BuviA.01666.b

Signal transduction histidine kinase, nitrogen specific, NtrB

CENTER ID: BuviA.01666.b
ORGANISM: Burkholderia vietnamiensis G4
ASSOCIATED DISEASE:
CURRENT STATUS: purified
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BuviA.01666.b.A1.GE34869 Full length( BuviA.01666.b ) 1 380
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BuviA.01666.b.A1.PW35484 Full length( BuviA.01666.b ) 1 380
BuviA.01666.b.A1.PW36931 Full length( BuviA.01666.b ) 1 380

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|269482.11.peg.6466
RefSeq: YP_001120064.1
UniProt: A4JG26

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MALKNLIKAK TGQPERLTED ERLARSGLLA GLEALPTVVL VLDRKTLRVA FANPSAEAML DISRRQLAQR PWGEIFPNAN ELASTITAIG EERFHATHLD TVLDRPGHEA LHVHAIVGFL ETAPDFVLVE LFENERQSRT DREERIHDLT AVNKQLIRNL AHEIKNPLGG IRGAAQLLEF ELGERERGEL REYTQVIIKE SDRLQTLVDR LLEPHRHPHI VGDVNIHEVC ELVRAVMLAE FPRGLTIERD YDVSVPDLRG DKEQLIQALL NIVRNAAQAL RERIAQGDAR IELRTRIARK VTIAKRLYRL ALDLHVVDNG PGIPEEIRDR IFYPLVSGRD DGSGLGLTLA QTFVQQHDGM IEVESRPGRT EFQILLPLDH
NT Sequence
ATGGCTCTGA AGAATCTGAT CAAGGCGAAG ACGGGGCAGC CCGAGCGACT GACGGAAGAC GAGCGGCTCG CGCGCTCCGG GCTGCTTGCC GGGCTCGAAG CGCTGCCGAC GGTCGTGCTC GTGCTCGACC GCAAGACGCT GCGGGTCGCG TTCGCGAACC CGTCCGCGGA GGCGATGCTC GACATCTCGC GCCGGCAGCT CGCGCAGCGG CCGTGGGGCG AGATCTTCCC GAACGCGAAC GAGCTCGCGT CGACGATCAC CGCGATCGGC GAGGAGCGCT TTCACGCGAC GCATCTCGAC ACCGTGCTCG ATCGGCCCGG CCACGAGGCG TTGCACGTGC ATGCGATCGT CGGCTTCCTC GAGACCGCGC CCGATTTCGT GCTCGTCGAG CTGTTCGAGA ACGAACGCCA GTCGCGCACC GATCGCGAGG AGCGCATCCA CGACCTGACC GCGGTCAACA AGCAGCTGAT CCGCAATCTC GCGCACGAGA TCAAGAATCC GCTCGGCGGC ATTCGCGGCG CCGCGCAGCT GCTCGAATTC GAGCTGGGCG AGCGCGAGCG TGGCGAGTTG CGCGAATACA CGCAGGTGAT CATCAAGGAG TCCGACCGCC TGCAGACGCT CGTCGACCGG TTGCTGGAGC CGCATCGGCA TCCGCACATC GTCGGCGACG TGAACATTCA TGAAGTATGC GAACTCGTGC GCGCGGTGAT GCTCGCGGAG TTTCCGCGCG GCCTCACGAT CGAGCGCGAC TACGACGTGA GCGTGCCGGA CCTGCGCGGC GACAAGGAGC AGCTGATCCA GGCGCTGCTG AACATCGTGC GCAATGCGGC GCAGGCGCTG CGCGAACGCA TCGCGCAGGG CGACGCGAGA ATCGAATTGC GCACGCGCAT CGCGCGCAAG GTGACGATCG CGAAGCGCCT GTACCGGCTG GCACTGGACT TGCACGTGGT CGACAACGGG CCGGGCATTC CGGAGGAGAT TCGCGACCGC ATCTTCTACC CGCTCGTGTC CGGGCGCGAC GACGGCAGCG GGCTCGGCCT CACGCTCGCG CAAACCTTCG TGCAGCAGCA CGACGGGATG ATCGAGGTCG AGAGCCGGCC CGGACGTACC GAATTTCAGA TCCTGTTGCC GCTCGATCAC
Details for BuviA.01666.b.A1.GE34869
HARVESTED ON: 4/17/2012
SEQUENCED ON: 4/17/2012
EXPECTED MW: 45kDa
OBSERVED MW: 43kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 100
PERCENT COVERAGE: 94
Validated AA Sequence
MALKNLIKAK TGQPERLTED ERLARSGLLA GLEALPTVVL VLDRKTLRVA FANPSAEAML DISRRQLAQR PWGEIFPNAN ELASTITAIG EERFHATHLD TVLDRPGHEA LHVHAIVGFL ETAPDFVLVE LFENERQSRT DREERIHDLT AVNKQLIRNL AHEIKNPLGG IRGAAQLLEF ELGERERGEL REYTQVIIKE SDRLQTLVDR LLEPHRHPHI VGDVNIHEVC ELVRAVMLAE FPRGLTIERD YDVSVPDLRG DKEQLIQALL NIVRNAAQAL RERIAQGDAR IELRTRIARK VTIAKRLYRL ALDLHVVDNG PGIPEEIRDR IFYPLVSGRD DGSGLGLTLA QTFVQQHDGM IEVESRPGRT EFQILLPL
Validated NT Sequence
cgagcggcaa caggatctga aattcggtac gtccgggccg gctctcgacc tcgatcatcc cgtcgtgctg ctgcacgaag gtttgcgcga gcgtgaggcc gagcccgctg ccgtcgtcgc gcccggacac gagcgggtag aagatgcggt cgcgaatctc ctccggaatg cccggcccgt tgtcgaccac gtgcaagtcc agtgccagcc ggtacaggcg cttcgcgatc gtcaccttgc gcgcgatgcg cgtgcgcaat tcgattctcg cgtcgccctg cgcgatgcgt tcgcgcagcg cctgcgccgc attgcgcacg atgttcagca gcgcctggat cagctgctcc ttgtcgccgc gcaggtccgg cacgctcacg tcgtagtcgc gctcgatcgt gaggccgcgc ggaaactccg cgagcatcac cgcgcgcacg agttcgcata cttcatgaat gttcacgtcg ccgacgatgt gcggatgccg atgcggctcc agcaaccggt cgacgagcgt ctgcaggcgg tcggactcct tgatgatcac ctgcgtgtat tcgcgcaact cgccacgctc gcgctcgccc agctcgaatt cgagcagctg cgcggcgccg cgaatgccgc cgagcggatt cttgatctcg tgcgcgagat tgcggatcag ctgcttgttg accgcggtca ggtcgtggat gcgctcctcg cgatcggtgc gcgactggcg ttcgttctcg aacagctcga cgagcacgaa atcgggcgcg gtctcgagga agccgacgat cgcatgcacg tgcaacgcct cgtggccggg ccgatcgagc acggtgtcga gatgcgtcgc gtgaaagcgc tcctcgccga tcgcggtgat cgtcgacgcg agctcgttcg cgttcgggaa gatctcgccc cacggccgct gcgcgagctg ccggcgcgag atgtcgagca tcgcctccgc ggacgggttc gcgaacgcga cccgcagcgt cttgcggtcg agcacgagca cgaccgtcgg cagcgcttcg agcccggcaa gcagcccgga gcgcgcgagc cgctcgtctt ccgtcagtcg ctcgggctgc cccgtcttcg ccttgatcag attcttcaga gccatcgaac caggaccctg ggtctgagct tccag
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMALKNLIKA KTGQPERLTE DERLARSGLL AGLEALPTVV LVLDRKTLRV AFANPSAEAM LDISRRQLAQ RPWGEIFPNA NELASTITAI GEERFHATHL DTVLDRPGHE ALHVHAIVGF LETAPDFVLV ELFENERQSR TDREERIHDL TAVNKQLIRN LAHEIKNPLG GIRGAAQLLE FELGERERGE LREYTQVIIK ESDRLQTLVD RLLEPHRHPH IVGDVNIHEV CELVRAVMLA EFPRGLTIER DYDVSVPDLR GDKEQLIQAL LNIVRNAAQA LRERIAQGDA RIELRTRIAR KVTIAKRLYR LALDLHVVDN GPGIPEEIRD RIFYPLVSGR DDGSGLGLTL AQTFVQQHDG MIEVESRPGR TEFQILLPLD H
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggctctgaag aatctgatca aggcgaagac ggggcagccc gagcgactga cggaagacga gcggctcgcg cgctccgggc tgcttgccgg gctcgaagcg ctgccgacgg tcgtgctcgt gctcgaccgc aagacgctgc gggtcgcgtt cgcgaacccg tccgcggagg cgatgctcga catctcgcgc cggcagctcg cgcagcggcc gtggggcgag atcttcccga acgcgaacga gctcgcgtcg acgatcaccg cgatcggcga ggagcgcttt cacgcgacgc atctcgacac cgtgctcgat cggcccggcc acgaggcgtt gcacgtgcat gcgatcgtcg gcttcctcga gaccgcgccc gatttcgtgc tcgtcgagct gttcgagaac gaacgccagt cgcgcaccga tcgcgaggag cgcatccacg acctgaccgc ggtcaacaag cagctgatcc gcaatctcgc gcacgagatc aagaatccgc tcggcggcat tcgcggcgcc gcgcagctgc tcgaattcga gctgggcgag cgcgagcgtg gcgagttgcg cgaatacacg caggtgatca tcaaggagtc cgaccgcctg cagacgctcg tcgaccggtt gctggagccg catcggcatc cgcacatcgt cggcgacgtg aacattcatg aagtatgcga actcgtgcgc gcggtgatgc tcgcggagtt tccgcgcggc ctcacgatcg agcgcgacta cgacgtgagc gtgccggacc tgcgcggcga caaggagcag ctgatccagg cgctgctgaa catcgtgcgc aatgcggcgc aggcgctgcg cgaacgcatc gcgcagggcg acgcgagaat cgaattgcgc acgcgcatcg cgcgcaaggt gacgatcgcg aagcgcctgt accggctggc actggacttg cacgtggtcg acaacgggcc gggcattccg gaggagattc gcgaccgcat cttctacccg ctcgtgtccg ggcgcgacga cggcagcggg ctcggcctca cgctcgcgca aaccttcgtg cagcagcacg acgggatgat cgaggtcgag agccggcccg gacgtaccga atttcagatc ctgttgccgc tcgatcacta aacagcacga acaagttctg cagccaagct tctcgaggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga a
Details for BuviA.01666.b.A1.PW35484
PURIFICATION DATe: 9/4/2012
CONCENTRATION: 4.93mg/ml
OBSERVED MW: 41kDa
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 7
VIAL VOLUME: 110µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 94
Protocol Notes
notes unavailable
Validated AA Sequence
MALKNLIKAK TGQPERLTED ERLARSGLLA GLEALPTVVL VLDRKTLRVA FANPSAEAML DISRRQLAQR PWGEIFPNAN ELASTITAIG EERFHATHLD TVLDRPGHEA LHVHAIVGFL ETAPDFVLVE LFENERQSRT DREERIHDLT AVNKQLIRNL AHEIKNPLGG IRGAAQLLEF ELGERERGEL REYTQVIIKE SDRLQTLVDR LLEPHRHPHI VGDVNIHEVC ELVRAVMLAE FPRGLTIERD YDVSVPDLRG DKEQLIQALL NIVRNAAQAL RERIAQGDAR IELRTRIARK VTIAKRLYRL ALDLHVVDNG PGIPEEIRDR IFYPLVSGRD DGSGLGLTLA QTFVQQHDGM IEVESRPGRT EFQILLPL
Validated NT Sequence
cgagcggcaa caggatctga aattcggtac gtccgggccg gctctcgacc tcgatcatcc cgtcgtgctg ctgcacgaag gtttgcgcga gcgtgaggcc gagcccgctg ccgtcgtcgc gcccggacac gagcgggtag aagatgcggt cgcgaatctc ctccggaatg cccggcccgt tgtcgaccac gtgcaagtcc agtgccagcc ggtacaggcg cttcgcgatc gtcaccttgc gcgcgatgcg cgtgcgcaat tcgattctcg cgtcgccctg cgcgatgcgt tcgcgcagcg cctgcgccgc attgcgcacg atgttcagca gcgcctggat cagctgctcc ttgtcgccgc gcaggtccgg cacgctcacg tcgtagtcgc gctcgatcgt gaggccgcgc ggaaactccg cgagcatcac cgcgcgcacg agttcgcata cttcatgaat gttcacgtcg ccgacgatgt gcggatgccg atgcggctcc agcaaccggt cgacgagcgt ctgcaggcgg tcggactcct tgatgatcac ctgcgtgtat tcgcgcaact cgccacgctc gcgctcgccc agctcgaatt cgagcagctg cgcggcgccg cgaatgccgc cgagcggatt cttgatctcg tgcgcgagat tgcggatcag ctgcttgttg accgcggtca ggtcgtggat gcgctcctcg cgatcggtgc gcgactggcg ttcgttctcg aacagctcga cgagcacgaa atcgggcgcg gtctcgagga agccgacgat cgcatgcacg tgcaacgcct cgtggccggg ccgatcgagc acggtgtcga gatgcgtcgc gtgaaagcgc tcctcgccga tcgcggtgat cgtcgacgcg agctcgttcg cgttcgggaa gatctcgccc cacggccgct gcgcgagctg ccggcgcgag atgtcgagca tcgcctccgc ggacgggttc gcgaacgcga cccgcagcgt cttgcggtcg agcacgagca cgaccgtcgg cagcgcttcg agcccggcaa gcagcccgga gcgcgcgagc cgctcgtctt ccgtcagtcg ctcgggctgc cccgtcttcg ccttgatcag attcttcaga gccatcgaac caggaccctg ggtctgagct tccag
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMALKNLIKA KTGQPERLTE DERLARSGLL AGLEALPTVV LVLDRKTLRV AFANPSAEAM LDISRRQLAQ RPWGEIFPNA NELASTITAI GEERFHATHL DTVLDRPGHE ALHVHAIVGF LETAPDFVLV ELFENERQSR TDREERIHDL TAVNKQLIRN LAHEIKNPLG GIRGAAQLLE FELGERERGE LREYTQVIIK ESDRLQTLVD RLLEPHRHPH IVGDVNIHEV CELVRAVMLA EFPRGLTIER DYDVSVPDLR GDKEQLIQAL LNIVRNAAQA LRERIAQGDA RIELRTRIAR KVTIAKRLYR LALDLHVVDN GPGIPEEIRD RIFYPLVSGR DDGSGLGLTL AQTFVQQHDG MIEVESRPGR TEFQILLPLD H
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGCTCTGAAG AATCTGATCA AGGCGAAGAC GGGGCAGCCC GAGCGACTGA CGGAAGACGA GCGGCTCGCG CGCTCCGGGC TGCTTGCCGG GCTCGAAGCG CTGCCGACGG TCGTGCTCGT GCTCGACCGC AAGACGCTGC GGGTCGCGTT CGCGAACCCG TCCGCGGAGG CGATGCTCGA CATCTCGCGC CGGCAGCTCG CGCAGCGGCC GTGGGGCGAG ATCTTCCCGA ACGCGAACGA GCTCGCGTCG ACGATCACCG CGATCGGCGA GGAGCGCTTT CACGCGACGC ATCTCGACAC CGTGCTCGAT CGGCCCGGCC ACGAGGCGTT GCACGTGCAT GCGATCGTCG GCTTCCTCGA GACCGCGCCC GATTTCGTGC TCGTCGAGCT GTTCGAGAAC GAACGCCAGT CGCGCACCGA TCGCGAGGAG CGCATCCACG ACCTGACCGC GGTCAACAAG CAGCTGATCC GCAATCTCGC GCACGAGATC AAGAATCCGC TCGGCGGCAT TCGCGGCGCC GCGCAGCTGC TCGAATTCGA GCTGGGCGAG CGCGAGCGTG GCGAGTTGCG CGAATACACG CAGGTGATCA TCAAGGAGTC CGACCGCCTG CAGACGCTCG TCGACCGGTT GCTGGAGCCG CATCGGCATC CGCACATCGT CGGCGACGTG AACATTCATG AAGTATGCGA ACTCGTGCGC GCGGTGATGC TCGCGGAGTT TCCGCGCGGC CTCACGATCG AGCGCGACTA CGACGTGAGC GTGCCGGACC TGCGCGGCGA CAAGGAGCAG CTGATCCAGG CGCTGCTGAA CATCGTGCGC AATGCGGCGC AGGCGCTGCG CGAACGCATC GCGCAGGGCG ACGCGAGAAT CGAATTGCGC ACGCGCATCG CGCGCAAGGT GACGATCGCG AAGCGCCTGT ACCGGCTGGC ACTGGACTTG CACGTGGTCG ACAACGGGCC GGGCATTCCG GAGGAGATTC GCGACCGCAT CTTCTACCCG CTCGTGTCCG GGCGCGACGA CGGCAGCGGG CTCGGCCTCA CGCTCGCGCA AACCTTCGTG CAGCAGCACG ACGGGATGAT CGAGGTCGAG AGCCGGCCCG GACGTACCGA ATTTCAGATC CTGTTGCCGC TCGATCACTA AACAGCACGA ACAAGTTCTG CAGCCAAGCT TCTCGAGGAT CCGGCTGCTA ACAAAGCCCG AAAGGAAGCT GAGTTGGCTG CTGCCACCGC TGAGCAATAA CTAGCATAAC CCCTTGGGGC CTCTAAACGG GTCTTGAGGG GTTTTTTGCT GAAAGGAGGA ACTATATCCG GATATCCACA GGACGGGTGT GGTCGCCATG ATCGCGTAGT CGATAGTGGC TCCAAGTAGC GAAGCGAGCA GGACTGGGCG GCGGCCAAAG CGGTCGGACA GTGCTCCGAG AACGGGTGCG CATAGAAATT GCATCAACGC ATATAGCGCT AGCAGCACGC CATAGTGACT GGCGATGCTG TCGGAATGGA CGATATCCCG CAAGAGGCCC GGCAGTACCG GCATAACCAA GCCTATGCCT ACAGCATCCA GGGTGACGGT GCCGAGGATG ACGATGAGCG CATTGTTAGA TTTCATACAC GGTGCCTGAC TGCGTTAGCA ATTTAACTGT GATAAACTAC CGCATTAAAG CTTATCGATG ATAAGCTGTC AAACATGAGA A
Details for BuviA.01666.b.A1.PW36931
PURIFICATION DATe: 9/4/2012
CONCENTRATION: 15.8mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 5
VIAL VOLUME: 110µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 94
Protocol Notes
notes unavailable
Validated AA Sequence
MALKNLIKAK TGQPERLTED ERLARSGLLA GLEALPTVVL VLDRKTLRVA FANPSAEAML DISRRQLAQR PWGEIFPNAN ELASTITAIG EERFHATHLD TVLDRPGHEA LHVHAIVGFL ETAPDFVLVE LFENERQSRT DREERIHDLT AVNKQLIRNL AHEIKNPLGG IRGAAQLLEF ELGERERGEL REYTQVIIKE SDRLQTLVDR LLEPHRHPHI VGDVNIHEVC ELVRAVMLAE FPRGLTIERD YDVSVPDLRG DKEQLIQALL NIVRNAAQAL RERIAQGDAR IELRTRIARK VTIAKRLYRL ALDLHVVDNG PGIPEEIRDR IFYPLVSGRD DGSGLGLTLA QTFVQQHDGM IEVESRPGRT EFQILLPL
Validated NT Sequence
cgagcggcaa caggatctga aattcggtac gtccgggccg gctctcgacc tcgatcatcc cgtcgtgctg ctgcacgaag gtttgcgcga gcgtgaggcc gagcccgctg ccgtcgtcgc gcccggacac gagcgggtag aagatgcggt cgcgaatctc ctccggaatg cccggcccgt tgtcgaccac gtgcaagtcc agtgccagcc ggtacaggcg cttcgcgatc gtcaccttgc gcgcgatgcg cgtgcgcaat tcgattctcg cgtcgccctg cgcgatgcgt tcgcgcagcg cctgcgccgc attgcgcacg atgttcagca gcgcctggat cagctgctcc ttgtcgccgc gcaggtccgg cacgctcacg tcgtagtcgc gctcgatcgt gaggccgcgc ggaaactccg cgagcatcac cgcgcgcacg agttcgcata cttcatgaat gttcacgtcg ccgacgatgt gcggatgccg atgcggctcc agcaaccggt cgacgagcgt ctgcaggcgg tcggactcct tgatgatcac ctgcgtgtat tcgcgcaact cgccacgctc gcgctcgccc agctcgaatt cgagcagctg cgcggcgccg cgaatgccgc cgagcggatt cttgatctcg tgcgcgagat tgcggatcag ctgcttgttg accgcggtca ggtcgtggat gcgctcctcg cgatcggtgc gcgactggcg ttcgttctcg aacagctcga cgagcacgaa atcgggcgcg gtctcgagga agccgacgat cgcatgcacg tgcaacgcct cgtggccggg ccgatcgagc acggtgtcga gatgcgtcgc gtgaaagcgc tcctcgccga tcgcggtgat cgtcgacgcg agctcgttcg cgttcgggaa gatctcgccc cacggccgct gcgcgagctg ccggcgcgag atgtcgagca tcgcctccgc ggacgggttc gcgaacgcga cccgcagcgt cttgcggtcg agcacgagca cgaccgtcgg cagcgcttcg agcccggcaa gcagcccgga gcgcgcgagc cgctcgtctt ccgtcagtcg ctcgggctgc cccgtcttcg ccttgatcag attcttcaga gccatcgaac caggaccctg ggtctgagct tccag
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMALKNLIKA KTGQPERLTE DERLARSGLL AGLEALPTVV LVLDRKTLRV AFANPSAEAM LDISRRQLAQ RPWGEIFPNA NELASTITAI GEERFHATHL DTVLDRPGHE ALHVHAIVGF LETAPDFVLV ELFENERQSR TDREERIHDL TAVNKQLIRN LAHEIKNPLG GIRGAAQLLE FELGERERGE LREYTQVIIK ESDRLQTLVD RLLEPHRHPH IVGDVNIHEV CELVRAVMLA EFPRGLTIER DYDVSVPDLR GDKEQLIQAL LNIVRNAAQA LRERIAQGDA RIELRTRIAR KVTIAKRLYR LALDLHVVDN GPGIPEEIRD RIFYPLVSGR DDGSGLGLTL AQTFVQQHDG MIEVESRPGR TEFQILLPLD H
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGCTCTGAAG AATCTGATCA AGGCGAAGAC GGGGCAGCCC GAGCGACTGA CGGAAGACGA GCGGCTCGCG CGCTCCGGGC TGCTTGCCGG GCTCGAAGCG CTGCCGACGG TCGTGCTCGT GCTCGACCGC AAGACGCTGC GGGTCGCGTT CGCGAACCCG TCCGCGGAGG CGATGCTCGA CATCTCGCGC CGGCAGCTCG CGCAGCGGCC GTGGGGCGAG ATCTTCCCGA ACGCGAACGA GCTCGCGTCG ACGATCACCG CGATCGGCGA GGAGCGCTTT CACGCGACGC ATCTCGACAC CGTGCTCGAT CGGCCCGGCC ACGAGGCGTT GCACGTGCAT GCGATCGTCG GCTTCCTCGA GACCGCGCCC GATTTCGTGC TCGTCGAGCT GTTCGAGAAC GAACGCCAGT CGCGCACCGA TCGCGAGGAG CGCATCCACG ACCTGACCGC GGTCAACAAG CAGCTGATCC GCAATCTCGC GCACGAGATC AAGAATCCGC TCGGCGGCAT TCGCGGCGCC GCGCAGCTGC TCGAATTCGA GCTGGGCGAG CGCGAGCGTG GCGAGTTGCG CGAATACACG CAGGTGATCA TCAAGGAGTC CGACCGCCTG CAGACGCTCG TCGACCGGTT GCTGGAGCCG CATCGGCATC CGCACATCGT CGGCGACGTG AACATTCATG AAGTATGCGA ACTCGTGCGC GCGGTGATGC TCGCGGAGTT TCCGCGCGGC CTCACGATCG AGCGCGACTA CGACGTGAGC GTGCCGGACC TGCGCGGCGA CAAGGAGCAG CTGATCCAGG CGCTGCTGAA CATCGTGCGC AATGCGGCGC AGGCGCTGCG CGAACGCATC GCGCAGGGCG ACGCGAGAAT CGAATTGCGC ACGCGCATCG CGCGCAAGGT GACGATCGCG AAGCGCCTGT ACCGGCTGGC ACTGGACTTG CACGTGGTCG ACAACGGGCC GGGCATTCCG GAGGAGATTC GCGACCGCAT CTTCTACCCG CTCGTGTCCG GGCGCGACGA CGGCAGCGGG CTCGGCCTCA CGCTCGCGCA AACCTTCGTG CAGCAGCACG ACGGGATGAT CGAGGTCGAG AGCCGGCCCG GACGTACCGA ATTTCAGATC CTGTTGCCGC TCGATCACTA AACAGCACGA ACAAGTTCTG CAGCCAAGCT TCTCGAGGAT CCGGCTGCTA ACAAAGCCCG AAAGGAAGCT GAGTTGGCTG CTGCCACCGC TGAGCAATAA CTAGCATAAC CCCTTGGGGC CTCTAAACGG GTCTTGAGGG GTTTTTTGCT GAAAGGAGGA ACTATATCCG GATATCCACA GGACGGGTGT GGTCGCCATG ATCGCGTAGT CGATAGTGGC TCCAAGTAGC GAAGCGAGCA GGACTGGGCG GCGGCCAAAG CGGTCGGACA GTGCTCCGAG AACGGGTGCG CATAGAAATT GCATCAACGC ATATAGCGCT AGCAGCACGC CATAGTGACT GGCGATGCTG TCGGAATGGA CGATATCCCG CAAGAGGCCC GGCAGTACCG GCATAACCAA GCCTATGCCT ACAGCATCCA GGGTGACGGT GCCGAGGATG ACGATGAGCG CATTGTTAGA TTTCATACAC GGTGCCTGAC TGCGTTAGCA ATTTAACTGT GATAAACTAC CGCATTAAAG CTTATCGATG ATAAGCTGTC AAACATGAGA A