SSGCID: Target Page

BuviA.01673.o

RND efflux system, outer membrane lipoprotein, NodT family

CENTER ID:	BuviA.01673.o
ORGANISM:	Burkholderia vietnamiensis G4
ASSOCIATED DISEASE:
CURRENT STATUS:	purified
COMMUNITY REQUEST:	True
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BuviA.01673.o.A2.GE32450	Mature protein( BuviA.01673.o )		29	477

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BuviA.01673.o.A2.PW32370	Mature protein( BuviA.01673.o )		29	477

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|269482.11.peg.1736
RefSeq:	YP_001115529.1
UniProt:	A4JSM1

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MKRASALLAA SLLSACSIAP QPLAPVALGH DGFQHADASA EHAPSMQAWP AWFGDPQLHS LVDAALARNF DVRAAAARVA QAQALLGMRD AALWPSVRVD PSFSHARVSG TVDNALPKRI MHNWSVPVAA SYEVDLWGRL RGDADVGAQN VLQAAADRDA VRLRVATEVA VDYLTLRYVD DDLATLDSAI GLRRTALDVI AARVRAGAAS DLDALRASAD LDTARADLAD SRRLRENLVD AIAVLTGMAP SAFELEASAV PVRVPDVPPG LPSTLLARRP DVFAAARRVD AASLEVGIAR TAWLPTLTLT ADGGFATRDL RTFIDRNSSL WSLGANVALT LFDGGKRDAA VAAARAGVEV ADANYRATAI GALRDVQDAL NDIAAQRERI ARYGDAVRAT DAAARLSLSR YAHGYVGYLE VIDADRDALN ARRQLIHSRQ ALAIATVSLV RALGGGWTAP AGAGRQAARD AVQTDAQ

NT Sequence

ATGAAGCGCG CATCCGCCCT GCTCGCCGCG AGCCTGTTGA GCGCGTGCTC GATCGCACCG CAGCCGCTCG CGCCGGTGGC GCTCGGGCAC GACGGGTTTC AGCATGCCGA CGCGTCGGCC GAGCATGCGC CATCGATGCA AGCATGGCCC GCATGGTTCG GCGACCCGCA ACTGCACTCG CTCGTCGACG CCGCGCTCGC GCGCAACTTC GACGTGCGCG CCGCCGCCGC GCGCGTCGCG CAGGCGCAAG CGCTGCTCGG CATGCGCGAC GCCGCGCTGT GGCCGAGCGT GCGCGTCGAT CCGTCGTTCA GCCACGCGCG CGTATCAGGC ACCGTCGACA ACGCGTTGCC GAAGCGCATC ATGCACAACT GGTCGGTGCC CGTCGCCGCG AGTTACGAAG TCGATCTGTG GGGCCGGCTG CGCGGCGACG CGGATGTCGG TGCGCAGAAT GTGCTGCAAG CCGCGGCCGA TCGCGATGCG GTTCGGCTGC GGGTGGCAAC CGAGGTTGCC GTCGACTACC TGACGCTGCG CTACGTCGAC GACGATCTCG CGACGCTCGA CAGCGCAATC GGGCTGCGCC GCACCGCGCT GGACGTGATC GCCGCCCGCG TGCGGGCCGG CGCGGCGAGC GACCTCGACG CACTGCGCGC GTCGGCCGAT CTCGATACCG CGCGCGCCGA TCTCGCGGAC AGCCGGCGCT TGCGCGAGAA TCTCGTCGAT GCCATTGCGG TGCTGACGGG TATGGCCCCG AGCGCGTTCG AGCTCGAGGC GAGCGCCGTG CCCGTTCGCG TGCCGGACGT GCCGCCCGGC CTGCCGTCGA CGCTGCTCGC GCGCCGTCCG GACGTGTTCG CGGCCGCGCG GCGTGTCGAT GCCGCGTCGC TCGAGGTCGG CATCGCGCGC ACCGCGTGGC TGCCGACGCT GACGCTGACC GCCGACGGCG GCTTCGCGAC CCGCGACCTG CGCACCTTCA TCGATCGCAA CAGCTCGCTG TGGAGCCTCG GCGCGAACGT CGCGCTGACG CTGTTCGACG GTGGCAAACG CGATGCCGCG GTGGCGGCCG CGCGAGCCGG CGTCGAGGTT GCCGACGCGA ACTACCGGGC GACTGCGATC GGCGCGTTGC GCGACGTACA GGACGCGCTC AATGACATCG CCGCGCAACG GGAGCGGATC GCGCGCTACG GCGACGCCGT GCGCGCGACG GATGCCGCCG CGCGGCTGTC GCTGAGCCGC TATGCGCATG GTTACGTCGG CTATCTGGAA GTCATCGATG CGGACCGTGA CGCGTTGAAT GCCCGGCGCC AGTTGATTCA CAGCCGGCAA GCGCTCGCGA TCGCGACGGT GAGCCTCGTG CGTGCGCTGG GTGGCGGCTG GACGGCGCCC GCGGGAGCGG GCCGGCAAGC GGCGCGCGAT GCGGTGCAAA CCGATGCGCA G

Details for BuviA.01673.o.A2.GE32450

HARVESTED ON:	11/8/2011
SEQUENCED ON:	11/11/2011
EXPECTED MW:	50kDa
OBSERVED MW:	50kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	High Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	100
PERCENT COVERAGE:	94

Validated AA Sequence

MGHDGFQHAD ASAEHAPSMQ AWPAWFGDPQ LHSLVDAALA RNFDVRAAAA RVAQAQALLG MRDAALWPSV RVDPSFSHAR VSGTVDNALP KRIMHNWSVP VAASYEVDLW GRLRGDADVG AQNVLQAAAD RDAVRLRVAT EVAVDYLTLR YVDDDLATLD SAIGLRRTAL DVIAARVRAG AASDLDALRA SADLDTARAD LADSRRLREN LVDAIAVLTG MAPSAFELEA SAVPVRVPDV PPGLPSTLLA RRPDVFAAAR RVDAASLEVG IARTAWLPTL TLTADGGFAT RDLRTFIDRN SSLWSLGANV ALTLFDGGKR DAAVAAARAG VEVADANYRA TAIGALRDVQ DALNDIAAQR ERIARYGDAV RATDAAARLS LSRYAHGYVG YLEVIDADRD ALNARRQLIH SRQALAIATV SLVRALGGGW TAPAGAGRQA ARD

Validated NT Sequence

gcatcgcgcg ccgcttgccg gcccgctccc gcgggcgccg tccagccgcc acccagcgca cgcacgaggc tcaccgtcgc gatcgcgagc gcttgccggc tgtgaatcaa ctggcgccgg gcattcaacg cgtcacggtc cgcatcgatg acttccagat agccgacgta accatgcgca tagcggctca gcgacagccg cgcggcggca tccgtcgcgc gcacggcgtc gccgtagcgc gcgatccgct cccgttgcgc ggcgatgtca ttgagcgcgt cctgtacgtc gcgcaacgcg ccgatcgcag tcgcccggta gttcgcgtcg gcaacctcga cgccggctcg cgcggccgcc accgcggcat cgcgtttgcc accgtcgaac agcgtcagcg cgacgttcgc gccgaggctc cacagcgagc tgttgcgatc gatgaaggtg cgcaggtcgc gggtcgcgaa gccgccgtcg gcggtcagcg tcagcgtcgg cagccacgcg gtgcgcgcga tgccgacctc gagcgacgcg gcatcgacac gccgcgcggc cgcgaacacg tccggacggc gcgcgagcag cgtcgacggc aggccgggcg gcacgtccgg cacgcgaacg ggcacggcgc tcgcctcgag ctcgaacgcg ctcggggcca tacccgtcag caccgcaatg gcatcgacga gattctcgcg caagcgccgg ctgtccgcga gatcggcgcg cgcggtatcg agatcggccg acgcgcgcag tgcgtcgagg tcgctcgccg cgccggcccg cacgcgggcg gcgatcacgt ccagcgcggt gcggcgcagc ccgattgcgc tgtcgagcgt cgcgagatcg tcgtcgacgt agcgcagcgt caggtagtcg acggcaacct cggttgccac ccgcagccga accgcatcgc gatcggccgc ggcttgcagc acattctgcg caccgacatc cgcgtcgccg cgcagccggc cccacagatc gacttcgtaa ctcgcggcga cgggcaccga ccagttgtgc atgatgcgct tcggcaacgc gttgtcgacg gtgcctgata cgcgcgcgtg gctgaacgac ggatcgacgc gcacgctcgg ccacagcgcg gcgtcgcgca tgccgagcag cgcttgcgcc tgcgcgacgc gcgcggcggc ggcgcgcacg tcgaagttgc gcgcgagcgc ggcgtcgacg agcgagtgca gttgcgggtc gccgaaccat gcgggccatg cttgcatcga tggcgcatgc tcggccgacg cgtcggcatg ctgaaacccg tcgtgcccca tcgaaccagg accctgggtc tgagcttcca gggtaccca

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMGHDGFQHA DASAEHAPSM QAWPAWFGDP QLHSLVDAAL ARNFDVRAAA ARVAQAQALL GMRDAALWPS VRVDPSFSHA RVSGTVDNAL PKRIMHNWSV PVAASYEVDL WGRLRGDADV GAQNVLQAAA DRDAVRLRVA TEVAVDYLTL RYVDDDLATL DSAIGLRRTA LDVIAARVRA GAASDLDALR ASADLDTARA DLADSRRLRE NLVDAIAVLT GMAPSAFELE ASAVPVRVPD VPPGLPSTLL ARRPDVFAAA RRVDAASLEV GIARTAWLPT LTLTADGGFA TRDLRTFIDR NSSLWSLGAN VALTLFDGGK RDAAVAAARA GVEVADANYR ATAIGALRDV QDALNDIAAQ RERIARYGDA VRATDAAARL SLSRYAHGYV GYLEVIDADR DALNARRQLI HSRQALAIAT VSLVRALGGG WTAPAGAGRQ AARDAVQTDA Q

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggggcacgac gggtttcagc atgccgacgc gtcggccgag catgcgccat cgatgcaagc atggcccgca tggttcggcg acccgcaact gcactcgctc gtcgacgccg cgctcgcgcg caacttcgac gtgcgcgccg ccgccgcgcg cgtcgcgcag gcgcaagcgc tgctcggcat gcgcgacgcc gcgctgtggc cgagcgtgcg cgtcgatccg tcgttcagcc acgcgcgcgt atcaggcacc gtcgacaacg cgttgccgaa gcgcatcatg cacaactggt cggtgcccgt cgccgcgagt tacgaagtcg atctgtgggg ccggctgcgc ggcgacgcgg atgtcggtgc gcagaatgtg ctgcaagccg cggccgatcg cgatgcggtt cggctgcggg tggcaaccga ggttgccgtc gactacctga cgctgcgcta cgtcgacgac gatctcgcga cgctcgacag cgcaatcggg ctgcgccgca ccgcgctgga cgtgatcgcc gcccgcgtgc gggccggcgc ggcgagcgac ctcgacgcac tgcgcgcgtc ggccgatctc gataccgcgc gcgccgatct cgcggacagc cggcgcttgc gcgagaatct cgtcgatgcc attgcggtgc tgacgggtat ggccccgagc gcgttcgagc tcgaggcgag cgccgtgccc gttcgcgtgc cggacgtgcc gcccggcctg ccgtcgacgc tgctcgcgcg ccgtccggac gtgttcgcgg ccgcgcggcg tgtcgatgcc gcgtcgctcg aggtcggcat cgcgcgcacc gcgtggctgc cgacgctgac gctgaccgcc gacggcggct tcgcgacccg cgacctgcgc accttcatcg atcgcaacag ctcgctgtgg agcctcggcg cgaacgtcgc gctgacgctg ttcgacggtg gcaaacgcga tgccgcggtg gcggccgcgc gagccggcgt cgaggttgcc gacgcgaact accgggcgac tgcgatcggc gcgttgcgcg acgtacagga cgcgctcaat gacatcgccg cgcaacggga gcggatcgcg cgctacggcg acgccgtgcg cgcgacggat gccgccgcgc ggctgtcgct gagccgctat gcgcatggtt acgtcggcta tctggaagtc atcgatgcgg accgtgacgc gttgaatgcc cggcgccagt tgattcacag ccggcaagcg ctcgcgatcg cgacggtgag cctcgtgcgt gcgctgggtg gcggctggac ggcgcccgcg ggagcgggcc ggcaagcggc gcgcgatgcg gtgcaaaccg atgcgcagta aacagcacga acaagttctg cagccaagct tctcgaggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga a

Details for BuviA.01673.o.A2.PW32370

PURIFICATION DATe:	3/3/2012
CONCENTRATION:	3.34mg/ml
OBSERVED MW:	data unavailable
EXPRESSION LEVEL:	Moderate Expression
PROTEIN PURIFICATION BUFFER:	25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	15
VIAL VOLUME:	200µl
PERCENT IDENTITY:	100
PERCENT COVERAGE:	94

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGGGCACGAC GGGTTTCAGC ATGCCGACGC GTCGGCCGAG CATGCGCCAT CGATGCAAGC ATGGCCCGCA TGGTTCGGCG ACCCGCAACT GCACTCGCTC GTCGACGCCG CGCTCGCGCG CAACTTCGAC GTGCGCGCCG CCGCCGCGCG CGTCGCGCAG GCGCAAGCGC TGCTCGGCAT GCGCGACGCC GCGCTGTGGC CGAGCGTGCG CGTCGATCCG TCGTTCAGCC ACGCGCGCGT ATCAGGCACC GTCGACAACG CGTTGCCGAA GCGCATCATG CACAACTGGT CGGTGCCCGT CGCCGCGAGT TACGAAGTCG ATCTGTGGGG CCGGCTGCGC GGCGACGCGG ATGTCGGTGC GCAGAATGTG CTGCAAGCCG CGGCCGATCG CGATGCGGTT CGGCTGCGGG TGGCAACCGA GGTTGCCGTC GACTACCTGA CGCTGCGCTA CGTCGACGAC GATCTCGCGA CGCTCGACAG CGCAATCGGG CTGCGCCGCA CCGCGCTGGA CGTGATCGCC GCCCGCGTGC GGGCCGGCGC GGCGAGCGAC CTCGACGCAC TGCGCGCGTC GGCCGATCTC GATACCGCGC GCGCCGATCT CGCGGACAGC CGGCGCTTGC GCGAGAATCT CGTCGATGCC ATTGCGGTGC TGACGGGTAT GGCCCCGAGC GCGTTCGAGC TCGAGGCGAG CGCCGTGCCC GTTCGCGTGC CGGACGTGCC GCCCGGCCTG CCGTCGACGC TGCTCGCGCG CCGTCCGGAC GTGTTCGCGG CCGCGCGGCG TGTCGATGCC GCGTCGCTCG AGGTCGGCAT CGCGCGCACC GCGTGGCTGC CGACGCTGAC GCTGACCGCC GACGGCGGCT TCGCGACCCG CGACCTGCGC ACCTTCATCG ATCGCAACAG CTCGCTGTGG AGCCTCGGCG CGAACGTCGC GCTGACGCTG TTCGACGGTG GCAAACGCGA TGCCGCGGTG GCGGCCGCGC GAGCCGGCGT CGAGGTTGCC GACGCGAACT ACCGGGCGAC TGCGATCGGC GCGTTGCGCG ACGTACAGGA CGCGCTCAAT GACATCGCCG CGCAACGGGA GCGGATCGCG CGCTACGGCG ACGCCGTGCG CGCGACGGAT GCCGCCGCGC GGCTGTCGCT GAGCCGCTAT GCGCATGGTT ACGTCGGCTA TCTGGAAGTC ATCGATGCGG ACCGTGACGC GTTGAATGCC CGGCGCCAGT TGATTCACAG CCGGCAAGCG CTCGCGATCG CGACGGTGAG CCTCGTGCGT GCGCTGGGTG GCGGCTGGAC GGCGCCCGCG GGAGCGGGCC GGCAAGCGGC GCGCGATGCG GTGCAAACCG ATGCGCAGTA AACAGCACGA ACAAGTTCTG CAGCCAAGCT TCTCGAGGAT CCGGCTGCTA ACAAAGCCCG AAAGGAAGCT GAGTTGGCTG CTGCCACCGC TGAGCAATAA CTAGCATAAC CCCTTGGGGC CTCTAAACGG GTCTTGAGGG GTTTTTTGCT GAAAGGAGGA ACTATATCCG GATATCCACA GGACGGGTGT GGTCGCCATG ATCGCGTAGT CGATAGTGGC TCCAAGTAGC GAAGCGAGCA GGACTGGGCG GCGGCCAAAG CGGTCGGACA GTGCTCCGAG AACGGGTGCG CATAGAAATT GCATCAACGC ATATAGCGCT AGCAGCACGC CATAGTGACT GGCGATGCTG TCGGAATGGA CGATATCCCG CAAGAGGCCC GGCAGTACCG GCATAACCAA GCCTATGCCT ACAGCATCCA GGGTGACGGT GCCGAGGATG ACGATGAGCG CATTGTTAGA TTTCATACAC GGTGCCTGAC TGCGTTAGCA ATTTAACTGT GATAAACTAC CGCATTAAAG CTTATCGATG ATAAGCTGTC AAACATGAGA A