SSGCID: Target Page

BuviA.01673.m

RND efflux system, outer membrane lipoprotein, NodT family

CENTER ID:	BuviA.01673.m
ORGANISM:	Burkholderia vietnamiensis G4
ASSOCIATED DISEASE:
CURRENT STATUS:	purified
COMMUNITY REQUEST:	True
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BuviA.01673.m.A2.GE32448	Mature protein( BuviA.01673.m )		32	486

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BuviA.01673.m.A2.PS01342	Mature protein( BuviA.01673.m )		32	486

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|269482.11.peg.1543
RefSeq:	YP_001115339.1
UniProt:	A4JS31

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MPDRAPGRAS RRREGAAVAA AIAAAMLAAC TSIVKVDVPP QRDVPAAFEQ VPAADGTQAD LAHWWSQWQD PALARHVEDA LQANTDVRVA QARVREARAL AAVAESARYP SLSARAGGVH GFGNLRDPSQ VPDGSPDLDS YAGGITAGWE VDIFGRRASD AQAAALLADA AQEKLRGTRV AIAGDVAQNY LEAQGLQRRL DLLDRSLVTL HALQRYTDAR FRAGQATRYD VARVNEQIVA REAERPALAS QIDVRRRRLA ILAGQPPQQA APLAAPGPFR VPAVPAGELP SAVLERRPDV RATAAMVRAQ AARLGSAKAD LLPRFYLTFV GLDGRVHLDG LPGLSGTGGL IGIGADLPIF NAGRIRSNIE ANDARLQAAL AQHDKTLLQT LEEVDSAYGV RHGFDQRSER LVATLEIARR NADEARRLYE GGRRTLQDVL DARIDALRRE DELVQSQTGE ALATVRLYLA LGGGWSSGDE ASPGKT

NT Sequence

GTGCCGGATC GCGCGCCGGG GCGTGCGTCG CGCCGGCGAG AGGGCGCCGC CGTGGCCGCC GCGATCGCGG CGGCGATGCT CGCCGCCTGC ACGTCGATCG TGAAGGTCGA CGTGCCGCCT CAGCGCGACG TTCCCGCCGC GTTCGAGCAA GTGCCGGCGG CGGACGGCAC GCAAGCCGAC CTCGCGCACT GGTGGTCGCA ATGGCAGGAT CCCGCGCTTG CCCGACATGT CGAGGACGCG CTGCAGGCGA ACACGGATGT GCGCGTCGCG CAGGCGCGCG TGCGCGAGGC GCGCGCGCTC GCGGCGGTCG CCGAATCGGC GCGCTATCCG TCGCTGTCCG CGCGGGCGGG CGGCGTCCAC GGCTTCGGCA ACCTGCGCGA TCCGTCGCAG GTGCCCGACG GTTCGCCCGA TCTCGACAGT TATGCGGGCG GCATCACGGC CGGGTGGGAA GTCGACATCT TCGGCCGCCG CGCGAGCGAC GCGCAGGCCG CCGCGCTGCT GGCCGACGCC GCACAGGAAA AGCTGCGCGG CACGCGCGTC GCGATCGCGG GCGACGTCGC GCAGAACTAC CTGGAAGCGC AAGGGCTGCA GCGCAGGCTC GATCTGCTCG ATCGCAGCCT CGTCACGCTG CATGCGCTGC AACGCTATAC CGACGCGCGG TTTCGTGCCG GGCAGGCCAC GCGCTACGAC GTGGCGCGCG TGAACGAGCA GATCGTCGCG CGCGAGGCCG AGCGTCCCGC GCTGGCGAGC CAGATCGACG TGCGCAGGCG GCGGCTCGCG ATCCTCGCCG GCCAGCCTCC GCAACAGGCC GCGCCGCTGG CCGCGCCGGG GCCGTTTCGG GTGCCGGCCG TGCCGGCGGG CGAGCTGCCG TCGGCGGTGC TGGAGCGCCG GCCCGACGTG CGCGCGACGG CGGCGATGGT GCGCGCGCAG GCCGCACGGC TCGGCAGCGC GAAAGCCGAC CTGCTGCCGC GCTTCTATCT GACGTTCGTC GGCCTCGACG GCCGCGTGCA TCTCGACGGC CTGCCGGGGC TGAGCGGCAC GGGCGGGTTG ATCGGCATCG GCGCCGACCT GCCGATCTTC AACGCGGGGC GCATCCGCTC GAACATCGAG GCCAACGATG CGCGCCTGCA GGCCGCGCTC GCGCAGCACG ACAAGACGCT GCTGCAGACG CTCGAGGAAG TCGACAGCGC GTATGGCGTG CGTCACGGCT TCGACCAGCG CAGCGAGCGG CTGGTGGCGA CGCTCGAGAT CGCGCGCCGC AACGCGGACG AGGCCCGGCG CCTTTACGAA GGCGGCCGGC GGACGTTGCA GGACGTGCTC GATGCGCGCA TCGACGCGCT GCGGCGCGAA GACGAACTGG TGCAGTCGCA GACCGGCGAG GCGCTCGCGA CGGTGCGGCT GTATCTCGCG CTCGGCGGCG GCTGGTCGAG CGGCGACGAG GCATCGCCCG GCAAGACG

Details for BuviA.01673.m.A2.GE32448

HARVESTED ON:	11/8/2011
SEQUENCED ON:	11/11/2011
EXPECTED MW:	51kDa
OBSERVED MW:	51kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	High Expression
SOLUBLE EXPRESSION LEVEL	High Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	79
PERCENT COVERAGE:	84

Validated AA Sequence

MAHHHHHHMG TLEAQTQGPG SMSIVKVDVP PQRDVPAAFE QVPAADGTQA DLAHWWSQWQ DPALARHVED ALQANTDVRV AQARVREARA LAAVAESARY PSLSARAGGV HGFGNLRDPS QVPDGSPDLD SYAGGITAGW EVDIFGRRAS DAQAAALLAD AAQEKLRGTR VAIAGDVAQN YLEAQGLQRR LDLLDRSLVT LHALQRYTDA RFRAGQATRY DVARVNEQIV AREAERPALA SQIDVRRRRL AILAGQPPQQ XAPLAAPGPF XVXAVPAGEL PSAVLERRPD VRATAAMVRA XAARXGSAKA DLLPRFYLTF VGXXXAXIXT AXRGXXARXG XSXSXXPXDX XXXXXSXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXX

Validated NT Sequence

attttgttta actttaagaa ggagatatac catggctcat caccatcacc atcatatggg taccctggaa gctcagaccc agggtcctgg ttcgatgtcg atcgtgaagg tcgacgtgcc gcctcagcgc gacgttcccg ccgcgttcga gcaagtgccg gcggcggacg gcacgcaagc cgacctcgcg cactggtggt cgcaatggca ggatcccgcg cttgcccgac atgtcgagga cgcgctgcag gcgaacacgg atgtgcgcgt cgcgcaggcg cgcgtgcgcg aggcgcgcgc gctcgcggcg gtcgccgaat cggcgcgcta tccgtcgctg tccgcgcggg cgggcggcgt ccacggcttc ggcaacctgc gcgatccgtc gcaggtgccc gacggttcgc ccgatctcga cagttatgcg ggcggcatca cggccgggtg ggaagtcgac atcttcggcc gccgcgcgag cgacgcgcag gccgccgcgc tgctggccga cgccgcacag gaaaagctgc gcggcacgcg cgtcgcgatc gcgggcgacg tcgcgcagaa ctacctggaa gcgcaagggc tgcagcgcag gctcgatctg ctcgatcgca gcctcgtcac gctgcatgcg ctgcaacgct ataccgacgc gcggtttcgt gccgggcagg ccacgcgcta cgacgtggcg cgcgtgaacg agcagatcgt cgcgcgcgag gccgagcgtc ccgcgctggc gagccagatc gacgtgcgca ggcggcggct cgcgatcctc gccggccagc ctccgcaaca ggncgcgccg ctggccgcgc cggggccgtt tcnggtgncg gccgtgccgg cgggcgagct gccgtcngcg gtgctggagc gccggcccga cgtgcgcgcg acggcggcga tggtgcgcgc gcnggccgca cggntcggca gcgcgaaagc cgacctgctg ccgcgcttct atctgacgtt cgtcggcntc gangncgcnn gcatcncgac ggcctgncgg ggntnnnngg cacgggnngg tngatcggna tcgncncnac ctnncgatcn tnnnnnnngg gnnncatccn ntcnancatn nnnnnnnnat gnnnnnnnnc nnnnnnnnnn acnnnannnn nnnncnnnng nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn gnnngnnnnn nnnnnnntnn nnnnntnnnn nnnnnnnnnn na

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMSIVKVDVP PQRDVPAAFE QVPAADGTQA DLAHWWSQWQ DPALARHVED ALQANTDVRV AQARVREARA LAAVAESARY PSLSARAGGV HGFGNLRDPS QVPDGSPDLD SYAGGITAGW EVDIFGRRAS DAQAAALLAD AAQEKLRGTR VAIAGDVAQN YLEAQGLQRR LDLLDRSLVT LHALQRYTDA RFRAGQATRY DVARVNEQIV AREAERPALA SQIDVRRRRL AILAGQPPQQ AAPLAAPGPF RVPAVPAGEL PSAVLERRPD VRATAAMVRA QAARLGSAKA DLLPRFYLTF VGLDGRVHLD GLPGLSGTGG LIGIGADLPI FNAGRIRSNI EANDARLQAA LAQHDKTLLQ TLEEVDSAYG VRHGFDQRSE RLVATLEIAR RNADEARRLY EGGRRTLQDV LDARIDALRR EDELVQSQTG EALATVRLYL ALGGGWSSGD EASPGKT

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gtcgatcgtg aaggtcgacg tgccgcctca gcgcgacgtt cccgccgcgt tcgagcaagt gccggcggcg gacggcacgc aagccgacct cgcgcactgg tggtcgcaat ggcaggatcc cgcgcttgcc cgacatgtcg aggacgcgct gcaggcgaac acggatgtgc gcgtcgcgca ggcgcgcgtg cgcgaggcgc gcgcgctcgc ggcggtcgcc gaatcggcgc gctatccgtc gctgtccgcg cgggcgggcg gcgtccacgg cttcggcaac ctgcgcgatc cgtcgcaggt gcccgacggt tcgcccgatc tcgacagtta tgcgggcggc atcacggccg ggtgggaagt cgacatcttc ggccgccgcg cgagcgacgc gcaggccgcc gcgctgctgg ccgacgccgc acaggaaaag ctgcgcggca cgcgcgtcgc gatcgcgggc gacgtcgcgc agaactacct ggaagcgcaa gggctgcagc gcaggctcga tctgctcgat cgcagcctcg tcacgctgca tgcgctgcaa cgctataccg acgcgcggtt tcgtgccggg caggccacgc gctacgacgt ggcgcgcgtg aacgagcaga tcgtcgcgcg cgaggccgag cgtcccgcgc tggcgagcca gatcgacgtg cgcaggcggc ggctcgcgat cctcgccggc cagcctccgc aacaggccgc gccgctggcc gcgccggggc cgtttcgggt gccggccgtg ccggcgggcg agctgccgtc ggcggtgctg gagcgccggc ccgacgtgcg cgcgacggcg gcgatggtgc gcgcgcaggc cgcacggctc ggcagcgcga aagccgacct gctgccgcgc ttctatctga cgttcgtcgg cctcgacggc cgcgtgcatc tcgacggcct gccggggctg agcggcacgg gcgggttgat cggcatcggc gccgacctgc cgatcttcaa cgcggggcgc atccgctcga acatcgaggc caacgatgcg cgcctgcagg ccgcgctcgc gcagcacgac aagacgctgc tgcagacgct cgaggaagtc gacagcgcgt atggcgtgcg tcacggcttc gaccagcgca gcgagcggct ggtggcgacg ctcgagatcg cgcgccgcaa cgcggacgag gcccggcgcc tttacgaagg cggccggcgg acgttgcagg acgtgctcga tgcgcgcatc gacgcgctgc ggcgcgaaga cgaactggtg cagtcgcaga ccggcgaggc gctcgcgacg gtgcggctgt atctcgcgct cggcggcggc tggtcgagcg gcgacgaggc atcgcccggc aagacgtaaa cagcacgaac aagttctgca gccaagcttc tcgaggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaa

Details for BuviA.01673.m.A2.PS01342

PURIFICATION DATe:	1/10/2012
CONCENTRATION:	15.75mg/ml
OBSERVED MW:	data unavailable
EXPRESSION LEVEL:	High Expression
PROTEIN PURIFICATION BUFFER:	20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	3
VIAL VOLUME:	110µl
PERCENT IDENTITY:	79
PERCENT COVERAGE:	84

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMSIVKVDVP PQRDVPAAFE QVPAADGTQA DLAHWWSQWQ DPALARHVED ALQANTDVRV AQARVREARA LAAVAESARY PSLSARAGGV HGFGNLRDPS QVPDGSPDLD SYAGGITAGW EVDIFGRRAS DAQAAALLAD AAQEKLRGTR VAIAGDVAQN YLEAQGLQRR LDLLDRSLVT LHALQRYTDA RFRAGQATRY DVARVNEQIV AREAERPALA SQIDVRRRRL AILAGQPPQQ AAPLAAPGPF RVPAVPAGEL PSAVLERRPD VRATAAMVRA QAARLGSAKA DLLPRFYLTF VGLDGRVHLD GLPGLSGTGG LIGIGADLPI FNAGRIRSNI EANDARLQAA LAQHDKTLLQ TLEEVDSAYG VRHGFDQRSE RLVATLEIAR RNADEARRLY EGGRRTLQDV LDARIDALRR EDELVQSQTG EALATVRLYL ALGGGWSSGD EASPGKT

Full NT Sequence (Expression Vector + Insert)

TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GTCGATCGTG AAGGTCGACG TGCCGCCTCA GCGCGACGTT CCCGCCGCGT TCGAGCAAGT GCCGGCGGCG GACGGCACGC AAGCCGACCT CGCGCACTGG TGGTCGCAAT GGCAGGATCC CGCGCTTGCC CGACATGTCG AGGACGCGCT GCAGGCGAAC ACGGATGTGC GCGTCGCGCA GGCGCGCGTG CGCGAGGCGC GCGCGCTCGC GGCGGTCGCC GAATCGGCGC GCTATCCGTC GCTGTCCGCG CGGGCGGGCG GCGTCCACGG CTTCGGCAAC CTGCGCGATC CGTCGCAGGT GCCCGACGGT TCGCCCGATC TCGACAGTTA TGCGGGCGGC ATCACGGCCG GGTGGGAAGT CGACATCTTC GGCCGCCGCG CGAGCGACGC GCAGGCCGCC GCGCTGCTGG CCGACGCCGC ACAGGAAAAG CTGCGCGGCA CGCGCGTCGC GATCGCGGGC GACGTCGCGC AGAACTACCT GGAAGCGCAA GGGCTGCAGC GCAGGCTCGA TCTGCTCGAT CGCAGCCTCG TCACGCTGCA TGCGCTGCAA CGCTATACCG ACGCGCGGTT TCGTGCCGGG CAGGCCACGC GCTACGACGT GGCGCGCGTG AACGAGCAGA TCGTCGCGCG CGAGGCCGAG CGTCCCGCGC TGGCGAGCCA GATCGACGTG CGCAGGCGGC GGCTCGCGAT CCTCGCCGGC CAGCCTCCGC AACAGGCCGC GCCGCTGGCC GCGCCGGGGC CGTTTCGGGT GCCGGCCGTG CCGGCGGGCG AGCTGCCGTC GGCGGTGCTG GAGCGCCGGC CCGACGTGCG CGCGACGGCG GCGATGGTGC GCGCGCAGGC CGCACGGCTC GGCAGCGCGA AAGCCGACCT GCTGCCGCGC TTCTATCTGA CGTTCGTCGG CCTCGACGGC CGCGTGCATC TCGACGGCCT GCCGGGGCTG AGCGGCACGG GCGGGTTGAT CGGCATCGGC GCCGACCTGC CGATCTTCAA CGCGGGGCGC ATCCGCTCGA ACATCGAGGC CAACGATGCG CGCCTGCAGG CCGCGCTCGC GCAGCACGAC AAGACGCTGC TGCAGACGCT CGAGGAAGTC GACAGCGCGT ATGGCGTGCG TCACGGCTTC GACCAGCGCA GCGAGCGGCT GGTGGCGACG CTCGAGATCG CGCGCCGCAA CGCGGACGAG GCCCGGCGCC TTTACGAAGG CGGCCGGCGG ACGTTGCAGG ACGTGCTCGA TGCGCGCATC GACGCGCTGC GGCGCGAAGA CGAACTGGTG CAGTCGCAGA CCGGCGAGGC GCTCGCGACG GTGCGGCTGT ATCTCGCGCT CGGCGGCGGC TGGTCGAGCG GCGACGAGGC ATCGCCCGGC AAGACGTAAA CAGCACGAAC AAGTTCTGCA GCCAAGCTTC TCGAGGATCC GGCTGCTAAC AAAGCCCGAA AGGAAGCTGA GTTGGCTGCT GCCACCGCTG AGCAATAACT AGCATAACCC CTTGGGGCCT CTAAACGGGT CTTGAGGGGT TTTTTGCTGA AAGGAGGAAC TATATCCGGA TATCCACAGG ACGGGTGTGG TCGCCATGAT CGCGTAGTCG ATAGTGGCTC CAAGTAGCGA AGCGAGCAGG ACTGGGCGGC GGCCAAAGCG GTCGGACAGT GCTCCGAGAA CGGGTGCGCA TAGAAATTGC ATCAACGCAT ATAGCGCTAG CAGCACGCCA TAGTGACTGG CGATGCTGTC GGAATGGACG ATATCCCGCA AGAGGCCCGG CAGTACCGGC ATAACCAAGC CTATGCCTAC AGCATCCAGG GTGACGGTGC CGAGGATGAC GATGAGCGCA TTGTTAGATT TCATACACGG TGCCTGACTG CGTTAGCAAT TTAACTGTGA TAAACTACCG CATTAAAGCT TATCGATGAT AAGCTGTCAA ACATGAGAA