BuviA.01136.a

1-deoxy-D-xylulose 5-phosphate reductoisomerase (IspC)

CENTER ID:	BuviA.01136.a
ORGANISM:	Burkholderia vietnamiensis G4
ASSOCIATED DISEASE:
CURRENT STATUS:	purified
COMMUNITY REQUEST:	True
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BuviA.01136.a.A1.GE34871	Full length( BuviA.01136.a )		1	398

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BuviA.01136.a.A1.PS01530	Full length( BuviA.01136.a )		1	398	unavailable

External Resources

RESOURCE	REFERENCE ID
OrthoMCL:	OG5_130462
PATRIC ID:	fig\|269482.11.peg.6182
RefSeq:	YP_001119757.1
UniProt:	A4JF69

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MQKRLTLLGS TGSIGDSTLD VVARHPERFS VYALTAHRNG DKLVEQCLRF APEVAVVGDA ATAAHVEAKL RAAGSKTTVL YGPQALVDVS ASDGCDTVVA AIVGAAGLAP SLAAARAGKR ILLANKESLV MSGGIFMDAV RDHGAILLPV DSEHNAIFQC MPRDENEHGG ISKIILTASG GPFRTREPAT LADVTPDEAC KHPNWVMGRK ISVDSATMMN KGLEVIEAHW IFGLPGERID VLIHPQSVIH SLVSYRDGSV LAQLGNPDMR TPIAHALAFP ERVDAGVDQL DLAQIAQLSF EKPDYARFPC LALALKALEE GGIASAALNA ANEVAVEAFL ERRIGFMAIA ATVDAVLNAL PNRSPNGLDD VLAADADARR LAAGFIAKAP APRVERIV

NT Sequence

ATGCAAAAAC GTCTGACACT GCTCGGTTCC ACGGGCTCGA TCGGAGACAG CACGCTCGAC GTGGTCGCCC GCCATCCCGA GCGTTTTTCG GTGTACGCGC TGACCGCGCA TCGCAACGGC GACAAGCTCG TCGAGCAATG CCTGCGCTTC GCGCCGGAAG TCGCGGTGGT CGGCGACGCG GCGACCGCCG CGCACGTCGA AGCAAAGCTG CGCGCCGCGG GCAGCAAGAC CACGGTGCTG TATGGTCCGC AGGCGCTCGT CGACGTGTCG GCGAGCGACG GCTGCGACAC GGTCGTCGCG GCGATCGTCG GCGCGGCCGG CCTCGCGCCG AGCCTCGCGG CCGCGCGCGC GGGCAAGCGC ATCCTGCTCG CGAACAAGGA ATCGCTGGTG ATGTCGGGCG GGATCTTCAT GGACGCGGTG CGCGACCATG GCGCGATCCT GCTGCCGGTC GACAGCGAGC ACAACGCGAT CTTCCAGTGC ATGCCGCGCG ACGAGAACGA GCACGGCGGC ATCTCGAAGA TCATCCTGAC GGCGTCGGGC GGCCCGTTCC GCACGCGCGA GCCGGCCACG CTCGCCGACG TGACGCCGGA CGAGGCGTGC AAGCATCCGA ACTGGGTGAT GGGCCGCAAG ATCTCCGTCG ATTCGGCGAC GATGATGAAC AAGGGCCTCG AGGTGATCGA GGCGCACTGG ATCTTCGGTC TGCCGGGCGA GCGGATCGAC GTGCTGATCC ACCCGCAGAG CGTGATCCAT TCGCTGGTGT CGTATCGCGA CGGGTCGGTG CTCGCGCAGC TCGGCAACCC CGACATGCGT ACGCCGATCG CGCACGCGCT CGCGTTCCCG GAGCGCGTCG ACGCCGGCGT CGATCAGCTC GACCTCGCGC AGATCGCGCA GCTGTCGTTC GAGAAGCCGG ATTACGCACG CTTCCCGTGT CTCGCGCTCG CGCTGAAGGC GCTTGAAGAG GGCGGGATCG CGAGCGCCGC ATTGAACGCG GCGAACGAAG TCGCGGTCGA AGCGTTTCTC GAGCGCCGGA TCGGCTTCAT GGCGATCGCG GCGACGGTCG ACGCGGTGCT CAACGCACTG CCGAACCGCA GCCCGAACGG GCTCGACGAC GTCCTGGCGG CCGATGCCGA CGCGCGTCGT CTTGCCGCCG GGTTCATTGC AAAAGCGCCT GCGCCTCGCG TGGAGCGTAT CGTC

Details for BuviA.01136.a.A1.GE34871

HARVESTED ON:	4/17/2012
SEQUENCED ON:	4/17/2012
EXPECTED MW:	44kDa
OBSERVED MW:	44kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	High Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	99
PERCENT COVERAGE:	63

Validated AA Sequence

MSGGIFMDAV RDHGAXXLPV DSEHNAIFQC MPRDENEHGG ISKIILTASG GPFRTREPAT LADVTPDEAC KHPNWVMGRK ISVDSATMMN KGLEVIEAHW IFGLPGERID VLIHPQSVIH SLVSYRDGSV LAQLGNPDMR TPIAHALAFP ERVDAGVDQL DLAQIAQLSF EKPDYARFPC LALALKALEE GGIASAALNA ANEVAVEAFL ERRIGFMAIA ATVDAVLNAL PNRSPNGLDD VLAADADARR LAAGFIAKAP APRV

Validated NT Sequence

ctccacgcga ggcgcaggcg cttttgcaat gaacccggcg gcaagacgac gcgcgtcggc atcggccgcc aggacgtcgt cgagcccgtt cgggctgcgg ttcggcagtg cgttgagcac cgcgtcgacc gtcgccgcga tcgccatgaa gccgatccgg cgctcgagaa acgcttcgac cgcgacttcg ttcgccgcgt tcaatgcggc gctcgcgatc ccgccctctt caagcgcctt cagcgcgagc gcgagacacg ggaagcgtgc gtaatccggc ttctcgaacg acagctgcgc gatctgcgcg aggtcgagct gatcgacgcc ggcgtcgacg cgctccggga acgcgagcgc gtgcgcgatc ggcgtacgca tgtcggggtt gccgagctgc gcgagcaccg acccgtcgcg atacgacacc agcgaatgga tcacgctctg cgggtggatc agcacgtcga tccgctcgcc cggcagaccg aagatccagt gcgcctcgat cacctcgagg cccttgttca tcatcgtcgc cgaatcgacg gagatcttgc ggcccatcac ccagttcgga tgcttgcacg cctcgtccgg cgtcacgtcg gcgagcgtgg ccggctcgcg cgtgcggaac gggccgcccg acgccgtcag gatgatcttc gagatgccgc cgtgctcgtt ctcgtcgcgc ggcatgcact ggaagatcgc gttgtgctcg ctgtcgaccg gcagcnnnat cgcgccatgg tcgcgcaccg cgtccatgaa gatcccgccc gacatcacca gcgattcc

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMQKRLTLLG STGSIGDSTL DVVARHPERF SVYALTAHRN GDKLVEQCLR FAPEVAVVGD AATAAHVEAK LRAAGSKTTV LYGPQALVDV SASDGCDTVV AAIVGAAGLA PSLAAARAGK RILLANKESL VMSGGIFMDA VRDHGAILLP VDSEHNAIFQ CMPRDENEHG GISKIILTAS GGPFRTREPA TLADVTPDEA CKHPNWVMGR KISVDSATMM NKGLEVIEAH WIFGLPGERI DVLIHPQSVI HSLVSYRDGS VLAQLGNPDM RTPIAHALAF PERVDAGVDQ LDLAQIAQLS FEKPDYARFP CLALALKALE EGGIASAALN AANEVAVEAF LERRIGFMAI AATVDAVLNA LPNRSPNGLD DVLAADADAR RLAAGFIAKA PAPRVERIV

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gcaaaaacgt ctgacactgc tcggttccac gggctcgatc ggagacagca cgctcgacgt ggtcgcccgc catcccgagc gtttttcggt gtacgcgctg accgcgcatc gcaacggcga caagctcgtc gagcaatgcc tgcgcttcgc gccggaagtc gcggtggtcg gcgacgcggc gaccgccgcg cacgtcgaag caaagctgcg cgccgcgggc agcaagacca cggtgctgta tggtccgcag gcgctcgtcg acgtgtcggc gagcgacggc tgcgacacgg tcgtcgcggc gatcgtcggc gcggccggcc tcgcgccgag cctcgcggcc gcgcgcgcgg gcaagcgcat cctgctcgcg aacaaggaat cgctggtgat gtcgggcggg atcttcatgg acgcggtgcg cgaccatggc gcgatcctgc tgccggtcga cagcgagcac aacgcgatct tccagtgcat gccgcgcgac gagaacgagc acggcggcat ctcgaagatc atcctgacgg cgtcgggcgg cccgttccgc acgcgcgagc cggccacgct cgccgacgtg acgccggacg aggcgtgcaa gcatccgaac tgggtgatgg gccgcaagat ctccgtcgat tcggcgacga tgatgaacaa gggcctcgag gtgatcgagg cgcactggat cttcggtctg ccgggcgagc ggatcgacgt gctgatccac ccgcagagcg tgatccattc gctggtgtcg tatcgcgacg ggtcggtgct cgcgcagctc ggcaaccccg acatgcgtac gccgatcgcg cacgcgctcg cgttcccgga gcgcgtcgac gccggcgtcg atcagctcga cctcgcgcag atcgcgcagc tgtcgttcga gaagccggat tacgcacgct tcccgtgtct cgcgctcgcg ctgaaggcgc ttgaagaggg cgggatcgcg agcgccgcat tgaacgcggc gaacgaagtc gcggtcgaag cgtttctcga gcgccggatc ggcttcatgg cgatcgcggc gacggtcgac gcggtgctca acgcactgcc gaaccgcagc ccgaacgggc tcgacgacgt cctggcggcc gatgccgacg cgcgtcgtct tgccgccggg ttcattgcaa aagcgcctgc gcctcgcgtg gagcgtatcg tctaaacagc acgaacaagt tctgcagcca agcttctcga ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaa

Details for BuviA.01136.a.A1.PS01530

PURIFICATION DATe:	6/25/2012
CONCENTRATION:	27.8mg/ml
OBSERVED MW:	44kDa
EXPRESSION LEVEL:	Low Expression
PROTEIN PURIFICATION BUFFER:	20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	data unavailable
VIAL VOLUME:	data unavailable
PERCENT IDENTITY:	99
PERCENT COVERAGE:	63

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GCAAAAACGT CTGACACTGC TCGGTTCCAC GGGCTCGATC GGAGACAGCA CGCTCGACGT GGTCGCCCGC CATCCCGAGC GTTTTTCGGT GTACGCGCTG ACCGCGCATC GCAACGGCGA CAAGCTCGTC GAGCAATGCC TGCGCTTCGC GCCGGAAGTC GCGGTGGTCG GCGACGCGGC GACCGCCGCG CACGTCGAAG CAAAGCTGCG CGCCGCGGGC AGCAAGACCA CGGTGCTGTA TGGTCCGCAG GCGCTCGTCG ACGTGTCGGC GAGCGACGGC TGCGACACGG TCGTCGCGGC GATCGTCGGC GCGGCCGGCC TCGCGCCGAG CCTCGCGGCC GCGCGCGCGG GCAAGCGCAT CCTGCTCGCG AACAAGGAAT CGCTGGTGAT GTCGGGCGGG ATCTTCATGG ACGCGGTGCG CGACCATGGC GCGATCCTGC TGCCGGTCGA CAGCGAGCAC AACGCGATCT TCCAGTGCAT GCCGCGCGAC GAGAACGAGC ACGGCGGCAT CTCGAAGATC ATCCTGACGG CGTCGGGCGG CCCGTTCCGC ACGCGCGAGC CGGCCACGCT CGCCGACGTG ACGCCGGACG AGGCGTGCAA GCATCCGAAC TGGGTGATGG GCCGCAAGAT CTCCGTCGAT TCGGCGACGA TGATGAACAA GGGCCTCGAG GTGATCGAGG CGCACTGGAT CTTCGGTCTG CCGGGCGAGC GGATCGACGT GCTGATCCAC CCGCAGAGCG TGATCCATTC GCTGGTGTCG TATCGCGACG GGTCGGTGCT CGCGCAGCTC GGCAACCCCG ACATGCGTAC GCCGATCGCG CACGCGCTCG CGTTCCCGGA GCGCGTCGAC GCCGGCGTCG ATCAGCTCGA CCTCGCGCAG ATCGCGCAGC TGTCGTTCGA GAAGCCGGAT TACGCACGCT TCCCGTGTCT CGCGCTCGCG CTGAAGGCGC TTGAAGAGGG CGGGATCGCG AGCGCCGCAT TGAACGCGGC GAACGAAGTC GCGGTCGAAG CGTTTCTCGA GCGCCGGATC GGCTTCATGG CGATCGCGGC GACGGTCGAC GCGGTGCTCA ACGCACTGCC GAACCGCAGC CCGAACGGGC TCGACGACGT CCTGGCGGCC GATGCCGACG CGCGTCGTCT TGCCGCCGGG TTCATTGCAA AAGCGCCTGC GCCTCGCGTG GAGCGTATCG TCTAAACAGC ACGAACAAGT TCTGCAGCCA AGCTTCTCGA GGATCCGGCT GCTAACAAAG CCCGAAAGGA AGCTGAGTTG GCTGCTGCCA CCGCTGAGCA ATAACTAGCA TAACCCCTTG GGGCCTCTAA ACGGGTCTTG AGGGGTTTTT TGCTGAAAGG AGGAACTATA TCCGGATATC CACAGGACGG GTGTGGTCGC CATGATCGCG TAGTCGATAG TGGCTCCAAG TAGCGAAGCG AGCAGGACTG GGCGGCGGCC AAAGCGGTCG GACAGTGCTC CGAGAACGGG TGCGCATAGA AATTGCATCA ACGCATATAG CGCTAGCAGC ACGCCATAGT GACTGGCGAT GCTGTCGGAA TGGACGATAT CCCGCAAGAG GCCCGGCAGT ACCGGCATAA CCAAGCCTAT GCCTACAGCA TCCAGGGTGA CGGTGCCGAG GATGACGATG AGCGCATTGT TAGATTTCAT ACACGGTGCC TGACTGCGTT AGCAATTTAA CTGTGATAAA CTACCGCATT AAAGCTTATC GATGATAAGC TGTCAAACAT GAGAA