BuviA.10647.a

Peptidoglycan synthetase FtsI

CENTER ID: BuviA.10647.a
ORGANISM: Burkholderia vietnamiensis G4
ASSOCIATED DISEASE:
CURRENT STATUS: purified
COMMUNITY REQUEST: False
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BuviA.10647.a.B2.GE39324 Mature protein( BuviA.10647.a ) 45 616
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|269482.11.peg.4725
RefSeq: YP_001118376.1
UniProt: A4JB88

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MKPSHKRQDV KFSSSPVLGV HLPMWRSKLV VFLLFMAFVA LTARAFWIQG PGNAFYQKQG ESRYQRTIEL PATRGKILDR NGLVLATSLP VRAIWAIPDA VPDDLDAAKI NQLGKLLGMT PKELRIKLSE DKGFVYVKRQ VPLDVADKVA ALDIPGIYQR NEYKRFYPEG EITAHLIGFT NVEDEGQEGV ELGDQKSLSG TSGVRRVIKD RMGHIVEDVA EQIPPHNGTD VDLSIDSKIQ YIAYANLKAA VEKFKAKAGA AMVVDVRTGE VLALVNYPTY NPNDRTRLTG EQLRNRILTD VFEPGSIMKP FTVSLALDLH RVTPNTLVET GNGHFVLDGA PITDDAGFGT LTVGGVIQKS SNIGATKIAM TMRPEEMWNM YTSIGLGQAP KVGFPGAAAG RLRPWKSWRR IEQATMSYGY GLSVSLFQLA RAYTAIAHDG ELMPVTIFKT DPNQQITGTQ VFTPTTAREV RAMLETVVAP GGTSPDAAVP GYRVGGKSGT AYKHEGHGYT RKYRASFVGM APMPNPRIVV AVSVDEPTAG SHFGGQVSGP VFSAIAGDTM RALNVPPNMP IKQLVVSGDA APAAPAASGP QKLAGSGHAK HMIVSSTTRN SPGVVR
NT Sequence
ATGAAGCCGT CGCACAAGCG CCAGGACGTG AAATTCTCGT CGAGCCCCGT GCTCGGCGTG CACCTGCCGA TGTGGCGCTC GAAGCTCGTC GTGTTCCTGC TGTTCATGGC GTTCGTCGCG CTGACCGCGC GCGCGTTCTG GATCCAGGGC CCCGGCAACG CGTTCTACCA GAAGCAGGGC GAGAGCCGCT ATCAGCGCAC GATCGAGCTG CCGGCCACGC GCGGCAAGAT CCTCGACCGC AACGGGCTCG TGCTCGCGAC GAGCCTGCCC GTGCGCGCGA TCTGGGCCAT TCCCGACGCC GTGCCGGACG ACCTCGACGC CGCCAAGATC AACCAGCTCG GCAAGCTGCT CGGCATGACG CCGAAGGAGC TGCGCATCAA GCTGTCGGAA GACAAGGGCT TCGTCTACGT GAAGCGTCAG GTGCCGCTCG ACGTCGCCGA CAAGGTCGCC GCGCTCGACA TCCCGGGCAT CTATCAGCGC AACGAGTACA AGCGCTTCTA TCCGGAAGGC GAGATCACCG CCCATCTGAT CGGCTTCACG AACGTCGAGG ACGAAGGGCA GGAGGGCGTC GAGCTCGGCG ACCAGAAGTC GCTGTCCGGC ACGTCCGGCG TGCGTCGCGT GATCAAGGAC CGGATGGGGC ACATCGTCGA GGACGTCGCC GAACAGATTC CGCCGCACAA CGGCACCGAC GTCGACCTGT CGATCGACAG CAAGATCCAG TACATCGCGT ACGCGAACCT GAAGGCTGCC GTCGAGAAGT TCAAGGCCAA GGCCGGCGCG GCGATGGTCG TCGACGTGCG CACCGGCGAA GTGCTCGCGC TGGTCAACTA TCCGACCTAC AACCCGAACG ACCGCACGCG CCTGACCGGC GAGCAGCTGC GCAACCGGAT CCTGACCGAC GTGTTCGAGC CGGGCTCGAT CATGAAGCCG TTCACCGTGT CGCTCGCGCT CGACCTGCAT CGCGTGACGC CGAACACGCT CGTCGAGACG GGCAACGGGC ACTTCGTGCT CGACGGCGCG CCGATCACCG ACGATGCGGG CTTCGGCACG CTGACGGTCG GCGGTGTGAT CCAGAAGTCG AGCAACATCG GTGCGACCAA GATCGCGATG ACGATGCGGC CCGAGGAAAT GTGGAACATG TATACGAGCA TCGGCCTCGG CCAGGCGCCG AAGGTCGGCT TCCCCGGCGC CGCGGCCGGC CGGCTGCGCC CGTGGAAGAG CTGGCGCCGC ATCGAGCAGG CGACGATGTC GTACGGCTAC GGGCTGTCGG TATCGCTGTT CCAGCTGGCG CGCGCGTACA CCGCGATCGC GCACGACGGC GAACTGATGC CGGTGACCAT TTTCAAGACC GACCCCAATC AGCAGATCAC GGGCACGCAG GTATTCACGC CGACCACCGC GCGCGAAGTG CGCGCGATGC TCGAGACCGT CGTCGCGCCG GGCGGCACGT CGCCGGACGC CGCGGTGCCC GGCTATCGCG TCGGCGGCAA GAGCGGTACC GCCTACAAGC ACGAAGGTCA TGGCTACACG CGCAAGTACC GCGCGTCGTT CGTCGGGATG GCGCCGATGC CGAATCCGCG CATCGTGGTC GCGGTGTCGG TGGACGAGCC GACCGCAGGC AGCCACTTCG GCGGGCAGGT CTCGGGCCCC GTATTTTCGG CGATCGCGGG TGATACGATG CGCGCGCTGA ACGTGCCGCC GAACATGCCG ATCAAGCAGC TCGTCGTATC GGGCGACGCC GCGCCGGCGG CGCCCGCCGC GTCGGGGCCG CAAAAGCTCG CCGGCAGCGG CCACGCGAAG CATATGATCG TTTCCAGCAC TACACGTAAT TCACCAGGAG TTGTTCGA
Details for BuviA.10647.a.B2.GE39324
HARVESTED ON: 7/10/2015
SEQUENCED ON: 7/6/2015
EXPECTED MW: 63kDa
OBSERVED MW: 63kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY: 68
PERCENT COVERAGE: 90
Validated AA Sequence
MAHHHHHHMA FWIQGPGNAF YQKQGESRYQ RTIELPATRG KILDRNGLVL ATSLPVRAIW AIPDAVPDDL DAAKINQLGK LLGMTPKELR IKLSEDKGFV YVKRQVPLDV ADKVAALDIP GIYQRNEYKR FYPEGEITAH LIGFTNVEDE GQEGVELGDQ KSLSGTSGVR RVIKDRMGHI VEDVAEQIPP HNGTDVDLSI DSKIQYIAYA NLKAAVEKFK AKAGAAMVVD VRTGEVLALV NYPTYNPNDR TRLTXEQLXN RILTDVFEPG SIMKPFTVSL ALDLHRVTPN TLVETGNGHF VLDXRADHRR CGXRHADGRR CDPEVEQHRC DQDRDDDAAR GNVEHVYEHR PRPGAEGRLP RRRGRPAAPV EELAPHRAGD DVVRLRAVGI AVPAGARVHR DRARRRTDAG DHFQDRPQSA DHGHAGIHAD HRARSARDAR DRRRAGRHVA GRRGARLSRR RQERYRLQAR RSWLHAQVPR VVRRDGADAE SAHRGRGVGG RADRRQPLRR AGLGPRIFGD RG
Validated NT Sequence
gtagtgctgg aaacgatcat atgcttcgcg tggccgctgc cggcgagctt ttgcggcccc gacgcggcgg gcgccgccgg cgcggcgtcg cccgatacga cgagctgctt gatcggcatg ttcggcggca cgttcagcgc gcgcatcgta tcacccgcga tcgccgaaaa tacggggccc gagacctgcc cgccgaagtg gctgcctgcg gtcggctcgt ccaccgacac cgcgaccacg atgcgcggat tcggcatcgg cgccatcccg acgaacgacg cgcggtactt gcgcgtgtag ccatgacctt cgtgcttgta ggcggtaccg ctcttgccgc cgacgcgata gccgggcacc gcggcgtccg gcgacgtgcc gcccggcgcg acgacggtct cgagcatcgc gcgcacttcg cgcgcggtgg tcggcgtgaa tacctgcgtg cccgtgatct gctgattggg gtcggtcttg aaaatggtca ccggcatcag ttcgccgtcg tgcgcgatcg cggtgtacgc gcgcgccagc tggaacagcg ataccgacag cccgtagccg tacgacatcg tcgcctgctc gatgcggcgc cagctcttcc acgggcgcag ccggccggcc gcggcgccgg ggaagccgac cttcggcgcc tggccgaggc cgatgctcgt atacatgttc cacatttcct cgggccgcat cgtcatcgcg atcttggtcg caccgatgtt gctcgacttc tggatcacac cgccgaccgt cagcgtgccg annnccgcat cgtcggtgat cggcgcgcnn gtcgagcacg aagtgcccgt tgcccgtctc gacgagcgtg ttcggcgtca cgcgatgcag gtcgagcgcg agcgacacgg tgaacggctt catgatcgag cccggctcga acacgtcggt caggatccgg ttnnncagct gctcgncggt caggcgcgtg cggtcgttcg ggttgtaggt cggatagttg accagcgcga gcacttcgcc ggtgcgcacg tcgacgacca tcgccgcgcc ggccttggcc ttgaacttct cgacggcagc cttcaggttc gcgtacgcga tgtactggat cttgctgtcg atcgacaggt cgacgtcggt gccgttgtgc ggcggaatct gttcggcgac gtcctcgacg atgtgcccca tccggtcctt gatcacgcga cgcacgccgg acgtgccgga cagcgacttc tggtcgccga gctcgacgcc ctcctgccct tcgtcctcga cgttcgtgaa gccgatcaga tgggcggtga tctcgccttc cggatagaag cgcttgtact cgttgcgctg atagatgccc gggatgtcga gcgcggcgac cttgtcggcg acgtcgagcg gcacctgacg cttcacgtag acgaagccct tgtcttccga cagcttgatg cgcagctcct tcggcgtcat gccgagcagc ttgccgagct ggttgatctt ggcggcgtcg aggtcgtccg gcacggcgtc gggaatggcc cagatcgcgc gcacgggcag gctcgtcgcg agcacgagcc cgttgcggtc gaggatcttg ccgcgcgtgg ccggcagctc gatcgtgcgc tgatagcggc tctcgccctg cttctggtag aacgcgttgc cggggccctg gatccagaac gccatatggt ggtggtggtg gtgagccat
Expected Protein Sequence
MAHHHHHHAF WIQGPGNAFY QKQGESRYQR TIELPATRGK ILDRNGLVLA TSLPVRAIWA IPDAVPDDLD AAKINQLGKL LGMTPKELRI KLSEDKGFVY VKRQVPLDVA DKVAALDIPG IYQRNEYKRF YPEGEITAHL IGFTNVEDEG QEGVELGDQK SLSGTSGVRR VIKDRMGHIV EDVAEQIPPH NGTDVDLSID SKIQYIAYAN LKAAVEKFKA KAGAAMVVDV RTGEVLALVN YPTYNPNDRT RLTGEQLRNR ILTDVFEPGS IMKPFTVSLA LDLHRVTPNT LVETGNGHFV LDGAPITDDA GFGTLTVGGV IQKSSNIGAT KIAMTMRPEE MWNMYTSIGL GQAPKVGFPG AAAGRLRPWK SWRRIEQATM SYGYGLSVSL FQLARAYTAI AHDGELMPVT IFKTDPNQQI TGTQVFTPTT AREVRAMLET VVAPGGTSPD AAVPGYRVGG KSGTAYKHEG HGYTRKYRAS FVGMAPMPNP RIVVAVSVDE PTAGSHFGGQ VSGPVFSAIA GDTMRALNVP PNMPIKQLVV SGDAAPAAPA ASGPQKLAGS GHAKHMIVSS TTRNSPGVVR
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgcgttct ggatccaggg ccccggcaac gcgttctacc agaagcaggg cgagagccgc tatcagcgca cgatcgagct gccggccacg cgcggcaaga tcctcgaccg caacgggctc gtgctcgcga cgagcctgcc cgtgcgcgcg atctgggcca ttcccgacgc cgtgccggac gacctcgacg ccgccaagat caaccagctc ggcaagctgc tcggcatgac gccgaaggag ctgcgcatca agctgtcgga agacaagggc ttcgtctacg tgaagcgtca ggtgccgctc gacgtcgccg acaaggtcgc cgcgctcgac atcccgggca tctatcagcg caacgagtac aagcgcttct atccggaagg cgagatcacc gcccatctga tcggcttcac gaacgtcgag gacgaagggc aggagggcgt cgagctcggc gaccagaagt cgctgtccgg cacgtccggc gtgcgtcgcg tgatcaagga ccggatgggg cacatcgtcg aggacgtcgc cgaacagatt ccgccgcaca acggcaccga cgtcgacctg tcgatcgaca gcaagatcca gtacatcgcg tacgcgaacc tgaaggctgc cgtcgagaag ttcaaggcca aggccggcgc ggcgatggtc gtcgacgtgc gcaccggcga agtgctcgcg ctggtcaact atccgaccta caacccgaac gaccgcacgc gcctgaccgg cgagcagctg cgcaaccgga tcctgaccga cgtgttcgag ccgggctcga tcatgaagcc gttcaccgtg tcgctcgcgc tcgacctgca tcgcgtgacg ccgaacacgc tcgtcgagac gggcaacggg cacttcgtgc tcgacggcgc gccgatcacc gacgatgcgg gcttcggcac gctgacggtc ggcggtgtga tccagaagtc gagcaacatc ggtgcgacca agatcgcgat gacgatgcgg cccgaggaaa tgtggaacat gtatacgagc atcggcctcg gccaggcgcc gaaggtcggc ttccccggcg ccgcggccgg ccggctgcgc ccgtggaaga gctggcgccg catcgagcag gcgacgatgt cgtacggcta cgggctgtcg gtatcgctgt tccagctggc gcgcgcgtac accgcgatcg cgcacgacgg cgaactgatg ccggtgacca ttttcaagac cgaccccaat cagcagatca cgggcacgca ggtattcacg ccgaccaccg cgcgcgaagt gcgcgcgatg ctcgagaccg tcgtcgcgcc gggcggcacg tcgccggacg ccgcggtgcc cggctatcgc gtcggcggca agagcggtac cgcctacaag cacgaaggtc atggctacac gcgcaagtac cgcgcgtcgt tcgtcgggat ggcgccgatg ccgaatccgc gcatcgtggt cgcggtgtcg gtggacgagc cgaccgcagg cagccacttc ggcgggcagg tctcgggccc cgtattttcg gcgatcgcgg gtgatacgat gcgcgcgctg aacgtgccgc cgaacatgcc gatcaagcag ctcgtcgtat cgggcgacgc cgcgccggcg gcgcccgccg cgtcggggcc gcaaaagctc gccggcagcg gccacgcgaa gcatatgatc gtttccagca ctacacgtaa ttcaccagga gttgttcgat gagtaagata ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaattctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgcag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agctgcggta aagctcatca gcgtggtcgt gaagcgattc acagatgtct gcctgttcat ccgcgtccag ctcgttgagt ttctccagaa gcgttaatgt ctggcttctg ataaagcggg ccatgttaag ggcggttttt tcctgtttgg tcactgatgc ctccgtgtaa gggggatttc tgttcatggg ggtaatgata ccgatgaaac gagagaggat gctcacgata cgggttactg atgatgaaca tgcccggtta ctggaacgtt gtgagggtaa acaactggcg gtatggatgc ggcgggacca gagaaaaatc actcagggtc aatgccagcg cttcgttaat acagatgtag gtgttccaca gggtagccag cagcatcctg cgatgcagat ccggaacata atggtgcagg gcgctgactt ccgcgtttcc agactttacg aaacacggaa accgaagacc attcatgttg ttgctcaggt cgcagacgtt ttgcagcagc agtcgcttca cgttcgctcg cgtatcggtg attcattctg ctaaccagta aggcaacccc gccagcctag ccgggtcctc aacgacagga gcacgatcat gcgcacccgt ggccaggacc caacgctgcc cgagatgcgc cgcgtgcggc tgctggagat ggcggacgcg atggatatgt tctgccaagg gttggtttgc gcattcacag ttctccgcaa gaattgattg gctccaattc ttggagtggt gaatccgtta gcgaggtgcc gccggcttcc attcaggtcg aggtggcccg gctccatgca ccgcgacgca acgcggggag gcagacaagg tatagggcgg cgcctacaat ccatgccaac ccgttccatg tgctcgccga ggcggcataa atcgccgtga cgatcagcgg tccagtgatc gaagttaggc tggtaagagc cgcgagcgat ccttgaagct gtccctgatg gtcgtcatct acctgcctgg acagcatggc ctgcaacgcg ggcatcccga tgccgccgga agcgagaaga atcataatgg ggaaggccat ccagcctcgc gtcgcgaacg ccagcaagac gtagcccagc gcgtcggccg ccatgccggc gataatggcc tgcttctcgc cgaaacgttt ggtggcggga ccagtgacga aggcttgagc gagggcgtgc aagattccga ataccgcaag cgacaggccg atcatcgtcg cgctccagcg aaagcggtcc tcgccgaaaa tgacccagag cgctgccggc acctgtccta cgagttgcat gataaagaag acagtcataa gtgcggcgac gatagtcatg ccccgcgccc accggaagga gctgactggg ttgaaggctc tcaagggcat cggtcgacgc tctcccttat gcgactcctg cattaggaag cagcccagta gtaggttgag gccgttgagc accgccgccg caaggaatgg tgcatgcaag gagatggcgc ccaacagtcc cccggccacg gggcctgcca ccatacccac gccgaaacaa gcgctcatga gcccgaagtg gcgagcccga tcttccccat cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg atcgagatct cgatcccgcg aaat