SSGCID: Target Page

BuviA.12245.c

Amidase, hydantoinase/carbamoylase family

CENTER ID:	BuviA.12245.c
ORGANISM:	Burkholderia vietnamiensis G4
ASSOCIATED DISEASE:
CURRENT STATUS:	purified
COMMUNITY REQUEST:	False
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BuviA.12245.c.B1.GE39327	Full length( BuviA.12245.c )		1	426

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|269482.11.peg.1943
RefSeq:	YP_001115732.1
UniProt:	A4JT74

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MQVQDSTPGV AALGLRVDGA RLWDSLMRLA QIGATANGGV CRLALTERDR DARNLFVGWA KEIGCTVRID AIGNIFARRA GACDDLPPVM TGSHIDTQPT GGKFDGNYGV LAGLEVLRTL DDAGVRTRAP LEVAVWTNEE GSRFVPVMMG SGVFARAFTL EHALAQRDRD GVAVRDALAA TGYAGDVRDA HPVGAYFEAH IEQGPVLEAH ATTIGVVEGA LGQRWYDVTV HGMEAHAGPT PMALRRDALL VAADLVRAVN GIALAHPPHG RGTVGWIDVH PNSRNVIPGR VTLTVDLRAA DDATLAAMDA ALRAACADLA ATAGMPIDVE QVVYFAPQPF DAALVEQVRA GANALGLSSM NVISGAGHDA VYLARVAPAA MIFVPCKDGI SHNEIEDAAP ADLEAGCNVL LHAMLAAAGL AEGYAR

NT Sequence

ATGCAAGTTC AGGATTCGAC ACCGGGCGTT GCCGCGCTCG GGCTGCGCGT CGACGGCGCG CGGCTATGGG ACAGCCTGAT GCGGCTCGCG CAAATCGGCG CGACCGCCAA CGGCGGCGTG TGCCGCCTCG CGCTCACCGA GCGCGATCGA GACGCGCGCA ACCTGTTCGT CGGATGGGCG AAGGAGATCG GCTGCACGGT CCGCATCGAT GCGATCGGCA ACATCTTTGC GCGCCGCGCG GGTGCGTGCG ACGACCTGCC GCCCGTGATG ACGGGCAGCC ATATCGACAC GCAGCCGACC GGCGGCAAGT TCGACGGCAA CTACGGCGTG CTCGCGGGCC TCGAAGTGCT GCGCACGCTC GACGACGCGG GCGTGCGCAC GCGCGCGCCG CTCGAGGTCG CGGTGTGGAC CAACGAGGAA GGCTCGCGCT TCGTGCCGGT GATGATGGGG TCCGGCGTGT TCGCGCGTGC GTTCACGCTC GAGCATGCAC TCGCGCAACG CGATCGCGAC GGCGTCGCCG TGCGCGATGC GCTCGCAGCG ACCGGCTATG CGGGGGACGT GCGCGATGCG CATCCGGTCG GCGCGTACTT CGAAGCGCAT ATCGAACAGG GGCCGGTGCT CGAGGCGCAT GCGACGACGA TCGGCGTGGT GGAGGGCGCG CTCGGGCAGC GCTGGTACGA CGTCACCGTG CACGGGATGG AGGCGCACGC CGGCCCGACG CCGATGGCGC TGCGGCGCGA CGCGCTGCTG GTGGCGGCCG ATCTCGTGCG CGCGGTGAAC GGCATCGCGC TCGCCCACCC GCCGCACGGG CGCGGCACGG TCGGCTGGAT CGACGTGCAT CCGAATTCGC GCAACGTGAT TCCGGGCCGC GTGACGCTGA CGGTCGATCT GCGCGCGGCC GACGATGCGA CGCTCGCGGC GATGGATGCG GCTCTGCGTG CAGCGTGTGC CGATCTCGCG GCGACGGCCG GCATGCCGAT CGACGTCGAG CAGGTCGTGT ACTTCGCGCC TCAGCCGTTC GACGCGGCGC TCGTCGAACA GGTGCGCGCG GGCGCGAATG CGCTGGGCCT GTCGTCGATG AACGTGATCA GCGGGGCCGG CCACGATGCC GTGTATCTCG CGCGTGTCGC GCCCGCCGCG ATGATCTTCG TGCCGTGCAA GGACGGGATC AGCCACAACG AAATCGAGGA CGCGGCGCCT GCCGATCTCG AGGCCGGCTG CAACGTGCTG CTCCATGCGA TGCTCGCCGC AGCCGGCCTC GCGGAGGGGT ACGCGCGA

Details for BuviA.12245.c.B1.GE39327

HARVESTED ON:	7/10/2015
SEQUENCED ON:	7/6/2015
EXPECTED MW:	46kDa
OBSERVED MW:	46kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	High Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY:	100
PERCENT COVERAGE:	97

Validated AA Sequence

MAHHHHHHMQ VQDSTPGVAA LGLRVDGARL WDSLMRLAQI GATANGGVCR LALTERDRDA RNLFVGWAKE IGCTVRIDAI GNIFARRAGA CDDLPPVMTG SHIDTQPTGG KFDGNYGVLA GLEVLRTLDD AGVRTRAPLE VAVWTNEEGS RFVPVMMGSG VFARAFTLEH ALAQRDRDGV AVRDALAATG YAGDVRDAHP VGAYFEAHIE QGPVLEAHAT TIGVVEGALG QRWYDVTVHG MEAHAGPTPM ALRRDALLVA ADLVRAVNGI ALAHPPHGRG TVGWIDVHPN SRNVIPGRVT LTVDLRAADD ATLAAMDAAL RAACADLAAT AGMPIDVEQV VYFAPQPFDA ALVEQVRAGA NALGLSSMNV ISGAGHDAVY LARVAPAAMI FVPCKDGISH NEIEDAAPAD LEAGCNVLLH AMLA

Validated NT Sequence

tgcggcgagc atcgcatgga gcagcacgtt gcagccggcc tcgagatcgg caggcgccgc gtcctcgatt tcgttgtggc tgatcccgtc cttgcacggc acgaagatca tcgcggcggg cgcgacacgc gcgagataca cggcatcgtg gccggccccg ctgatcacgt tcatcgacga caggcccagc gcattcgcgc ccgcgcgcac ctgttcgacg agcgccgcgt cgaacggctg aggcgcgaag tacacgacct gctcgacgtc gatcggcatg ccggccgtcg ccgcgagatc ggcacacgct gcacgcagag ccgcatccat cgccgcgagc gtcgcatcgt cggccgcgcg cagatcgacc gtcagcgtca cgcggcccgg aatcacgttg cgcgaattcg gatgcacgtc gatccagccg accgtgccgc gcccgtgcgg cgggtgggcg agcgcgatgc cgttcaccgc gcgcacgaga tcggccgcca ccagcagcgc gtcgcgccgc agcgccatcg gcgtcgggcc ggcgtgcgcc tccatcccgt gcacggtgac gtcgtaccag cgctgcccga gcgcgccctc caccacgccg atcgtcgtcg catgcgcctc gagcaccggc ccctgttcga tatgcgcttc gaagtacgcg ccgaccggat gcgcatcgcg cacgtccccc gcatagccgg tcgctgcgag cgcatcgcgc acggcgacgc cgtcgcgatc gcgttgcgcg agtgcatgct cgagcgtgaa cgcacgcgcg aacacgccgg accccatcat caccggcacg aagcgcgagc cttcctcgtt ggtccacacc gcgacctcga gcggcgcgcg cgtgcgcacg cccgcgtcgt cgagcgtgcg cagcacttcg aggcccgcga gcacgccgta gttgccgtcg aacttgccgc cggtcggctg cgtgtcgata tggctgcccg tcatcacggg cggcaggtcg tcgcacgcac ccgcgcggcg cgcaaagatg ttgccgatcg catcgatgcg gaccgtgcag ccgatctcct tcgcccatcc gacgaacagg ttgcgcgcgt ctcgatcgcg ctcggtgagc gcgaggcggc acacgccgcc gttggcggtc gcgccgattt gcgcgagccg catcaggctg tcccatagcc gcgcgccgtc gacgcgcagc ccgagcgcgg caacgcccgg tgtcgaatcc tgaacttgca tatggtggtg gtggtggtga gccat

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgcaag ttcaggattc gacaccgggc gttgccgcgc tcgggctgcg cgtcgacggc gcgcggctat gggacagcct gatgcggctc gcgcaaatcg gcgcgaccgc caacggcggc gtgtgccgcc tcgcgctcac cgagcgcgat cgagacgcgc gcaacctgtt cgtcggatgg gcgaaggaga tcggctgcac ggtccgcatc gatgcgatcg gcaacatctt tgcgcgccgc gcgggtgcgt gcgacgacct gccgcccgtg atgacgggca gccatatcga cacgcagccg accggcggca agttcgacgg caactacggc gtgctcgcgg gcctcgaagt gctgcgcacg ctcgacgacg cgggcgtgcg cacgcgcgcg ccgctcgagg tcgcggtgtg gaccaacgag gaaggctcgc gcttcgtgcc ggtgatgatg gggtccggcg tgttcgcgcg tgcgttcacg ctcgagcatg cactcgcgca acgcgatcgc gacggcgtcg ccgtgcgcga tgcgctcgca gcgaccggct atgcggggga cgtgcgcgat gcgcatccgg tcggcgcgta cttcgaagcg catatcgaac aggggccggt gctcgaggcg catgcgacga cgatcggcgt ggtggagggc gcgctcgggc agcgctggta cgacgtcacc gtgcacggga tggaggcgca cgccggcccg acgccgatgg cgctgcggcg cgacgcgctg ctggtggcgg ccgatctcgt gcgcgcggtg aacggcatcg cgctcgccca cccgccgcac gggcgcggca cggtcggctg gatcgacgtg catccgaatt cgcgcaacgt gattccgggc cgcgtgacgc tgacggtcga tctgcgcgcg gccgacgatg cgacgctcgc ggcgatggat gcggctctgc gtgcagcgtg tgccgatctc gcggcgacgg ccggcatgcc gatcgacgtc gagcaggtcg tgtacttcgc gcctcagccg ttcgacgcgg cgctcgtcga acaggtgcgc gcgggcgcga atgcgctggg cctgtcgtcg atgaacgtga tcagcggggc cggccacgat gccgtgtatc tcgcgcgtgt cgcgcccgcc gcgatgatct tcgtgccgtg caaggacggg atcagccaca acgaaatcga ggacgcggcg cctgccgatc tcgaggccgg ctgcaacgtg ctgctccatg cgatgctcgc cgcagccggc ctcgcggagg ggtacgcgcg atgagtaaga taggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaattc ttgaagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgc agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggccagga cccaacgctg cccgagatgc gccgcgtgcg gctgctggag atggcggacg cgatggatat gttctgccaa gggttggttt gcgcattcac agttctccgc aagaattgat tggctccaat tcttggagtg gtgaatccgt tagcgaggtg ccgccggctt ccattcaggt cgaggtggcc cggctccatg caccgcgacg caacgcgggg aggcagacaa ggtatagggc ggcgcctaca atccatgcca acccgttcca tgtgctcgcc gaggcggcat aaatcgccgt gacgatcagc ggtccagtga tcgaagttag gctggtaaga gccgcgagcg atccttgaag ctgtccctga tggtcgtcat ctacctgcct ggacagcatg gcctgcaacg cgggcatccc gatgccgccg gaagcgagaa gaatcataat ggggaaggcc atccagcctc gcgtcgcgaa cgccagcaag acgtagccca gcgcgtcggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaat