTGGCGCCC GACACGTTCC GCGCGTGGGA AGACGTCGAC TTCGCCAGCG ACCTCGGGCG CACGGTGTCG CTGCCCGTGT TCGGCGAGAA CGACGGCAAC GCGGCCGCCA TCGCCGAACT GTTCTACGGG TACGGCCGCC AGTGCGACGA CTTCGTCTAT CTGTTCATCG GCCCGGCGAT CGGCGGCGGC ATCGCGATCG ACGGCGACTG CCTGCGCGGC GTGACGGGCA ATGCCGGCGA CATCGCCATG ATCCCCGTGC TGCCGAGCCG GCTCGCCTCC GCGCCGCCGC CACGCGGGCC GTGGGACATC CTGCTCGCGC GCGCGTCGCT GCATGCGCTG GTGCGCCATC TGCGCCATCA CGGCGAGACG GTCGAAAGCC GCGCCGACCT CGAGGCGTGC ATCGCCCGCG GCCTGCCGGC GGTGACCGAA TGGATCGACG ACTGCGTCGA TGCGCTCGCA CCGGCACTGC GTGCGGTGCT GTGCGTCGTC GATGCGCCGG TGGTCGTGCT CGACGCCGAT ACCGACGCGG GCCTGCTCGA CGCGTTGACC AGCCGCCTGC GCGCTGCGCT CGTCGCCACC GCTCCGGAAG CGCGCGGCAC GCCGACGCTC GTGCGCGGCA CGTTCGGCGC CGACGCCGGC GCGATCGGCG CCGCGACGCT GCCGATGTAC TTCAACTTCT CCCCGCGGGC CGGCATCCTC AGGGGCGCCC GCATCGAATC GCAGGAGGTC AACCATGTCG CGATC
Details for BuviA.12797.a.B1.GE39331
| HARVESTED ON: | 7/10/2015 |
| SEQUENCED ON: | 7/6/2015 |
| EXPECTED MW: | 45kDa |
| OBSERVED MW: | 45kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Too many to count (100+) |
| TOTAL EXPRESSION LEVEL: | Moderate Expression |
| SOLUBLE EXPRESSION LEVEL | Moderate Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | |
| PERCENT IDENTITY: | data unavailable |
| PERCENT COVERAGE: | data unavailable |
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expected Protein Sequence
MAHHHHHHMR SPHIGQGSNS ANVRRYNERL LLKTLRRAGS ASKADLARLA NMTGTAVGSI IASLADAKLI EFAGRTEGQR GQPASLIRLD PRGAFGIGVH LDRMRIETAL VNFAGDVLGR RSHDTLLPPP AEVIEIVRHD IDAMQALLPA HERARLAGVG VAQPYNLGAW MRELGLAPDT FRAWEDVDFA SDLGRTVSLP VFGENDGNAA AIAELFYGYG RQCDDFVYLF IGPAIGGGIA IDGDCLRGVT GNAGDIAMIP VLPSRLASAP PPRGPWDILL ARASLHALVR HLRHHGETVE SRADLEACIA RGLPAVTEWI DDCVDALAPA LRAVLCVVDA PVVVLDADTD AGLLDALTSR LRAALVATAP EARGTPTLVR GTFGADAGAI GAATLPMYFN FSPRAGILRG ARIESQEVNH VAI
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgagaa gcccgcacat cggtcagggc agcaactcgg ccaacgtgcg ccgttacaac gagcgcctgt tgctgaagac gctgcgccgc gcgggcagcg cgtcgaaagc cgatctcgcc cgactcgcga acatgaccgg cacggccgtc ggcagcatca tcgcgtcgct cgccgatgcg aagctgatcg aattcgccgg gcgcaccgaa ggccagcgcg gccagccggc gtcgctgatc cgtctcgatc cgcgcggcgc gttcggcatc ggcgtccatc tcgaccgcat gcgcatcgag acggcgctcg tcaatttcgc cggcgacgta ctcggccgcc gttcgcacga caccctgctg ccgccgcccg ccgaagtcat cgagatcgtg cgccacgaca tcgacgcgat gcaggctctg ctgcccgcgc acgaacgcgc gcggctcgcg ggcgtcggcg tcgcccagcc gtacaacctc ggcgcgtgga tgcgcgagct cggcctggcg cccgacacgt tccgcgcgtg ggaagacgtc gacttcgcca gcgacctcgg gcgcacggtg tcgctgcccg tgttcggcga gaacgacggc aacgcggccg ccatcgccga actgttctac gggtacggcc gccagtgcga cgacttcgtc tatctgttca tcggcccggc gatcggcggc ggcatcgcga tcgacggcga ctgcctgcgc ggcgtgacgg gcaatgccgg cgacatcgcc atgatccccg tgctgccgag ccggctcgcc tccgcgccgc cgccacgcgg gccgtgggac atcctgctcg cgcgcgcgtc gctgcatgcg ctggtgcgcc atctgcgcca tcacggcgag acggtcgaaa gccgcgccga cctcgaggcg tgcatcgccc gcggcctgcc ggcggtgacc gaatggatcg acgactgcgt cgatgcgctc gcaccggcac tgcgtgcggt gctgtgcgtc gtcgatgcgc cggtggtcgt gctcgacgcc gataccgacg cgggcctgct cgacgcgttg accagccgcc tgcgcgctgc gctcgtcgcc accgctccgg aagcgcgcgg cacgccgacg ctcgtgcgcg gcacgttcgg cgccgacgcc ggcgcgatcg gcgccgcgac gctgccgatg tacttcaact tctccccgcg ggccggcatc ctcaggggcg cccgcatcga atcgcaggag gtcaaccatg tcgcgatctg agtaagatag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaattcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgcagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gctgcggtaa agctcatcag cgtggtcgtg aagcgattca cagatgtctg cctgttcatc cgcgtccagc tcgttgagtt tctccagaag cgttaatgtc tggcttctga taaagcgggc catgttaagg gcggtttttt cctgtttggt cactgatgcc tccgtgtaag ggggatttct gttcatgggg gtaatgatac cgatgaaacg agagaggatg ctcacgatac gggttactga tgatgaacat gcccggttac tggaacgttg tgagggtaaa caactggcgg tatggatgcg gcgggaccag agaaaaatca ctcagggtca atgccagcgc ttcgttaata cagatgtagg tgttccacag ggtagccagc agcatcctgc gatgcagatc cggaacataa tggtgcaggg cgctgacttc cgcgtttcca gactttacga aacacggaaa ccgaagacca ttcatgttgt tgctcaggtc gcagacgttt tgcagcagca gtcgcttcac gttcgctcgc gtatcggtga ttcattctgc taaccagtaa ggcaaccccg ccagcctagc cgggtcctca acgacaggag cacgatcatg cgcacccgtg gccaggaccc aacgctgccc gagatgcgcc gcgtgcggct gctggagatg gcggacgcga tggatatgtt ctgccaaggg ttggtttgcg cattcacagt tctccgcaag aattgattgg ctccaattct tggagtggtg aatccgttag cgaggtgccg ccggcttcca ttcaggtcga ggtggcccgg ctccatgcac cgcgacgcaa cgcggggagg cagacaaggt atagggcggc gcctacaatc catgccaacc cgttccatgt gctcgccgag gcggcataaa tcgccgtgac gatcagcggt ccagtgatcg aagttaggct ggtaagagcc gcgagcgatc cttgaagctg tccctgatgg tcgtcatcta cctgcctgga cagcatggcc tgcaacgcgg gcatcccgat gccgccggaa gcgagaagaa tcataatggg gaaggccatc cagcctcgcg tcgcgaacgc cagcaagacg tagcccagcg cgtcggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga aat
Details for BuviA.12797.a.B1.PS02541
| PURIFICATION DATe: | 11/18/2015 |
| CONCENTRATION: | 38.98mg/ml |
| OBSERVED MW: | 45kDa |
| EXPRESSION LEVEL: | Moderate Expression |
| PROTEIN PURIFICATION BUFFER: | SEC buffer |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 6 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | data unavailable |
| PERCENT COVERAGE: | data unavailable |
Protocol Notes
notes unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expressed Protein Sequence
MAHHHHHHMR SPHIGQGSNS ANVRRYNERL LLKTLRRAGS ASKADLARLA NMTGTAVGSI IASLADAKLI EFAGRTEGQR GQPASLIRLD PRGAFGIGVH LDRMRIETAL VNFAGDVLGR RSHDTLLPPP AEVIEIVRHD IDAMQALLPA HERARLAGVG VAQPYNLGAW MRELGLAPDT FRAWEDVDFA SDLGRTVSLP VFGENDGNAA AIAELFYGYG RQCDDFVYLF IGPAIGGGIA IDGDCLRGVT GNAGDIAMIP VLPSRLASAP PPRGPWDILL ARASLHALVR HLRHHGETVE SRADLEACIA RGLPAVTEWI DDCVDALAPA LRAVLCVVDA PVVVLDADTD AGLLDALTSR LRAALVATAP EARGTPTLVR GTFGADAGAI GAATLPMYFN FSPRAGILRG ARIESQEVNH VAI
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGAGAA GCCCGCACAT CGGTCAGGGC AGCAACTCGG CCAACGTGCG CCGTTACAAC GAGCGCCTGT TGCTGAAGAC GCTGCGCCGC GCGGGCAGCG CGTCGAAAGC CGATCTCGCC CGACTCGCGA ACATGACCGG CACGGCCGTC GGCAGCATCA TCGCGTCGCT CGCCGATGCG AAGCTGATCG AATTCGCCGG GCGCACCGAA GGCCAGCGCG GCCAGCCGGC GTCGCTGATC CGTCTCGATC CGCGCGGCGC GTTCGGCATC GGCGTCCATC TCGACCGCAT GCGCATCGAG ACGGCGCTCG TCAATTTCGC CGGCGACGTA CTCGGCCGCC GTTCGCACGA CACCCTGCTG CCGCCGCCCG CCGAAGTCAT CGAGATCGTG CGCCACGACA TCGACGCGAT GCAGGCTCTG CTGCCCGCGC ACGAACGCGC GCGGCTCGCG GGCGTCGGCG TCGCCCAGCC GTACAACCTC GGCGCGTGGA TGCGCGAGCT CGGCCTGGCG CCCGACACGT TCCGCGCGTG GGAAGACGTC GACTTCGCCA GCGACCTCGG GCGCACGGTG TCGCTGCCCG TGTTCGGCGA GAACGACGGC AACGCGGCCG CCATCGCCGA ACTGTTCTAC GGGTACGGCC GCCAGTGCGA CGACTTCGTC TATCTGTTCA TCGGCCCGGC GATCGGCGGC GGCATCGCGA TCGACGGCGA CTGCCTGCGC GGCGTGACGG GCAATGCCGG CGACATCGCC ATGATCCCCG TGCTGCCGAG CCGGCTCGCC TCCGCGCCGC CGCCACGCGG GCCGTGGGAC ATCCTGCTCG CGCGCGCGTC GCTGCATGCG CTGGTGCGCC ATCTGCGCCA TCACGGCGAG ACGGTCGAAA GCCGCGCCGA CCTCGAGGCG TGCATCGCCC GCGGCCTGCC GGCGGTGACC GAATGGATCG ACGACTGCGT CGATGCGCTC GCACCGGCAC TGCGTGCGGT GCTGTGCGTC GTCGATGCGC CGGTGGTCGT GCTCGACGCC GATACCGACG CGGGCCTGCT CGACGCGTTG ACCAGCCGCC TGCGCGCTGC GCTCGTCGCC ACCGCTCCGG AAGCGCGCGG CACGCCGACG CTCGTGCGCG GCACGTTCGG CGCCGACGCC GGCGCGATCG GCGCCGCGAC GCTGCCGATG TACTTCAACT TCTCCCCGCG GGCCGGCATC CTCAGGGGCG CCCGCATCGA ATCGCAGGAG GTCAACCATG TCGCGATCTG AGTAAGATAG GATCCGGCTG CTAACAAAGC CCGAAAGGAA GCTGAGTTGG CTGCTGCCAC CGCTGAGCAA TAACTAGCAT AACCCCTTGG GGCCTCTAAA CGGGTCTTGA GGGGTTTTTT GCTGAAAGGA GGAACTATAT CCGGATATCC ACAGGACGGG TGTGGTCGCC ATGATCGCGT AGTCGATAGT GGCTCCAAGT AGCGAAGCGA GCAGGACTGG GCGGCGGCCA AAGCGGTCGG ACAGTGCTCC GAGAACGGGT GCGCATAGAA ATTGCATCAA CGCATATAGC GCTAGCAGCA CGCCATAGTG ACTGGCGATG CTGTCGGAAT GGACGATATC CCGCAAGAGG CCCGGCAGTA CCGGCATAAC CAAGCCTATG CCTACAGCAT CCAGGGTGAC GGTGCCGAGG ATGACGATGA GCGCATTGTT AGATTTCATA CACGGTGCCT GACTGCGTTA GCAATTTAAC TGTGATAAAC TACCGCATTA AAGCTTATCG ATGATAAGCT GTCAAACATG AGAATTCTTG AAGACGAAAG GGCCTCGTGA TACGCCTATT TTTATAGGTT AATGTCATGA TAATAATGGT TTCTTAGACG TCAGGTGGCA CTTTTCGGGG AAATGTGCGC GGAACCCCTA TTTGTTTATT TTTCTAAATA CATTCAAATA TGTATCCGCT CATGAGACAA TAACCCTGAT AAATGCTTCA ATAATATTGA AAAAGGAAGA GTATGAGTAT TCAACATTTC CGTGTCGCCC TTATTCCCTT TTTTGCGGCA TTTTGCCTTC CTGTTTTTGC TCACCCAGAA ACGCTGGTGA AAGTAAAAGA TGCTGAAGAT CAGTTGGGTG CACGAGTGGG TTACATCGAA CTGGATCTCA ACAGCGGTAA GATCCTTGAG AGTTTTCGCC CCGAAGAACG TTTTCCAATG ATGAGCACTT TTAAAGTTCT GCTATGTGGC GCGGTATTAT CCCGTGTTGA CGCCGGGCAA GAGCAACTCG GTCGCCGCAT ACACTATTCT CAGAATGACT TGGTTGAGTA CTCACCAGTC ACAGAAAAGC ATCTTACGGA TGGCATGACA GTAAGAGAAT TATGCAGTGC TGCCATAACC ATGAGTGATA ACACTGCGGC CAACTTACTT CTGACAACGA TCGGAGGACC GAAGGAGCTA ACCGCTTTTT TGCACAACAT GGGGGATCAT GTAACTCGCC TTGATCGTTG GGAACCGGAG CTGAATGAAG CCATACCAAA CGACGAGCGT GACACCACGA TGCCTGCAGC AATGGCAACA ACGTTGCGCA AACTATTAAC TGGCGAACTA CTTACTCTAG CTTCCCGGCA ACAATTAATA GACTGGATGG AGGCGGATAA AGTTGCAGGA CCACTTCTGC GCTCGGCCCT TCCGGCTGGC TGGTTTATTG CTGATAAATC TGGAGCCGGT GAGCGTGGGT CTCGCGGTAT CATTGCAGCA CTGGGGCCAG ATGGTAAGCC CTCCCGTATC GTAGTTATCT ACACGACGGG GAGTCAGGCA ACTATGGATG AACGAAATAG ACAGATCGCT GAGATAGGTG CCTCACTGAT TAAGCATTGG TAACTGTCAG ACCAAGTTTA CTCATATATA CTTTAGATTG ATTTAAAACT TCATTTTTAA TTTAAAAGGA TCTAGGTGAA GATCCTTTTT GATAATCTCA TGACCAAAAT CCCTTAACGT GAGTTTTCGT TCCACTGAGC GTCAGACCCC GTAGAAAAGA TCAAAGGATC TTCTTGAGAT CCTTTTTTTC TGCGCGTAAT CTGCTGCTTG CAAACAAAAA AACCACCGCT ACCAGCGGTG GTTTGTTTGC CGGATCAAGA GCTACCAACT CTTTTTCCGA AGGTAACTGG CTTCAGCAGA GCGCAGATAC CAAATACTGT CCTTCTAGTG TAGCCGTAGT TAGGCCACCA CTTCAAGAAC TCTGTAGCAC CGCCTACATA CCTCGCTCTG CTAATCCTGT TACCAGTGGC TGCTGCCAGT GGCGATAAGT CGTGTCTTAC CGGGTTGGAC TCAAGACGAT AGTTACCGGA TAAGGCGCAG CGGTCGGGCT GAACGGGGGG TTCGTGCACA CAGCCCAGCT TGGAGCGAAC GACCTACACC GAACTGAGAT ACCTACAGCG TGAGCTATGA GAAAGCGCCA CGCTTCCCGA AGGGAGAAAG GCGGACAGGT ATCCGGTAAG CGGCAGGGTC GGAACAGGAG AGCGCACGAG GGAGCTTCCA GGGGGAAACG CCTGGTATCT TTATAGTCCT GTCGGGTTTC GCCACCTCTG ACTTGAGCGT CGATTTTTGT GATGCTCGTC AGGGGGGCGG AGCCTATGGA AAAACGCCAG CAACGCGGCC TTTTTACGGT TCCTGGCCTT TTGCTGGCCT TTTGCTCACA TGTTCTTTCC TGCGTTATCC CCTGATTCTG TGGATAACCG TATTACCGCC TTTGAGTGAG CTGATACCGC TCGCCGCAGC CGAACGACCG AGCGCAGCGA GTCAGTGAGC GAGGAAGCGG AAGAGCGCCT GATGCGGTAT TTTCTCCTTA CGCATCTGTG CGGTATTTCA CACCGCATAT ATGGTGCACT CTCAGTACAA TCTGCTCTGA TGCCGCATAG TTAAGCCAGT ATACACTCCG CTATCGCTAC GTGACTGGGT CATGGCTGCG CCCCGACACC CGCCAACACC CGCTGACGCG CCCTGACGGG CTTGTCTGCT CCCGGCATCC GCTTACAGAC AAGCTGTGAC CGTCTCCGGG AGCTGCATGT GTCAGAGGTT TTCACCGTCA TCACCGAAAC GCGCGAGGCA GCTGCGGTAA AGCTCATCAG CGTGGTCGTG AAGCGATTCA CAGATGTCTG CCTGTTCATC CGCGTCCAGC TCGTTGAGTT TCTCCAGAAG CGTTAATGTC TGGCTTCTGA TAAAGCGGGC CATGTTAAGG GCGGTTTTTT CCTGTTTGGT CACTGATGCC TCCGTGTAAG GGGGATTTCT GTTCATGGGG GTAATGATAC CGATGAAACG AGAGAGGATG CTCACGATAC GGGTTACTGA TGATGAACAT GCCCGGTTAC TGGAACGTTG TGAGGGTAAA CAACTGGCGG TATGGATGCG GCGGGACCAG AGAAAAATCA CTCAGGGTCA ATGCCAGCGC TTCGTTAATA CAGATGTAGG TGTTCCACAG GGTAGCCAGC AGCATCCTGC GATGCAGATC CGGAACATAA TGGTGCAGGG CGCTGACTTC CGCGTTTCCA GACTTTACGA AACACGGAAA CCGAAGACCA TTCATGTTGT TGCTCAGGTC GCAGACGTTT TGCAGCAGCA GTCGCTTCAC GTTCGCTCGC GTATCGGTGA TTCATTCTGC TAACCAGTAA GGCAACCCCG CCAGCCTAGC CGGGTCCTCA ACGACAGGAG CACGATCATG CGCACCCGTG GCCAGGACCC AACGCTGCCC GAGATGCGCC GCGTGCGGCT GCTGGAGATG GCGGACGCGA TGGATATGTT CTGCCAAGGG TTGGTTTGCG CATTCACAGT TCTCCGCAAG AATTGATTGG CTCCAATTCT TGGAGTGGTG AATCCGTTAG CGAGGTGCCG CCGGCTTCCA TTCAGGTCGA GGTGGCCCGG CTCCATGCAC CGCGACGCAA CGCGGGGAGG CAGACAAGGT ATAGGGCGGC GCCTACAATC CATGCCAACC CGTTCCATGT GCTCGCCGAG GCGGCATAAA TCGCCGTGAC GATCAGCGGT CCAGTGATCG AAGTTAGGCT GGTAAGAGCC GCGAGCGATC CTTGAAGCTG TCCCTGATGG TCGTCATCTA CCTGCCTGGA CAGCATGGCC TGCAACGCGG GCATCCCGAT GCCGCCGGAA GCGAGAAGAA TCATAATGGG GAAGGCCATC CAGCCTCGCG TCGCGAACGC CAGCAAGACG TAGCCCAGCG CGTCGGCCGC CATGCCGGCG ATAATGGCCT GCTTCTCGCC GAAACGTTTG GTGGCGGGAC CAGTGACGAA GGCTTGAGCG AGGGCGTGCA AGATTCCGAA TACCGCAAGC GACAGGCCGA TCATCGTCGC GCTCCAGCGA AAGCGGTCCT CGCCGAAAAT GACCCAGAGC GCTGCCGGCA CCTGTCCTAC GAGTTGCATG ATAAAGAAGA CAGTCATAAG TGCGGCGACG ATAGTCATGC CCCGCGCCCA CCGGAAGGAG CTGACTGGGT TGAAGGCTCT CAAGGGCATC GGTCGACGCT CTCCCTTATG CGACTCCTGC ATTAGGAAGC AGCCCAGTAG TAGGTTGAGG CCGTTGAGCA CCGCCGCCGC AAGGAATGGT GCATGCAAGG AGATGGCGCC CAACAGTCCC CCGGCCACGG GGCCTGCCAC CATACCCACG CCGAAACAAG CGCTCATGAG CCCGAAGTGG CGAGCCCGAT CTTCCCCATC GGTGATGTCG GCGATATAGG CGCCAGCAAC CGCACCTGTG GCGCCGGTGA TGCCGGCCAC GATGCGTCCG GCGTAGAGGA TCGAGATCTC GATCCCGCGA AAT