BuviA.12746.a

Beta-lactamase domain protein

CENTER ID: BuviA.12746.a
ORGANISM: Burkholderia vietnamiensis G4
ASSOCIATED DISEASE:
CURRENT STATUS: diffraction
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BuviA.12746.a.B1.GE39330 Full length( BuviA.12746.a ) 1 319
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|269482.11.peg.3583
RefSeq: YP_001117277.1
UniProt: A4JNF9

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MAKAFASQAD LEEKKITWTQ LSENAYAYTA EGDPNSGVIV GDDSVLIVDT TATPAMAQDL IAKIRSVTDK PIKHVVLSHY HAVRVLGASA YFAEGAQHVI ASRGTYEMIV ERGEADMKSE IERFPRLFAG VETVPGLTWP TLVFEREITL FLGKLEVKIM HVGSGHTKGD TIVWLPSQKV LFSGDLVEYD AACYCGDAQL EQWPATLEAL RALGADKLVP GRGPALLNPA DVNKGLDYTK DFVTTLLEQG RKAVERKLDL KASMALTREA MDPKFGHVFI YEHCLPFDVS RAFDEASGIT HPRIWTAQRD KEMWAALQD
NT Sequence
ATGGCCAAAG CATTCGCCTC CCAAGCCGAT CTCGAAGAGA AGAAGATCAC CTGGACGCAG CTGTCCGAGA ACGCGTACGC ATACACCGCC GAAGGCGACC CGAACTCGGG CGTGATCGTC GGCGACGACA GCGTGCTGAT CGTCGACACG ACCGCGACGC CCGCGATGGC GCAAGACCTG ATCGCGAAGA TCCGCAGCGT GACCGACAAG CCGATCAAGC ACGTCGTGCT GTCGCATTAC CACGCGGTGC GCGTGCTCGG CGCGTCCGCG TATTTCGCCG AAGGCGCGCA GCACGTGATC GCGAGCCGCG GCACCTACGA GATGATCGTC GAGCGCGGCG AAGCGGACAT GAAGTCGGAG ATCGAGCGCT TCCCGCGGCT GTTCGCGGGC GTCGAGACGG TGCCCGGCCT GACCTGGCCG ACACTCGTGT TCGAGCGCGA GATCACGCTG TTCCTCGGCA AGCTCGAAGT GAAGATCATG CACGTCGGCT CGGGCCATAC GAAGGGCGAC ACGATCGTCT GGCTGCCGTC GCAGAAGGTG CTGTTCTCCG GCGATCTGGT CGAATACGAC GCCGCGTGCT ATTGCGGCGA CGCGCAGCTC GAACAGTGGC CGGCCACGCT CGAGGCGTTG CGCGCGCTCG GCGCCGACAA GCTCGTGCCG GGCCGCGGCC CCGCGCTGCT GAACCCGGCC GACGTGAACA AGGGCCTCGA CTACACGAAG GACTTCGTGA CGACGCTGCT CGAGCAGGGC CGCAAGGCCG TCGAGCGCAA GCTCGACCTG AAGGCGTCGA TGGCGCTCAC GCGCGAAGCG ATGGACCCGA AGTTCGGCCA CGTGTTCATC TACGAGCACT GCCTGCCGTT CGACGTGTCG CGCGCGTTCG ACGAGGCGAG CGGCATCACG CATCCGCGCA TCTGGACCGC GCAGCGCGAC AAGGAAATGT GGGCCGCGCT GCAGGAC
Details for BuviA.12746.a.B1.GE39330
HARVESTED ON: 7/10/2015
SEQUENCED ON: 7/6/2015
EXPECTED MW: 36kDa
OBSERVED MW: 36kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY: 99
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMA KAFASQADLE EKKITWTQLS ENAYAYTAEG DPNSGVIVGD DSVLIVDTTA TPAMAQDLIA KIRSVTDKPI KHVVLSHYHA VRVLGASAYF AEGAQHVIAS RGTYEMIVER GEADMKSEIE RFPRLFAGVE TVPGLTWPTL VFEREITLFL GKLEVKIMHV GSGHTKGDTI VWLPSQKVLF SGDLVEYDAA CYCGDAQLEQ WPATLEALRA LGADKLVPGR GPALLNPADV NKGLDYTKDF VTTLLEQGRK AVERKLDLKA SMALTREAMD PKFGHVFIYE HCLPFDVSRA FDEASGITHP RIWTAQRDXX XWAALQD
Validated NT Sequence
ttactcactt agtcctgcag cgcggcccac annnnnntgt cgcgctgcgc ggtccagatg cgcggatgcg tgatgccgct cgcctcgtcg aacgcgcgcg acacgtcgaa cggcaggcag tgctcgtaga tgaacacgtg gccgaacttc gggtccatcg cttcgcgcgt gagcgccatc gacgccttca ggtcgagctt gcgctcgacg gccttgcggc cctgctcgag cagcgtcgtc acgaagtcct tcgtgtagtc gaggcccttg ttcacgtcgg ccgggttcag cagcgcgggg ccgcggcccg gcacgagctt gtcggcgccg agcgcgcgca acgcctcgag cgtggccggc cactgttcga gctgcgcgtc gccgcaatag cacgcggcgt cgtattcgac cagatcgccg gagaacagca ccttctgcga cggcagccag acgatcgtgt cgcccttcgt atggcccgag ccgacgtgca tgatcttcac ttcgagcttg ccgaggaaca gcgtgatctc gcgctcgaac acgagtgtcg gccaggtcag gccgggcacc gtctcgacgc ccgcgaacag ccgcgggaag cgctcgatct ccgacttcat gtccgcttcg ccgcgctcga cgatcatctc gtaggtgccg cggctcgcga tcacgtgctg cgcgccttcg gcgaaatacg cggacgcgcc gagcacgcgc accgcgtggt aatgcgacag cacgacgtgc ttgatcggct tgtcggtcac gctgcggatc ttcgcgatca ggtcttgcgc catcgcgggc gtcgcggtcg tgtcgacgat cagcacgctg tcgtcgccga cgatcacgcc cgagttcggg tcgccttcgg cggtgtatgc gtacgcgttc tcggacagct gcgtccaggt gatcttcttc tcttcgagat cggcttggga ggcgaatgct ttggccatat ggtggtggtg gtggtgagcc at
Expected Protein Sequence
MAHHHHHHMA KAFASQADLE EKKITWTQLS ENAYAYTAEG DPNSGVIVGD DSVLIVDTTA TPAMAQDLIA KIRSVTDKPI KHVVLSHYHA VRVLGASAYF AEGAQHVIAS RGTYEMIVER GEADMKSEIE RFPRLFAGVE TVPGLTWPTL VFEREITLFL GKLEVKIMHV GSGHTKGDTI VWLPSQKVLF SGDLVEYDAA CYCGDAQLEQ WPATLEALRA LGADKLVPGR GPALLNPADV NKGLDYTKDF VTTLLEQGRK AVERKLDLKA SMALTREAMD PKFGHVFIYE HCLPFDVSRA FDEASGITHP RIWTAQRDKE MWAALQD
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggcca aagcattcgc ctcccaagcc gatctcgaag agaagaagat cacctggacg cagctgtccg agaacgcgta cgcatacacc gccgaaggcg acccgaactc gggcgtgatc gtcggcgacg acagcgtgct gatcgtcgac acgaccgcga cgcccgcgat ggcgcaagac ctgatcgcga agatccgcag cgtgaccgac aagccgatca agcacgtcgt gctgtcgcat taccacgcgg tgcgcgtgct cggcgcgtcc gcgtatttcg ccgaaggcgc gcagcacgtg atcgcgagcc gcggcaccta cgagatgatc gtcgagcgcg gcgaagcgga catgaagtcg gagatcgagc gcttcccgcg gctgttcgcg ggcgtcgaga cggtgcccgg cctgacctgg ccgacactcg tgttcgagcg cgagatcacg ctgttcctcg gcaagctcga agtgaagatc atgcacgtcg gctcgggcca tacgaagggc gacacgatcg tctggctgcc gtcgcagaag gtgctgttct ccggcgatct ggtcgaatac gacgccgcgt gctattgcgg cgacgcgcag ctcgaacagt ggccggccac gctcgaggcg ttgcgcgcgc tcggcgccga caagctcgtg ccgggccgcg gccccgcgct gctgaacccg gccgacgtga acaagggcct cgactacacg aaggacttcg tgacgacgct gctcgagcag ggccgcaagg ccgtcgagcg caagctcgac ctgaaggcgt cgatggcgct cacgcgcgaa gcgatggacc cgaagttcgg ccacgtgttc atctacgagc actgcctgcc gttcgacgtg tcgcgcgcgt tcgacgaggc gagcggcatc acgcatccgc gcatctggac cgcgcagcgc gacaaggaaa tgtgggccgc gctgcaggac tgagtaagat aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaattct tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgca gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg cagctgcggt aaagctcatc agcgtggtcg tgaagcgatt cacagatgtc tgcctgttca tccgcgtcca gctcgttgag tttctccaga agcgttaatg tctggcttct gataaagcgg gccatgttaa gggcggtttt ttcctgtttg gtcactgatg cctccgtgta agggggattt ctgttcatgg gggtaatgat accgatgaaa cgagagagga tgctcacgat acgggttact gatgatgaac atgcccggtt actggaacgt tgtgagggta aacaactggc ggtatggatg cggcgggacc agagaaaaat cactcagggt caatgccagc gcttcgttaa tacagatgta ggtgttccac agggtagcca gcagcatcct gcgatgcaga tccggaacat aatggtgcag ggcgctgact tccgcgtttc cagactttac gaaacacgga aaccgaagac cattcatgtt gttgctcagg tcgcagacgt tttgcagcag cagtcgcttc acgttcgctc gcgtatcggt gattcattct gctaaccagt aaggcaaccc cgccagccta gccgggtcct caacgacagg agcacgatca tgcgcacccg tggccaggac ccaacgctgc ccgagatgcg ccgcgtgcgg ctgctggaga tggcggacgc gatggatatg ttctgccaag ggttggtttg cgcattcaca gttctccgca agaattgatt ggctccaatt cttggagtgg tgaatccgtt agcgaggtgc cgccggcttc cattcaggtc gaggtggccc ggctccatgc accgcgacgc aacgcgggga ggcagacaag gtatagggcg gcgcctacaa tccatgccaa cccgttccat gtgctcgccg aggcggcata aatcgccgtg acgatcagcg gtccagtgat cgaagttagg ctggtaagag ccgcgagcga tccttgaagc tgtccctgat ggtcgtcatc tacctgcctg gacagcatgg cctgcaacgc gggcatcccg atgccgccgg aagcgagaag aatcataatg gggaaggcca tccagcctcg cgtcgcgaac gccagcaaga cgtagcccag cgcgtcggcc gccatgccgg cgataatggc ctgcttctcg ccgaaacgtt tggtggcggg accagtgacg aaggcttgag cgagggcgtg caagattccg aataccgcaa gcgacaggcc gatcatcgtc gcgctccagc gaaagcggtc ctcgccgaaa atgacccaga gcgctgccgg cacctgtcct acgagttgca tgataaagaa gacagtcata agtgcggcga cgatagtcat gccccgcgcc caccggaagg agctgactgg gttgaaggct ctcaagggca tcggtcgacg ctctccctta tgcgactcct gcattaggaa gcagcccagt agtaggttga ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg cccaacagtc ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg agcccgaagt ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca accgcacctg tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gatcgagatc tcgatcccgc gaaat