SSGCID: Target Page

BuviA.00863.a

Histidine kinase

CENTER ID:	BuviA.00863.a
ORGANISM:	Burkholderia vietnamiensis G4
ASSOCIATED DISEASE:
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	True
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BuviA.00863.a.A2.GE34872	Mature protein( BuviA.00863.a )		35	439

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|269482.11.peg.5493
RefSeq:	YP_001119104.1
UniProt:	A4JDB6

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MNIIWARFLV SLMLLVLISV LVGVFAGPIA GLAFAVVMLV AQGFFSTFHT QRLWRLLDAP VYGEVPSAPG IWGEIYYRLH KLAKQWHAQV RQVEQQHSRF IQAIQASPNG VAMLDDHDQI EWCNAIAEVH FGVDAKRDLR QHITNLVRHP EFVRYLNGQH YDETLVMRGM GESRQNVLEV QVFPYGENRK LLLTQDITEL ERTDAMRRDF VANVSHELKT PLTVLSGFLE TMRELPLNED DRARYLDLME QQASRMRHIV TDLLVLAKLE GESRPPTDHA VDMRALFDHL KEDAQTLSNG HHDITFSIDE TLGVTGAQTE VFSAFANLVT NAIRYTPDGG KIAVSWRREG AQGVFSVTDS GFGIPAADLP RLTERFYRVD RSRSRDTGGT GLGLAIVKHV LQRHDSHLYV QSEEGRGSTF TARFPASRVI AIRPAAFEA

NT Sequence

ATGAACATCA TCTGGGCGCG CTTTCTCGTG TCGCTCATGC TGCTCGTGCT GATCAGCGTA TTGGTCGGCG TGTTCGCCGG CCCGATTGCG GGCCTCGCGT TCGCGGTCGT GATGCTGGTT GCGCAGGGCT TCTTCAGTAC GTTCCACACG CAACGCCTGT GGCGTCTGCT CGATGCGCCC GTCTATGGCG AGGTGCCGAG CGCGCCGGGC ATCTGGGGCG AAATCTATTA CCGGCTGCAC AAGCTCGCGA AGCAGTGGCA TGCGCAGGTG CGCCAGGTCG AGCAGCAGCA TTCGCGCTTC ATCCAGGCGA TCCAGGCGTC GCCGAACGGC GTCGCGATGC TCGACGATCA CGACCAGATC GAATGGTGCA ACGCGATCGC CGAGGTGCAC TTCGGCGTCG ATGCGAAACG CGACCTGCGC CAGCACATCA CGAACCTCGT GCGCCATCCC GAGTTCGTCC GCTACCTGAA CGGGCAGCAT TACGACGAGA CGCTCGTGAT GCGCGGCATG GGCGAATCGC GGCAGAACGT GCTCGAAGTG CAGGTGTTTC CGTACGGCGA GAACCGCAAG CTGCTGCTCA CGCAGGACAT CACCGAGCTC GAGCGCACCG ATGCGATGCG GCGCGACTTC GTCGCCAACG TGTCACATGA GCTGAAGACG CCGCTGACGG TGCTGTCGGG CTTCCTCGAG ACGATGCGCG AGCTGCCGCT GAACGAAGAC GATCGCGCGC GCTATCTCGA CTTGATGGAG CAGCAGGCGT CGCGGATGCG GCATATCGTG ACCGATCTGC TGGTGCTCGC GAAGCTCGAG GGCGAAAGCC GGCCGCCGAC GGATCACGCG GTCGACATGC GCGCGCTGTT CGATCATCTG AAGGAGGACG CGCAGACGCT GTCGAACGGG CACCACGACA TCACGTTCTC GATCGACGAG ACGCTCGGCG TGACCGGCGC GCAGACCGAA GTGTTCAGCG CGTTCGCGAA TCTCGTCACC AATGCGATCC GCTATACGCC GGACGGCGGC AAGATCGCCG TGTCGTGGCG GCGCGAGGGC GCGCAAGGCG TGTTTTCGGT CACCGACAGC GGCTTCGGCA TTCCGGCGGC CGACCTGCCG CGACTGACCG AGCGCTTCTA CCGCGTCGAT CGCAGCCGCT CGCGCGACAC GGGCGGCACG GGGCTCGGCC TCGCGATCGT CAAGCACGTG CTGCAGCGGC ACGACTCGCA CCTGTACGTG CAGAGCGAGG AAGGGCGCGG CAGCACGTTC ACCGCGCGGT TCCCGGCGTC GCGCGTGATC GCGATCCGGC CCGCGGCGTT CGAGGCG

Details for BuviA.00863.a.A2.GE34872

HARVESTED ON:	4/17/2012
SEQUENCED ON:	4/17/2012
EXPECTED MW:	48kDa
OBSERVED MW:	45kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	96
PERCENT COVERAGE:	69

Validated AA Sequence

Validated NT Sequence

atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatggcgg tcgtgatgct ggttgcgcag ggcttcttca gtacgttcca cacgcaacgc ctgtggcgtc tgctcgatgc gcccgtctat ggcgaggtgc cgagcgcgcc gggcatctgg ggcgaaatct attaccggct gcacaagctc gcgaagcagt ggcatgcgca ggtgcgccag gtcgagcagc agcattcgcg cttcatccag gcgatccagg cgtcgccgaa cggcgtcgcg atgctcgacg atcacgacca gatcgaatgg tgcaacgcga tcgccgaggt gcacttcggc gtcgatgcga aacgcgacct gcgccagcac atcacgaacc tcgtgcgcca tcccgagttc gtccgctacc tgaacgggca gcattacgac gagacgctcg tgatgcgcgg catgggcgaa tcgcggcaga acgtgctcga agtgcaggtg tttccgtacg gcgagaaccg caagctgctg ctcacgcagg acatcaccga gctcgagcgc accgatgcga tgcggcgcga cttcgtcgcc aacgtgtcac atgagctgaa gacgccgctg acggtgctgt cgggcttcct cgagacgatg cgcgagctgc cgctgaacga agacgatcgc gcgcgctatc tcgacttgat ggagcagcag gcgtcgcgga ngcggcatat cgtgancgat ctgctggtgc tcgcgaagct cgagggcgaa agncggccgc cgacggatca cgcggtcgan ntgcgcgcgc tgttcnatca tctgaaggag ganncgcana cgctgtcnaa cgggcaccac gacntcacgt tctcgatcga c

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMAVVMLVAQ GFFSTFHTQR LWRLLDAPVY GEVPSAPGIW GEIYYRLHKL AKQWHAQVRQ VEQQHSRFIQ AIQASPNGVA MLDDHDQIEW CNAIAEVHFG VDAKRDLRQH ITNLVRHPEF VRYLNGQHYD ETLVMRGMGE SRQNVLEVQV FPYGENRKLL LTQDITELER TDAMRRDFVA NVSHELKTPL TVLSGFLETM RELPLNEDDR ARYLDLMEQQ ASRMRHIVTD LLVLAKLEGE SRPPTDHAVD MRALFDHLKE DAQTLSNGHH DITFSIDETL GVTGAQTEVF SAFANLVTNA IRYTPDGGKI AVSWRREGAQ GVFSVTDSGF GIPAADLPRL TERFYRVDRS RSRDTGGTGL GLAIVKHVLQ RHDSHLYVQS EEGRGSTFTA RFPASRVIAI RPAAFEA

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggcggtcgtg atgctggttg cgcagggctt cttcagtacg ttccacacgc aacgcctgtg gcgtctgctc gatgcgcccg tctatggcga ggtgccgagc gcgccgggca tctggggcga aatctattac cggctgcaca agctcgcgaa gcagtggcat gcgcaggtgc gccaggtcga gcagcagcat tcgcgcttca tccaggcgat ccaggcgtcg ccgaacggcg tcgcgatgct cgacgatcac gaccagatcg aatggtgcaa cgcgatcgcc gaggtgcact tcggcgtcga tgcgaaacgc gacctgcgcc agcacatcac gaacctcgtg cgccatcccg agttcgtccg ctacctgaac gggcagcatt acgacgagac gctcgtgatg cgcggcatgg gcgaatcgcg gcagaacgtg ctcgaagtgc aggtgtttcc gtacggcgag aaccgcaagc tgctgctcac gcaggacatc accgagctcg agcgcaccga tgcgatgcgg cgcgacttcg tcgccaacgt gtcacatgag ctgaagacgc cgctgacggt gctgtcgggc ttcctcgaga cgatgcgcga gctgccgctg aacgaagacg atcgcgcgcg ctatctcgac ttgatggagc agcaggcgtc gcggatgcgg catatcgtga ccgatctgct ggtgctcgcg aagctcgagg gcgaaagccg gccgccgacg gatcacgcgg tcgacatgcg cgcgctgttc gatcatctga aggaggacgc gcagacgctg tcgaacgggc accacgacat cacgttctcg atcgacgaga cgctcggcgt gaccggcgcg cagaccgaag tgttcagcgc gttcgcgaat ctcgtcacca atgcgatccg ctatacgccg gacggcggca agatcgccgt gtcgtggcgg cgcgagggcg cgcaaggcgt gttttcggtc accgacagcg gcttcggcat tccggcggcc gacctgccgc gactgaccga gcgcttctac cgcgtcgatc gcagccgctc gcgcgacacg ggcggcacgg ggctcggcct cgcgatcgtc aagcacgtgc tgcagcggca cgactcgcac ctgtacgtgc agagcgagga agggcgcggc agcacgttca ccgcgcggtt cccggcgtcg cgcgtgatcg cgatccggcc cgcggcgttc gaggcgtaaa cagcacgaac aagttctgca gccaagcttc tcgaggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaa