SSGCID: Target Page

BuviA.10100.b

Protease Do

CENTER ID:	BuviA.10100.b
ORGANISM:	Burkholderia vietnamiensis G4
ASSOCIATED DISEASE:
CURRENT STATUS:	purified
COMMUNITY REQUEST:	False
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BuviA.10100.b.B2.GE39321	Mature protein( BuviA.10100.b )		30	494

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BuviA.10100.b.B2.PS02532	Mature protein( BuviA.10100.b )		30	494

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|269482.11.peg.6976
RefSeq:	YP_001120557.1
UniProt:	A4JHG9

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MNTRFLARGA VAVAVAAALS AGYVAGTRHA DPQIITPAQA AALMPAEAAA KTGIPDFSGL VETYGPAVVN ISAKHVVKQV SRRVQQPQLP MDPSDPFYQF FKHFYGQVPG MGGDAQPDDQ PSASLGSGFI VSPDGYILTN AHVIDGANVV TVKLTDKREY KAKVVGSDKQ SDVAVLKIDA SGLPTVKIGD PARSKVGQWV VAIGSPYGFD NTVTSGIISA KSRALPDENY TPFIQTDVPV NPGNSGGPLF NLQGEVIGIN SMIYSQTGGF QGLSFAIPIN EAIKVKDELV KTGHVSRGRL GVAVQGLNQT LASSFGLPKP DGALVSSVDP SGPAAKAGLQ PGDVILAVNG SPVADSTSLP AQIASLKPGS QADLQIWRDK SKKSISVTLG AMTDAKLASN DGGPVEQGRL GVAVRPLTPQ ERSATNLSHG LMVQQAGGPA ASAGIQPGDV ILAVNGRPVT NPEQLRDAVK GAGNSLALLI QRDNAQIFVP VDLS

NT Sequence

ATGAACACCC GATTCCTTGC GCGTGGCGCC GTTGCGGTCG CCGTCGCTGC TGCGCTGTCC GCCGGCTACG TCGCGGGCAC CCGCCATGCC GATCCGCAGA TCATCACGCC CGCGCAGGCC GCCGCGCTGA TGCCGGCCGA GGCCGCGGCC AAGACCGGCA TTCCCGATTT CTCCGGGCTG GTCGAAACCT ACGGCCCGGC GGTCGTGAAC ATCAGCGCGA AGCACGTGGT CAAGCAGGTG TCGCGCCGCG TGCAGCAGCC GCAGCTGCCG ATGGATCCGA GCGACCCGTT CTACCAGTTC TTCAAGCACT TCTACGGCCA GGTGCCGGGC ATGGGCGGCG ACGCGCAGCC GGACGACCAG CCGAGCGCGA GCCTCGGCTC CGGGTTCATC GTCAGCCCCG ACGGCTACAT CCTCACCAAC GCGCACGTGA TCGACGGCGC GAACGTCGTC ACCGTCAAGC TGACCGACAA GCGCGAGTAC AAGGCGAAGG TCGTCGGCTC CGACAAGCAG TCCGACGTCG CGGTGCTGAA GATCGACGCG AGCGGCCTGC CGACCGTGAA GATCGGCGAC CCGGCGCGCA GCAAGGTCGG CCAGTGGGTC GTCGCGATCG GTTCGCCGTA CGGCTTCGAC AACACGGTCA CGTCGGGCAT CATCAGCGCG AAGTCGCGCG CGCTGCCGGA CGAGAACTAC ACGCCGTTCA TCCAGACCGA CGTGCCGGTC AACCCCGGCA ACTCGGGCGG CCCGCTGTTC AACCTGCAGG GTGAAGTGAT CGGCATCAAC TCGATGATCT ATTCGCAGAC GGGCGGCTTC CAGGGCCTGT CGTTCGCGAT CCCGATCAAC GAGGCGATCA AGGTCAAGGA CGAACTGGTG AAGACGGGCC ACGTGAGCCG CGGCCGCCTC GGCGTCGCCG TGCAGGGGCT GAACCAGACG CTCGCGAGCT CGTTCGGGCT GCCGAAGCCG GACGGCGCGC TGGTCAGCTC GGTCGATCCG AGCGGCCCGG CCGCGAAGGC CGGCCTGCAA CCGGGCGACG TGATCCTCGC GGTGAACGGT TCGCCGGTGG CCGATTCGAC GTCGCTGCCC GCGCAGATCG CGAGCCTGAA GCCCGGCTCG CAGGCCGACC TGCAGATCTG GCGCGACAAG TCGAAGAAGT CGATCAGCGT GACGCTCGGC GCGATGACCG ACGCGAAGCT GGCGTCGAAC GACGGCGGCC CCGTCGAGCA GGGGCGCCTC GGCGTCGCGG TGCGGCCGCT GACGCCGCAG GAGCGCAGCG CGACGAACCT GTCGCACGGG CTGATGGTGC AGCAGGCGGG CGGCCCCGCG GCCAGCGCGG GCATCCAGCC GGGCGACGTG ATCCTCGCGG TGAACGGCCG GCCGGTCACG AACCCCGAGC AATTGCGCGA CGCCGTCAAG GGAGCGGGCA ACAGTCTTGC TCTGCTGATC CAGCGCGATA ATGCACAGAT CTTCGTGCCG GTCGATCTGA GC

Details for BuviA.10100.b.B2.GE39321

HARVESTED ON:	7/10/2015
SEQUENCED ON:	7/6/2015
EXPECTED MW:	49kDa
OBSERVED MW:	49kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY:	data unavailable
PERCENT COVERAGE:	data unavailable

Validated AA Sequence

sequence unavailable

Validated NT Sequence

sequence unavailable

Expected Protein Sequence

MAHHHHHHAD PQIITPAQAA ALMPAEAAAK TGIPDFSGLV ETYGPAVVNI SAKHVVKQVS RRVQQPQLPM DPSDPFYQFF KHFYGQVPGM GGDAQPDDQP SASLGSGFIV SPDGYILTNA HVIDGANVVT VKLTDKREYK AKVVGSDKQS DVAVLKIDAS GLPTVKIGDP ARSKVGQWVV AIGSPYGFDN TVTSGIISAK SRALPDENYT PFIQTDVPVN PGNSGGPLFN LQGEVIGINS MIYSQTGGFQ GLSFAIPINE AIKVKDELVK TGHVSRGRLG VAVQGLNQTL ASSFGLPKPD GALVSSVDPS GPAAKAGLQP GDVILAVNGS PVADSTSLPA QIASLKPGSQ ADLQIWRDKS KKSISVTLGA MTDAKLASND GGPVEQGRLG VAVRPLTPQE RSATNLSHGL MVQQAGGPAA SAGIQPGDVI LAVNGRPVTN PEQLRDAVKG AGNSLALLIQ RDNAQIFVPV DLS

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgccgatc cgcagatcat cacgcccgcg caggccgccg cgctgatgcc ggccgaggcc gcggccaaga ccggcattcc cgatttctcc gggctggtcg aaacctacgg cccggcggtc gtgaacatca gcgcgaagca cgtggtcaag caggtgtcgc gccgcgtgca gcagccgcag ctgccgatgg atccgagcga cccgttctac cagttcttca agcacttcta cggccaggtg ccgggcatgg gcggcgacgc gcagccggac gaccagccga gcgcgagcct cggctccggg ttcatcgtca gccccgacgg ctacatcctc accaacgcgc acgtgatcga cggcgcgaac gtcgtcaccg tcaagctgac cgacaagcgc gagtacaagg cgaaggtcgt cggctccgac aagcagtccg acgtcgcggt gctgaagatc gacgcgagcg gcctgccgac cgtgaagatc ggcgacccgg cgcgcagcaa ggtcggccag tgggtcgtcg cgatcggttc gccgtacggc ttcgacaaca cggtcacgtc gggcatcatc agcgcgaagt cgcgcgcgct gccggacgag aactacacgc cgttcatcca gaccgacgtg ccggtcaacc ccggcaactc gggcggcccg ctgttcaacc tgcagggtga agtgatcggc atcaactcga tgatctattc gcagacgggc ggcttccagg gcctgtcgtt cgcgatcccg atcaacgagg cgatcaaggt caaggacgaa ctggtgaaga cgggccacgt gagccgcggc cgcctcggcg tcgccgtgca ggggctgaac cagacgctcg cgagctcgtt cgggctgccg aagccggacg gcgcgctggt cagctcggtc gatccgagcg gcccggccgc gaaggccggc ctgcaaccgg gcgacgtgat cctcgcggtg aacggttcgc cggtggccga ttcgacgtcg ctgcccgcgc agatcgcgag cctgaagccc ggctcgcagg ccgacctgca gatctggcgc gacaagtcga agaagtcgat cagcgtgacg ctcggcgcga tgaccgacgc gaagctggcg tcgaacgacg gcggccccgt cgagcagggg cgcctcggcg tcgcggtgcg gccgctgacg ccgcaggagc gcagcgcgac gaacctgtcg cacgggctga tggtgcagca ggcgggcggc cccgcggcca gcgcgggcat ccagccgggc gacgtgatcc tcgcggtgaa cggccggccg gtcacgaacc ccgagcaatt gcgcgacgcc gtcaagggag cgggcaacag tcttgctctg ctgatccagc gcgataatgc acagatcttc gtgccggtcg atctgagctg agtaagatag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaattcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgcagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gctgcggtaa agctcatcag cgtggtcgtg aagcgattca cagatgtctg cctgttcatc cgcgtccagc tcgttgagtt tctccagaag cgttaatgtc tggcttctga taaagcgggc catgttaagg gcggtttttt cctgtttggt cactgatgcc tccgtgtaag ggggatttct gttcatgggg gtaatgatac cgatgaaacg agagaggatg ctcacgatac gggttactga tgatgaacat gcccggttac tggaacgttg tgagggtaaa caactggcgg tatggatgcg gcgggaccag agaaaaatca ctcagggtca atgccagcgc ttcgttaata cagatgtagg tgttccacag ggtagccagc agcatcctgc gatgcagatc cggaacataa tggtgcaggg cgctgacttc cgcgtttcca gactttacga aacacggaaa ccgaagacca ttcatgttgt tgctcaggtc gcagacgttt tgcagcagca gtcgcttcac gttcgctcgc gtatcggtga ttcattctgc taaccagtaa ggcaaccccg ccagcctagc cgggtcctca acgacaggag cacgatcatg cgcacccgtg gccaggaccc aacgctgccc gagatgcgcc gcgtgcggct gctggagatg gcggacgcga tggatatgtt ctgccaaggg ttggtttgcg cattcacagt tctccgcaag aattgattgg ctccaattct tggagtggtg aatccgttag cgaggtgccg ccggcttcca ttcaggtcga ggtggcccgg ctccatgcac cgcgacgcaa cgcggggagg cagacaaggt atagggcggc gcctacaatc catgccaacc cgttccatgt gctcgccgag gcggcataaa tcgccgtgac gatcagcggt ccagtgatcg aagttaggct ggtaagagcc gcgagcgatc cttgaagctg tccctgatgg tcgtcatcta cctgcctgga cagcatggcc tgcaacgcgg gcatcccgat gccgccggaa gcgagaagaa tcataatggg gaaggccatc cagcctcgcg tcgcgaacgc cagcaagacg tagcccagcg cgtcggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga aat

Details for BuviA.10100.b.B2.PS02532

PURIFICATION DATe:	11/5/2015
CONCENTRATION:	22.26mg/ml
OBSERVED MW:	50kDa
EXPRESSION LEVEL:	Low Expression
PROTEIN PURIFICATION BUFFER:	SEC buffer
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	1
VIAL VOLUME:	110µl
PERCENT IDENTITY:	data unavailable
PERCENT COVERAGE:	data unavailable

Protocol Notes

notes unavailable

Validated AA Sequence

sequence unavailable

Validated NT Sequence

sequence unavailable

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATGCCGATC CGCAGATCAT CACGCCCGCG CAGGCCGCCG CGCTGATGCC GGCCGAGGCC GCGGCCAAGA CCGGCATTCC CGATTTCTCC GGGCTGGTCG AAACCTACGG CCCGGCGGTC GTGAACATCA GCGCGAAGCA CGTGGTCAAG CAGGTGTCGC GCCGCGTGCA GCAGCCGCAG CTGCCGATGG ATCCGAGCGA CCCGTTCTAC CAGTTCTTCA AGCACTTCTA CGGCCAGGTG CCGGGCATGG GCGGCGACGC GCAGCCGGAC GACCAGCCGA GCGCGAGCCT CGGCTCCGGG TTCATCGTCA GCCCCGACGG CTACATCCTC ACCAACGCGC ACGTGATCGA CGGCGCGAAC GTCGTCACCG TCAAGCTGAC CGACAAGCGC GAGTACAAGG CGAAGGTCGT CGGCTCCGAC AAGCAGTCCG ACGTCGCGGT GCTGAAGATC GACGCGAGCG GCCTGCCGAC CGTGAAGATC GGCGACCCGG CGCGCAGCAA GGTCGGCCAG TGGGTCGTCG CGATCGGTTC GCCGTACGGC TTCGACAACA CGGTCACGTC GGGCATCATC AGCGCGAAGT CGCGCGCGCT GCCGGACGAG AACTACACGC CGTTCATCCA GACCGACGTG CCGGTCAACC CCGGCAACTC GGGCGGCCCG CTGTTCAACC TGCAGGGTGA AGTGATCGGC ATCAACTCGA TGATCTATTC GCAGACGGGC GGCTTCCAGG GCCTGTCGTT CGCGATCCCG ATCAACGAGG CGATCAAGGT CAAGGACGAA CTGGTGAAGA CGGGCCACGT GAGCCGCGGC CGCCTCGGCG TCGCCGTGCA GGGGCTGAAC CAGACGCTCG CGAGCTCGTT CGGGCTGCCG AAGCCGGACG GCGCGCTGGT CAGCTCGGTC GATCCGAGCG GCCCGGCCGC GAAGGCCGGC CTGCAACCGG GCGACGTGAT CCTCGCGGTG AACGGTTCGC CGGTGGCCGA TTCGACGTCG CTGCCCGCGC AGATCGCGAG CCTGAAGCCC GGCTCGCAGG CCGACCTGCA GATCTGGCGC GACAAGTCGA AGAAGTCGAT CAGCGTGACG CTCGGCGCGA TGACCGACGC GAAGCTGGCG TCGAACGACG GCGGCCCCGT CGAGCAGGGG CGCCTCGGCG TCGCGGTGCG GCCGCTGACG CCGCAGGAGC GCAGCGCGAC GAACCTGTCG CACGGGCTGA TGGTGCAGCA GGCGGGCGGC CCCGCGGCCA GCGCGGGCAT CCAGCCGGGC GACGTGATCC TCGCGGTGAA CGGCCGGCCG GTCACGAACC CCGAGCAATT GCGCGACGCC GTCAAGGGAG CGGGCAACAG TCTTGCTCTG CTGATCCAGC GCGATAATGC ACAGATCTTC GTGCCGGTCG ATCTGAGCTG AGTAAGATAG GATCCGGCTG CTAACAAAGC CCGAAAGGAA GCTGAGTTGG CTGCTGCCAC CGCTGAGCAA TAACTAGCAT AACCCCTTGG GGCCTCTAAA CGGGTCTTGA GGGGTTTTTT GCTGAAAGGA GGAACTATAT CCGGATATCC ACAGGACGGG TGTGGTCGCC ATGATCGCGT AGTCGATAGT GGCTCCAAGT AGCGAAGCGA GCAGGACTGG GCGGCGGCCA AAGCGGTCGG ACAGTGCTCC GAGAACGGGT GCGCATAGAA ATTGCATCAA CGCATATAGC GCTAGCAGCA CGCCATAGTG ACTGGCGATG CTGTCGGAAT GGACGATATC CCGCAAGAGG CCCGGCAGTA CCGGCATAAC CAAGCCTATG CCTACAGCAT CCAGGGTGAC GGTGCCGAGG ATGACGATGA GCGCATTGTT AGATTTCATA CACGGTGCCT GACTGCGTTA GCAATTTAAC TGTGATAAAC TACCGCATTA AAGCTTATCG ATGATAAGCT GTCAAACATG AGAATTCTTG AAGACGAAAG GGCCTCGTGA TACGCCTATT TTTATAGGTT AATGTCATGA TAATAATGGT TTCTTAGACG TCAGGTGGCA CTTTTCGGGG AAATGTGCGC GGAACCCCTA TTTGTTTATT TTTCTAAATA CATTCAAATA TGTATCCGCT CATGAGACAA TAACCCTGAT AAATGCTTCA ATAATATTGA AAAAGGAAGA GTATGAGTAT TCAACATTTC CGTGTCGCCC TTATTCCCTT TTTTGCGGCA TTTTGCCTTC CTGTTTTTGC TCACCCAGAA ACGCTGGTGA AAGTAAAAGA TGCTGAAGAT CAGTTGGGTG CACGAGTGGG TTACATCGAA CTGGATCTCA ACAGCGGTAA GATCCTTGAG AGTTTTCGCC CCGAAGAACG TTTTCCAATG ATGAGCACTT TTAAAGTTCT GCTATGTGGC GCGGTATTAT CCCGTGTTGA CGCCGGGCAA GAGCAACTCG GTCGCCGCAT ACACTATTCT CAGAATGACT TGGTTGAGTA CTCACCAGTC ACAGAAAAGC ATCTTACGGA TGGCATGACA GTAAGAGAAT TATGCAGTGC TGCCATAACC ATGAGTGATA ACACTGCGGC CAACTTACTT CTGACAACGA TCGGAGGACC GAAGGAGCTA ACCGCTTTTT TGCACAACAT GGGGGATCAT GTAACTCGCC TTGATCGTTG GGAACCGGAG CTGAATGAAG CCATACCAAA CGACGAGCGT GACACCACGA TGCCTGCAGC AATGGCAACA ACGTTGCGCA AACTATTAAC TGGCGAACTA CTTACTCTAG CTTCCCGGCA ACAATTAATA GACTGGATGG AGGCGGATAA AGTTGCAGGA CCACTTCTGC GCTCGGCCCT TCCGGCTGGC TGGTTTATTG CTGATAAATC TGGAGCCGGT GAGCGTGGGT CTCGCGGTAT CATTGCAGCA CTGGGGCCAG ATGGTAAGCC CTCCCGTATC GTAGTTATCT ACACGACGGG GAGTCAGGCA ACTATGGATG AACGAAATAG ACAGATCGCT GAGATAGGTG CCTCACTGAT TAAGCATTGG TAACTGTCAG ACCAAGTTTA CTCATATATA CTTTAGATTG ATTTAAAACT TCATTTTTAA TTTAAAAGGA TCTAGGTGAA GATCCTTTTT GATAATCTCA TGACCAAAAT CCCTTAACGT GAGTTTTCGT TCCACTGAGC GTCAGACCCC GTAGAAAAGA TCAAAGGATC TTCTTGAGAT CCTTTTTTTC TGCGCGTAAT CTGCTGCTTG CAAACAAAAA AACCACCGCT ACCAGCGGTG GTTTGTTTGC CGGATCAAGA GCTACCAACT CTTTTTCCGA AGGTAACTGG CTTCAGCAGA GCGCAGATAC CAAATACTGT CCTTCTAGTG TAGCCGTAGT TAGGCCACCA CTTCAAGAAC TCTGTAGCAC CGCCTACATA CCTCGCTCTG CTAATCCTGT TACCAGTGGC TGCTGCCAGT GGCGATAAGT CGTGTCTTAC CGGGTTGGAC TCAAGACGAT AGTTACCGGA TAAGGCGCAG CGGTCGGGCT GAACGGGGGG TTCGTGCACA CAGCCCAGCT TGGAGCGAAC GACCTACACC GAACTGAGAT ACCTACAGCG TGAGCTATGA GAAAGCGCCA CGCTTCCCGA AGGGAGAAAG GCGGACAGGT ATCCGGTAAG CGGCAGGGTC GGAACAGGAG AGCGCACGAG GGAGCTTCCA GGGGGAAACG CCTGGTATCT TTATAGTCCT GTCGGGTTTC GCCACCTCTG ACTTGAGCGT CGATTTTTGT GATGCTCGTC AGGGGGGCGG AGCCTATGGA AAAACGCCAG CAACGCGGCC TTTTTACGGT TCCTGGCCTT TTGCTGGCCT TTTGCTCACA TGTTCTTTCC TGCGTTATCC CCTGATTCTG TGGATAACCG TATTACCGCC TTTGAGTGAG CTGATACCGC TCGCCGCAGC CGAACGACCG AGCGCAGCGA GTCAGTGAGC GAGGAAGCGG AAGAGCGCCT GATGCGGTAT TTTCTCCTTA CGCATCTGTG CGGTATTTCA CACCGCATAT ATGGTGCACT CTCAGTACAA TCTGCTCTGA TGCCGCATAG TTAAGCCAGT ATACACTCCG CTATCGCTAC GTGACTGGGT CATGGCTGCG CCCCGACACC CGCCAACACC CGCTGACGCG CCCTGACGGG CTTGTCTGCT CCCGGCATCC GCTTACAGAC AAGCTGTGAC CGTCTCCGGG AGCTGCATGT GTCAGAGGTT TTCACCGTCA TCACCGAAAC GCGCGAGGCA GCTGCGGTAA AGCTCATCAG CGTGGTCGTG AAGCGATTCA CAGATGTCTG CCTGTTCATC CGCGTCCAGC TCGTTGAGTT TCTCCAGAAG CGTTAATGTC TGGCTTCTGA TAAAGCGGGC CATGTTAAGG GCGGTTTTTT CCTGTTTGGT CACTGATGCC TCCGTGTAAG GGGGATTTCT GTTCATGGGG GTAATGATAC CGATGAAACG AGAGAGGATG CTCACGATAC GGGTTACTGA TGATGAACAT GCCCGGTTAC TGGAACGTTG TGAGGGTAAA CAACTGGCGG TATGGATGCG GCGGGACCAG AGAAAAATCA CTCAGGGTCA ATGCCAGCGC TTCGTTAATA CAGATGTAGG TGTTCCACAG GGTAGCCAGC AGCATCCTGC GATGCAGATC CGGAACATAA TGGTGCAGGG CGCTGACTTC CGCGTTTCCA GACTTTACGA AACACGGAAA CCGAAGACCA TTCATGTTGT TGCTCAGGTC GCAGACGTTT TGCAGCAGCA GTCGCTTCAC GTTCGCTCGC GTATCGGTGA TTCATTCTGC TAACCAGTAA GGCAACCCCG CCAGCCTAGC CGGGTCCTCA ACGACAGGAG CACGATCATG CGCACCCGTG GCCAGGACCC AACGCTGCCC GAGATGCGCC GCGTGCGGCT GCTGGAGATG GCGGACGCGA TGGATATGTT CTGCCAAGGG TTGGTTTGCG CATTCACAGT TCTCCGCAAG AATTGATTGG CTCCAATTCT TGGAGTGGTG AATCCGTTAG CGAGGTGCCG CCGGCTTCCA TTCAGGTCGA GGTGGCCCGG CTCCATGCAC CGCGACGCAA CGCGGGGAGG CAGACAAGGT ATAGGGCGGC GCCTACAATC CATGCCAACC CGTTCCATGT GCTCGCCGAG GCGGCATAAA TCGCCGTGAC GATCAGCGGT CCAGTGATCG AAGTTAGGCT GGTAAGAGCC GCGAGCGATC CTTGAAGCTG TCCCTGATGG TCGTCATCTA CCTGCCTGGA CAGCATGGCC TGCAACGCGG GCATCCCGAT GCCGCCGGAA GCGAGAAGAA TCATAATGGG GAAGGCCATC CAGCCTCGCG TCGCGAACGC CAGCAAGACG TAGCCCAGCG CGTCGGCCGC CATGCCGGCG ATAATGGCCT GCTTCTCGCC GAAACGTTTG GTGGCGGGAC CAGTGACGAA GGCTTGAGCG AGGGCGTGCA AGATTCCGAA TACCGCAAGC GACAGGCCGA TCATCGTCGC GCTCCAGCGA AAGCGGTCCT CGCCGAAAAT GACCCAGAGC GCTGCCGGCA CCTGTCCTAC GAGTTGCATG ATAAAGAAGA CAGTCATAAG TGCGGCGACG ATAGTCATGC CCCGCGCCCA CCGGAAGGAG CTGACTGGGT TGAAGGCTCT CAAGGGCATC GGTCGACGCT CTCCCTTATG CGACTCCTGC ATTAGGAAGC AGCCCAGTAG TAGGTTGAGG CCGTTGAGCA CCGCCGCCGC AAGGAATGGT GCATGCAAGG AGATGGCGCC CAACAGTCCC CCGGCCACGG GGCCTGCCAC CATACCCACG CCGAAACAAG CGCTCATGAG CCCGAAGTGG CGAGCCCGAT CTTCCCCATC GGTGATGTCG GCGATATAGG CGCCAGCAAC CGCACCTGTG GCGCCGGTGA TGCCGGCCAC GATGCGTCCG GCGTAGAGGA TCGAGATCTC GATCCCGCGA AAT