BuviA.12245.d

Amidase, hydantoinase/carbamoylase family

CENTER ID: BuviA.12245.d
ORGANISM: Burkholderia vietnamiensis G4
ASSOCIATED DISEASE:
CURRENT STATUS: crystal structure
COMMUNITY REQUEST: False
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BuviA.12245.d.B1.GE39328 Full length( BuviA.12245.d ) 1 445
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.
External Resources
RESOURCE REFERENCE ID
OrthoMCL: OG5_130721
PATRIC ID: fig|269482.11.peg.669
RefSeq: YP_001110387.1
UniProt: A4JTI1
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MNTSHDPVPP LQLDAALMID QLRELGEIGA DVEAGGRTRI ALSDEERAGR DLVVKWMREL DLDVQIDRIG NVFGTLRSAF DDGSQRPLMM GSHIDTVTNA GALDGCYGVL AALAVVGAFR RAGVVPQRSI TVAAFTNEEG IRYQPDMMGS LVYAGGLPVD AALNTIGVDG TRLGDELARI GYAGELEPGA IIPHEYLELH IEQGPILEAE NFRIGVVENL QGISWQQITV QGNANHAGTT PMHLRHDAGW VAATVATFLR ELAAATGTTL ATIGMLRMEP NVINVIPRKA VFSVDLRDPD EQRLQDAERR LAEFLMQVAE KEGVAITTER LARFEPVVFD SGLVDAVEAS AKRFGFTHRR MTSGAGHDAQ MIARIAPAAM IFVPSRGGIS HNPREHTDDD QLIDGANLLL DVVLRRIAEI RDDPSFPTTV CVSQIKGKDG THAPI
NT Sequence
ATGAATACTT CTCACGACCC GGTACCACCG CTTCAACTGG ACGCGGCGCT CATGATCGAC CAGTTGCGCG AACTCGGGGA AATCGGCGCC GACGTCGAAG CGGGAGGCCG CACACGTATC GCACTGTCTG ACGAGGAGCG AGCGGGGCGC GATCTCGTCG TCAAGTGGAT GCGTGAACTG GATCTCGATG TGCAAATCGA TCGAATCGGC AACGTGTTCG GTACGCTGCG TTCGGCGTTC GACGACGGCA GTCAGCGTCC GTTGATGATG GGCTCTCACA TCGATACGGT AACGAATGCC GGCGCGCTGG ATGGCTGCTA TGGTGTGCTG GCGGCACTTG CCGTGGTAGG TGCGTTCCGG CGGGCGGGTG TCGTGCCGCA GCGATCGATC ACGGTTGCCG CGTTCACGAA CGAGGAGGGG ATTCGCTACC AGCCAGACAT GATGGGATCG CTCGTCTATG CGGGTGGGTT ACCCGTCGAC GCGGCGCTGA ACACGATTGG TGTCGACGGC ACGCGTCTGG GCGATGAACT CGCCCGCATC GGGTATGCCG GCGAACTGGA GCCTGGTGCG ATCATCCCCC ACGAATATCT CGAACTCCAT ATCGAGCAGG GGCCGATACT CGAAGCGGAA AATTTCCGTA TTGGCGTGGT CGAGAATCTC CAGGGCATCT CGTGGCAGCA GATCACCGTG CAGGGAAATG CCAATCACGC GGGAACGACG CCCATGCATT TGCGTCACGA CGCTGGGTGG GTTGCTGCGA CAGTCGCAAC GTTCCTGCGG GAGCTTGCGG CAGCAACAGG CACGACGCTG GCAACCATCG GCATGCTGCG AATGGAGCCG AATGTTATCA ACGTTATTCC TCGCAAGGCC GTGTTCTCGG TCGATCTTCG CGATCCAGAT GAACAGCGCC TGCAGGACGC GGAACGGCGC CTCGCGGAAT TCTTGATGCA GGTTGCCGAG AAGGAAGGTG TCGCGATCAC GACAGAGCGC CTCGCGCGTT TCGAACCCGT AGTATTCGAT TCCGGGCTTG TTGACGCAGT TGAAGCGTCA GCCAAGCGGT TTGGCTTCAC GCATCGCCGG ATGACTTCTG GCGCCGGCCA CGATGCCCAG ATGATCGCGC GCATCGCTCC GGCAGCGATG ATATTCGTGC CGAGCCGTGG CGGAATCAGC CACAATCCGC GCGAACACAC AGACGACGAT CAACTCATCG ATGGGGCGAA CCTTCTACTG GATGTTGTGC TCCGGCGTAT TGCTGAGATC CGCGACGATC CTTCGTTCCC GACGACGGTC TGTGTTTCCC AGATCAAAGG CAAGGACGGC ACGCACGCCC CGATC
Details for BuviA.12245.d.B1.GE39328
HARVESTED ON: 7/10/2015
SEQUENCED ON: 7/6/2015
EXPECTED MW: 49kDa
OBSERVED MW: 46kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expected Protein Sequence
MAHHHHHHMN TSHDPVPPLQ LDAALMIDQL RELGEIGADV EAGGRTRIAL SDEERAGRDL VVKWMRELDL DVQIDRIGNV FGTLRSAFDD GSQRPLMMGS HIDTVTNAGA LDGCYGVLAA LAVVGAFRRA GVVPQRSITV AAFTNEEGIR YQPDMMGSLV YAGGLPVDAA LNTIGVDGTR LGDELARIGY AGELEPGAII PHEYLELHIE QGPILEAENF RIGVVENLQG ISWQQITVQG NANHAGTTPM HLRHDAGWVA ATVATFLREL AAATGTTLAT IGMLRMEPNV INVIPRKAVF SVDLRDPDEQ RLQDAERRLA EFLMQVAEKE GVAITTERLA RFEPVVFDSG LVDAVEASAK RFGFTHRRMT SGAGHDAQMI ARIAPAAMIF VPSRGGISHN PREHTDDDQL IDGANLLLDV VLRRIAEIRD DPSFPTTVCV SQIKGKDGTH API
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgaata cttctcacga cccggtacca ccgcttcaac tggacgcggc gctcatgatc gaccagttgc gcgaactcgg ggaaatcggc gccgacgtcg aagcgggagg ccgcacacgt atcgcactgt ctgacgagga gcgagcgggg cgcgatctcg tcgtcaagtg gatgcgtgaa ctggatctcg atgtgcaaat cgatcgaatc ggcaacgtgt tcggtacgct gcgttcggcg ttcgacgacg gcagtcagcg tccgttgatg atgggctctc acatcgatac ggtaacgaat gccggcgcgc tggatggctg ctatggtgtg ctggcggcac ttgccgtggt aggtgcgttc cggcgggcgg gtgtcgtgcc gcagcgatcg atcacggttg ccgcgttcac gaacgaggag gggattcgct accagccaga catgatggga tcgctcgtct atgcgggtgg gttacccgtc gacgcggcgc tgaacacgat tggtgtcgac ggcacgcgtc tgggcgatga actcgcccgc atcgggtatg ccggcgaact ggagcctggt gcgatcatcc cccacgaata tctcgaactc catatcgagc aggggccgat actcgaagcg gaaaatttcc gtattggcgt ggtcgagaat ctccagggca tctcgtggca gcagatcacc gtgcagggaa atgccaatca cgcgggaacg acgcccatgc atttgcgtca cgacgctggg tgggttgctg cgacagtcgc aacgttcctg cgggagcttg cggcagcaac aggcacgacg ctggcaacca tcggcatgct gcgaatggag ccgaatgtta tcaacgttat tcctcgcaag gccgtgttct cggtcgatct tcgcgatcca gatgaacagc gcctgcagga cgcggaacgg cgcctcgcgg aattcttgat gcaggttgcc gagaaggaag gtgtcgcgat cacgacagag cgcctcgcgc gtttcgaacc cgtagtattc gattccgggc ttgttgacgc agttgaagcg tcagccaagc ggtttggctt cacgcatcgc cggatgactt ctggcgccgg ccacgatgcc cagatgatcg cgcgcatcgc tccggcagcg atgatattcg tgccgagccg tggcggaatc agccacaatc cgcgcgaaca cacagacgac gatcaactca tcgatggggc gaaccttcta ctggatgttg tgctccggcg tattgctgag atccgcgacg atccttcgtt cccgacgacg gtctgtgttt cccagatcaa aggcaaggac ggcacgcacg ccccgatctg agtaagatag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaattcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgcagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gctgcggtaa agctcatcag cgtggtcgtg aagcgattca cagatgtctg cctgttcatc cgcgtccagc tcgttgagtt tctccagaag cgttaatgtc tggcttctga taaagcgggc catgttaagg gcggtttttt cctgtttggt cactgatgcc tccgtgtaag ggggatttct gttcatgggg gtaatgatac cgatgaaacg agagaggatg ctcacgatac gggttactga tgatgaacat gcccggttac tggaacgttg tgagggtaaa caactggcgg tatggatgcg gcgggaccag agaaaaatca ctcagggtca atgccagcgc ttcgttaata cagatgtagg tgttccacag ggtagccagc agcatcctgc gatgcagatc cggaacataa tggtgcaggg cgctgacttc cgcgtttcca gactttacga aacacggaaa ccgaagacca ttcatgttgt tgctcaggtc gcagacgttt tgcagcagca gtcgcttcac gttcgctcgc gtatcggtga ttcattctgc taaccagtaa ggcaaccccg ccagcctagc cgggtcctca acgacaggag cacgatcatg cgcacccgtg gccaggaccc aacgctgccc gagatgcgcc gcgtgcggct gctggagatg gcggacgcga tggatatgtt ctgccaaggg ttggtttgcg cattcacagt tctccgcaag aattgattgg ctccaattct tggagtggtg aatccgttag cgaggtgccg ccggcttcca ttcaggtcga ggtggcccgg ctccatgcac cgcgacgcaa cgcggggagg cagacaaggt atagggcggc gcctacaatc catgccaacc cgttccatgt gctcgccgag gcggcataaa tcgccgtgac gatcagcggt ccagtgatcg aagttaggct ggtaagagcc gcgagcgatc cttgaagctg tccctgatgg tcgtcatcta cctgcctgga cagcatggcc tgcaacgcgg gcatcccgat gccgccggaa gcgagaagaa tcataatggg gaaggccatc cagcctcgcg tcgcgaacgc cagcaagacg tagcccagcg cgtcggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga aat