BuviA.01673.c

RND efflux system, outer membrane lipoprotein, NodT family

CENTER ID: BuviA.01673.c
ORGANISM: Burkholderia vietnamiensis G4
ASSOCIATED DISEASE:
CURRENT STATUS: purified
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BuviA.01673.c.A2.GE32438 Mature protein( BuviA.01673.c ) 29 501
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BuviA.01673.c.A2.PW32358 Mature protein( BuviA.01673.c ) 29 501

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|269482.11.peg.5703
RefSeq: YP_001119314.1
UniProt: A4JDX6

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MKSSTLSVRA GSCRTAVAAA VAALALAGCA NYIGIKSDKQ IAPASQFESA QSLPAQGGQW PTLDWASQFG DPQLPKLIDE ALQGNPSIAQ AQARIAKASS YIESSRSNLM PKAEASYSWT RELYSSNALF PPPYGGQWYS ENNVLASASW DLDLWGKNRE RLHTAVSQEK AAEADMQQAR ITLASSVART YNSLAQLYAL RDIAQREITN RQTVGKITDG RVSAGLDTNV ERQTARGNIA TTQASLSDLD GQITTVRYQL AALLGKGPDR GLQIAAPVMN PSGTVALPGN LPADLVARRP DIVAARWQVE AAMHDVKEAK AEFFPDVNLA AGFGFDAFGW GKFLNFASRQ AQFGPAIHLP IFDAGALRAQ LKGRYADFDL SVANYNQTLI GALNDVATQV ASIRAVDRQM DDAQRALDAS TRAYDLAVIR YKAGLSPQLQ VLTADSNRLA SEQTVTNLKM RRRDMQLALI KALGGGFDAT GTRLAAPDAE QQKTQTKQAA N
NT Sequence
ATGAAATCCT CCACGTTGTC CGTGCGCGCC GGGTCGTGCC GCACCGCCGT CGCCGCCGCA GTTGCCGCGC TGGCGCTGGC GGGCTGCGCA AACTATATCG GCATCAAGAG CGACAAGCAG ATCGCGCCAG CGTCGCAATT CGAATCGGCG CAAAGCCTGC CAGCCCAGGG CGGCCAGTGG CCGACGCTCG ACTGGGCCAG CCAGTTCGGC GATCCGCAGC TGCCGAAGCT GATCGACGAG GCGCTCCAGG GCAATCCATC GATCGCGCAG GCGCAGGCGC GGATCGCGAA GGCGTCGTCG TATATCGAAT CGTCGCGCTC GAACCTGATG CCGAAGGCCG AAGCGAGCTA CTCGTGGACG CGCGAGCTCT ATTCTTCCAA TGCGCTGTTC CCGCCCCCGT ACGGCGGCCA GTGGTACAGC GAGAACAATG TGCTCGCGAG CGCGTCGTGG GATCTCGACC TGTGGGGCAA GAATCGCGAG CGGCTGCACA CCGCCGTGTC GCAGGAAAAG GCCGCCGAAG CGGACATGCA GCAGGCGCGC ATCACGCTCG CGTCGTCGGT CGCCCGCACC TATAACTCGC TCGCGCAACT GTATGCGCTG CGCGACATCG CGCAGCGCGA GATCACCAAC CGTCAGACGG TCGGCAAGAT CACCGACGGT CGCGTGTCGG CCGGCCTCGA CACTAATGTC GAGCGTCAGA CCGCCCGCGG CAACATCGCG ACGACGCAGG CGTCGCTGTC CGACCTCGAC GGCCAGATCA CGACCGTGCG CTACCAGCTC GCCGCGCTGC TCGGCAAGGG GCCGGACCGC GGGCTGCAGA TCGCCGCGCC GGTGATGAAC CCGTCCGGCA CGGTCGCCCT GCCCGGCAAC CTGCCGGCCG ACCTCGTGGC GCGCCGCCCC GACATCGTCG CCGCGCGCTG GCAGGTGGAA GCCGCGATGC ACGACGTGAA GGAGGCGAAG GCCGAGTTCT TCCCCGACGT GAACCTCGCG GCCGGCTTCG GCTTCGACGC GTTCGGCTGG GGCAAATTCC TGAACTTCGC GAGCCGCCAG GCGCAGTTCG GCCCGGCCAT CCACCTGCCG ATCTTCGATG CCGGCGCATT GCGTGCGCAG CTCAAGGGCC GCTATGCGGA CTTCGACCTG TCGGTCGCGA ACTACAACCA GACGCTGATC GGCGCGCTGA ACGACGTGGC GACGCAGGTC GCGTCGATCC GCGCGGTCGA CCGCCAGATG GACGACGCGC AGCGTGCGCT CGACGCATCG ACGCGCGCCT ACGACCTGGC CGTGATCCGC TACAAGGCCG GCCTGTCGCC GCAGCTGCAG GTGCTCACCG CCGACAGCAA CCGCCTCGCA TCGGAACAGA CGGTCACGAA CCTGAAGATG CGCCGGCGCG ACATGCAGCT CGCGCTGATC AAGGCGCTCG GCGGCGGCTT CGACGCAACC GGCACGCGGC TCGCGGCGCC CGACGCAGAA CAACAGAAAA CGCAAACAAA GCAGGCCGCC AAC
Details for BuviA.01673.c.A2.GE32438
HARVESTED ON: 11/8/2011
SEQUENCED ON: 11/11/2011
EXPECTED MW: 53kDa
OBSERVED MW: 53kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 100
PERCENT COVERAGE: 98
Validated AA Sequence
MGTLEAQTQG PGSMCANYIG IKSDKQIAPA SQFESAQSLP AQGGQWPTLD WASQFGDPQL PKLIDEALQG NPSIAQAQAR IAKASSYIES SRSNLMPKAE ASYSWTRELY SSNALFPPPY GGQWYSENNV LASASWDLDL WGKNRERLHT AVSQEKAAEA DMQQARITLA SSVARTYNSL AQLYALRDIA QREITNRQTV GKITDGRVSA GLDTNVERQT ARGNIATTQA SLSDLDGQIT TVRYQLAALL GKGPDRGLQI AAPVMNPSGT VALPGNLPAD LVARRPDIVA ARWQVEAAMH DVKEAKAEFF PDVNLAAGFG FDAFGWGKFL NFASRQAQFG PAIHLPIFDA GALRAQLKGR YADFDLSVAN YNQTLIGALN DVATQVASIR AVDRQMDDAQ RALDASTRAY DLAVIRYKAG LSPQLQVLTA DSNRLASEQT VTNLKMRRRD MQLALIKALG GGFDATGTRL AAPDAEQQKT QTKQAAN
Validated NT Sequence
agttggcggc ctgctttgtt tgcgttttct gttgttctgc gtcgggcgcc gcgagccgcg tgccggttgc gtcgaagccg ccgccgagcg ccttgatcag cgcgagctgc atgtcgcgcc ggcgcatctt caggttcgtg accgtctgtt ccgatgcgag gcggttgctg tcggcggtga gcacctgcag ctgcggcgac aggccggcct tgtagcggat cacggccagg tcgtaggcgc gcgtcgatgc gtcgagcgca cgctgcgcgt cgtccatctg gcggtcgacc gcgcggatcg acgcgacctg cgtcgccacg tcgttcagcg cgccgatcag cgtctggttg tagttcgcga ccgacaggtc gaagtccgca tagcggccct tgagctgcgc acgcaatgcg ccggcatcga agatcggcag gtggatggcc gggccgaact gcgcctggcg gctcgcgaag ttcaggaatt tgccccagcc gaacgcgtcg aagccgaagc cggccgcgag gttcacgtcg gggaagaact cggccttcgc ctccttcacg tcgtgcatcg cggcttccac ctgccagcgc gcggcgacga tgtcggggcg gcgcgccacg aggtcggccg gcaggttgcc gggcagggcg accgtgccgg acgggttcat caccggcgcg gcgatctgca gcccgcggtc cggccccttg ccgagcagcg cggcgagctg gtagcgcacg gtcgtgatct ggccgtcgag gtcggacagc gacgcctgcg tcgtcgcgat gttgccgcgg gcggtctgac gctcgacatt agtgtcgagg ccggccgaca cgcgaccgtc ggtgatcttg ccgaccgtct gacggttggt gatctcgcgc tgcgcgatgt cgcgcagcgc atacagttgc gcgagcgagt tataggtgcg ggcgaccgac gacgcgagcg tgatgcgcgc ctgctgcatg tccgcttcgg cggccttttc ctgcgacacg gcggtgtgca gccgctcgcg attcttgccc cacaggtcga gatcccacga cgcgctcgcg agcacattgt tctcgctgta ccactggccg ccgtacgggg gcgggaacag cgcattggaa gaatagagct cgcgcgtcca cgagtagctc gcttcggcct tcggcatcag gttcgagcgc gacgattcga tatacgacga cgccttcgcg atccgcgcct gcgcctgcgc gatcgatgga ttgccctgga gcgcctcgtc gatcagcttc ggcagctgcg gatcgccgaa ctggctggcc cagtcgagcg tcggccactg gccgccctgg gctggcaggc tttgcgccga ttcgaattgc gacgctggcg cgatctgctt gtcgctcttg atgccgatat agtttgcgca catcgaacca ggaccctggg tctgagcttc cagggtaccc at
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMCANYIGIK SDKQIAPASQ FESAQSLPAQ GGQWPTLDWA SQFGDPQLPK LIDEALQGNP SIAQAQARIA KASSYIESSR SNLMPKAEAS YSWTRELYSS NALFPPPYGG QWYSENNVLA SASWDLDLWG KNRERLHTAV SQEKAAEADM QQARITLASS VARTYNSLAQ LYALRDIAQR EITNRQTVGK ITDGRVSAGL DTNVERQTAR GNIATTQASL SDLDGQITTV RYQLAALLGK GPDRGLQIAA PVMNPSGTVA LPGNLPADLV ARRPDIVAAR WQVEAAMHDV KEAKAEFFPD VNLAAGFGFD AFGWGKFLNF ASRQAQFGPA IHLPIFDAGA LRAQLKGRYA DFDLSVANYN QTLIGALNDV ATQVASIRAV DRQMDDAQRA LDASTRAYDL AVIRYKAGLS PQLQVLTADS NRLASEQTVT NLKMRRRDMQ LALIKALGGG FDATGTRLAA PDAEQQKTQT KQAAN
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gtgcgcaaac tatatcggca tcaagagcga caagcagatc gcgccagcgt cgcaattcga atcggcgcaa agcctgccag cccagggcgg ccagtggccg acgctcgact gggccagcca gttcggcgat ccgcagctgc cgaagctgat cgacgaggcg ctccagggca atccatcgat cgcgcaggcg caggcgcgga tcgcgaaggc gtcgtcgtat atcgaatcgt cgcgctcgaa cctgatgccg aaggccgaag cgagctactc gtggacgcgc gagctctatt cttccaatgc gctgttcccg cccccgtacg gcggccagtg gtacagcgag aacaatgtgc tcgcgagcgc gtcgtgggat ctcgacctgt ggggcaagaa tcgcgagcgg ctgcacaccg ccgtgtcgca ggaaaaggcc gccgaagcgg acatgcagca ggcgcgcatc acgctcgcgt cgtcggtcgc ccgcacctat aactcgctcg cgcaactgta tgcgctgcgc gacatcgcgc agcgcgagat caccaaccgt cagacggtcg gcaagatcac cgacggtcgc gtgtcggccg gcctcgacac taatgtcgag cgtcagaccg cccgcggcaa catcgcgacg acgcaggcgt cgctgtccga cctcgacggc cagatcacga ccgtgcgcta ccagctcgcc gcgctgctcg gcaaggggcc ggaccgcggg ctgcagatcg ccgcgccggt gatgaacccg tccggcacgg tcgccctgcc cggcaacctg ccggccgacc tcgtggcgcg ccgccccgac atcgtcgccg cgcgctggca ggtggaagcc gcgatgcacg acgtgaagga ggcgaaggcc gagttcttcc ccgacgtgaa cctcgcggcc ggcttcggct tcgacgcgtt cggctggggc aaattcctga acttcgcgag ccgccaggcg cagttcggcc cggccatcca cctgccgatc ttcgatgccg gcgcattgcg tgcgcagctc aagggccgct atgcggactt cgacctgtcg gtcgcgaact acaaccagac gctgatcggc gcgctgaacg acgtggcgac gcaggtcgcg tcgatccgcg cggtcgaccg ccagatggac gacgcgcagc gtgcgctcga cgcatcgacg cgcgcctacg acctggccgt gatccgctac aaggccggcc tgtcgccgca gctgcaggtg ctcaccgccg acagcaaccg cctcgcatcg gaacagacgg tcacgaacct gaagatgcgc cggcgcgaca tgcagctcgc gctgatcaag gcgctcggcg gcggcttcga cgcaaccggc acgcggctcg cggcgcccga cgcagaacaa cagaaaacgc aaacaaagca ggccgccaac taaacagcac gaacaagttc tgcagccaag cttctcgagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaa
Details for BuviA.01673.c.A2.PW32358
PURIFICATION DATe: 12/16/2011
CONCENTRATION: 37.42mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: High Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 2
VIAL VOLUME: 200µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 98
Protocol Notes
notes unavailable
Validated AA Sequence
MGTLEAQTQG PGSMCANYIG IKSDKQIAPA SQFESAQSLP AQGGQWPTLD WASQFGDPQL PKLIDEALQG NPSIAQAQAR IAKASSYIES SRSNLMPKAE ASYSWTRELY SSNALFPPPY GGQWYSENNV LASASWDLDL WGKNRERLHT AVSQEKAAEA DMQQARITLA SSVARTYNSL AQLYALRDIA QREITNRQTV GKITDGRVSA GLDTNVERQT ARGNIATTQA SLSDLDGQIT TVRYQLAALL GKGPDRGLQI AAPVMNPSGT VALPGNLPAD LVARRPDIVA ARWQVEAAMH DVKEAKAEFF PDVNLAAGFG FDAFGWGKFL NFASRQAQFG PAIHLPIFDA GALRAQLKGR YADFDLSVAN YNQTLIGALN DVATQVASIR AVDRQMDDAQ RALDASTRAY DLAVIRYKAG LSPQLQVLTA DSNRLASEQT VTNLKMRRRD MQLALIKALG GGFDATGTRL AAPDAEQQKT QTKQAAN
Validated NT Sequence
agttggcggc ctgctttgtt tgcgttttct gttgttctgc gtcgggcgcc gcgagccgcg tgccggttgc gtcgaagccg ccgccgagcg ccttgatcag cgcgagctgc atgtcgcgcc ggcgcatctt caggttcgtg accgtctgtt ccgatgcgag gcggttgctg tcggcggtga gcacctgcag ctgcggcgac aggccggcct tgtagcggat cacggccagg tcgtaggcgc gcgtcgatgc gtcgagcgca cgctgcgcgt cgtccatctg gcggtcgacc gcgcggatcg acgcgacctg cgtcgccacg tcgttcagcg cgccgatcag cgtctggttg tagttcgcga ccgacaggtc gaagtccgca tagcggccct tgagctgcgc acgcaatgcg ccggcatcga agatcggcag gtggatggcc gggccgaact gcgcctggcg gctcgcgaag ttcaggaatt tgccccagcc gaacgcgtcg aagccgaagc cggccgcgag gttcacgtcg gggaagaact cggccttcgc ctccttcacg tcgtgcatcg cggcttccac ctgccagcgc gcggcgacga tgtcggggcg gcgcgccacg aggtcggccg gcaggttgcc gggcagggcg accgtgccgg acgggttcat caccggcgcg gcgatctgca gcccgcggtc cggccccttg ccgagcagcg cggcgagctg gtagcgcacg gtcgtgatct ggccgtcgag gtcggacagc gacgcctgcg tcgtcgcgat gttgccgcgg gcggtctgac gctcgacatt agtgtcgagg ccggccgaca cgcgaccgtc ggtgatcttg ccgaccgtct gacggttggt gatctcgcgc tgcgcgatgt cgcgcagcgc atacagttgc gcgagcgagt tataggtgcg ggcgaccgac gacgcgagcg tgatgcgcgc ctgctgcatg tccgcttcgg cggccttttc ctgcgacacg gcggtgtgca gccgctcgcg attcttgccc cacaggtcga gatcccacga cgcgctcgcg agcacattgt tctcgctgta ccactggccg ccgtacgggg gcgggaacag cgcattggaa gaatagagct cgcgcgtcca cgagtagctc gcttcggcct tcggcatcag gttcgagcgc gacgattcga tatacgacga cgccttcgcg atccgcgcct gcgcctgcgc gatcgatgga ttgccctgga gcgcctcgtc gatcagcttc ggcagctgcg gatcgccgaa ctggctggcc cagtcgagcg tcggccactg gccgccctgg gctggcaggc tttgcgccga ttcgaattgc gacgctggcg cgatctgctt gtcgctcttg atgccgatat agtttgcgca catcgaacca ggaccctggg tctgagcttc cagggtaccc at
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMCANYIGIK SDKQIAPASQ FESAQSLPAQ GGQWPTLDWA SQFGDPQLPK LIDEALQGNP SIAQAQARIA KASSYIESSR SNLMPKAEAS YSWTRELYSS NALFPPPYGG QWYSENNVLA SASWDLDLWG KNRERLHTAV SQEKAAEADM QQARITLASS VARTYNSLAQ LYALRDIAQR EITNRQTVGK ITDGRVSAGL DTNVERQTAR GNIATTQASL SDLDGQITTV RYQLAALLGK GPDRGLQIAA PVMNPSGTVA LPGNLPADLV ARRPDIVAAR WQVEAAMHDV KEAKAEFFPD VNLAAGFGFD AFGWGKFLNF ASRQAQFGPA IHLPIFDAGA LRAQLKGRYA DFDLSVANYN QTLIGALNDV ATQVASIRAV DRQMDDAQRA LDASTRAYDL AVIRYKAGLS PQLQVLTADS NRLASEQTVT NLKMRRRDMQ LALIKALGGG FDATGTRLAA PDAEQQKTQT KQAAN
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GTGCGCAAAC TATATCGGCA TCAAGAGCGA CAAGCAGATC GCGCCAGCGT CGCAATTCGA ATCGGCGCAA AGCCTGCCAG CCCAGGGCGG CCAGTGGCCG ACGCTCGACT GGGCCAGCCA GTTCGGCGAT CCGCAGCTGC CGAAGCTGAT CGACGAGGCG CTCCAGGGCA ATCCATCGAT CGCGCAGGCG CAGGCGCGGA TCGCGAAGGC GTCGTCGTAT ATCGAATCGT CGCGCTCGAA CCTGATGCCG AAGGCCGAAG CGAGCTACTC GTGGACGCGC GAGCTCTATT CTTCCAATGC GCTGTTCCCG CCCCCGTACG GCGGCCAGTG GTACAGCGAG AACAATGTGC TCGCGAGCGC GTCGTGGGAT CTCGACCTGT GGGGCAAGAA TCGCGAGCGG CTGCACACCG CCGTGTCGCA GGAAAAGGCC GCCGAAGCGG ACATGCAGCA GGCGCGCATC ACGCTCGCGT CGTCGGTCGC CCGCACCTAT AACTCGCTCG CGCAACTGTA TGCGCTGCGC GACATCGCGC AGCGCGAGAT CACCAACCGT CAGACGGTCG GCAAGATCAC CGACGGTCGC GTGTCGGCCG GCCTCGACAC TAATGTCGAG CGTCAGACCG CCCGCGGCAA CATCGCGACG ACGCAGGCGT CGCTGTCCGA CCTCGACGGC CAGATCACGA CCGTGCGCTA CCAGCTCGCC GCGCTGCTCG GCAAGGGGCC GGACCGCGGG CTGCAGATCG CCGCGCCGGT GATGAACCCG TCCGGCACGG TCGCCCTGCC CGGCAACCTG CCGGCCGACC TCGTGGCGCG CCGCCCCGAC ATCGTCGCCG CGCGCTGGCA GGTGGAAGCC GCGATGCACG ACGTGAAGGA GGCGAAGGCC GAGTTCTTCC CCGACGTGAA CCTCGCGGCC GGCTTCGGCT TCGACGCGTT CGGCTGGGGC AAATTCCTGA ACTTCGCGAG CCGCCAGGCG CAGTTCGGCC CGGCCATCCA CCTGCCGATC TTCGATGCCG GCGCATTGCG TGCGCAGCTC AAGGGCCGCT ATGCGGACTT CGACCTGTCG GTCGCGAACT ACAACCAGAC GCTGATCGGC GCGCTGAACG ACGTGGCGAC GCAGGTCGCG TCGATCCGCG CGGTCGACCG CCAGATGGAC GACGCGCAGC GTGCGCTCGA CGCATCGACG CGCGCCTACG ACCTGGCCGT GATCCGCTAC AAGGCCGGCC TGTCGCCGCA GCTGCAGGTG CTCACCGCCG ACAGCAACCG CCTCGCATCG GAACAGACGG TCACGAACCT GAAGATGCGC CGGCGCGACA TGCAGCTCGC GCTGATCAAG GCGCTCGGCG GCGGCTTCGA CGCAACCGGC ACGCGGCTCG CGGCGCCCGA CGCAGAACAA CAGAAAACGC AAACAAAGCA GGCCGCCAAC TAAACAGCAC GAACAAGTTC TGCAGCCAAG CTTCTCGAGG ATCCGGCTGC TAACAAAGCC CGAAAGGAAG CTGAGTTGGC TGCTGCCACC GCTGAGCAAT AACTAGCATA ACCCCTTGGG GCCTCTAAAC GGGTCTTGAG GGGTTTTTTG CTGAAAGGAG GAACTATATC CGGATATCCA CAGGACGGGT GTGGTCGCCA TGATCGCGTA GTCGATAGTG GCTCCAAGTA GCGAAGCGAG CAGGACTGGG CGGCGGCCAA AGCGGTCGGA CAGTGCTCCG AGAACGGGTG CGCATAGAAA TTGCATCAAC GCATATAGCG CTAGCAGCAC GCCATAGTGA CTGGCGATGC TGTCGGAATG GACGATATCC CGCAAGAGGC CCGGCAGTAC CGGCATAACC AAGCCTATGC CTACAGCATC CAGGGTGACG GTGCCGAGGA TGACGATGAG CGCATTGTTA GATTTCATAC ACGGTGCCTG ACTGCGTTAG CAATTTAACT GTGATAAACT ACCGCATTAA AGCTTATCGA TGATAAGCTG TCAAACATGA GAA