BuviA.12707.a

Squalene/phytoene synthase

CENTER ID: BuviA.12707.a
ORGANISM: Burkholderia vietnamiensis G4
ASSOCIATED DISEASE:
CURRENT STATUS: purified
COMMUNITY REQUEST: False
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BuviA.12707.a.B1.GE39329 Full length( BuviA.12707.a ) 1 286
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|269482.11.peg.2521
RefSeq: YP_001116254.1
UniProt: A4JKI6

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MELVLAVSNL VVDEQETDAA AVTSGSSFYL AMRILPAAQR DAMFQVYAFC RAVDDIADSD LPRAERNAGL DRWRADIDAC FAGQPPRHLV ALEREIRAFD LRREDFHAMI DGMAMDAAED ICAPDEPTLD LFCDRVASAA GRLSVRIFGI PEAEGIELSH HLGRALQLTN ILRDIDDDAA INRCYLPREL LAREGIAIAD PATIVRDPAL PRVCATLVER ALAHFRAADA VMDTCTRAQV KAPRIMSGAY RCILEAAIAR GFAYPRAPLR KPKARMLMIA ARYALF
NT Sequence
GTGGAGCTCG TCTTGGCCGT TTCCAATCTC GTCGTGGACG AACAAGAAAC CGACGCCGCT GCCGTCACAT CGGGCAGCTC CTTCTATCTG GCGATGCGCA TCCTGCCGGC CGCGCAGCGC GACGCGATGT TCCAGGTCTA CGCGTTCTGT CGCGCCGTCG ACGACATCGC CGACAGCGAC CTGCCGCGCG CCGAGCGCAA CGCGGGCCTC GACCGCTGGC GCGCGGACAT CGACGCGTGC TTCGCCGGCC AGCCGCCGCG CCATCTGGTT GCGCTCGAGC GCGAGATCCG CGCGTTCGAC CTGCGCCGCG AAGACTTTCA CGCCATGATC GACGGCATGG CGATGGACGC CGCCGAGGAT ATCTGCGCGC CCGACGAGCC GACGCTCGAC CTGTTCTGCG ATCGCGTCGC CAGCGCGGCC GGCCGCTTGT CGGTGAGGAT TTTCGGGATC CCCGAAGCGG AAGGCATCGA GCTGTCGCAC CATCTCGGCC GCGCGTTGCA GCTGACGAAC ATCCTGCGCG ACATCGACGA CGACGCGGCG ATCAACCGCT GCTACCTGCC GCGCGAGCTG CTCGCGCGCG AGGGCATCGC GATCGCCGAT CCCGCGACGA TCGTGCGCGA CCCGGCGCTG CCGCGCGTGT GCGCGACGCT CGTCGAACGC GCGCTCGCGC ACTTCCGCGC CGCCGATGCG GTGATGGACA CCTGCACGCG CGCGCAGGTG AAGGCGCCGC GCATCATGTC GGGCGCGTAT CGCTGCATTC TGGAAGCCGC GATCGCGCGC GGTTTCGCGT ATCCGCGCGC GCCGCTGCGC AAGCCGAAGG CGCGCATGCT GATGATCGCC GCGCGCTACG CGCTGTTC
Details for BuviA.12707.a.B1.GE39329
HARVESTED ON: 7/10/2015
SEQUENCED ON: 7/6/2015
EXPECTED MW: 33kDa
OBSERVED MW: 33kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY: 99
PERCENT COVERAGE: 99
Validated AA Sequence
MAHHHHHHMV ELVLAVSNLV VDEQETDAAA VTSGSSFYLA MRILPAAQRD AMFQVYAFCR AVDDIADSDL PRAERNAGLD RWRADIDACF AGQPPRHLVA LEREIRAFDL RREDFHAMID GMAMDAAEDI CAPDEPTLDL FCDRVASAAG RLSVRIFGIP EAEGIELSHH LGRALQLTNI LRDIDDDAAI NRCYLPRELL AREGIAIADP ATIVRDPALP RVCATLVERA LAHFRAADAV MDTCTRAQVK APRIMSGAYR CILEAAIARG FAYPRAPLRK PKARMLXXAA RY
Validated NT Sequence
cgcgtagcgc gcggcgnnnn nnagcatgcg cgccttcggc ttgcgcagcg gcgcgcgcgg atacgcgaaa ccgcgcgcga tcgcggcttc cagaatgcag cgatacgcgc ccgacatgat gcgcggcgcc ttcacctgcg cgcgcgtgca ggtgtccatc accgcatcgg cggcgcggaa gtgcgcgagc gcgcgttcga cgagcgtcgc gcacacgcgc ggcagcgccg ggtcgcgcac gatcgtcgcg ggatcggcga tcgcgatgcc ctcgcgcgcg agcagctcgc gcggcaggta gcagcggttg atcgccgcgt cgtcgtcgat gtcgcgcagg atgttcgtca gctgcaacgc gcggccgaga tggtgcgaca gctcgatgcc ttccgcttcg gggatcccga aaatcctcac cgacaagcgg ccggccgcgc tggcgacgcg atcgcagaac aggtcgagcg tcggctcgtc gggcgcgcag atatcctcgg cggcgtccat cgccatgccg tcgatcatgg cgtgaaagtc ttcgcggcgc aggtcgaacg cgcggatctc gcgctcgagc gcaaccagat ggcgcggcgg ctggccggcg aagcacgcgt cgatgtccgc gcgccagcgg tcgaggcccg cgttgcgctc ggcgcgcggc aggtcgctgt cggcgatgtc gtcgacggcg cgacagaacg cgtagacctg gaacatcgcg tcgcgctgcg cggccggcag gatgcgcatc gccagataga aggagctgcc cgatgtgacg gcagcggcgt cggtttcttg ttcgtccacg acgagattgg aaacggccaa gacgagctcc accatatggt ggtggtggtg gtgagccat
Expected Protein Sequence
MAHHHHHHME LVLAVSNLVV DEQETDAAAV TSGSSFYLAM RILPAAQRDA MFQVYAFCRA VDDIADSDLP RAERNAGLDR WRADIDACFA GQPPRHLVAL EREIRAFDLR REDFHAMIDG MAMDAAEDIC APDEPTLDLF CDRVASAAGR LSVRIFGIPE AEGIELSHHL GRALQLTNIL RDIDDDAAIN RCYLPRELLA REGIAIADPA TIVRDPALPR VCATLVERAL AHFRAADAVM DTCTRAQVKA PRIMSGAYRC ILEAAIARGF AYPRAPLRKP KARMLMIAAR YALF
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgtggagc tcgtcttggc cgtttccaat ctcgtcgtgg acgaacaaga aaccgacgcc gctgccgtca catcgggcag ctccttctat ctggcgatgc gcatcctgcc ggccgcgcag cgcgacgcga tgttccaggt ctacgcgttc tgtcgcgccg tcgacgacat cgccgacagc gacctgccgc gcgccgagcg caacgcgggc ctcgaccgct ggcgcgcgga catcgacgcg tgcttcgccg gccagccgcc gcgccatctg gttgcgctcg agcgcgagat ccgcgcgttc gacctgcgcc gcgaagactt tcacgccatg atcgacggca tggcgatgga cgccgccgag gatatctgcg cgcccgacga gccgacgctc gacctgttct gcgatcgcgt cgccagcgcg gccggccgct tgtcggtgag gattttcggg atccccgaag cggaaggcat cgagctgtcg caccatctcg gccgcgcgtt gcagctgacg aacatcctgc gcgacatcga cgacgacgcg gcgatcaacc gctgctacct gccgcgcgag ctgctcgcgc gcgagggcat cgcgatcgcc gatcccgcga cgatcgtgcg cgacccggcg ctgccgcgcg tgtgcgcgac gctcgtcgaa cgcgcgctcg cgcacttccg cgccgccgat gcggtgatgg acacctgcac gcgcgcgcag gtgaaggcgc cgcgcatcat gtcgggcgcg tatcgctgca ttctggaagc cgcgatcgcg cgcggtttcg cgtatccgcg cgcgccgctg cgcaagccga aggcgcgcat gctgatgatc gccgcgcgct acgcgctgtt ctgagtaaga taggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaattc ttgaagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgc agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggccagga cccaacgctg cccgagatgc gccgcgtgcg gctgctggag atggcggacg cgatggatat gttctgccaa gggttggttt gcgcattcac agttctccgc aagaattgat tggctccaat tcttggagtg gtgaatccgt tagcgaggtg ccgccggctt ccattcaggt cgaggtggcc cggctccatg caccgcgacg caacgcgggg aggcagacaa ggtatagggc ggcgcctaca atccatgcca acccgttcca tgtgctcgcc gaggcggcat aaatcgccgt gacgatcagc ggtccagtga tcgaagttag gctggtaaga gccgcgagcg atccttgaag ctgtccctga tggtcgtcat ctacctgcct ggacagcatg gcctgcaacg cgggcatccc gatgccgccg gaagcgagaa gaatcataat ggggaaggcc atccagcctc gcgtcgcgaa cgccagcaag acgtagccca gcgcgtcggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaat