BuviA.01673.j

RND efflux system, outer membrane lipoprotein, NodT family

CENTER ID:	BuviA.01673.j
ORGANISM:	Burkholderia vietnamiensis G4
ASSOCIATED DISEASE:
CURRENT STATUS:	cloned
COMMUNITY REQUEST:	True
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BuviA.01673.j.A2.GE32445	Mature protein( BuviA.01673.j )		19	475

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
OrthoMCL:	OG5_134493
PATRIC ID:	fig\|269482.11.peg.3482
RefSeq:	YP_001117180.1
UniProt:	A4JN62

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MKRTLIALLC AGALGACSLD PTYQRPAAPI DDTWSTAAAP AQPQTAQSTA AAPLGADLGW RDFFKDPRLQ KLIELALANN RDMRVAALNV AQYEAQYRIA RANLGPAIDA SGSVTREHMA GSTSAYSSSS ASVGLTSWEI DFFGRLRSLK RQALEQYLAT DAARTSTQIS LIATVATDYL QLLSDEASLQ IAQNTVAADQ HTYDLTVNMM KLGSASLQDV RQAENSLASA RSSLASYTRA VAQDRNDLVA EIGAPLPDDL PQGPSLMESE HTLADIGAGV PSDLLARRPD IVEAEHTLKA ANANIGAARA AFFPKIELTA SAGTTSSGLS NLFKAGTGAW AFAPSISVPI FDFGYNRASL DVAKVEKDID VANYEKAIQT AFKEVSNALA GRTTYVTQVA ADRDYVSSAQ QYYDLAQAHY RSGTDSFLTL LDAQRTLYTA QQQLVTDMLA KQSNLITLYK VLGGGWSETS AVAQR

NT Sequence

ATGAAACGCA CACTGATCGC CCTTCTTTGC GCGGGCGCAC TCGGCGCCTG CTCACTGGAC CCGACGTACC AGCGGCCCGC AGCGCCGATC GACGACACGT GGTCCACCGC GGCCGCGCCG GCGCAGCCGC AGACGGCGCA GAGCACCGCT GCGGCACCGC TGGGTGCCGA CCTCGGCTGG CGCGACTTCT TCAAGGACCC GCGCCTGCAA AAGCTGATCG AACTCGCGCT CGCGAACAAC CGCGATATGC GCGTGGCCGC GCTCAACGTG GCGCAGTACG AAGCGCAGTA CCGCATCGCG CGCGCCAACC TCGGGCCGGC CATCGACGCG TCGGGGTCCG TCACGCGCGA ACACATGGCC GGCTCCACGA GCGCATACAG TTCGAGCAGC GCGAGCGTGG GTCTCACGTC GTGGGAAATC GACTTCTTCG GCCGCCTGCG CAGCCTGAAG CGCCAGGCGC TGGAACAGTA CCTCGCCACG GATGCCGCGC GCACCAGCAC GCAGATCAGC CTCATCGCGA CCGTCGCCAC CGACTATCTG CAACTGCTCT CCGACGAGGC TTCGTTGCAG ATCGCGCAGA ACACCGTGGC CGCCGACCAG CACACCTACG ACCTCACGGT GAACATGATG AAGCTGGGCA GCGCGTCGCT GCAGGACGTG CGCCAGGCCG AAAACTCGCT GGCGAGCGCG CGCTCGAGCC TCGCCTCGTA CACGCGTGCG GTGGCGCAGG ACCGCAACGA CCTGGTGGCC GAAATCGGCG CGCCGCTGCC CGACGATCTG CCCCAGGGCC CCTCGCTGAT GGAAAGCGAG CACACCCTCG CGGACATCGG CGCGGGCGTG CCGTCGGATC TGCTCGCGCG CCGGCCCGAC ATCGTCGAAG CAGAGCACAC GCTCAAGGCG GCCAACGCCA ACATCGGCGC GGCGCGCGCC GCGTTCTTTC CGAAGATCGA GCTGACGGCT TCCGCGGGCA CCACGAGCAG CGGCCTCTCG AACCTCTTCA AGGCGGGCAC GGGCGCGTGG GCGTTTGCGC CGTCCATCTC GGTGCCCATC TTCGACTTCG GCTACAACCG CGCGTCGCTG GACGTGGCGA AAGTCGAAAA GGACATCGAC GTCGCCAACT ACGAAAAGGC CATTCAGACG GCGTTCAAGG AAGTGTCGAA TGCGCTGGCG GGACGCACGA CGTACGTCAC GCAGGTGGCC GCGGACCGCG ACTACGTGAG CTCGGCGCAG CAGTACTACG ACCTCGCGCA GGCACACTAC CGCAGCGGCA CCGACAGCTT CCTGACCCTG CTCGACGCGC AGCGCACGCT TTACACGGCG CAACAGCAGC TCGTCACCGA CATGCTCGCC AAGCAGTCGA ACCTGATCAC GCTGTACAAG GTACTGGGCG GCGGCTGGTC GGAGACGAGC GCGGTGGCGC AGCGG

Details for BuviA.01673.j.A2.GE32445

HARVESTED ON:	data unavailable
SEQUENCED ON:	11/11/2011
EXPECTED MW:	51kDa
OBSERVED MW:	30kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	High Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	100
PERCENT COVERAGE:	51

Validated AA Sequence

Validated NT Sequence

attttgtttn nctttaagaa ggagatatac catggctcat caccatcacc atcatatggg taccctggaa gctcagaccc agggtcctgg ttcgatgctg gacccgacgt accagcggcc cgcagcgccg atcgacgaca cgtggtccac cgcggccgcg ccggcgcagc cgcagacggc gcagagcacc gctgcggcac cgctgggtgc cgacctcggc tggcgcgact tcttcaagga cccgcgcctg caaaagctga tcgaactcgc gctcgcgaac aaccgcgata tgcgcgtggc cgcgctcaac gtggcgcagt acgaagcgca gtaccgcatc gcgcgcgcca acctcgggcc ggccatcgac gcgtcggggt ccgtcacgcg cgaacacatg gccggctcca cgagcgcata cagttcgagc agcgcgagcg tgggtctcac gtcgtgggaa atcgacttct tcggccgcct gcgcagcctg aagcgccagg cgctggaaca gtacctcgcc acggatgccg cgcgcaccag cacgcagatc agcctcatcg cgaccgtcgc caccgactat ctgcaactgc tctccgacga ggcttcgttg cagatcgcgc agaacaccgt ggccgccgac cagcacacct acgacctcac ggtgaacatg atgaagctgg gcagcgcgtc gctgcaggac gtgcgccagg ccgaaaactc gctggcgagc gcgcgctcga gcctcgcctc gtacacgcgt gcggtggcgc agcggtaata aacagcacga acaagttctg cagccaagct tctcgaggan ccggctgcta acaaagcccg aaaggaagct ganttggctg ctgcncnaan aannnnnntt ttcnnncccn nnggnnntnc nntcnnccnt nggatcccnc nnngnnnncg ngggnnnnnn ntcncnnnnt nnnnncngna tacnnnnatn nnnnngnncn anngnnnnng ncncnnnnnn nnnnnngnnn nnnnnnnnnn anncgnnnnn ccnnnnnnnc nngngnnnnn nnnnnnncnn nnnnnnnnnn nggnnnggnn nnnnnnnnnn nnncnnnnnn gnncnnnngt cnnnnnnnnn aannnnannn nnnnnnnnnn nnnnnnnnnn nnnnntnnnn nnntnnnnnn cc

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMLDPTYQRP AAPIDDTWST AAAPAQPQTA QSTAAAPLGA DLGWRDFFKD PRLQKLIELA LANNRDMRVA ALNVAQYEAQ YRIARANLGP AIDASGSVTR EHMAGSTSAY SSSSASVGLT SWEIDFFGRL RSLKRQALEQ YLATDAARTS TQISLIATVA TDYLQLLSDE ASLQIAQNTV AADQHTYDLT VNMMKLGSAS LQDVRQAENS LASARSSLAS YTRAVAQDRN DLVAEIGAPL PDDLPQGPSL MESEHTLADI GAGVPSDLLA RRPDIVEAEH TLKAANANIG AARAAFFPKI ELTASAGTTS SGLSNLFKAG TGAWAFAPSI SVPIFDFGYN RASLDVAKVE KDIDVANYEK AIQTAFKEVS NALAGRTTYV TQVAADRDYV SSAQQYYDLA QAHYRSGTDS FLTLLDAQRT LYTAQQQLVT DMLAKQSNLI TLYKVLGGGW SETSAVAQR

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gctggacccg acgtaccagc ggcccgcagc gccgatcgac gacacgtggt ccaccgcggc cgcgccggcg cagccgcaga cggcgcagag caccgctgcg gcaccgctgg gtgccgacct cggctggcgc gacttcttca aggacccgcg cctgcaaaag ctgatcgaac tcgcgctcgc gaacaaccgc gatatgcgcg tggccgcgct caacgtggcg cagtacgaag cgcagtaccg catcgcgcgc gccaacctcg ggccggccat cgacgcgtcg gggtccgtca cgcgcgaaca catggccggc tccacgagcg catacagttc gagcagcgcg agcgtgggtc tcacgtcgtg ggaaatcgac ttcttcggcc gcctgcgcag cctgaagcgc caggcgctgg aacagtacct cgccacggat gccgcgcgca ccagcacgca gatcagcctc atcgcgaccg tcgccaccga ctatctgcaa ctgctctccg acgaggcttc gttgcagatc gcgcagaaca ccgtggccgc cgaccagcac acctacgacc tcacggtgaa catgatgaag ctgggcagcg cgtcgctgca ggacgtgcgc caggccgaaa actcgctggc gagcgcgcgc tcgagcctcg cctcgtacac gcgtgcggtg gcgcaggacc gcaacgacct ggtggccgaa atcggcgcgc cgctgcccga cgatctgccc cagggcccct cgctgatgga aagcgagcac accctcgcgg acatcggcgc gggcgtgccg tcggatctgc tcgcgcgccg gcccgacatc gtcgaagcag agcacacgct caaggcggcc aacgccaaca tcggcgcggc gcgcgccgcg ttctttccga agatcgagct gacggcttcc gcgggcacca cgagcagcgg cctctcgaac ctcttcaagg cgggcacggg cgcgtgggcg tttgcgccgt ccatctcggt gcccatcttc gacttcggct acaaccgcgc gtcgctggac gtggcgaaag tcgaaaagga catcgacgtc gccaactacg aaaaggccat tcagacggcg ttcaaggaag tgtcgaatgc gctggcggga cgcacgacgt acgtcacgca ggtggccgcg gaccgcgact acgtgagctc ggcgcagcag tactacgacc tcgcgcaggc acactaccgc agcggcaccg acagcttcct gaccctgctc gacgcgcagc gcacgcttta cacggcgcaa cagcagctcg tcaccgacat gctcgccaag cagtcgaacc tgatcacgct gtacaaggta ctgggcggcg gctggtcgga gacgagcgcg gtggcgcagc ggtaaacagc acgaacaagt tctgcagcca agcttctcga ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaa