BuviA.01664.a

Histidine kinase

CENTER ID: BuviA.01664.a
ORGANISM: Burkholderia vietnamiensis G4
ASSOCIATED DISEASE:
CURRENT STATUS: purified
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BuviA.01664.a.A1.GE34868 Full length( BuviA.01664.a ) 1 373
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BuviA.01664.a.A1.PW35483 Full length( BuviA.01664.a ) 1 373

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|269482.11.peg.1197
RefSeq: YP_001114992.1
UniProt: A4JR34

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MRLAEFITRN MEPILVQWEA FAATLLPAAR TMDSHALRDH ARQILEAIAK DIATPQTREE QREKSLGRAP KPTNASETAA QTHAVLRARR GFDINQLAAE YRALRASVLG MWIDECRPSP PDLDDMIRFN EAIDQALAES VAFFTEQVEQ ARNLLLGMLG HDMRTPLQTI RLTASYLSAL NAGEKVSEAA ARLIRSGGRM QSLLDDLCDF SRTQLGLGIN VIRRDADLAH VVANVVDELR AAHPDREINV DVRGDLRGNW DDQRMQQLLS NLLTNALKYG TANTPVRASA IASDDEVTIE IGNSGPPVAP DLLERIFDPL QRGTSPSEKS GEDVGLGLGL YIASEIAHAH RGRIDARSDA SETVFAVRLP RRA
NT Sequence
ATGCGTCTAG CCGAATTCAT CACGCGCAAC ATGGAACCGA TCCTCGTGCA GTGGGAAGCG TTCGCTGCAA CGCTGCTCCC GGCCGCGAGA ACCATGGACT CCCACGCGCT GCGCGACCAT GCGCGCCAGA TCCTGGAGGC GATTGCCAAG GACATCGCAA CCCCACAAAC GCGGGAAGAA CAGCGGGAAA AATCGCTCGG CCGCGCACCC AAGCCGACGA ATGCCTCGGA AACGGCCGCC CAGACGCATG CCGTGCTGCG CGCGCGGCGC GGCTTCGACA TCAACCAGCT GGCGGCCGAG TATCGCGCGC TGCGCGCCAG CGTGCTGGGG ATGTGGATCG ACGAGTGTCG GCCGAGCCCG CCCGATCTCG ACGACATGAT CCGCTTCAAC GAAGCGATCG ATCAGGCGCT CGCCGAATCG GTCGCGTTCT TTACCGAGCA GGTCGAGCAG GCGCGCAACC TGTTGCTCGG CATGCTCGGG CACGACATGC GCACGCCGTT GCAAACGATA CGCCTGACGG CAAGCTATCT GTCGGCGCTC AATGCGGGCG AAAAGGTGTC GGAAGCCGCC GCCAGGCTGA TCAGAAGCGG CGGTCGCATG CAGTCGCTGC TCGACGACCT GTGTGATTTC AGCCGGACGC AGCTCGGGCT CGGCATCAAC GTGATTCGCC GCGACGCGGA TCTCGCCCAT GTCGTGGCCA ATGTGGTCGA TGAGTTGCGG GCCGCGCATC CGGACCGCGA AATCAATGTC GACGTGCGCG GCGACCTCCG CGGCAACTGG GACGATCAGC GGATGCAGCA GTTGCTCAGC AATCTGTTGA CCAACGCGCT GAAGTACGGA ACGGCCAATA CGCCGGTTCG CGCCAGCGCG ATCGCGAGCG ACGACGAAGT GACGATCGAG ATCGGCAACA GCGGTCCGCC CGTCGCGCCG GATCTGCTGG AGCGCATCTT CGATCCGCTG CAACGCGGCA CCAGTCCATC AGAGAAAAGC GGCGAGGACG TCGGACTGGG CCTCGGCCTG TACATCGCTA GCGAAATCGC CCACGCGCAT CGCGGGCGGA TCGACGCGCG CTCCGACGCG TCGGAAACGG TATTCGCGGT GCGGCTGCCG CGACGCGCG
Details for BuviA.01664.a.A1.GE34868
HARVESTED ON: 4/17/2012
SEQUENCED ON: 4/17/2012
EXPECTED MW: 43kDa
OBSERVED MW: 43kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 100
PERCENT COVERAGE: 98
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMRLAEFITR NMEPILVQWE AFAATLLPAA RTMDSHALRD HARQILEAIA KDIATPQTRE EQREKSLGRA PKPTNASETA AQTHAVLRAR RGFDINQLAA EYRALRASVL GMWIDECRPS PPDLDDMIRF NEAIDQALAE SVAFFTEQVE QARNLLLGML GHDMRTPLQT IRLTASYLSA LNAGEKVSEA AARLIRSGGR MQSLLDDLCD FSRTQLGLGI NVIRRDADLA HVVANVVDEL RAAHPDREIN VDVRGDLRGN WDDQRMQQLL SNLLTNALKY GTANTPVRAS AIASDDEVTI EIGNSGPPVA PDLLERIFDP LQRGTSPSEK SGEDVGLGLG LYIASEIAHA HRGRIDARSD ASETVFAVRL
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgcgtc tagccgaatt catcacgcgc aacatggaac cgatcctcgt gcagtgggaa gcgttcgctg caacgctgct cccggccgcg agaaccatgg actcccacgc gctgcgcgac catgcgcgcc agatcctgga ggcgattgcc aaggacatcg caaccccaca aacgcgggaa gaacagcggg aaaaatcgct cggccgcgca cccaagccga cgaatgcctc ggaaacggcc gcccagacgc atgccgtgct gcgcgcgcgg cgcggcttcg acatcaacca gctggcggcc gagtatcgcg cgctgcgcgc cagcgtgctg gggatgtgga tcgacgagtg tcggccgagc ccgcccgatc tcgacgacat gatccgcttc aacgaagcga tcgatcaggc gctcgccgaa tcggtcgcgt tctttaccga gcaggtcgag caggcgcgca acctgttgct cggcatgctc gggcacgaca tgcgcacgcc gttgcaaacg atacgcctga cggcaagcta tctgtcggcg ctcaatgcgg gcgaaaaggt gtcggaagcc gccgccaggc tgatcagaag cggcggtcgc atgcagtcgc tgctcgacga cctgtgtgat ttcagccgga cgcagctcgg gctcggcatc aacgtgattc gccgcgacgc ggatctcgcc catgtcgtgg ccaatgtggt cgatgagttg cgggccgcgc atccggaccg cgaaatcaat gtcgacgtgc gcggcgacct ccgcggcaac tgggacgatc agcggatgca gcagttgctc agcaatctgt tgaccaacgc gctgaagtac ggaacggcca atacgccggt tcgcgccagc gcgatcgcga gcgacgacga agtgacgatc gagatcggca acagcggtcc gcccgtcgcg ccggatctgc tggagcgcat cttcgatccg ctgcaacgcg gcaccagtcc atcagagaaa agcggcgagg acgtcggact gggcctcggc ctgtacatcg ctagcgaaat cgcccacgcg catcgcgggc ggatcgacgc gcgctccgac gcgtcggaaa cggtattcgc ggtgcggctg ccg
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMRLAEFITR NMEPILVQWE AFAATLLPAA RTMDSHALRD HARQILEAIA KDIATPQTRE EQREKSLGRA PKPTNASETA AQTHAVLRAR RGFDINQLAA EYRALRASVL GMWIDECRPS PPDLDDMIRF NEAIDQALAE SVAFFTEQVE QARNLLLGML GHDMRTPLQT IRLTASYLSA LNAGEKVSEA AARLIRSGGR MQSLLDDLCD FSRTQLGLGI NVIRRDADLA HVVANVVDEL RAAHPDREIN VDVRGDLRGN WDDQRMQQLL SNLLTNALKY GTANTPVRAS AIASDDEVTI EIGNSGPPVA PDLLERIFDP LQRGTSPSEK SGEDVGLGLG LYIASEIAHA HRGRIDARSD ASETVFAVRL PRRA
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gcgtctagcc gaattcatca cgcgcaacat ggaaccgatc ctcgtgcagt gggaagcgtt cgctgcaacg ctgctcccgg ccgcgagaac catggactcc cacgcgctgc gcgaccatgc gcgccagatc ctggaggcga ttgccaagga catcgcaacc ccacaaacgc gggaagaaca gcgggaaaaa tcgctcggcc gcgcacccaa gccgacgaat gcctcggaaa cggccgccca gacgcatgcc gtgctgcgcg cgcggcgcgg cttcgacatc aaccagctgg cggccgagta tcgcgcgctg cgcgccagcg tgctggggat gtggatcgac gagtgtcggc cgagcccgcc cgatctcgac gacatgatcc gcttcaacga agcgatcgat caggcgctcg ccgaatcggt cgcgttcttt accgagcagg tcgagcaggc gcgcaacctg ttgctcggca tgctcgggca cgacatgcgc acgccgttgc aaacgatacg cctgacggca agctatctgt cggcgctcaa tgcgggcgaa aaggtgtcgg aagccgccgc caggctgatc agaagcggcg gtcgcatgca gtcgctgctc gacgacctgt gtgatttcag ccggacgcag ctcgggctcg gcatcaacgt gattcgccgc gacgcggatc tcgcccatgt cgtggccaat gtggtcgatg agttgcgggc cgcgcatccg gaccgcgaaa tcaatgtcga cgtgcgcggc gacctccgcg gcaactggga cgatcagcgg atgcagcagt tgctcagcaa tctgttgacc aacgcgctga agtacggaac ggccaatacg ccggttcgcg ccagcgcgat cgcgagcgac gacgaagtga cgatcgagat cggcaacagc ggtccgcccg tcgcgccgga tctgctggag cgcatcttcg atccgctgca acgcggcacc agtccatcag agaaaagcgg cgaggacgtc ggactgggcc tcggcctgta catcgctagc gaaatcgccc acgcgcatcg cgggcggatc gacgcgcgct ccgacgcgtc ggaaacggta ttcgcggtgc ggctgccgcg acgcgcgtaa acagcacgaa caagttctgc agccaagctt ctcgaggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa
Details for BuviA.01664.a.A1.PW35483
PURIFICATION DATe: 6/21/2012
CONCENTRATION: 16mg/ml
OBSERVED MW: 38kDa
EXPRESSION LEVEL: High Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 18
VIAL VOLUME: 200µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 98
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMRLAEFITR NMEPILVQWE AFAATLLPAA RTMDSHALRD HARQILEAIA KDIATPQTRE EQREKSLGRA PKPTNASETA AQTHAVLRAR RGFDINQLAA EYRALRASVL GMWIDECRPS PPDLDDMIRF NEAIDQALAE SVAFFTEQVE QARNLLLGML GHDMRTPLQT IRLTASYLSA LNAGEKVSEA AARLIRSGGR MQSLLDDLCD FSRTQLGLGI NVIRRDADLA HVVANVVDEL RAAHPDREIN VDVRGDLRGN WDDQRMQQLL SNLLTNALKY GTANTPVRAS AIASDDEVTI EIGNSGPPVA PDLLERIFDP LQRGTSPSEK SGEDVGLGLG LYIASEIAHA HRGRIDARSD ASETVFAVRL
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgcgtc tagccgaatt catcacgcgc aacatggaac cgatcctcgt gcagtgggaa gcgttcgctg caacgctgct cccggccgcg agaaccatgg actcccacgc gctgcgcgac catgcgcgcc agatcctgga ggcgattgcc aaggacatcg caaccccaca aacgcgggaa gaacagcggg aaaaatcgct cggccgcgca cccaagccga cgaatgcctc ggaaacggcc gcccagacgc atgccgtgct gcgcgcgcgg cgcggcttcg acatcaacca gctggcggcc gagtatcgcg cgctgcgcgc cagcgtgctg gggatgtgga tcgacgagtg tcggccgagc ccgcccgatc tcgacgacat gatccgcttc aacgaagcga tcgatcaggc gctcgccgaa tcggtcgcgt tctttaccga gcaggtcgag caggcgcgca acctgttgct cggcatgctc gggcacgaca tgcgcacgcc gttgcaaacg atacgcctga cggcaagcta tctgtcggcg ctcaatgcgg gcgaaaaggt gtcggaagcc gccgccaggc tgatcagaag cggcggtcgc atgcagtcgc tgctcgacga cctgtgtgat ttcagccgga cgcagctcgg gctcggcatc aacgtgattc gccgcgacgc ggatctcgcc catgtcgtgg ccaatgtggt cgatgagttg cgggccgcgc atccggaccg cgaaatcaat gtcgacgtgc gcggcgacct ccgcggcaac tgggacgatc agcggatgca gcagttgctc agcaatctgt tgaccaacgc gctgaagtac ggaacggcca atacgccggt tcgcgccagc gcgatcgcga gcgacgacga agtgacgatc gagatcggca acagcggtcc gcccgtcgcg ccggatctgc tggagcgcat cttcgatccg ctgcaacgcg gcaccagtcc atcagagaaa agcggcgagg acgtcggact gggcctcggc ctgtacatcg ctagcgaaat cgcccacgcg catcgcgggc ggatcgacgc gcgctccgac gcgtcggaaa cggtattcgc ggtgcggctg ccg
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMRLAEFITR NMEPILVQWE AFAATLLPAA RTMDSHALRD HARQILEAIA KDIATPQTRE EQREKSLGRA PKPTNASETA AQTHAVLRAR RGFDINQLAA EYRALRASVL GMWIDECRPS PPDLDDMIRF NEAIDQALAE SVAFFTEQVE QARNLLLGML GHDMRTPLQT IRLTASYLSA LNAGEKVSEA AARLIRSGGR MQSLLDDLCD FSRTQLGLGI NVIRRDADLA HVVANVVDEL RAAHPDREIN VDVRGDLRGN WDDQRMQQLL SNLLTNALKY GTANTPVRAS AIASDDEVTI EIGNSGPPVA PDLLERIFDP LQRGTSPSEK SGEDVGLGLG LYIASEIAHA HRGRIDARSD ASETVFAVRL PRRA
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GCGTCTAGCC GAATTCATCA CGCGCAACAT GGAACCGATC CTCGTGCAGT GGGAAGCGTT CGCTGCAACG CTGCTCCCGG CCGCGAGAAC CATGGACTCC CACGCGCTGC GCGACCATGC GCGCCAGATC CTGGAGGCGA TTGCCAAGGA CATCGCAACC CCACAAACGC GGGAAGAACA GCGGGAAAAA TCGCTCGGCC GCGCACCCAA GCCGACGAAT GCCTCGGAAA CGGCCGCCCA GACGCATGCC GTGCTGCGCG CGCGGCGCGG CTTCGACATC AACCAGCTGG CGGCCGAGTA TCGCGCGCTG CGCGCCAGCG TGCTGGGGAT GTGGATCGAC GAGTGTCGGC CGAGCCCGCC CGATCTCGAC GACATGATCC GCTTCAACGA AGCGATCGAT CAGGCGCTCG CCGAATCGGT CGCGTTCTTT ACCGAGCAGG TCGAGCAGGC GCGCAACCTG TTGCTCGGCA TGCTCGGGCA CGACATGCGC ACGCCGTTGC AAACGATACG CCTGACGGCA AGCTATCTGT CGGCGCTCAA TGCGGGCGAA AAGGTGTCGG AAGCCGCCGC CAGGCTGATC AGAAGCGGCG GTCGCATGCA GTCGCTGCTC GACGACCTGT GTGATTTCAG CCGGACGCAG CTCGGGCTCG GCATCAACGT GATTCGCCGC GACGCGGATC TCGCCCATGT CGTGGCCAAT GTGGTCGATG AGTTGCGGGC CGCGCATCCG GACCGCGAAA TCAATGTCGA CGTGCGCGGC GACCTCCGCG GCAACTGGGA CGATCAGCGG ATGCAGCAGT TGCTCAGCAA TCTGTTGACC AACGCGCTGA AGTACGGAAC GGCCAATACG CCGGTTCGCG CCAGCGCGAT CGCGAGCGAC GACGAAGTGA CGATCGAGAT CGGCAACAGC GGTCCGCCCG TCGCGCCGGA TCTGCTGGAG CGCATCTTCG ATCCGCTGCA ACGCGGCACC AGTCCATCAG AGAAAAGCGG CGAGGACGTC GGACTGGGCC TCGGCCTGTA CATCGCTAGC GAAATCGCCC ACGCGCATCG CGGGCGGATC GACGCGCGCT CCGACGCGTC GGAAACGGTA TTCGCGGTGC GGCTGCCGCG ACGCGCGTAA ACAGCACGAA CAAGTTCTGC AGCCAAGCTT CTCGAGGATC CGGCTGCTAA CAAAGCCCGA AAGGAAGCTG AGTTGGCTGC TGCCACCGCT GAGCAATAAC TAGCATAACC CCTTGGGGCC TCTAAACGGG TCTTGAGGGG TTTTTTGCTG AAAGGAGGAA CTATATCCGG ATATCCACAG GACGGGTGTG GTCGCCATGA TCGCGTAGTC GATAGTGGCT CCAAGTAGCG AAGCGAGCAG GACTGGGCGG CGGCCAAAGC GGTCGGACAG TGCTCCGAGA ACGGGTGCGC ATAGAAATTG CATCAACGCA TATAGCGCTA GCAGCACGCC ATAGTGACTG GCGATGCTGT CGGAATGGAC GATATCCCGC AAGAGGCCCG GCAGTACCGG CATAACCAAG CCTATGCCTA CAGCATCCAG GGTGACGGTG CCGAGGATGA CGATGAGCGC ATTGTTAGAT TTCATACACG GTGCCTGACT GCGTTAGCAA TTTAACTGTG ATAAACTACC GCATTAAAGC TTATCGATGA TAAGCTGTCA AACATGAGAA