BuviA.12245.b
Amidase, hydantoinase/carbamoylase family
| CENTER ID: | BuviA.12245.b |
| ORGANISM: | Burkholderia vietnamiensis G4 |
| ASSOCIATED DISEASE: | |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | False |
| NIH RISK GROUP: | 3 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
IIIB |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| BuviA.12245.b.B1.GE39326 | Full length( BuviA.12245.b ) | 1 | 426 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Structures
5I4M
DEPOSITED: 2/12/2016
DETERMINATION: XRay
CLONE: BuviA.12245.b.B1.GE39326
PROTEIN: BuviA.12245.b.B1.PS02523
Citations by Others
| Zn2+-dependent enhancement of Atrazine biodegradation by Klebsiella variicola FH-1 | |
| J Zhang, X Wu, X Zhang, H Pan, JES Shearer- Journal of Hazardous, 2021 - Elsevier | |
| cited by: | 29 others |
| PDB: 5I4M | |
| A fundamental catalytic difference between zinc and manganese dependent enzymes revealed in a bacterial isatin hydrolase | |
| T Sommer, K Bjerregaard-Andersen, L Uribe- Scientific reports, 2018 - nature.com | |
| cited by: | 17 others |
| PDB: 5I4M | |
This list was obtained from Google Scholar searches using the Open Source tool https://github.com/ckreibich/scholar.py and each citation has been manually reviewed.
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| BV-BRC: | fig|269482.11.peg.910 |
| RefSeq: | YP_001114708.1 |
| UniProt: | A4JQA0 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MDAVSETAKR AALDTSIKVD GRRLWDSLME VAKIGATPKG GVCRLALTDL DKAARDLIVG WAKAAGCTVT VDTMGNVFMR RAGRVADAAP VVTGSHADSQ PTGGRFDGIY GVLGGLEVIR SLNDHGIETE HPVEVVIWTN EEGSRFAPAM VASGVFAGVF PLEYGLSRKD VDGKTIGEEL ARIGYAGDAP CGGRKLHAAF ELHIEQGPIL EAECKTIGVV TDAQGQRWYE ITFTGQEAHA GPTPMPRRRD ALLGASRVVD LVNRIGLDHA PYGCATVGMM QVHPNSRNVI PGRVFFTVDF RHPDDAVLAK MDAALRDGVA RIAADIGLDT ALEQIFYYAP IAFDSACVAA VRAAADRFGY SHRDIVSGAG HDACYLAQVA PTSMVFVPCI DGISHNEIED ATPAWIEAGA NVLLHAMLSR ACEPVS
NT Sequence
ATGGACGCGG TATCGGAAAC AGCGAAGCGG GCAGCATTGG ACACGTCGAT CAAGGTCGAC GGCAGGCGGT TGTGGGACAG CCTGATGGAG GTCGCGAAGA TCGGCGCGAC GCCGAAGGGC GGCGTGTGCC GTCTCGCGCT GACCGATCTC GACAAGGCCG CACGCGACCT GATCGTCGGT TGGGCGAAGG CAGCCGGCTG CACGGTGACG GTCGATACGA TGGGCAACGT GTTCATGCGG CGTGCGGGCC GCGTGGCCGA CGCGGCGCCG GTCGTGACCG GCTCGCACGC GGATTCGCAA CCGACCGGCG GCCGTTTCGA CGGCATCTAC GGCGTGCTCG GCGGCCTCGA GGTGATTCGG AGTCTGAACG ACCACGGGAT CGAGACCGAG CACCCGGTCG AGGTCGTGAT CTGGACCAAC GAGGAAGGCT CGCGCTTCGC GCCGGCCATG GTGGCGTCGG GCGTGTTCGC GGGCGTGTTT CCGCTCGAGT ACGGGCTGTC GCGCAAGGAC GTCGACGGCA AGACGATCGG CGAGGAACTC GCGCGGATCG GCTATGCGGG CGACGCGCCG TGCGGCGGCC GCAAGCTGCA CGCGGCGTTC GAGCTGCACA TCGAGCAGGG GCCGATACTC GAGGCCGAGT GCAAGACGAT CGGCGTCGTG ACCGATGCGC AGGGGCAGCG CTGGTACGAA ATCACGTTCA CCGGCCAGGA GGCGCACGCG GGGCCGACGC CGATGCCGCG CCGCCGCGAC GCGCTGCTCG GTGCATCGCG CGTGGTCGAT CTCGTGAACC GCATCGGCCT CGATCATGCG CCGTACGGCT GCGCGACGGT CGGGATGATG CAGGTGCATC CGAACTCGCG CAACGTGATT CCGGGCCGCG TGTTCTTCAC CGTCGATTTC CGCCATCCGG ACGACGCGGT GCTCGCGAAG ATGGACGCGG CACTGCGCGA CGGTGTAGCG CGCATCGCCG CCGACATCGG GCTCGACACC GCGCTCGAGC AGATTTTCTA CTACGCGCCC ATTGCGTTCG ACTCCGCGTG CGTGGCCGCG GTGCGCGCGG CGGCCGACCG CTTCGGCTAC TCGCATCGCG ACATCGTGTC GGGTGCCGGT CACGACGCGT GCTATCTCGC GCAGGTCGCG CCGACGTCGA TGGTGTTCGT GCCGTGCATC GACGGCATCA GTCACAACGA GATCGAGGAC GCGACGCCTG CGTGGATCGA GGCCGGCGCG AACGTGCTGC TGCACGCGAT GCTGTCGCGT GCATGCGAGC CGGTTTCA
Details for BuviA.12245.b.B1.GE39326
| HARVESTED ON: | 7/10/2015 |
| SEQUENCED ON: | 7/6/2015 |
| EXPECTED MW: | 46kDa |
| OBSERVED MW: | 44kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Too many to count (100+) |
| TOTAL EXPRESSION LEVEL: | Moderate Expression |
| SOLUBLE EXPRESSION LEVEL | High Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 97 |
Validated AA Sequence
MAHHHHHHMD AVSETAKRAA LDTSIKVDGR RLWDSLMEVA KIGATPKGGV CRLALTDLDK AARDLIVGWA KAAGCTVTVD TMGNVFMRRA GRVADAAPVV TGSHADSQPT GGRFDGIYGV LGGLEVIRSL NDHGIETEHP VEVVIWTNEE GSRFAPAMVA SGVFAGVFPL EYGLSRKDVD GKTIGEELAR IGYAGDAPCG GRKLHAAFEL HIEQGPILEA ECKTIGVVTD AQGQRWYEIT FTGQEAHAGP TPMPRRRDAL LGASRVVDLV NRIGLDHAPY GCATVGMMQV HPNSRNVIPG RVFFTVDFRH PDDAVLAKMD AALRDGVARI AADIGLDTAL EQIFYYAPIA FDSACVAAVR AAADRFGYSH RDIVSGAGHD ACYLAQVAPT SMVFVPCIDG ISHNEIEDAT PAWIEAGANV LLHA
Validated NT Sequence
atcgcgtgca gcagcacgtt cgcgccggcc tcgatccacg caggcgtcgc gtcctcgatc tcgttgtgac tgatgccgtc gatgcacggc acgaacacca tcgacgtcgg cgcgacctgc gcgagatagc acgcgtcgtg accggcaccc gacacgatgt cgcgatgcga gtagccgaag cggtcggccg ccgcgcgcac cgcggccacg cacgcggagt cgaacgcaat gggcgcgtag tagaaaatct gctcgagcgc ggtgtcgagc ccgatgtcgg cggcgatgcg cgctacaccg tcgcgcagtg ccgcgtccat cttcgcgagc accgcgtcgt ccggatggcg gaaatcgacg gtgaagaaca cgcggcccgg aatcacgttg cgcgagttcg gatgcacctg catcatcccg accgtcgcgc agccgtacgg cgcatgatcg aggccgatgc ggttcacgag atcgaccacg cgcgatgcac cgagcagcgc gtcgcggcgg cgcggcatcg gcgtcggccc cgcgtgcgcc tcctggccgg tgaacgtgat ttcgtaccag cgctgcccct gcgcatcggt cacgacgccg atcgtcttgc actcggcctc gagtatcggc ccctgctcga tgtgcagctc gaacgccgcg tgcagcttgc ggccgccgca cggcgcgtcg cccgcatagc cgatccgcgc gagttcctcg ccgatcgtct tgccgtcgac gtccttgcgc gacagcccgt actcgagcgg aaacacgccc gcgaacacgc ccgacgccac catggccggc gcgaagcgcg agccttcctc gttggtccag atcacgacct cgaccgggtg ctcggtctcg atcccgtggt cgttcagact ccgaatcacc tcgaggccgc cgagcacgcc gtagatgccg tcgaaacggc cgccggtcgg ttgcgaatcc gcgtgcgagc cggtcacgac cggcgccgcg tcggccacgc ggcccgcacg ccgcatgaac acgttgccca tcgtatcgac cgtcaccgtg cagccggctg ccttcgccca accgacgatc aggtcgcgtg cggccttgtc gagatcggtc agcgcgagac ggcacacgcc gcccttcggc gtcgcgccga tcttcgcgac ctccatcagg ctgtcccaca accgcctgcc gtcgaccttg atcgacgtgt ccaatgctgc ccgcttcgct gtttccgata ccgcgtccat atggtggtgg tggtggtgag ccat
Expected Protein Sequence
MAHHHHHHMD AVSETAKRAA LDTSIKVDGR RLWDSLMEVA KIGATPKGGV CRLALTDLDK AARDLIVGWA KAAGCTVTVD TMGNVFMRRA GRVADAAPVV TGSHADSQPT GGRFDGIYGV LGGLEVIRSL NDHGIETEHP VEVVIWTNEE GSRFAPAMVA SGVFAGVFPL EYGLSRKDVD GKTIGEELAR IGYAGDAPCG GRKLHAAFEL HIEQGPILEA ECKTIGVVTD AQGQRWYEIT FTGQEAHAGP TPMPRRRDAL LGASRVVDLV NRIGLDHAPY GCATVGMMQV HPNSRNVIPG RVFFTVDFRH PDDAVLAKMD AALRDGVARI AADIGLDTAL EQIFYYAPIA FDSACVAAVR AAADRFGYSH RDIVSGAGHD ACYLAQVAPT SMVFVPCIDG ISHNEIEDAT PAWIEAGANV LLHAMLSRAC EPVS
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggacg cggtatcgga aacagcgaag cgggcagcat tggacacgtc gatcaaggtc gacggcaggc ggttgtggga cagcctgatg gaggtcgcga agatcggcgc gacgccgaag ggcggcgtgt gccgtctcgc gctgaccgat ctcgacaagg ccgcacgcga cctgatcgtc ggttgggcga aggcagccgg ctgcacggtg acggtcgata cgatgggcaa cgtgttcatg cggcgtgcgg gccgcgtggc cgacgcggcg ccggtcgtga ccggctcgca cgcggattcg caaccgaccg gcggccgttt cgacggcatc tacggcgtgc tcggcggcct cgaggtgatt cggagtctga acgaccacgg gatcgagacc gagcacccgg tcgaggtcgt gatctggacc aacgaggaag gctcgcgctt cgcgccggcc atggtggcgt cgggcgtgtt cgcgggcgtg tttccgctcg agtacgggct gtcgcgcaag gacgtcgacg gcaagacgat cggcgaggaa ctcgcgcgga tcggctatgc gggcgacgcg ccgtgcggcg gccgcaagct gcacgcggcg ttcgagctgc acatcgagca ggggccgata ctcgaggccg agtgcaagac gatcggcgtc gtgaccgatg cgcaggggca gcgctggtac gaaatcacgt tcaccggcca ggaggcgcac gcggggccga cgccgatgcc gcgccgccgc gacgcgctgc tcggtgcatc gcgcgtggtc gatctcgtga accgcatcgg cctcgatcat gcgccgtacg gctgcgcgac ggtcgggatg atgcaggtgc atccgaactc gcgcaacgtg attccgggcc gcgtgttctt caccgtcgat ttccgccatc cggacgacgc ggtgctcgcg aagatggacg cggcactgcg cgacggtgta gcgcgcatcg ccgccgacat cgggctcgac accgcgctcg agcagatttt ctactacgcg cccattgcgt tcgactccgc gtgcgtggcc gcggtgcgcg cggcggccga ccgcttcggc tactcgcatc gcgacatcgt gtcgggtgcc ggtcacgacg cgtgctatct cgcgcaggtc gcgccgacgt cgatggtgtt cgtgccgtgc atcgacggca tcagtcacaa cgagatcgag gacgcgacgc ctgcgtggat cgaggccggc gcgaacgtgc tgctgcacgc gatgctgtcg cgtgcatgcg agccggtttc atgagtaaga taggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaattc ttgaagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgc agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggccagga cccaacgctg cccgagatgc gccgcgtgcg gctgctggag atggcggacg cgatggatat gttctgccaa gggttggttt gcgcattcac agttctccgc aagaattgat tggctccaat tcttggagtg gtgaatccgt tagcgaggtg ccgccggctt ccattcaggt cgaggtggcc cggctccatg caccgcgacg caacgcgggg aggcagacaa ggtatagggc ggcgcctaca atccatgcca acccgttcca tgtgctcgcc gaggcggcat aaatcgccgt gacgatcagc ggtccagtga tcgaagttag gctggtaaga gccgcgagcg atccttgaag ctgtccctga tggtcgtcat ctacctgcct ggacagcatg gcctgcaacg cgggcatccc gatgccgccg gaagcgagaa gaatcataat ggggaaggcc atccagcctc gcgtcgcgaa cgccagcaag acgtagccca gcgcgtcggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaat