BuviA.00171.b

3-Oxoacyl-(Acyl-carrier-protein (ACP)) synthase III C terminal domain protein

CENTER ID: BuviA.00171.b
ORGANISM: Burkholderia vietnamiensis G4
ASSOCIATED DISEASE:
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BuviA.00171.b.A1.GE32436 Full length( BuviA.00171.b ) 1 345
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
RefSeq: YP_001114950.1
UniProt: A4JQZ2

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MRPSANFTVV EPHIVATARY IPQRDVPADA HLEPFMDSGG SAREAVMQAV YADTLRWRDS GAPFGVAGIP LPHTSRATNH RVAVESDLTL SDMALATARM LLDSSHQNAA YPLDQFIVCT TSFEHDLALS CACRLHSELG SSRAPLAVGQ LQGVSFLMAL DIAVAMMATD IRLNTVVIVA AERWRQPFSR RIGALVDMGD GAAAVLIARH PGPGWRVRGL TVRTPGIPPL ASEREPVYIS TLTEVIGETC ASAGVQPANV DWILPAPIDA HVARAIHRRC GLRHERACHL RMDAPGQFCA ADTPARLDAL LRSIHPNEGQ HALAWSAGFQ GQAGCALLEF RGGRS
NT Sequence
ATGCGTCCTT CGGCTAACTT CACGGTCGTC GAGCCGCACA TTGTCGCCAC GGCAAGATAT ATCCCACAGC GCGATGTCCC GGCCGATGCA CATCTCGAAC CATTCATGGA TTCGGGTGGG TCCGCACGAG AGGCTGTAAT GCAAGCCGTG TATGCCGACA CGCTGCGGTG GCGCGACAGC GGAGCCCCAT TCGGCGTGGC CGGTATTCCA TTGCCGCACA CATCACGAGC GACGAATCAC CGGGTCGCTG TGGAGTCTGA CCTGACATTA TCCGACATGG CACTTGCCAC GGCGCGAATG CTACTCGATT CATCGCATCA GAACGCTGCG TACCCGCTCG ATCAGTTCAT CGTATGCACA ACGAGCTTCG AGCATGATCT AGCATTATCG TGTGCCTGCC GTCTCCACAG CGAACTCGGA TCAAGCCGAG CCCCGCTCGC GGTCGGTCAG CTTCAGGGCG TATCGTTCCT GATGGCATTG GACATTGCCG TGGCAATGAT GGCAACTGAT ATCCGACTGA ATACCGTCGT GATTGTCGCT GCTGAACGCT GGCGTCAGCC GTTCTCACGT CGGATCGGTG CATTGGTCGA CATGGGTGAC GGGGCAGCTG CCGTACTGAT CGCGCGACAC CCCGGCCCGG GATGGCGCGT GAGGGGGCTC ACCGTCCGGA CGCCCGGAAT CCCACCTCTC GCAAGTGAAC GCGAACCCGT CTACATATCG ACGCTCACGG AGGTCATCGG CGAGACGTGC GCAAGCGCAG GTGTGCAACC TGCCAATGTC GACTGGATCC TTCCGGCGCC AATCGACGCT CATGTGGCTC GCGCAATCCA CAGGCGATGT GGATTGCGGC ACGAACGTGC CTGCCACCTA CGCATGGACG CGCCCGGCCA ATTTTGCGCC GCCGACACGC CGGCACGGCT CGACGCGCTG CTGCGGTCGA TCCACCCTAA CGAGGGACAA CATGCCCTTG CTTGGTCCGC CGGCTTCCAA GGGCAGGCCG GTTGCGCTCT CCTTGAATTT CGTGGAGGTC GATCA
Details for BuviA.00171.b.A1.GE32436
HARVESTED ON: 11/8/2011
SEQUENCED ON: 11/11/2011
EXPECTED MW: 39kDa
OBSERVED MW: 40kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 94
PERCENT COVERAGE: 92
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMRPSANFTV VEPHIVATAR YIPQRDVPAD AHLEPFMDSG GSAREAVMQA VYADTLRWRD SGAPFGVAGI PLPHTSRATN HRVAVESDLT LSDMALATAR MLLDSSHQNA AYPLDQFIVC TTSFEHDLAL SCACRLHSEL GSSRAPLAVG QLQGVSFLMA LDIAVAMMAT DIRLNTVVIV AAERWRQPFS RRIGALVDMG DGAAAVLIAR HPGPGWRVRG LTVRTPGIPP LASEREPVYI STLTEVIGET CASAXVQPAN VDWILPAPID AHVARAIHRR CGLRHERACH LRMDAPXPIL XRRXAGTARR AAAVDXP
Validated NT Sequence
ttttgttnnn tttaagaagg agatatacca tggctcatca ccatcaccat catatgggta ccctggaagc tcagacccag ggtcctggtt cgatgcgtcc ttcggctaac ttcacggtcg tcgagccgca cattgtcgcc acggcaagat atatcccaca gcgcgatgtc ccggccgatg cacatctcga accattcatg gattcgggtg ggtccgcacg agaggctgta atgcaagccg tgtatgccga cacgctgcgg tggcgcgaca gcggagcccc attcggcgtg gccggtattc cattgccgca cacatcacga gcgacgaatc accgggtcgc tgtggagtct gacctgacat tatccgacat ggcacttgcc acggcgcgaa tgctactcga ttcatcgcat cagaacgctg cgtacccgct cgatcagttc atcgtatgca caacgagctt cgagcatgat ctagcattat cgtgtgcctg ccgtctccac agcgaactcg gatcaagccg agccccgctc gcggtcggtc agcttcaggg cgtatcgttc ctgatggcat tggacattgc cgtggcaatg atggcaactg atatccgact gaataccgtc gtgattgtcg ctgctgaacg ctggcgtcag ccgttctcac gtcggatcgg tgcattggtc gacatgggtg acggggcagc tgccgtactg atcgcgcgac accccggccc gggatggcgc gtgagggggc tcaccgtccg gacgcccgga atcccacctc tcgcaagtga acgcgaaccc gtctacatat cgacgctcac ggaggtcatc ggcgagacgt gcgcnagcgc angtgtgcaa cctgccaatg tcgactggat ccttccggcg ccaatcgacg ctcatgtggc tcgcgcaatc cacaggcgat gtggattgcg gcacgaacgt gcctgccacc tacgcatgga cgcgcccngg ccaattttgn gccgccgann cgcnggcacg gctcgacgcg ctgctgcngt cgatcnnccc taacgaggga nanntgncnn ngntngncnn ngntggnnng cnnnnnncnc tnnnnanttn nngngncgat cntnananan nantnngcan nnnctnnnnn nnnncnnnnc tnnannnnnn nnnnngnnnn nnnnnca
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMRPSANFTV VEPHIVATAR YIPQRDVPAD AHLEPFMDSG GSAREAVMQA VYADTLRWRD SGAPFGVAGI PLPHTSRATN HRVAVESDLT LSDMALATAR MLLDSSHQNA AYPLDQFIVC TTSFEHDLAL SCACRLHSEL GSSRAPLAVG QLQGVSFLMA LDIAVAMMAT DIRLNTVVIV AAERWRQPFS RRIGALVDMG DGAAAVLIAR HPGPGWRVRG LTVRTPGIPP LASEREPVYI STLTEVIGET CASAGVQPAN VDWILPAPID AHVARAIHRR CGLRHERACH LRMDAPGQFC AADTPARLDA LLRSIHPNEG QHALAWSAGF QGQAGCALLE FRGGRS
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gcgtccttcg gctaacttca cggtcgtcga gccgcacatt gtcgccacgg caagatatat cccacagcgc gatgtcccgg ccgatgcaca tctcgaacca ttcatggatt cgggtgggtc cgcacgagag gctgtaatgc aagccgtgta tgccgacacg ctgcggtggc gcgacagcgg agccccattc ggcgtggccg gtattccatt gccgcacaca tcacgagcga cgaatcaccg ggtcgctgtg gagtctgacc tgacattatc cgacatggca cttgccacgg cgcgaatgct actcgattca tcgcatcaga acgctgcgta cccgctcgat cagttcatcg tatgcacaac gagcttcgag catgatctag cattatcgtg tgcctgccgt ctccacagcg aactcggatc aagccgagcc ccgctcgcgg tcggtcagct tcagggcgta tcgttcctga tggcattgga cattgccgtg gcaatgatgg caactgatat ccgactgaat accgtcgtga ttgtcgctgc tgaacgctgg cgtcagccgt tctcacgtcg gatcggtgca ttggtcgaca tgggtgacgg ggcagctgcc gtactgatcg cgcgacaccc cggcccggga tggcgcgtga gggggctcac cgtccggacg cccggaatcc cacctctcgc aagtgaacgc gaacccgtct acatatcgac gctcacggag gtcatcggcg agacgtgcgc aagcgcaggt gtgcaacctg ccaatgtcga ctggatcctt ccggcgccaa tcgacgctca tgtggctcgc gcaatccaca ggcgatgtgg attgcggcac gaacgtgcct gccacctacg catggacgcg cccggccaat tttgcgccgc cgacacgccg gcacggctcg acgcgctgct gcggtcgatc caccctaacg agggacaaca tgcccttgct tggtccgccg gcttccaagg gcaggccggt tgcgctctcc ttgaatttcg tggaggtcga tcataaacag cacgaacaag ttctgcagcc aagcttctcg aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaa