BuviA.01673.h

RND efflux system, outer membrane lipoprotein, NodT family

CENTER ID: BuviA.01673.h
ORGANISM: Burkholderia vietnamiensis G4
ASSOCIATED DISEASE:
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BuviA.01673.h.A2.GE32443 Mature protein( BuviA.01673.h ) 20 470
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|269482.11.peg.2604
RefSeq: YP_001116332.1
UniProt: A4JKR4

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MVSVRKAWIA ALGLALAACA EVGPDYRVPP AALANAPAAS GAFVAGATAA SNAPLPARWW RLYDDPALDA LIERALASNV DLRAAQANLE RSSALLAAAR TAREPSAGFD ASTNYTQQSA AAVLSHVEPP RHEIYNIGFT MSYDLDLFGG IRRGIEAASA DHEAVAAARD LVRVNVAAEV ARAYSDLCNA GNRIDVLQRA IALQQQRLAL TRQLIANGRA PSFEAQRDSG AIDDSRAALA PLQARRLNAA FRLATLMGEP PENYDRALLS CRKPLELAAP IPSGDGRALL ARRPDVRAAE RRLAASTARI GVETAALYPD IKLGASIGST GGAAAFLTPL ANRFAVGPMV TWNLHRSAIR DRIDAARADS RASLAHFDAT VLTALRETET SLSTYAAALE RLQRLQAARD AARDVMERTD ELRHGGRVGG LVALDARRTW LAAETAVAAA RQQVNDDQIA TFLALGGGWQ
NT Sequence
ATGGTGAGCG TGCGCAAAGC GTGGATCGCC GCGCTCGGCC TCGCGCTCGC CGCCTGCGCG GAGGTCGGCC CGGACTATCG CGTGCCGCCT GCCGCGCTCG CGAACGCGCC GGCCGCCAGC GGCGCGTTCG TTGCGGGTGC AACGGCCGCG TCGAATGCGC CGTTGCCCGC TCGCTGGTGG CGGCTGTACG ACGATCCGGC GCTCGACGCG CTGATCGAGC GGGCGCTCGC GAGCAACGTC GATCTGCGCG CCGCGCAGGC GAACCTCGAG CGCAGCAGCG CGCTGCTCGC GGCGGCGCGC ACGGCGCGCG AGCCGAGCGC GGGCTTCGAC GCGTCGACGA ACTACACGCA GCAATCCGCC GCAGCGGTGC TGTCGCACGT CGAGCCGCCG CGGCACGAGA TCTACAACAT CGGCTTCACG ATGAGCTACG ACCTCGATCT GTTCGGCGGC ATCCGGCGCG GGATCGAGGC GGCGTCCGCC GACCACGAGG CCGTCGCCGC CGCGCGCGAT CTGGTGCGCG TGAACGTCGC CGCCGAAGTC GCGCGCGCGT ATTCGGACCT GTGCAATGCC GGAAACCGGA TCGACGTGCT GCAGCGGGCG ATCGCGCTGC AGCAGCAGCG CCTCGCGTTG ACGCGCCAGT TGATCGCTAA CGGCCGCGCG CCGAGCTTCG AAGCGCAGCG CGACAGCGGT GCGATCGACG ACAGCCGCGC GGCGCTCGCG CCGCTGCAGG CGCGCCGGCT GAATGCGGCG TTCCGGCTCG CGACGCTGAT GGGCGAGCCG CCGGAGAACT ACGATCGCGC GCTGCTGTCG TGCCGCAAGC CGCTCGAACT GGCCGCGCCG ATTCCGTCCG GCGACGGCCG CGCGCTGCTC GCGCGCCGTC CGGACGTGCG CGCGGCCGAG CGCCGGCTTG CCGCGTCGAC CGCGCGCATC GGCGTCGAAA CCGCGGCGCT GTATCCGGAC ATCAAGCTCG GCGCGAGCAT CGGCTCGACC GGCGGCGCGG CCGCGTTCCT CACGCCGTTG GCGAACCGCT TCGCGGTCGG GCCGATGGTG ACCTGGAATC TGCATCGCAG CGCGATCCGC GACCGCATCG ACGCGGCCCG CGCCGACAGC CGCGCGAGCC TCGCGCATTT CGATGCGACG GTGCTCACGG CGTTGCGCGA AACGGAGACG TCGCTGAGCA CGTACGCCGC CGCGCTCGAA CGCCTGCAAC GTCTGCAGGC AGCGCGCGAC GCCGCGCGCG ACGTGATGGA GCGTACCGAC GAACTGCGCC ATGGCGGCCG GGTCGGCGGC CTCGTCGCGC TCGATGCGCG GCGCACGTGG CTCGCGGCCG AAACGGCCGT CGCCGCGGCA CGGCAGCAGG TGAACGACGA CCAGATCGCG ACCTTCCTCG CGCTCGGAGG CGGCTGGCAA
Details for BuviA.01673.h.A2.GE32443
HARVESTED ON: 11/8/2011
SEQUENCED ON: 11/11/2011
EXPECTED MW: 50kDa
OBSERVED MW: 50kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 80
PERCENT COVERAGE: 82
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMAEVGPDYR VPPAALANAP AASGAFVAGA TAASNAPLPA RWWRLYDDPA LDALIERALA SNVDLRAAQA NLERSSALLA AARTAREPSA GFDASTNYTQ QSAAAVLSHV EPPRHEIYNI GFTMSYDLDL FGGIRRGIEA ASADHEAVAA ARDLVRVNVA AEVARAYSDL CNAGNRIDVL QRAIALQQQR LALTRQLIAN GRAPSFEAQR DSGAIDDSRA ALAPLQARRL NAAFRLATLM GEPPENYDRA LLSCRKPLEL AAPIPSGDGR ALLARRPDVR AGRAPACRVD RAHRRXXRGA VSXXXARREH RLDXXRXXVP HXXGXXSXRX DGXXXXHXXX DXXXSXXXXX XXXXXXXXXX XXXXXXXXXX X
Validated NT Sequence
ttttgttnnn tttaagaagg agatatacca tggctcatca ccatcaccat catatgggta ccctggaagc tcagacccag ggtcctggtt cgatggcgga ggtcggcccg gactatcgcg tgccgcctgc cgcgctcgcg aacgcgccgg ccgccagcgg cgcgttcgtt gcgggtgcaa cggccgcgtc gaatgcgccg ttgcccgctc gctggtggcg gctgtacgac gatccggcgc tcgacgcgct gatcgagcgg gcgctcgcga gcaacgtcga tctgcgcgcc gcgcaggcga acctcgagcg cagcagcgcg ctgctcgcgg cggcgcgcac ggcgcgcgag ccgagcgcgg gcttcgacgc gtcgacgaac tacacgcagc aatccgccgc agcggtgctg tcgcacgtcg agccgccgcg gcacgagatc tacaacatcg gcttcacgat gagctacgac ctcgatctgt tcggcggcat ccggcgcggg atcgaggcgg cgtccgccga ccacgaggcc gtcgccgccg cgcgcgatct ggtgcgcgtg aacgtcgccg ccgaagtcgc gcgcgcgtat tcggacctgt gcaatgccgg aaaccggatc gacgtgctgc agcgggcgat cgcgctgcag cagcagcgcc tcgcgttgac gcgccagttg atcgctaacg gccgcgcgcc gagcttcgaa gcgcagcgcg acagcggtgc gatcgacgac agccgcgcgg cgctcgcgcc gctgcaggcg cgccggctga atgcggcgtt ccggctcgcg acgctgatgg gcgagccgcc ggagaactac gatcgcgcgc tgctgtcgtg ccgcaagccg ctcgaactgg ccgcgccnat tccgtccggc gacggccgcg cgctgctcgc gcgccgtccg gacgtgcgcg cgggccgagc gccggcttgc cgcgtcgacc gcgcgcatcg gcgtcnaaan cgcggcgctg tatccngann tcnngctcgg cgngagcatc ggctcgacnn gnggcgnngc nncgttcctc acgncnnngg cnnnnnttcn ngncgggnng atggngnccn ggnnnngcat ngnnnnnnng atcnnnacnc atcnanncnn nncnnnnnnn nnnnnncntn nnnnantnnn annnnnnngg nnnntnnnnn nnnnnnnnnn nannnnnnnn naa
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMAEVGPDYR VPPAALANAP AASGAFVAGA TAASNAPLPA RWWRLYDDPA LDALIERALA SNVDLRAAQA NLERSSALLA AARTAREPSA GFDASTNYTQ QSAAAVLSHV EPPRHEIYNI GFTMSYDLDL FGGIRRGIEA ASADHEAVAA ARDLVRVNVA AEVARAYSDL CNAGNRIDVL QRAIALQQQR LALTRQLIAN GRAPSFEAQR DSGAIDDSRA ALAPLQARRL NAAFRLATLM GEPPENYDRA LLSCRKPLEL AAPIPSGDGR ALLARRPDVR AAERRLAAST ARIGVETAAL YPDIKLGASI GSTGGAAAFL TPLANRFAVG PMVTWNLHRS AIRDRIDAAR ADSRASLAHF DATVLTALRE TETSLSTYAA ALERLQRLQA ARDAARDVME RTDELRHGGR VGGLVALDAR RTWLAAETAV AAARQQVNDD QIATFLALGG GWQ
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggcggaggtc ggcccggact atcgcgtgcc gcctgccgcg ctcgcgaacg cgccggccgc cagcggcgcg ttcgttgcgg gtgcaacggc cgcgtcgaat gcgccgttgc ccgctcgctg gtggcggctg tacgacgatc cggcgctcga cgcgctgatc gagcgggcgc tcgcgagcaa cgtcgatctg cgcgccgcgc aggcgaacct cgagcgcagc agcgcgctgc tcgcggcggc gcgcacggcg cgcgagccga gcgcgggctt cgacgcgtcg acgaactaca cgcagcaatc cgccgcagcg gtgctgtcgc acgtcgagcc gccgcggcac gagatctaca acatcggctt cacgatgagc tacgacctcg atctgttcgg cggcatccgg cgcgggatcg aggcggcgtc cgccgaccac gaggccgtcg ccgccgcgcg cgatctggtg cgcgtgaacg tcgccgccga agtcgcgcgc gcgtattcgg acctgtgcaa tgccggaaac cggatcgacg tgctgcagcg ggcgatcgcg ctgcagcagc agcgcctcgc gttgacgcgc cagttgatcg ctaacggccg cgcgccgagc ttcgaagcgc agcgcgacag cggtgcgatc gacgacagcc gcgcggcgct cgcgccgctg caggcgcgcc ggctgaatgc ggcgttccgg ctcgcgacgc tgatgggcga gccgccggag aactacgatc gcgcgctgct gtcgtgccgc aagccgctcg aactggccgc gccgattccg tccggcgacg gccgcgcgct gctcgcgcgc cgtccggacg tgcgcgcggc cgagcgccgg cttgccgcgt cgaccgcgcg catcggcgtc gaaaccgcgg cgctgtatcc ggacatcaag ctcggcgcga gcatcggctc gaccggcggc gcggccgcgt tcctcacgcc gttggcgaac cgcttcgcgg tcgggccgat ggtgacctgg aatctgcatc gcagcgcgat ccgcgaccgc atcgacgcgg cccgcgccga cagccgcgcg agcctcgcgc atttcgatgc gacggtgctc acggcgttgc gcgaaacgga gacgtcgctg agcacgtacg ccgccgcgct cgaacgcctg caacgtctgc aggcagcgcg cgacgccgcg cgcgacgtga tggagcgtac cgacgaactg cgccatggcg gccgggtcgg cggcctcgtc gcgctcgatg cgcggcgcac gtggctcgcg gccgaaacgg ccgtcgccgc ggcacggcag caggtgaacg acgaccagat cgcgaccttc ctcgcgctcg gaggcggctg gcaataaaca gcacgaacaa gttctgcagc caagcttctc gaggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaa