BuviA.11726.a

N-acetylmuramoyl-L-alanine amidase

CENTER ID: BuviA.11726.a
ORGANISM: Burkholderia vietnamiensis G4
ASSOCIATED DISEASE:
CURRENT STATUS: soluble
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BuviA.11726.a.B2.GE35347 Mature protein( BuviA.11726.a ) 48 505
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|269482.11.peg.6912
RefSeq: YP_001120483.1
UniProt: A4JH95

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSRKMLIKPF RSIESAATAT HNWRRRQILR AGASTLVLGL VAPRVAHASS VLGVRVWPAR DYTRVTIESD QPLQNSQQLL QGPDRLVVDL NGLDLDQALR DLVSKIAPND PQIQSVRVGQ YQPHVVRMVF DLKGSVKPQV FTLPPVGTYK YRLVFDLYPA VAPDPLTDLI AQTERKEQAL NDSARAQQMQ PPSRLTGPAT PPAAADNSDA FFQRFAQNTP AAPRTPQAAP STPAKPAVKP PPVIARRDDS DDDADTYKFT APKSGKGGTM RLLTVAIDPG HGGEDPGAIG GAGTYEKHVA LDIAKKLRAK IDGAPNMRAM MTRDADFFVP LNVRVQKARR VGADLFVSIH ADAFTTPSAS GSSVFALSDH GASSAAARWL ANKENASDLI GGINIKTQDA AVSRALFDMS TTAQIRDSLR YGNYVLKEVG GINKLHKGSV EQAGFAVLKA PDIPSILVET AFISNPDEER RLNDDSYREE MADAIFRGIK RYFAANPPLA KNRMA
NT Sequence
ATGTCTCGAA AGATGTTGAT CAAACCGTTC CGCTCGATCG AATCGGCGGC CACCGCGACG CACAACTGGC GGCGCCGCCA GATCCTGCGC GCCGGTGCGT CGACGCTGGT GCTCGGTCTC GTCGCGCCGC GCGTCGCGCA CGCATCTTCG GTGCTCGGCG TGCGCGTCTG GCCCGCGCGC GACTACACGC GCGTCACGAT CGAATCCGAT CAGCCGCTGC AGAACAGCCA GCAGCTGCTG CAGGGGCCGG ACCGCCTCGT CGTCGACCTG AACGGCCTCG ATCTCGACCA GGCGCTGCGC GACCTCGTGT CGAAGATCGC GCCGAACGAT CCGCAGATCC AGTCGGTGCG CGTCGGCCAG TATCAGCCGC ACGTGGTGCG GATGGTGTTC GACCTGAAGG GCTCGGTGAA ACCGCAGGTG TTCACGCTGC CGCCGGTGGG CACCTACAAG TACCGGCTCG TGTTCGACCT GTATCCGGCG GTCGCGCCCG ATCCGCTGAC CGACCTGATC GCACAGACCG AACGCAAGGA GCAGGCGCTG AACGACAGCG CGCGCGCGCA ACAGATGCAG CCGCCGTCGA GGCTCACGGG CCCGGCCACG CCGCCCGCCG CGGCCGACAA CAGCGACGCG TTCTTCCAGC GCTTCGCGCA GAACACGCCG GCCGCGCCGC GCACGCCGCA GGCCGCGCCT TCGACGCCGG CGAAGCCGGC CGTCAAGCCG CCGCCCGTCA TCGCGCGCCG CGACGACAGC GACGACGACG CCGACACCTA CAAGTTCACC GCGCCGAAAT CCGGCAAGGG CGGCACGATG CGGCTGCTGA CGGTCGCGAT CGATCCGGGC CACGGCGGCG AGGATCCGGG CGCGATCGGC GGCGCAGGCA CCTACGAGAA GCACGTCGCG CTCGACATCG CGAAGAAGCT GCGCGCGAAG ATCGACGGCG CGCCCAACAT GCGCGCGATG ATGACCCGCG ACGCCGACTT CTTCGTGCCG CTGAACGTGC GCGTGCAGAA GGCGCGCCGC GTCGGCGCGG ACCTGTTCGT GTCGATCCAC GCGGACGCGT TCACGACGCC GTCCGCGAGC GGCTCGTCGG TGTTCGCGCT GTCCGACCAC GGCGCGTCGA GCGCCGCCGC GCGCTGGCTC GCGAACAAGG AAAACGCATC CGACCTGATC GGCGGCATCA ACATCAAGAC GCAGGACGCG GCCGTGAGCC GCGCGCTGTT CGACATGTCG ACCACCGCGC AGATCCGCGA TTCACTGCGC TACGGCAACT ACGTACTGAA GGAAGTCGGC GGCATCAACA AGCTGCACAA GGGCTCGGTC GAGCAGGCCG GGTTCGCGGT GCTGAAGGCG CCCGACATTC CGTCGATCCT GGTCGAGACC GCGTTCATCA GCAACCCGGA CGAAGAACGC CGGCTCAACG ACGACAGCTA TCGCGAGGAA ATGGCCGACG CGATCTTCCG CGGCATCAAG CGCTACTTCG CGGCCAATCC GCCGCTCGCG AAGAACCGGA TGGCC
Details for BuviA.11726.a.B2.GE35347
HARVESTED ON: 5/16/2012
SEQUENCED ON: 5/15/2012
EXPECTED MW: 50kDa
OBSERVED MW: 50kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 100
PERCENT COVERAGE: 44
Validated AA Sequence
MVFDLKGSVK PQVFTLPPVG TYKYRLVFDL YPAVAPDPLT DLIAQTERKE QALNDSARAQ QMQPPSRLTG PATPPAAADN SDAFFQRFAQ NTPAAPRTPQ AAPSTPAKPA VKPPPVIARR DDSDDDADTY KFTAPKSGKG GTMRLLTVAI DPGHGGEDPG AIGGAGTYEK HVALDIAKKL RAKIDGAPNM RAMMTRDADF FVPLNVRVQ
Validated NT Sequence
ttctgcacgc gcacgttcag cggcacgaag aagtcggcgt cgcgggtcat catcgcgcgc atgttgggcg cgccgtcgat cttcgcgcgc agcttcttcg cgatgtcgag cgcgacgtgc ttctcgtagg tgcctgcgcc gccgatcgcg cccggatcct cgccgccgtg gcccggatcg atcgcgaccg tcagcagccg catcgtgccg cccttgccgg atttcggcgc ggtgaacttg taggtgtcgg cgtcgtcgtc gctgtcgtcg cggcgcgcga tgacgggcgg cggcttgacg gccggcttcg ccggcgtcga aggcgcggcc tgcggcgtgc gcggcgcggc cggcgtgttc tgcgcgaagc gctggaagaa cgcgtcgctg ttgtcggccg cggcgggcgg cgtggccggg cccgtgagcc tcgacggcgg ctgcatctgt tgcgcgcgcg cgctgtcgtt cagcgcctgc tccttgcgtt cggtctgtgc gatcaggtcg gtcagcggat cgggcgcgac cgccggatac aggtcgaaca cgagccggta cttgtaggtg cccaccggcg gcagcgtgaa cacctgcggt ttcaccgagc ccttcaggtc gaacaccatc cgcaccacgt gcggctgata ctggccgacg cgcaccgact ggatctgcgg atcgttcggc gcgatcttcg acacgaggtc gcgcagcgcc tggtcgagat cgaggccgtt caggtcgacg acgaggcggt ccggcccctg cagcagctgc tggctgttct gcagcggctg atcggattcg atcgtgacgc gcgtgtagtc gcgcgcgggc cagacgcgca cgccgagcac cgaaga
Expected Protein Sequence
MAHHHHHHMA SSVLGVRVWP ARDYTRVTIE SDQPLQNSQQ LLQGPDRLVV DLNGLDLDQA LRDLVSKIAP NDPQIQSVRV GQYQPHVVRM VFDLKGSVKP QVFTLPPVGT YKYRLVFDLY PAVAPDPLTD LIAQTERKEQ ALNDSARAQQ MQPPSRLTGP ATPPAAADNS DAFFQRFAQN TPAAPRTPQA APSTPAKPAV KPPPVIARRD DSDDDADTYK FTAPKSGKGG TMRLLTVAID PGHGGEDPGA IGGAGTYEKH VALDIAKKLR AKIDGAPNMR AMMTRDADFF VPLNVRVQKA RRVGADLFVS IHADAFTTPS ASGSSVFALS DHGASSAAAR WLANKENASD LIGGINIKTQ DAAVSRALFD MSTTAQIRDS LRYGNYVLKE VGGINKLHKG SVEQAGFAVL KAPDIPSILV ETAFISNPDE ERRLNDDSYR EEMADAIFRG IKRYFAANPP LAKNRMA
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggcat cttcggtgct cggcgtgcgc gtctggcccg cgcgcgacta cacgcgcgtc acgatcgaat ccgatcagcc gctgcagaac agccagcagc tgctgcaggg gccggaccgc ctcgtcgtcg acctgaacgg cctcgatctc gaccaggcgc tgcgcgacct cgtgtcgaag atcgcgccga acgatccgca gatccagtcg gtgcgcgtcg gccagtatca gccgcacgtg gtgcggatgg tgttcgacct gaagggctcg gtgaaaccgc aggtgttcac gctgccgccg gtgggcacct acaagtaccg gctcgtgttc gacctgtatc cggcggtcgc gcccgatccg ctgaccgacc tgatcgcaca gaccgaacgc aaggagcagg cgctgaacga cagcgcgcgc gcgcaacaga tgcagccgcc gtcgaggctc acgggcccgg ccacgccgcc cgccgcggcc gacaacagcg acgcgttctt ccagcgcttc gcgcagaaca cgccggccgc gccgcgcacg ccgcaggccg cgccttcgac gccggcgaag ccggccgtca agccgccgcc cgtcatcgcg cgccgcgacg acagcgacga cgacgccgac acctacaagt tcaccgcgcc gaaatccggc aagggcggca cgatgcggct gctgacggtc gcgatcgatc cgggccacgg cggcgaggat ccgggcgcga tcggcggcgc aggcacctac gagaagcacg tcgcgctcga catcgcgaag aagctgcgcg cgaagatcga cggcgcgccc aacatgcgcg cgatgatgac ccgcgacgcc gacttcttcg tgccgctgaa cgtgcgcgtg cagaaggcgc gccgcgtcgg cgcggacctg ttcgtgtcga tccacgcgga cgcgttcacg acgccgtccg cgagcggctc gtcggtgttc gcgctgtccg accacggcgc gtcgagcgcc gccgcgcgct ggctcgcgaa caaggaaaac gcatccgacc tgatcggcgg catcaacatc aagacgcagg acgcggccgt gagccgcgcg ctgttcgaca tgtcgaccac cgcgcagatc cgcgattcac tgcgctacgg caactacgta ctgaaggaag tcggcggcat caacaagctg cacaagggct cggtcgagca ggccgggttc gcggtgctga aggcgcccga cattccgtcg atcctggtcg agaccgcgtt catcagcaac ccggacgaag aacgccggct caacgacgac agctatcgcg aggaaatggc cgacgcgatc ttccgcggca tcaagcgcta cttcgcggcc aatccgccgc tcgcgaagaa ccggatggcc tgagtaagat aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaattct tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgca gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg cagctgcggt aaagctcatc agcgtggtcg tgaagcgatt cacagatgtc tgcctgttca tccgcgtcca gctcgttgag tttctccaga agcgttaatg tctggcttct gataaagcgg gccatgttaa gggcggtttt ttcctgtttg gtcactgatg cctccgtgta agggggattt ctgttcatgg gggtaatgat accgatgaaa cgagagagga tgctcacgat acgggttact gatgatgaac atgcccggtt actggaacgt tgtgagggta aacaactggc ggtatggatg cggcgggacc agagaaaaat cactcagggt caatgccagc gcttcgttaa tacagatgta ggtgttccac agggtagcca gcagcatcct gcgatgcaga tccggaacat aatggtgcag ggcgctgact tccgcgtttc cagactttac gaaacacgga aaccgaagac cattcatgtt gttgctcagg tcgcagacgt tttgcagcag cagtcgcttc acgttcgctc gcgtatcggt gattcattct gctaaccagt aaggcaaccc cgccagccta gccgggtcct caacgacagg agcacgatca tgcgcacccg tggccaggac ccaacgctgc ccgagatgcg ccgcgtgcgg ctgctggaga tggcggacgc gatggatatg ttctgccaag ggttggtttg cgcattcaca gttctccgca agaattgatt ggctccaatt cttggagtgg tgaatccgtt agcgaggtgc cgccggcttc cattcaggtc gaggtggccc ggctccatgc accgcgacgc aacgcgggga ggcagacaag gtatagggcg gcgcctacaa tccatgccaa cccgttccat gtgctcgccg aggcggcata aatcgccgtg acgatcagcg gtccagtgat cgaagttagg ctggtaagag ccgcgagcga tccttgaagc tgtccctgat ggtcgtcatc tacctgcctg gacagcatgg cctgcaacgc gggcatcccg atgccgccgg aagcgagaag aatcataatg gggaaggcca tccagcctcg cgtcgcgaac gccagcaaga cgtagcccag cgcgtcggcc gccatgccgg cgataatggc ctgcttctcg ccgaaacgtt tggtggcggg accagtgacg aaggcttgag cgagggcgtg caagattccg aataccgcaa gcgacaggcc gatcatcgtc gcgctccagc gaaagcggtc ctcgccgaaa atgacccaga gcgctgccgg cacctgtcct acgagttgca tgataaagaa gacagtcata agtgcggcga cgatagtcat gccccgcgcc caccggaagg agctgactgg gttgaaggct ctcaagggca tcggtcgacg ctctccctta tgcgactcct gcattaggaa gcagcccagt agtaggttga ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg cccaacagtc ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg agcccgaagt ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca accgcacctg tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gatcgagatc tcgatcccgc gaaat