BuviA.01673.g

RND efflux system, outer membrane lipoprotein, NodT family

CENTER ID: BuviA.01673.g
ORGANISM: Burkholderia vietnamiensis G4
ASSOCIATED DISEASE:
CURRENT STATUS: purified
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BuviA.01673.g.A2.GE32442 Mature protein( BuviA.01673.g ) 37 517
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BuviA.01673.g.A2.PS01333 Mature protein( BuviA.01673.g ) 37 517

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|269482.11.peg.2280
RefSeq: YP_001116030.1
UniProt: A4JJW2

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MLEDNKMDNM HNTNGLVRVA KLAAASTLLA TLLAACAVGP DYKRPDVATP AAFKEAPTLA AGEQAGTWKT AEPADAEHRG EWWKVFGDPV LDALESQALA ANQNLKAAAA RVEEARAATR TARSQWFPQV GVGFGPTREG ASSASQFQPQ GTGPTNATLW RAQGTVSYEA DLFGRVSRNV EASRADQAQS EALFRSVQLA LQADVAQNYF ELRQLDSDQD LYRRTVELRE EALKLVQRRF NEGDISELDV SRAKNELASA QADAVGVARR RAASEHALAI LLGKAPADFA FKETPIVPVA VKIPAGLPSA LLERRPDVAA AERAMAAANA RIGLAKSAYF PKLDITGAFG YEASTLGDLF LWSSRTFLLG PFAGTALTLP LFDGGRRAAG VQQARAQYDE QVANYRQQVL VAFREVEDNL ADLRLLDDQI RAQDAAVNAS RRSATLSRTQ YQEGEVAYLD VIDSERSVLQ SQLQANQLTG AQAVSTVNLI RALGGGWGPA PTAVGDAATG KAEVAAR
NT Sequence
ATGCTTGAGG ACAACAAGAT GGACAACATG CACAACACGA ACGGCCTGGT GCGCGTCGCG AAGCTGGCGG CCGCGAGCAC TCTGCTCGCG ACGCTGCTCG CCGCGTGCGC GGTAGGACCC GACTACAAGC GCCCGGACGT GGCGACGCCC GCGGCGTTCA AGGAAGCGCC GACGCTCGCC GCCGGCGAGC AGGCCGGCAC GTGGAAGACC GCCGAGCCGG CCGACGCCGA GCATCGCGGC GAATGGTGGA AGGTGTTCGG CGATCCGGTG CTCGACGCGC TGGAGTCGCA GGCGCTCGCC GCGAACCAGA ACCTGAAGGC CGCGGCCGCG CGCGTCGAGG AAGCGCGTGC GGCGACCCGC ACCGCTCGCT CGCAGTGGTT CCCGCAGGTC GGCGTCGGGT TCGGGCCGAC GCGCGAGGGG GCGTCGTCGG CGTCGCAGTT CCAGCCGCAG GGCACGGGGC CGACCAACGC GACGCTGTGG CGTGCGCAGG GCACGGTGTC GTACGAGGCC GACCTGTTCG GCCGCGTGAG CCGCAACGTC GAGGCGTCGC GTGCCGACCA GGCGCAGAGC GAGGCGCTGT TCCGTTCGGT GCAGCTCGCG CTGCAGGCGG ACGTCGCGCA GAACTACTTC GAGCTGCGCC AGCTCGATTC GGACCAGGAT CTGTACCGTC GCACGGTCGA GCTGCGCGAA GAGGCGCTGA AGCTCGTGCA GCGCCGCTTC AACGAAGGCG ACATCAGCGA GCTGGACGTG TCGCGTGCGA AGAACGAGCT GGCGAGCGCG CAGGCCGATG CGGTCGGCGT CGCGCGGCGG CGCGCGGCGT CCGAGCATGC GCTCGCGATC CTGCTCGGCA AGGCGCCCGC GGACTTCGCG TTCAAGGAAA CGCCGATCGT GCCGGTCGCG GTGAAGATCC CGGCGGGGCT GCCGTCGGCG CTGCTCGAAC GCCGCCCGGA TGTGGCGGCG GCCGAGCGCG CGATGGCTGC CGCGAACGCG CGGATCGGCC TTGCGAAGTC GGCGTATTTC CCGAAGCTCG ATATCACGGG CGCGTTCGGG TATGAGGCGT CGACGCTCGG CGATCTGTTC CTGTGGTCGA GCCGCACGTT CCTGCTCGGG CCGTTCGCCG GCACCGCGCT GACGCTGCCG CTGTTCGACG GCGGACGTCG TGCGGCCGGC GTGCAGCAGG CGCGCGCGCA GTACGACGAG CAGGTCGCGA ACTACCGGCA GCAGGTGCTC GTCGCGTTCC GCGAGGTCGA GGACAATCTC GCCGATCTGC GGTTGCTCGA CGATCAGATT CGGGCTCAGG ATGCCGCGGT CAATGCGTCG CGCCGTTCGG CGACGCTGTC GCGTACGCAG TATCAGGAAG GGGAAGTGGC GTATCTCGAC GTGATCGATA GCGAGCGGTC GGTGCTGCAG TCGCAATTGC AGGCGAATCA GTTGACTGGG GCGCAGGCGG TTTCGACCGT CAATCTCATT CGTGCGCTGG GCGGCGGATG GGGACCGGCG CCGACGGCGG TCGGCGATGC GGCGACCGGC AAGGCGGAGG TGGCGGCGCG G
Details for BuviA.01673.g.A2.GE32442
HARVESTED ON: 11/8/2011
SEQUENCED ON: 11/11/2011
EXPECTED MW: 54kDa
OBSERVED MW: 54kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 92
PERCENT COVERAGE: 62
Validated AA Sequence
MAVGPDYKRP DVATPAAFKE APTLAAGEQA GTWKTAEPAD AEHRGEWWKV FGDPVLDALE SQALAANQNL KAAAARVEEA RAATRTARSQ WFPQVGVGFG PTREGASSAS QFQPQGTGPT NATLWRAQGT VSYEADLFGR VSRNVEASRA DQAQSEALFR SVQLALQADV AQNYFELRQL DSDQDLYRRT VELREEALKL VQRRFNEGDI SELDVSRAKN ELASAQADAV GVARRRAASE HALAILLGKA PADFAFKETP IVPVAVKIPA GLPSALLERR PGCGGGRARD GCRERADRPC EVGVFPEARY HGRVRV
Validated NT Sequence
accgcgccgc cacctccgcc ttgccggtcg ccgcatcgcc gaccgccgtc ggcgccggtc cccatccgcc gcccagcgca cgaatgagat tgacggtcga aaccgcctgc gccccagtca actgattcgc ctgcaattgc gactgcagca ccgaccgctc gctatcgatc acgtcgagat acgccacttc cccttcctga tactgcgtac gcgacagcgt cgccgaacgg cgcgacgcat tgaccgcggc atcctgagcc cgaatctgat cgtcgagcaa ccgcagatcg gcgagattgt cctcgacctc gcggaacgcg acgagcacct gctgccggta gttcgcgacc tgctcgtcgt actgcgcgcg cgcctgctgc acgccggccg cacgacgtcc gccgtcgaac agcggcagcg tcagcgcggt gccggcgaac ggcccgagca ggaacgtgcg gctcgaccac aggaacagat cgccgagcgt cgacgcctca tacccgaacg cgcccgtgat atcgagcttc gggaaatacg ccgacttcgc aaggccgatc cgcgcgttcg cggcagccat cgcgcgctcg gccgccgcca catccggggc ggcgttcgag cagcgccgac ggcagccccg ccgggatctt caccgcgacc ggcacgatcg gcgtttcctt gaacgcgaag tccgcgggcg ccttgccgag caggatcgcg agcgcatgct cggacgccgc gcgccgccgc gcgacgccga ccgcatcggc ctgcgcgctc gccagctcgt tcttcgcacg cgacacgtcc agctcgctga tgtcgccttc gttgaagcgg cgctgcacga gcttcagcgc ctcttcgcgc agctcgaccg tgcgacggta cagatcctgg tccgaatcga gctggcgcag ctcgaagtag ttctgcgcga cgtccgcctg cagcgcgagc tgcaccgaac ggaacagcgc ctcgctctgc gcctggtcgg cacgcgacgc ctcgacgttg cggctcacgc ggccgaacag gtcggcctcg tacgacaccg tgccctgcgc acgccacagc gtcgcgttgg tcggccccgt gccctgcggc tggaactgcg acgccgacga cgccccctcg cgcgtcggcc cgaacccgac gccgacctgc gggaaccact gcgagcgagc ggtgcgggtc gccgcacgcg cttcctcgac gcgcgcggcc gcggccttca ggttctggtt cgcggcgagc gcctgcgact ccagcgcgtc gagcaccgga tcgccgaaca ccttccacca ttcgccgcga tgctcggcgt cggccggctc ggcggtcttc cacgtgccgg cctgctcgcc ggcggcgagc gtcggcgctt ccttgaacgc cgcgggcgtc gccacgtccg ggcgcttgta gtcgggtcct accgccatcg aaccaggacc ctgggtctga gcttccaggg ta
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMAVGPDYKR PDVATPAAFK EAPTLAAGEQ AGTWKTAEPA DAEHRGEWWK VFGDPVLDAL ESQALAANQN LKAAAARVEE ARAATRTARS QWFPQVGVGF GPTREGASSA SQFQPQGTGP TNATLWRAQG TVSYEADLFG RVSRNVEASR ADQAQSEALF RSVQLALQAD VAQNYFELRQ LDSDQDLYRR TVELREEALK LVQRRFNEGD ISELDVSRAK NELASAQADA VGVARRRAAS EHALAILLGK APADFAFKET PIVPVAVKIP AGLPSALLER RPDVAAAERA MAAANARIGL AKSAYFPKLD ITGAFGYEAS TLGDLFLWSS RTFLLGPFAG TALTLPLFDG GRRAAGVQQA RAQYDEQVAN YRQQVLVAFR EVEDNLADLR LLDDQIRAQD AAVNASRRSA TLSRTQYQEG EVAYLDVIDS ERSVLQSQLQ ANQLTGAQAV STVNLIRALG GGWGPAPTAV GDAATGKAEV AAR
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggcggtagga cccgactaca agcgcccgga cgtggcgacg cccgcggcgt tcaaggaagc gccgacgctc gccgccggcg agcaggccgg cacgtggaag accgccgagc cggccgacgc cgagcatcgc ggcgaatggt ggaaggtgtt cggcgatccg gtgctcgacg cgctggagtc gcaggcgctc gccgcgaacc agaacctgaa ggccgcggcc gcgcgcgtcg aggaagcgcg tgcggcgacc cgcaccgctc gctcgcagtg gttcccgcag gtcggcgtcg ggttcgggcc gacgcgcgag ggggcgtcgt cggcgtcgca gttccagccg cagggcacgg ggccgaccaa cgcgacgctg tggcgtgcgc agggcacggt gtcgtacgag gccgacctgt tcggccgcgt gagccgcaac gtcgaggcgt cgcgtgccga ccaggcgcag agcgaggcgc tgttccgttc ggtgcagctc gcgctgcagg cggacgtcgc gcagaactac ttcgagctgc gccagctcga ttcggaccag gatctgtacc gtcgcacggt cgagctgcgc gaagaggcgc tgaagctcgt gcagcgccgc ttcaacgaag gcgacatcag cgagctggac gtgtcgcgtg cgaagaacga gctggcgagc gcgcaggccg atgcggtcgg cgtcgcgcgg cggcgcgcgg cgtccgagca tgcgctcgcg atcctgctcg gcaaggcgcc cgcggacttc gcgttcaagg aaacgccgat cgtgccggtc gcggtgaaga tcccggcggg gctgccgtcg gcgctgctcg aacgccgccc ggatgtggcg gcggccgagc gcgcgatggc tgccgcgaac gcgcggatcg gccttgcgaa gtcggcgtat ttcccgaagc tcgatatcac gggcgcgttc gggtatgagg cgtcgacgct cggcgatctg ttcctgtggt cgagccgcac gttcctgctc gggccgttcg ccggcaccgc gctgacgctg ccgctgttcg acggcggacg tcgtgcggcc ggcgtgcagc aggcgcgcgc gcagtacgac gagcaggtcg cgaactaccg gcagcaggtg ctcgtcgcgt tccgcgaggt cgaggacaat ctcgccgatc tgcggttgct cgacgatcag attcgggctc aggatgccgc ggtcaatgcg tcgcgccgtt cggcgacgct gtcgcgtacg cagtatcagg aaggggaagt ggcgtatctc gacgtgatcg atagcgagcg gtcggtgctg cagtcgcaat tgcaggcgaa tcagttgact ggggcgcagg cggtttcgac cgtcaatctc attcgtgcgc tgggcggcgg atggggaccg gcgccgacgg cggtcggcga tgcggcgacc ggcaaggcgg aggtggcggc gcggtaaaca gcacgaacaa gttctgcagc caagcttctc gaggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaa
Details for BuviA.01673.g.A2.PS01333
PURIFICATION DATe: 1/5/2012
CONCENTRATION: 53mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 5
VIAL VOLUME: 110µl
PERCENT IDENTITY: 92
PERCENT COVERAGE: 62
Protocol Notes
notes unavailable
Validated AA Sequence
MAVGPDYKRP DVATPAAFKE APTLAAGEQA GTWKTAEPAD AEHRGEWWKV FGDPVLDALE SQALAANQNL KAAAARVEEA RAATRTARSQ WFPQVGVGFG PTREGASSAS QFQPQGTGPT NATLWRAQGT VSYEADLFGR VSRNVEASRA DQAQSEALFR SVQLALQADV AQNYFELRQL DSDQDLYRRT VELREEALKL VQRRFNEGDI SELDVSRAKN ELASAQADAV GVARRRAASE HALAILLGKA PADFAFKETP IVPVAVKIPA GLPSALLERR PGCGGGRARD GCRERADRPC EVGVFPEARY HGRVRV
Validated NT Sequence
accgcgccgc cacctccgcc ttgccggtcg ccgcatcgcc gaccgccgtc ggcgccggtc cccatccgcc gcccagcgca cgaatgagat tgacggtcga aaccgcctgc gccccagtca actgattcgc ctgcaattgc gactgcagca ccgaccgctc gctatcgatc acgtcgagat acgccacttc cccttcctga tactgcgtac gcgacagcgt cgccgaacgg cgcgacgcat tgaccgcggc atcctgagcc cgaatctgat cgtcgagcaa ccgcagatcg gcgagattgt cctcgacctc gcggaacgcg acgagcacct gctgccggta gttcgcgacc tgctcgtcgt actgcgcgcg cgcctgctgc acgccggccg cacgacgtcc gccgtcgaac agcggcagcg tcagcgcggt gccggcgaac ggcccgagca ggaacgtgcg gctcgaccac aggaacagat cgccgagcgt cgacgcctca tacccgaacg cgcccgtgat atcgagcttc gggaaatacg ccgacttcgc aaggccgatc cgcgcgttcg cggcagccat cgcgcgctcg gccgccgcca catccggggc ggcgttcgag cagcgccgac ggcagccccg ccgggatctt caccgcgacc ggcacgatcg gcgtttcctt gaacgcgaag tccgcgggcg ccttgccgag caggatcgcg agcgcatgct cggacgccgc gcgccgccgc gcgacgccga ccgcatcggc ctgcgcgctc gccagctcgt tcttcgcacg cgacacgtcc agctcgctga tgtcgccttc gttgaagcgg cgctgcacga gcttcagcgc ctcttcgcgc agctcgaccg tgcgacggta cagatcctgg tccgaatcga gctggcgcag ctcgaagtag ttctgcgcga cgtccgcctg cagcgcgagc tgcaccgaac ggaacagcgc ctcgctctgc gcctggtcgg cacgcgacgc ctcgacgttg cggctcacgc ggccgaacag gtcggcctcg tacgacaccg tgccctgcgc acgccacagc gtcgcgttgg tcggccccgt gccctgcggc tggaactgcg acgccgacga cgccccctcg cgcgtcggcc cgaacccgac gccgacctgc gggaaccact gcgagcgagc ggtgcgggtc gccgcacgcg cttcctcgac gcgcgcggcc gcggccttca ggttctggtt cgcggcgagc gcctgcgact ccagcgcgtc gagcaccgga tcgccgaaca ccttccacca ttcgccgcga tgctcggcgt cggccggctc ggcggtcttc cacgtgccgg cctgctcgcc ggcggcgagc gtcggcgctt ccttgaacgc cgcgggcgtc gccacgtccg ggcgcttgta gtcgggtcct accgccatcg aaccaggacc ctgggtctga gcttccaggg ta
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMAVGPDYKR PDVATPAAFK EAPTLAAGEQ AGTWKTAEPA DAEHRGEWWK VFGDPVLDAL ESQALAANQN LKAAAARVEE ARAATRTARS QWFPQVGVGF GPTREGASSA SQFQPQGTGP TNATLWRAQG TVSYEADLFG RVSRNVEASR ADQAQSEALF RSVQLALQAD VAQNYFELRQ LDSDQDLYRR TVELREEALK LVQRRFNEGD ISELDVSRAK NELASAQADA VGVARRRAAS EHALAILLGK APADFAFKET PIVPVAVKIP AGLPSALLER RPDVAAAERA MAAANARIGL AKSAYFPKLD ITGAFGYEAS TLGDLFLWSS RTFLLGPFAG TALTLPLFDG GRRAAGVQQA RAQYDEQVAN YRQQVLVAFR EVEDNLADLR LLDDQIRAQD AAVNASRRSA TLSRTQYQEG EVAYLDVIDS ERSVLQSQLQ ANQLTGAQAV STVNLIRALG GGWGPAPTAV GDAATGKAEV AAR
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGCGGTAGGA CCCGACTACA AGCGCCCGGA CGTGGCGACG CCCGCGGCGT TCAAGGAAGC GCCGACGCTC GCCGCCGGCG AGCAGGCCGG CACGTGGAAG ACCGCCGAGC CGGCCGACGC CGAGCATCGC GGCGAATGGT GGAAGGTGTT CGGCGATCCG GTGCTCGACG CGCTGGAGTC GCAGGCGCTC GCCGCGAACC AGAACCTGAA GGCCGCGGCC GCGCGCGTCG AGGAAGCGCG TGCGGCGACC CGCACCGCTC GCTCGCAGTG GTTCCCGCAG GTCGGCGTCG GGTTCGGGCC GACGCGCGAG GGGGCGTCGT CGGCGTCGCA GTTCCAGCCG CAGGGCACGG GGCCGACCAA CGCGACGCTG TGGCGTGCGC AGGGCACGGT GTCGTACGAG GCCGACCTGT TCGGCCGCGT GAGCCGCAAC GTCGAGGCGT CGCGTGCCGA CCAGGCGCAG AGCGAGGCGC TGTTCCGTTC GGTGCAGCTC GCGCTGCAGG CGGACGTCGC GCAGAACTAC TTCGAGCTGC GCCAGCTCGA TTCGGACCAG GATCTGTACC GTCGCACGGT CGAGCTGCGC GAAGAGGCGC TGAAGCTCGT GCAGCGCCGC TTCAACGAAG GCGACATCAG CGAGCTGGAC GTGTCGCGTG CGAAGAACGA GCTGGCGAGC GCGCAGGCCG ATGCGGTCGG CGTCGCGCGG CGGCGCGCGG CGTCCGAGCA TGCGCTCGCG ATCCTGCTCG GCAAGGCGCC CGCGGACTTC GCGTTCAAGG AAACGCCGAT CGTGCCGGTC GCGGTGAAGA TCCCGGCGGG GCTGCCGTCG GCGCTGCTCG AACGCCGCCC GGATGTGGCG GCGGCCGAGC GCGCGATGGC TGCCGCGAAC GCGCGGATCG GCCTTGCGAA GTCGGCGTAT TTCCCGAAGC TCGATATCAC GGGCGCGTTC GGGTATGAGG CGTCGACGCT CGGCGATCTG TTCCTGTGGT CGAGCCGCAC GTTCCTGCTC GGGCCGTTCG CCGGCACCGC GCTGACGCTG CCGCTGTTCG ACGGCGGACG TCGTGCGGCC GGCGTGCAGC AGGCGCGCGC GCAGTACGAC GAGCAGGTCG CGAACTACCG GCAGCAGGTG CTCGTCGCGT TCCGCGAGGT CGAGGACAAT CTCGCCGATC TGCGGTTGCT CGACGATCAG ATTCGGGCTC AGGATGCCGC GGTCAATGCG TCGCGCCGTT CGGCGACGCT GTCGCGTACG CAGTATCAGG AAGGGGAAGT GGCGTATCTC GACGTGATCG ATAGCGAGCG GTCGGTGCTG CAGTCGCAAT TGCAGGCGAA TCAGTTGACT GGGGCGCAGG CGGTTTCGAC CGTCAATCTC ATTCGTGCGC TGGGCGGCGG ATGGGGACCG GCGCCGACGG CGGTCGGCGA TGCGGCGACC GGCAAGGCGG AGGTGGCGGC GCGGTAAACA GCACGAACAA GTTCTGCAGC CAAGCTTCTC GAGGATCCGG CTGCTAACAA AGCCCGAAAG GAAGCTGAGT TGGCTGCTGC CACCGCTGAG CAATAACTAG CATAACCCCT TGGGGCCTCT AAACGGGTCT TGAGGGGTTT TTTGCTGAAA GGAGGAACTA TATCCGGATA TCCACAGGAC GGGTGTGGTC GCCATGATCG CGTAGTCGAT AGTGGCTCCA AGTAGCGAAG CGAGCAGGAC TGGGCGGCGG CCAAAGCGGT CGGACAGTGC TCCGAGAACG GGTGCGCATA GAAATTGCAT CAACGCATAT AGCGCTAGCA GCACGCCATA GTGACTGGCG ATGCTGTCGG AATGGACGAT ATCCCGCAAG AGGCCCGGCA GTACCGGCAT AACCAAGCCT ATGCCTACAG CATCCAGGGT GACGGTGCCG AGGATGACGA TGAGCGCATT GTTAGATTTC ATACACGGTG CCTGACTGCG TTAGCAATTT AACTGTGATA AACTACCGCA TTAAAGCTTA TCGATGATAA GCTGTCAAAC ATGAGAA